• 보존과학연구
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• s.32
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• pp.5-24
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• 2011

Neunghwaji(Embossed patterned paper) is a unique paper used for a traditional book cover in Korea. The research was carried out to investigate Neunghwaji's features. Physical property was studied through a test of tensile strength and folding endurance. Also, comparative analysis of virus resistance and waterproof ability was undertaken on Neunghwaji. 1. Folding endurance test showed that strength of non-embossed CB and HB decreased during deteriorating duration. Embossed CN and HN showed the strength increasing at early stage and decreasing from the 27th day of the deteriorating duration. Tensile strength was decreasing in both cases as deterioration progressed. 2. Growth of Arthrinium sp. fungus on embossed paper was 10% less than plain paper while Cladosporium sp. showed 20~30% less growth. Amur cork dyeing(H) showed 10~30% lower fungi growth than Gardenia seed dyeing(C). The result indicated that embossed paper has better virus resistance than Hanji, and Amur cork dyeing has better virus resistance than Gardenia seed dyeing. 3. Average contact angle of CN, CB, HN, and HB was $85{\sim}92^{\circ}$ and NON-N and NON-B was $59{\sim}63^{\circ}$. In detail, CN's contact angle was $1{\sim}7^{\circ}$ higher than CB's; HN was $1{\sim}6^{\circ}$ higher than HB. Therefore, it was found that embossed paper has higher contact angle than Hanji thus the former has better waterproof ability. The research suggested production technique of Neunghwaji and studied its features related to the technique. Neunghwaji was confirmed to have superior quality to Hanji though further study regarding above test result is needed to complement the research.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.972-978
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• 2005

The present study was carried out to investigate the blood markers of Jeju horses. The redcell cypes (blood groups) and blood protein types (biochemical polymorphisms) were tested from 102 Jeju horses by serological and electrophoretc procedure, and their phenotypes and gene frequencies were estimated. The blood group and biochemical polymorphism phenotypes observed with high frequency were $A^{af}\;(27.45\%$), $C^{a}\;(99.02\%$), $K^{-}\;(97.06\%$), $U^{a}\;(62.75\%$), $P^{b}\;(36.27\%$), $Q^{c}\;(47.06\%$), $D^{cgm/dghm}\;(13.73\%$), $D^{adn/cgm}\;(9.80\%$), $D^{ad/cgm}$\;(8.82\%$), $D^{dghm/dghm}(7.84\%$), $D^{cgm/cgm}(7.84\%$), $AL^{B}\;(48.04\%$), $GC^{F}\;(99.02\%$), $AlB^{K}\;(97.06\%$), $ES^{FI}\;(36.27\%$), $TF^{F2}\;(25.49\%$), $HB^{B1}\;(45.10\%$), and $PGD^{F}\;(86.27\%$) in Jeju horses, respectively. Alleles observed with high gene frequency were $A^{af}$ (0.3726), $A^{C}$ (0.2647), $C^{-}$ (0.5050), $K^{-}$ (0.9853), $U^{-}$ (0.6863), $P^{b}$ (0.4657), $Q^{c}$ (0.5294), $D^{cgm}$ (0.3039), $HB^{B1}$(0.6863), $PGD^{F}$ (0.9265), $AL^{B}$ (0.6912), $ALB^{K}$ (0.9852), $GC^{F}$ (0.9950), $ES^{I}$ (0.5000) and $TF^{F2}$ (0.4950) in Jeju horses, and sfecific alleles, $D^{cgm(f)}$ (0.0196), $HB^{A}$ (0.0147), $HB^{A2}$ (0.0196), $ES^{G}$ (0.0441), $ES^{H}$ (0.0098), $TF^{E}$TF'(0.0246), $TF^{H2}$ (0.0049) and $PGD^{D}$ (0.0098) were detected in Jeju horses. These preliminary results present basic information for detecting the genetic markers in Jeju horse. and developing a system for parentage verification and individuals identification in jeju horses.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.461-471
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• 2016

