• Title/Summary/Keyword: 변이종

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Adult Trapping using Sex Attractants of Grapholita molesta, Carposina sasakii and Conogethes punctiferalis mixed in Single Dispensers (단일방출제에 섞인 혼합 성페로몬 미끼를 이용한 복숭아순나방, 복숭아심식나방, 복숭아명나방의 성충 포획)

  • Jung, Jin Kyo;Choi, Kyung Hee;Han, Kyung Sik;Lee, Soon Won;Boo, Kyung Saeng;Cho, Young Sik
    • Korean journal of applied entomology
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    • v.60 no.1
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    • pp.71-78
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    • 2021
  • Trapping efficacy of lures obtained by mixing sex pheromone attractants of two or three species in a single dispenser were evaluated in peach and apple orchards for three lepidopteran fruit borers, Grapholita molesta (GM), Carposina sasaki (CS), and Conogethes punctiferalis (CP) which use different chemicals as their sex attractants. In the peach orchard, the trapping efficacy of the mixed lures of GM and CS attractants were reduced for GM trapping, compared with the single GM lure. The population fluctuation patterns of GM were highly correlated between the single lure and all the mixed lures. In the apple orchard, the efficacy of all the mixed lures were not reduced for GM trapping. The trapping efficacy of all the mixed lures for CS trapping were not reduced compared with the CS single lure in both the peach and apple orchards. In the peach orchard, the population fluctuation patterns of CS were highly correlated between the single lure and the mixed lures. The efficacy of the mixed lures for CP trapping could not be determined because of the small number of caught samples. The results indicated that mixed lures could be utilized for monitoring of the annual adult emergence of two species, GM and CS.

Starch Content and Its Associated Grain Characters in Naked Barley (쌀보리 품종의 전분함량 및 종실관련형질)

  • Park, Moon-Woong;Chae, Je-Cheon;Kim, Jung-Gon;Lee, Choon-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.55-62
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    • 1994
  • The germplasms of naked barley evaluated for starch content were 137 crossing blacks and progeny lines obtained from the breeding program of the Wheat and Barley Research Institute, 540 Tabdong Kwonsungu Suwon, in the 1988/1989 season. Starch content was distributed between 49.15 and 62.50% with a mean of 57.20%. It was high in the bred lines and the Uz type lines than the imported, uz type or lax type ones. The range observed for seed coat weight was 1.12-3.02mg and the mean seed coat weight was 1.88mg. The seed coat weight ratio to kernel weight was measured between 4.0 and 8.7% with a mean of 6.5%. The varieties differ greatly in the characteristics depending on the plant type and origin of germplasms. The variation observed with protein content ranged from 9.60 to. 15.90% while the average was 12.86%. Starch content was negative relationships with kernel weight, seed coat weight, seed coat weight ratio., kernel width, kernel thickness and protein content, and starch content was higher in smaller kernels within a variety. The weight and weight ratio. of seed coat were positively correlated with the width, thick-ness of kernels, whilst they were negatively correlated with starch content. The heritability of starch content was 30.0%.

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Characterization of Mutations in Bruton's Tyrosine Kinase(Btk) Gene from Unrelated 3 X-linked Agammaglobulinemia(XLA) Families in Korea (국내 X-관련성 범저감마글로불린혈증 세가족에 대한 Bruton's Tyrosine Kinase 단백질 발현 및 유전자 변이 분석)

  • Song, Chang-Hwa;Jo, Eun-Kyeong;Park, Jeong-Kyu;Kim, Jung-Soo;Hong, Soo-Jong;Lee, Jae-Ho
    • Clinical and Experimental Pediatrics
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    • v.45 no.3
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    • pp.302-310
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    • 2002
  • Purpose : X-linked agammaglobulinemia(XLA) is an immunodeficiency caused by abnormalities in Bruton's tyrosine kinase(Btk), and is characterized by a deficiency of peripheral blood B cells. We studied cytoplasmic expression of Btk protein and analyzed the Btk gene in peripheral blood mononuclear cells(PBMC) from three XLA families in Korea. Methods : Heparinized venous blood samples were collected from four XLA patients and additional family members in three unrelated XLA families. Mononuclear cells were separated from their blood and the intracellular Btk protein was characterized by a flow cytometry. The mutation analysis was performed using direct sequencing. Results : Cytoplasmic expression of Btk protein in monocytes was not detected in the patients with XLA. We observed a novel deletion and two point mutations within introns(intron 1 and intron 18) resulting in alternative splicings. In XLA family 2, a 980 bp deletion(from intron 9+191 T to intron 10-215 C) including exon 10 was found in patient P2. He was the only sporadic case in this study, because his mother and brother showed a normal Btk expression by flow cytometry. Conclusion : These identified genetic alterations support the molecular heterogeneity of Btk gene in XLA disease. Additionally, by means of flow cytometric analysis, we diagnosed three hypogammaglobulinemia patients as XLA. Advancements in diagnostic methods has facilitated a prompt and definite diagnosis of this disease.

