Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Dietary Lactobacillus brevis Supplementation on Growth Performance, Dry Matter and Nitrogen Digestibilities, Blood Cell Counts and Fecal Odor Emission Compounds in Growing Pigs

육성돈사료에 Lactobacillus brevis의 첨가가 성산성, 건물과 질소 소화율, 혈구수 및 분 내 악취 발생 물질에 미치는 영향

  • Published : 2006.08.31
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Abstract

This study was conducted to investigate the effects of dietary Lactobacillus brevis (3.4×108 CFU/g) supplementation on growth performance, DM and N digestibilities, blood cell counts and fecal odor emission compounds in growing pigs. Ninety six crossbred [(Landrace×Yorkshire)×Duroc] pigs with an initial BW of 24.60±1.28kg were used for 42-d feeding trial according to a completely randomized design. Three corn- soybean meal based dietary treatments included: 1) CON (basal diet); 2) LB1 (basal diet + Lactobacillus brevis 0.2%) and 3) LB2 (basal diet+Lactobacillus brevis 0.4%). There were three dietary treatments with eight replicate pens per treatment and four pigs per pen. Through the entire experimental period, ADG, ADFI and gain/feed had no significant differences among treatments(P>0.05). Nitrogen digestibility was increased in LB1 and LB2 treatments compared to CON treatment (linear effect, P<0.05), however, DM digestibility had no significant difference among all the treatments (P>0.05). The WBC, RBC and lymphocyte concentrations in whole blood were not affected by treatments (P>0.05). Fecal NH3N and H2S concentrations were significant decreased in LB2 treatment compared to CON treatment (linear effect, P<0.05). Fecal VFA (acetic acid and propionic acid) concentration was also reduced in LB2 treatment compared to CON treatment (linear effect, P<0.05). In conclusion, Lactobacillus brevis (3.4×108 CFU/g) supplementation at the level of 0.4% can improve nitrogen digestibility and decrease the concentrations of fecal odor emission compounds in growing pigs.

본 시험은 육성돈 사료내 생균제 (Lactobacillus brevis, 3.4×108 CFU/g)의 첨가, 급여가 생산성, 건물과 질소 소화율, 혈구수 및 분 내 악취 발생 물질에 미치는 영향을 조사하기 위하여 실시하였다. 개시시 체중 24.60±1.28kg의 3원교잡종 [(Landrace×Yorkshire)×Duroc] 육성돈 96두를 공시하여 42일간 사양시험을 실시하였다. 시험설계는 옥수수－대두박 위주의 사료내 생균제를 첨가하지 않은 CON (basal diet), 생균제를 0.2% 첨가한 LB1 과 생균제를 0.4% 첨가한 LB2의 3개 처리구로 하여 처리당 8반복, 반복당 4두씩 완전임의 배치하였다. 전체 시험기간동안의 일당증체량, 일당사료섭취량 및 사료효율에서 있어서는 처리구간 유의한 차이를 나타내지 않았다(P<0.05). 질소 소화율에서 LB1 과 LB2 처리구가 대조구와 비교하여 유의적으로 증가하였다(linear effect, P<0.05). 그러나 건물 소화율에 있어서는 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 혈액내 WBC, RBC 및 lymphocyte 함량에 있어서는 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 분내 암모니아태 질소 및 황화수소의 함량은 LB2 처리구가 대조구와 비교하여 유의적으로 감소하였다(linear effect, P<0.05). 분내 acetic acid 와 propionic acid 함량에서는 BMS2 처리구가 대조구와 비교하여 유의적으로 감소하였다(linear effect, P<0.05), butyric acid 에서는 각 처리구간 유의적인 차이는 없었다(P>0.05). 결론적으로, 육성돈 사료내 0.4%의 Lactobacillus brevis (3.4×108 CFU/g) 첨가는 질소 소화율 향상 및 분내 악취 발생 물질 함량을 감소 시키는 것으로 사료된다.