This study was conducted to establish an in vitro propagation system for sea-milkwort (Glaux maritima L.), which is an endangered coastal plant species with high horticultural value. Two phenotypes, 'Red type (RT)' and 'Pistachio type (PT)' based on the colors of stem and flower, were obtained from a personal horticulturist in 2009 and used for this study as plant materials. The stock plants showed typical morphologies in flower, capsule, and seed appearances as previously reported. Low temperature treatment at $4^{\circ}C$ for four or more weeks after in vitro sowing maximized seed germination percentage, indicating that imbibition of seed and subsequent low temperature treatment are crucial for its germination. The in vitro seedlings had phenotypic variation, falling into 'RT' and 'PT' classes like the stock plants. Although slight differences depending on genotype and medium were recognized, the fourth or fifth nodes detached from the in vitro seedlings revealed the best multiplication efficacy when estimated on the basis of total number of nodes of newly developed axillary shoots. In addition, the nodes from 'RT' and 'PT' regenerated the most shoots on medium supplemented with $0.5mg{\cdot}L^{-1}$ BA alone and $0.5mg{\cdot}L^{-1}$ BA plus $0.5mg{\cdot}L^{-1}$ IAA, respectively. The node culture-derived plantlets were well acclimatized in a culture room ex vitro and completed the pseudo-annual life cycle coincident with that in the natural salt march habitat with the current cultivation method of applying fresh water-irrigation under an inland environment. This work represents the first report of in vitro propagation of sea-milkwort. Thus, our study will contribute to exo-habitat conservation and natural habitat restoration of this endangered species in addition to development of a horticultural product.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.675-686
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• 2015

Asparagus (Asparagus officinalis L.) needs proper post-harvest treatment to prolong its storage life. This study investigated the effect of 1-methylcyclopropene (1-MCP) on the quality and storage life of asparagus. Fresh-harvested asparagus was treated with 1-MCP ($1mg{\cdot}L^{-1}$), CEPA($10mg{\cdot}L^{-1}$), and 1-MCP($1mg{\cdot}L^{-1}$) + CEPA($10mg{\cdot}L^{-1}$) and compared with an untreated control. The carbon dioxide ($CO_2$) production, ethylene production, and morphological characteristics of the preserved asparagus were observed. The flow-system and the static-type measurement methods for ethylene and $CO_2$ production (respiration rate) were used. Weight loss, respiration rate, degree of freshness, and ethylene production were monitored during storage at $7^{\circ}C$. The results further showed that CEPA (2-chloroethylphosphonic acid) treatment had greater effects on $CO_2$ and ethylene production than using the 1-MCP process. The asparagus treated with CEPA or 1-MCP + CEPA had significantly increased the ethylene production rate compared to the control or using only 1-MCP during storage. There were no evident changes in the respiration rate of asparagus under 1-MCP treatment as compared with the control. Using the flow-system, slight differences in the rates of $CO_2$ and ethylene production were noted as compared to using the static type. Findings showed that in using the flow-system, asparagus manifested clearer results as compared with the static type. Weight loss in asparagus was significantly lower in control and 1-MCP treated samples than in those treated with CEPA. Likewise, the $CO_2$ and ethylene production of the CEPA treated samples significantly increased. The 1-MCP treatment reduced the effects of CEPA on weight loss, soluble solids content, and osmolality. The effect was not observed with exogenous ethylene as CEPA treatment had no visible effect as compared to the untreated group. Thus, 1-MCP treatment of asparagus could slightly reduce damage to the quality of asparagus during its distribution where ethylene gas is produced. Therefore, this study suggests that 1-MCP treatment can reduce the damage induced by ethylene gas on asparagus in poor distribution environments.

• Korean journal of food and cookery science
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• v.19 no.2
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• pp.195-209
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• 2003