Association Between the Polymorphism on Intron 5 of the Lipoprotein Lipase Gene and Carcass Traits in Hanwoo (Korean cattle) (한우 Lipoprotein Lipase 유전자 Intron 5번의 Polymorphism과 경제 형질과의 관련성 분석)

  • Lee, H.J.;Lee, S.H.;Cho, Y.M.;Yoon, H.B.;Jeon, B. K.;Oh, S.J.;Kwon, M.S.;Yoon, D.H.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.947-956
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    • 2004
  • The primary role of lipoprotein lipase(LPL) is the hydrolysis of triglycerides(TG) from the core of triglyceride-rich lipoproteins such as chylomicrons and very low density lipoproteins in plasma. Fatty acids liberated by LPL on capillary endothelial surfaces are available for tissues as energy sources especially in muscles or for storage in the form of TG in adipose tissues. Therefore, as the candidate gene related to the carcass traits of the beef cattle, we have directly sequenced the exon 5${\sim}$exon 6 region in the bovine LPL gene for discovery of single nucleotide polymorphism(SNP) with 24 unrelated Hanwoo(Korean cattle). Novel eight sequence variants were detected: three loci on exon 5, three on intron 5 and two on exon 6. All SNPs identified were strongly linked each other, and one hundred twenty eight Hanwoo samples were genotyped one SNP on intron 5 using PCR-restriction fragment length polymorphism method by digestion with Hae III restriction enzyme. The allele frequency of the polymorphism was 0.76 and 0.24. The effects of this polymorphism on the breeding values of the carcass weight, loin muscle area, back fat thickness and marbling score were analyzed using least square methods of SAS GLM. The marbling score of BB genotype was significantly higher than those of AA and AB genotypes(P<0.05). This result indicates that this polymorphism may be associated with the variation of marbling score. Further study is warranted to investigate the phenotypic association in Hanwoo.

Symptomatic Benign Intraosseous Osteolytic Lesions of the Glenoid: Report of 3 cases (증상이 있는 관절와의 양성 골내 골용해성 병변: 3예에 대한 증례보고)

  • Kim, Young Kyu;Cho, Seung Hyun;Moon, Sung Hoon
    • Clinics in Shoulder and Elbow
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    • v.16 no.1
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    • pp.40-46
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    • 2013
  • Benign intraosseous osteolytic lesions of the glenoid are very rare. The present study reports on three cases of symptomatic intraosseous osteolytic lesions of the glenoid in which surgical interventions were made. Of the three, two cases presented with intraosseous ganglion and one case with fibrous dysplasia. In all the cases, the lesion was located at the posteroinferior portion of the glenoid, and it seems to be related to posterior shoulder pain. If intraosseous osteolytic lesions have symptoms or the risk for chondral defects or cortical breakage, surgical intervention is needed and bone curettage with or without bone grafting will be a useful treatment option.

Molecular Phylogeny of the Family Strigidae (Aves) Based on Mitochondrial Cytochrome b Gene (Mitochondrial Cytochrome b 유전자에 의한 올빼미과 (Family Strigidae)의 분자계통)

  • 류시현;박희천
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.297-304
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    • 2003
  • Phylogenetic analysis of 31 species representing 12 genera in the family Strigidae (Aves: Strigiformes) including 5 species (Bubo bubo, Otus sunia, O. semitorques, Ninox scutulato, Strix aluco) collected from Korea has been undertaken using nucleotide sequences of the mitochondrial cytochrome b gene. Maximum likelihood analysis was performed and pairwise genetic distances were calculated with Kimura's two-parameter and p-distance. Among well-aligned 959 bp used for this study, 459 sites were variable and 398 sites were informative for the phylogenetic analysis. The family Strigidae was divided into three subgroups, Clade I (Aegolius), Clade II (Athene, Micrathene, Glaucidium and Surnia) and Clade III (Bubo, Nycteo, Pulsatrix, Strix, Otus, Ptilopsis, and Ninox). Also, two separated subgroups in the genus Otus were confirmed by the geographical distribution.

