• KSBB Journal
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• v.11 no.1
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• pp.8-14
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• 1996

Bacillus subtillis strains with transition state regulator mutations and a spore mutation were developed for the overexpression of apsE and for the enhancement of expression level. Among the many regulator genes, degU and hpr were chosen as a representative positive and negative regulator for the aprE, respectively. Spo II G was used for the construction of asporogeneous strains. All the mutants were constructed from two protease-deleted strain DB104 and the apsE gene was transformed with an integration vector pMK101. DB104(deg$U^h$(32) $his^+$)::pMK101(Cm) and DB104($\Delta$her(Em))::pMKl01(Cm) show 7-fold and about 2-fold increase in aprE expression level, respectively. But the effect of transition state regulator mutation on the aprE expression was diminished when the integrated aprE gene was amplified by the high concentration of chloramphenicol, i. e. 30 $\mu\textrm{g}$/ml. DB104($\Delta$spoIIG(Pm) degUh(32) his+)::pMK101(Cm) and DB104($\Delta$spoIIG(Pm) $\Delta$hpr(Em))::pMK101 double mutant show 10-fold and 3-fold increase in aprE expression level, respectively. The results suggest that sporulation mutation and transition state regulator mutation have independent and additive effect on the aprE expression, and the same gene dosage effect on the transition state regulator mutation was also identified.

• Journal of Life Science
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• v.9 no.6
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• pp.728-732
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• 1999

The inhibitory effects of methanol extracts from wild mushrooms on mutagenicity induced by benzo(a)pyrene (B(a)P) and N-methyl-N-nitro- nitrosoguanidine(MNNG) were investigated using Salmonella typhimurium reversion assay. In Ames test, The methanol extracts of 11 mushrooms did not show any mutagenicity but The methanol extracts of Lactarius piperatus, Naematoloma fasciculare and Innotus xeranticus showed 40∼80% of inhibitory effect on the mutagenicity induced by indirect mutagen of B(a)P and also showed 60∼80% of antimutagenic activity toward MNNG irrespective of their concentrations.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.127-127
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• 2003

벼 도열병 저항성 조절과정을 연구하는 효율적인 방법은 돌연변이를 분리하여 해당유전자를 분석하는 것이다. 이를 위하여 벼에서 제조된 4가지 돌연변이 집단을 이용하여 도열병 저항성 돌연변이를 분리하였다. 실험재료로는 1) fast neturon을 처리하여 제조된 Moroberekan 2,000 라인, 2)T-DNA의 형질전환에 의해서 제조된 화영 1,000 라인, 3)DEB처리에 의해서 제조된 RIL260 3,000라인, 4) gamma ray 처리에 의해서 제조된 상해향혈나 10,000 라인 등이 사용되었다. 병 저항성이 감소된 돌연변이의 분리를 위하여 재료로 사용된 벼 품종과 비친화적 상호작용을 보이는 균주의 포자를 2-3주된 벼 잎에 직접 살포하는 방법을 이용하였다. 균주 접종 7 일 후에 blast lesion을 형성하거나 lesion mimic 표현형을 보이는 돌연변이 등 병저항성이 감소된 라인을 선별하였다. 현재까지 1) Moroberakan 5 라인, 2) 화영 4 라인, 3) RIL260 1 라인 등이 선별되었다. 이와 함께 병저항성이 현저히 증가된 돌연 변이를 선별하기 위하여 친화적인 균주를 사용한 실험에서는 상해향렬나 2 라인이 선별되었다. 선별된 돌연변이는 벼 도열병 저항성 유전자의 분리 및 저항성 조절기작을 연구하는데 효과적으로 사용될 것이다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.4C
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• pp.424-432
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• 2003

In this paper, an effective method of stereo image reconstruction through the regularized adaptive disparity estimation is proposed. Althougth the conventional adaptive disparity estimation method can sharply improve the PSNR of a reconstructed stereo image, but some problems of overlapping between the matching windows and disallocation of the matching windows can be occurred, because the matching window size changes adaptively in accordance with the magnitude of feature values. Accordingly, in thia paper, a new regularized adaptive disparity estimation technique is proposed. That is, by regularizing the estimated disparity vector with the neughboring disparity vectors, problems of the conventional adaptive disparity estimated scheme might be solved, and also the predicted stereo image can be more effectively reconstructed. From some experiments using the CCETT'S stereo image pairs of 'Man' and 'Claude', it is analyzed that the proposed disparity estimation scheme can improve PSNRs of the reconstructed images to 10.89dB, 6.13dB for 'Man' and 1.41dB, 0.81dB for 'Claude' by comparing with those of the conventional pixel-based and adaptive estimation method, respectively.

• KSBB Journal
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• v.15 no.4
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• pp.331-336
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• 2000

The antimutagenic activity of the extract of Artemisia capillaris THUNB on the mutagenicity induced by benzo(a)pyrene [B(a)P] in the presense of S9 mixture was studied using bacterial mutagenic assay system. Samples harvested in summer and autumn were extracted using ethanol and hot water. Among these extracts the water extract of summer sample had the strongest inhibitory effect against the mutagenenicity of B(a)P, The water extract of Artemisia capillaris THUNB was separated again into ethanol soluble and insoluble parts. The ethanol insoluble part(El) of water extract exhibited higher inhibition effects than the ethanol soluble part against the mutagenic activity of B(a)P. El showed dose-dependent activity on the mutagenicity of B(a)P in SOS Chromotest and Ames test. The 50% inbibition concentraction $(IC_{50}$ of El were $200{\mu}g/assay$ $600{\mu}g/plate$ and $800{\mu}b/plate$ in E. coil PQ37 S. typhimurium TA100 and TA98 respectively. El were showed desmutagenic effect but had no effect on the DNA repair system for B(a)P-induced mutagenesis. HPLC analysis showed that the formation of aflatoxin M1 by cytochrome P-450 1A1 known as playing an impotant role on B(a) P-induced mutagenicity was highly inhibited by El. Therefore we encluded that B(a)P-induced mutagencity can be reduced possible due to the interference of el with cytochrome P-450 1A1-dependent bioactivation.