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References

  1. Aattouri, N., Bouras, M., Tome, D., Marcos, A. and Lemonnier, D. 2002. Oral ingestion of lactic acid bacteria by rats increases lymphocytic proliferation and interferon. production. Br. J. Nutr. 87:367-373 https://doi.org/10.1079/BJN2001527
  2. AOAC. 1995. Official method of analysis. 16th Edition. Association of Official Analytical Chemists. Washington. DC
  3. Apgar. G. A.. Kornegay. F. T.. Lindemann. M. D. and Wood. C. M. 1993. The effect of feeding various levels of Bifidobacteriurn globosurn A on the performance. gastrointestinal measurements. and immunity of weanling pigs and on the performance and carcass measurements of growing-finishing pigs. J. Anim, Sci. 71:2173-2179
  4. Avery. G. L. Merva. G. E. and Gerrish. J. B. 1975. Hydrogen sulfide production in swine confinement units. Trans. ASAE. 17: 149-151
  5. Baird. D. M. 1977. Probiotics help boost feed efficiency. Feedstuffs. 49:11-12
  6. Banwart. W. L. and Bremner. J. M. 1975. Formation of volatile sulfur-compounds by microbial decomposition of sulfur-containing amino acids in soils. Soil. Biol. Biochem. 7:3590-364
  7. Bomba. A.. Nemcova, R.. Gancarcikova, S.. Herich. R.. Guba, P. and Mudronova, D. 2002. Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids. British Journal of Nutrition. 88 (Suppl.) 1: 95-99 https://doi.org/10.1079/BJN2002634
  8. Burgstaller. G., Ferstl, R. and Apls, H. 1984. The addition of lactic acid bacteria (Streptococcus faecium SF-68) to a milk replacer for calf feeding. Zuchtungskunde. 56: 156-162
  9. Chaney, A. L. and Marbach, E. P. 1962. Modified regents for determination of urea and ammonia. Clin. Chem. 8: 131
  10. Chen. Y. J., Son, K. S., Min, B. J.. Cho, J. H., Kwon, O. S. and Kim. I. H. 2005. Effects of dietary probiotic on growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content in growing pigs. Asian-Aust. J. Anim. Sci. 18:1464-1468 https://doi.org/10.5713/ajas.2005.1464
  11. Chen. Y. J., Min, B. J., Cho, J. H., Kwon, O. S., Son. K. S.. Kim. I. H. and Kim. S. J. 2006. Effects of dietary Enterococcus faecium SF68 on growth performance. nutrients digestibility. blood characteristics and fecal noxious gas content in finishing pigs. Asian-Aust. J. Anim. Sci. 19:406-411 https://doi.org/10.5713/ajas.2006.406
  12. Collins. M. D. and Gibson. G. R. 1999. Probiotics, prebiotics and synbiotics: approaches for modulating the microbial ecology of the gut. Anim. J. Clin. Nutr. 69 (suppl):1052-1057 https://doi.org/10.1093/ajcn/69.5.1052s
  13. Elina, R.. Erja, M.. Maria S.. Merja. R.. Johannes. A. and Airi. P. 2003. Probiotic and milk technological properties of Lactobacillus brevis. Int. J. of Food Microbiol. 83:63-74 https://doi.org/10.1016/S0168-1605(02)00315-X
  14. Elsden. S. R.. Hitchcock. M. W. S.. Marshall. R. A. and Phillipson. A. T. 1946. Volatile acid in the digesta of ruminants and other animals. J. Exp. Biol. 22: 191-202
  15. Franklin. M. A.. Mathew, A. G.. Vickers. J. R. and Clift. R. A. 2002. Characterization of microbial populations and volatile fatty acid concentrations 111 the jejunum. ileum. and cecum of pigs weaned at 17 vs 24 days of age. J. Anim. Sci, 80:2904-2910
  16. Fuller. R. 1989. Probiotics in man and animals. J. Appl. Bacteriol, 66:365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  17. Hale. O. M. and Newton, G. L. 1979. Effects of a nonviable Lactobacillus species fermentation product on performance of pigs. J. Anim. Sci, 48:770
  18. Han. I. K.. Lee, J. H .. Piao. X. S. and Li, D. F. 2001. Feeding and management system to reduce environmental pollution in swine production: A review. Asian-Aust. J. Anim. Sci. 14:432-444 https://doi.org/10.5713/ajas.2001.432
  19. Hays. V. W. 1969. Use of Drugs in Animal Feeds. National Academy of Science. Washington. D. C
  20. Heber. A. J.. Duggirala R. K.. Ni. J. Q.. Spence. M. L.. Haymore. B. L.. Adamchuk, V. I.. Bundy. D. S.. Sutton. A. L.. Kelly. D. T. and Keener. K. M. 1997 Manure treatment to reduce gas emissions from large swine houses. Vinkeloord, The Netherlands. pp: 449-458