Rifampicin과 Ofloxacin에 내성인 정장용 세균의 개발

  • 최응칠;정영자;김숙경;김병각
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.252-252
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    • 1994
  • 현재 시판되고 있는 정장용 생균 제제에 함유되어있는 정장균주의 하나인 Bifidohacterium bifidum은 항결핵제 중 rifampicin에 감수성으로 rifimpicin과 병용 투여시 본래의 정장 효과를 기대할 수 없다. 따라서, rifampicin에 내성인 돌연변이 균주를 얻기 위해 B. bifidum을 N-methyl-N'-nitro-N-nitroso- -guanidine(MNNG)로 처리하여 rifampicin에 내성인 30종의 균주를 선별 하였고, rifampicin에 대한 Minimal Inhibitory Concentration(MIC)를 측정해 본 결과 내성이 1,000배 이상 상승하였다. 또한 rifampicin에 내성인 균주 RFR61을 자연 돌연변이시켜 ofloxacin에도 내성인 돌연변이 균주 20종을 선별 하였고, MIC를 측정한 결과 내성이 4배 이상 증가하였다. 또, fructose-6-phosphate phosphoketolase test를 실시해 본 결과, 모두 Bifidobacterium임이 확인되었다. 유기산 생성량을 측정하여 모균주의 유기산 생성량과 가장 유사한 3균주, B. bifidum RFRll, RFR21, RFR61 그리고 OFR9을 선별하였다. 이 네 균주의 E. coli 생육 억제능을 측정한 결과 모두 모균주와 유사한 E. coli 생육 억제능을 가지고 있었다. Rifampicin 내성균주들에 대하여 내성 유지 시험을 한 결과 복귀 돌연변이에 의해 내성이 소실될 가능성은 없는 것으로 여겨진다. 마지막으로, 내성 균주에 의한 rifampicin 불활성화 여부를 알아 본 결과 rifampicin이 불활성 화되지 않음을 알 수 있었다. 이상의 결과를 통해 본 연구실에서 개발한 B. bifidum RFR11, RFR21 그리고 RFR61 균주들은 rifampicin에 내성이며, B.bifidum OFR9은 rifampicin과 ofloxacin에 이중 내성을 갖는 균주로서 모균주와 유사한 생화학적 특성을 갖는 우수한 정장 세균으로 여겨진다.

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Comparative Anatomy of Secondary Xylem in Normal and Dwarf Individuals of Some Wood Plants (수 종의 목본식물에 있어서 정상 및 왜소개체의 이기목부의 비교해부)

  • 임동옥
    • Journal of Plant Biology
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    • v.34 no.1
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    • pp.9-18
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    • 1991
  • This study deals with the effect of dwarf growth on xylem structure, especially on the dimension of xylem elements described for 12 species of naturally occuring dwarf trees. The length and tangential diameter of tracheary elements and fibers in dwarf trees appear to be shorter and narrower than those in normal trees. Radial width and cell number of the same annual rings are narrower and smaller in dwarf trees than those in normal trees. Height of rays in dwarf trees is lower than that in normal trees. Dwarf conifers appear to have higher ratio, of latewood to earlywood than that in normal trees. In the hardwood species studied, mesomorphy of vessel elements is lower in dwarfs than that in normal trees. It can be concluded that this dwarf growth occurs as a result of extremely slow growth by environmental stress such as water deficiency.ciency.

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Leaf Area of Korean Local Soybean Collection (재래종 수집대두계통의 엽면적)

  • Shin-Han Kwon;Kun-Hyuk Im;Jae-Ri Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.19
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    • pp.47-51
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    • 1975
  • Leaf area and its relationships with other important agronomic traits were investigated with 727 lines of Korean local soybeans. Distributions of the leaf area, length, leaf width and LAI of the lines were shown in normal curves. Leaf area was positively associated with leaf length, and width, plant height, and seed size. Leaf form is rather affected by leaf width than leaf length.

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Isozyme Variation in Two Species of Freshwater Pleurocerid Snails in Korea: Koreanomelania nodifila and Koreoleptoxis globus ovalis (한국산 다슬기과 (Family Pleuroceridae) 2 종의 등위효소 변이)

  • 이준상;고정호;권오길
    • The Korean Journal of Malacology
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    • v.17 no.2
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    • pp.117-123
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    • 2001
  • A genetic analysis using starch gel electrophoresis was peformed to clarify the degrees of genetic differentiation among two species of the genus Koreanomelania and Koreoleptoxis (Gastropoda, Pleuroceridae). The average genetic similarity coefficients (S) among the populations of K. nodifila was S=0.848 (0.805-0.905) and K. globus ovalis was S : 0.755 (0.666-0.860). The genetic similarity coefficients between Semisulcospira gottschei and K. nodifila, S. gottschei and K. globus ovalis, and K. nodifila and K. giobus ovalis were 0.194, 0.301, and 0.301, respectively. So between K. nodifila and K. globus ovalis seems to be genetically two distinct taxa.

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