The purpose of this study was to identify the hazard analysis critical control point on food production and transportation flow, applied to home-delivered meals for the elderly. To carry out this study, 1) pan-fried oak mushroom and meat, soy sauce glazed hair tail, and roasted dodok were selected as high nutrient and preferred foods for the elderly and 2) time, temperature, and microbiological quality(standard plate count, coliform, Salmonella spp, Vibrio parahaemolyticus, Staphylococcus spp., Escherichia coli O157:H7, and Listeria monocytogenes) were measured at various phases of the home-delivered meal production and its transportation flows. The results of this experiments are as follows: The temperature measured at cooling phases during the home-delivered meal production flows was 19.2 ∼ 20.0$^{\circ}C$ for the pan- fried oak mushroom and meat and the roasted dodok and was 24.0 ∼ 25.2$^{\circ}C$ for the soy sauce glazed hair tail. These temperature were in the potentially dangerous zone. Microbiological analysis showed that S. spp. was higher in the raw ingredients, including oak mushroom, hair tail, radish, and dodok, than the standard limit. SPC was lower than the standard limit from cooking to transportation phase, but SPC increased significantly during the cooling and packaging phase. The level of coliform detected was far lower than the standard limit and was not detected at all during the transportation phase. Few S. spp. was detected in the pan-fried oak mushroom and meat, but was found in above standards limit during the wrap packaging phase in the soy sauce glazed hair tail and roasted dodok. The level decreased rapidly during the holding and transportation phase. Sal. spp., V. parahaemolyticus, S. spp., E. coli O157:H7, and L. monocytogenes were not detected. For the pan-fried oak mushroom and meat, the critical control points were during the purchasing and receiving of raw ingredients, cooling, and packaging phases. For the soy sauce glazed hair tail and roasted dodok, the critical control points were during the purchasing and receiving of raw ingredients, preparation, cooling, and packaging phases.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.916-923
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• 1998

To provide more information on the enteric pathogen Campylobacter jejuni in the view of food sanitation, survival characteristics of two strains of C. jejuni in the different conditions were investigated. When $10^7{\;}or{\;}10^3{\;}per{\;}ml$ of C. jejuni cells were inoculated in the supplemented Brucella broth and kept at $42^{\circ}C,{\;}25^{\circ}C{\;}and{\;}5^{\circ}C$ under the static aerobic condition for 7 days, organisms exponentially proliferated to $a{\;}>10^8$, even in the $10^3{\;}per{\;}ml$ inoculated-sample at $42^{\circ}C{\;}for{\;}1{\sim}2{\;}days$ and the considerable level of viability maintained during 7 days. At $5^{\circ}C$, most of the initial level of organisms survived at the early period and only $a{\;}<{\;}0.5-log_{10}$ cells decrease were observed during the 7 days. At $25^{\circ}C$, a remarkable number of C. jejuni declined within $1{\sim}2{\;}days$ and showed undetectable level of cells after 4 days. When sterile milk and minced chicken meat were artifically inoculated with $10^7{\;}per{\;}ml$ of C. jejuni, mostly, a $1-to{\;}2-log_{10}$ count decrease occurred at $42^{\circ}C{\;}and{\;}5^{\circ}C$ while $a{\;}>3{\;}log_{10}$ decrease at $25^{\circ}C$ during 7 days. Unexpectedly, no colonies appeared on the plate inoculated from the minced chicken meat sample kept at $42^{\circ}C$ after 4 days. The results suggest that C. jejuni contaminated to food can survive at the refrigeration temperature whereas they are sensitive to at the room temperature. Also, it is shown that the growth of C. jejuni at the optimal temperature may vary to the food sources.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.61-69
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• 2015

This study was carried out to investigate the effects of shipping temperature and harvesting stage on the quality and vase life of cut flower in standard chrysanthemum 'Baekma' for export. Cut flowers harvested at flowering stage 4 were stored for 24 hours at $5^{\circ}C$, and then quality and vase life were evaluated after simulated shipping for 48 hours at 5, 20, and $35^{\circ}C$. In addition, cut flowers harvested in flowering stage 1 to stage 6 were evaluated after simulated shipping for 72 hours at $5^{\circ}C$. As shipping temperature increased, $CO_2$ concentration inside the box rapidly increased, and $O_2$ concentration greatly decreased. The vase life of cut flowers was extended by 3 days and chlorophyll content of leaves, fresh weight, solution uptake, and flower diameter were better maintained in $5^{\circ}C$ shipping than in 20 or $35^{\circ}C$ shipping. The vase life of cut flowers harvested at flowering stage 1 or 2 was extended by 5.2 or 5.5 days compared to those harvested at flowering stage 6, more 6. The fresh weight was lower and flower diameter was smaller by 1.3 or 2.5 cm in cut flowers of flowering stages 1 through 3 than flowering stages 4 through 6. In addition, the cut flowers of flowering stages 4 through 6 showed higher solution uptake than those of flowering stages 1 through 3. These results suggest that shipping at $5^{\circ}C$ of cut flowers harvested at flowering stage 4 is preferential for promoting vase life and quality of cut flower in standard chrysanthemum 'Baekma' for export to Japan.