  21. Heilig. H. G. H. J.. Zoetendal. F. G .. Vaughan. E. E.. Marteau. P.. Akkermans. A. D. L and de Vos. W. M. 2002. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl. Environ. Microbiol. 68:114-123 https://doi.org/10.1128/AEM.68.1.114-123.2002
  22. Hong. J. W.. Kim. I. H.. Kwon, O. S .. Kim. J. H.. Min. B. J. and Lee. W. B. 2002. Effects of dietary probiotics supplementation on growth performance and fecal gas emission in nursmg and finishing pigs. J. Anim. Sci & Technol. (Kor.) 44:305-314 https://doi.org/10.5187/JAST.2002.44.3.305
  23. Imoto. S. and Namioka, S. 1978. VFA production in the pig large intestine. J. Anim. Sci. 47:467-478 https://doi.org/10.2527/jas1978.472467x
  24. Ji. F. and Kim. S. W. 2002. Reducing odor in swine production: Effect of enzymes and probiotics on ammonia production. J. Anim. Sci. Vol. 80 (Suppl. I)
  25. Jasek. S. R.. Kalinowska, R.. Knecht. D. and Pawiak, R. 1992. Effect of Biogen probiotic addition on reproduction results and physiological indices in pigs. Rocz. Nauk. Zootech. 31 :239
  26. Jonsson. E. and Conway, P. 1992. Probiotics for pigs. In: R. Fuller (Ed.) Probiotics: The Scientific Basis. Chapman & Hall. London. pp:260-316
  27. Kadota, H. and Ishida. Y. 1972. Production of volatile sulfur compounds by microorganisms. Ann. Rev. Microbiol. 26:127-138 https://doi.org/10.1146/annurev.mi.26.100172.001015
  28. Kil, D. Y.. Lim, S. J.. Tian, J. Z.. Kim, B. G.. Kim, K. S. and Kim. Y. Y. 2004. Effect of continuous feeding of probiotics on growth performance, nutrient digestibility. blood urea nitrogen and immune responses in pigs. J. Anim. Sci & Technol. (Kor.) 46:39-48 https://doi.org/10.5187/JAST.2004.46.1.039
  29. Kornegay. E. T., Wood. C. M.. Ball, G. G. and Risley. C. R. 1990. Use of Lactobacillus acidophilus for growing and finishing pigs. VA Polytech. Inst. State Univ. Anim. Sci. Res. Rep. 9:13
  30. Maxwell. C. Y.. Buchanan. D. S.. Owens. F. N.. Gilliland. S. E.. Luce. W. G. and Vend. R. 1983. Effect of probiotic supplementation on performance, fecal parameters and digestibility In growing finishing swine. Oklahoma Agric. Exp. Sta.. Anim. Sci. Res. Rep. 114:157
  31. NRC. 1998. Nutrient requirement of pigs. 10th Edition. National Research Council. Academy Press. Washington, D. C
  32. Otto. E. R., Yokoyama, M.. Hengernuehle, S.. von Bermuth, R. D., van Kempen, T. and Trottier. N. L. 2003. Ammonia, volatile fatty acids. phenolics, and odor offensiveness in manure from growing pigs fed diets reduced in protein concentration. J. Anim. Sci. 2003. 81:1754-1763
  33. Peterson, R. G. 1985. Design and Analysis of Experiments. Marcel Dekker. New York
  34. Sandine, W. E. 1979. Role of lactobacillus in the intestinal tract. J. Food Protect. 42: 259-262 https://doi.org/10.4315/0362-028X-42.3.259
  35. SAS. 1996. SAS user's guide. Release 6.12 edition. SAS Institute. Inc Cary NC. USA
  36. Shon, K. S., Hong, J. W., Kwon, O. S., Min, B. J., Lee, W. B., Kim, I. H., Park, Y. H. and Lee, I. S. 2005. Effects of Lactobacillus reuteri-based direct-fed microbial supplementation for growing-finishing pigs. Asian-Aust. J. Anim. Sci. 18:370-374 https://doi.org/10.5713/ajas.2005.370
  37. Spriet, S. M.. Decuypere. .J. A. and Henderickx. H. K. 1987. Effect of Bacillus toyoi (Toyocerin) on the gastrointestinal microflora. concentration of some bacterial metabolites. digestibility of the nutrients and the small intestinal mean retention time in pigs. Meded. Fac. Landbouwkd. Rijksuniv. Gent. 52: 1673
  38. Tortuero. F.. Rioperez, J.. Fernandez, E. and Rodriguez. M. L. 1995. Response of piglets to oral administration of lactic acid bacteria. J. Food Protect. 58:1369-1374 https://doi.org/10.4315/0362-028X-58.12.1369
  39. Wenk, C. 2000. Recent advances in animal feed additives such as metabolic modifiers, antimicrobial agents, probiotics, enzymes and highly available minerals. Asian-Aust, J. Anim. Sci. 13:86-95 https://doi.org/10.5713/ajas.2000.86
  40. Zahn, J. A., Hatfield, J. L., Do, Y. S., DiSpirito, A. A.. Laird, D. A. and Pfeiffer. R. L. 1997. Characterization of volatile organic emissions and wastes from a swine production facility. J. Environ. Qual. 26: 1687 -1696 https://doi.org/10.2134/jeq1997.2661687x