• Proceedings of the KSRS Conference
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• 2006.03a
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• pp.169-172
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• 2006

본 논문에서는 1m 해상도의 위성영상으로부터 건물의 경계선을 검출하기 위해 영상분할과 변이지도(disparity map)를 이용하는 새로운 방법을 제안한다. Watershed 방법으로 영상을 분할하고 분할된 영역 내부의 변이를 다중정합창틀(multiple matching window)과 결합된 다차원특징벡터정합(multi-dimensional feature vector matching)을 이용하여 계산한다 분할된 인접 영역들 가운데 panchromatic 및 multispectral 밝기값과 변이의 평균값이 유사하면 두 영역을 결합하여 하나의 영역을 생성하고 이 과정을 반복적으로 수행한다. 영역의 평균 변이값이 기준 값보다 크면 이를 건물 지붕 영역으로 결정한다. IKONOS 위성영상에 제안한 방법을 적용하여 작은 건물이 밀집되어 있는 도시 지역에서 건물 지붕의 영역과 경계선을 효과적으로 검출할 수 있었다.

• Korean Journal of Remote Sensing
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• v.10 no.1
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• pp.15-29
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• 1994

Radiometric correction refers to variations in the data that are not caused by the object or scene being scanned. These variations can be caused by differing sensitivities of the detectors of the sensing system, malfunctioning detectors, or atmospheric interference. Radiometric corrections can be applied to correct for these variations, such as for differing sensitivities of detectors (causing striped image), for detectors (resulting in pixels with digital values of zero), or to correct for atmospheric bias due to scattering of radiation. This paper discussed and illustrated some of the important principles of the radiometric correction methods.

• Korean Journal of Food Science and Technology
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• v.28 no.6
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• pp.1059-1064
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• 1996

The inhibitory effects of chitosan on mutagenicity induced by 3-amino-1-methyl-5H-pyrido [4,3-b] indole (Trp-P-2), sodium azide (SA), 2-nitrofluorene (2-NF), and 4-nitroquinoline oxide (4-NQO) were investigated using Salmonella typhimurium reversion assay and SOS Chromotest. In Salmonella typhimurium reversion assay. Chitosan showed 24-65% of inhibitory effect against the mutagenicity of an indirect-acting mutagen, Trp-P-2. On the other hand, no inhibitory effect was observed against the mutagenicity of direct-acting mutagens (2-NF, SA). In SOS chromotest. chitosan showed 46-49% effects on SOS function induced by 4-NQO. Chitosan inhibited the mutagenicity induced by Trp-P-2 with 9-39% of inhibition rate. It was also evaluated whether inhibitory effect of chitosan is due to its bio-antimutagenic or desmutagenic action. Chitosan at high concentrations showed a bio-antimutagenicity with dose-dependent manner, but it showed a desmutagenicity at low concentrations against the mutation induced by Trp-P-2.

• Journal of the Korea Convergence Society
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• v.10 no.12
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• pp.517-523
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• 2019

The aim of the study was to examine the effects of the five-sensory horticulture therapy on perceived stress and HRV in adults with depression. The participants were 26 (experimental group=13, control group=13) adult with depression and enrolled in the Regional C mental health welfare center from April to June 2018. The experimental group participated in a total of 8-session five sensory horticultural therapy once a week, and the control group provided horticultural therapy once to the desired subjects after the program. The data were analyzed using descriptive statistics, t-test, and Mann-Whitney U test. There were statistically significant change in perceived stress (t = 3.11, p = .005), LF (t = -3.39, p = .002) and SDNN (t = -2.48, p = .025) in experimental group compared to the control group. Therefore, this therapy was effective for reduction of stress among individuals with the depressive disorder.

• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.450-455
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• 1998

Pine has been known as a traditional medicinal plant and as showing a physically beneficial function to a human being. Therefore, this study was performed to investigate the physiological activities of main pine neddles. Ethanol extracts from pinus needles did net exhibit any mutagenicity. On the contrary, inhibitory effects of ethanol extract were observed on mutagenicity induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), 4-nitroquinoline-1-oxide (4NQO), 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1) and benzo(a)pyrene $(B({\alpha})P)$ using Salmonella typhimurium reversion assay. On direct-acting mutagen (MNNG, 4NQO) and indirect-acting mutagen (Trp-P-1, $(B({\alpha})P)$, we observed higher inhibitory effect. Stepwise fractionation of the ethanol extract was done by using ether, chloroform, ethyl acetate, butanol and water to obtain effective fraction. Among them, water fractions $(100\;{\mu}g/plate)$ of Pinus thunbergii, Pinus rigida, Pinus densiflora and Pinus koraiensis showed high inhibition of 91.65%, 94.7%, 84.22% and 79.02%, respectively, on the mutagenicity of MNNG in Salmonella typhimurium TA100.