• Current Research on Agriculture and Life Sciences
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• v.5
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• pp.119-126
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• 1987

This study was conducted to elucidate the most appropriate method to obtain auxotrophic mutants from Valsa ceratosperma, the causal fungus of apple canker, which may be used as a gene marker in detecting the transfer of the factors of avirulent strains to virulent strains. Among the 3 kinds of synthetic media tested, each have two formula for minimal and complete, the medium which has been used in study of Endothia parasitica (E. P medium) was turned out to be most appropriate for the growth of V. ceratosperma. A medium for single colony formation from pycnidiospore of this fungus was developed by adding 0.5% L - sorbose to the E. P minimal medium. The period of incubation in dark for preventing the photoreactivation after U. V irradiation was estimated as about 60hrs at which most of the spores become binucleate. Largest number of putative auxotrophs were obtained at about 50second of irradiation to the spores smeared on the medium for single colony formation, at which the survival rate of spores was 5 to 6 percent. With these method developed in this experiment, 161 isolates of putative auxotrophs were detected among which the nutrient requirement for 10 isolates were determined. Five out of 10 mutants were still virulent to apple tree and all but one could not sporulate.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.3
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• pp.214-220
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• 1993

Browning of ascidian, Halocynthia roretzi, meat occurres very rapidly when skinned off or cut during processing and it resulted the quality loss of fresh frozen, dehydrated or fermented products. In this study, the causes of color development and prevention of browning were experimented. The browning of ascidian meat may be occurred enzymatically by a tyrosinase contained in meat and viscera which acted specifically on L-tyrosine as a substrate rather than on catechol. Activity of the enzyme in viscera was three times higher than in meat. The optimum pH and temperature on the tyrosinase activity of crude enzyme obtained from ascidian was 6.0 and $30{\sim}35^{\circ}C$, respectively. The enzyme was inactivated heating at $80^{\circ}C$ for 3 minutes or $90{\sim}100^{\circ}C$ for 1 minute and it was inhibited by $0.1{\sim}0.5mM$ solutions at ascorbic acid, sodium hydrogen sulfite, cystein, citric acid, cyanide but only sodium hydrogen sulfite treatment was effective to retard such a high content of enzyme as in case of viscera. In practical use for processing of ascidian meat browning was retarded by dipping the viscera removed ascidian meat in 0.2M citric acid for 5 minutes or $0.2\%$ sodium hydrogen sulfite solution for 1 minute resulting in sulfur dioxide residue less than 100 ppm.

• Journal of Korean Society of Forest Science
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• v.79 no.2
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• pp.144-161
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• 1990

Pine forest communities developed in Mt. Palgong, southeastern Korea, were studied phytosociologically, with special reference to multiple management of local forests, and were classified into two communities, Pinus densiflora - Quercus mongolica community(I : mountain forest) and P. densiflora-Climbing plants community (II : valley forest) and six subgroups accompanied by several subgroups. Judging from the coincidence method, the division of communities (vegetation units) was closely related to altitude and topography. Based on vegetation units, a vertical distribution map of pine forest communities was prepared. The species composition(%) of pine forest communities under stratification, in upper and lower tree layer, teas I higher than II, in middle and shrubs lacer II higher than I (Total : Upper 15.5%, Middle 28.4%, Lower 34.6%. Shrubs 21.5%. Sum of mean coverage%i of understory vegetation was II twice as high as I (Total. shrubs 28.4%. forbs 11.4%, Graminoids 11.8%, ferns 1.0%). Based on constance, coverage and d.b.h. class etc., an actual growth and occurrence table of tree species and understory vegetation by vegetation unity were prepared, and could assume a criterion for judging potential dominance-growth conditions.