Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Association Between the Polymorphism on Intron 5 of the Lipoprotein Lipase Gene and Carcass Traits in Hanwoo (Korean cattle)

한우 Lipoprotein Lipase 유전자 Intron 5번의 Polymorphism과 경제 형질과의 관련성 분석

  • Lee, H.J. (National Livestock Research Institute, RDA, Department of Genetic Engineering, Sungkyunkwan University, Genetic Engineering Laboratory) ;
  • Lee, S.H. (National Livestock Research Institute, RDA) ;
  • Cho, Y.M. (National Livestock Research Institute, RDA) ;
  • Yoon, H.B. (National Livestock Research Institute, RDA) ;
  • Jeon, B. K. (National Livestock Research Institute, RDA) ;
  • Oh, S.J. (National Livestock Research Institute, RDA) ;
  • Kwon, M.S. (Department of Genetic Engineering, Sungkyunkwan University, Genetic Engineering Laboratory) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA)
  • 이한주 (농촌진흥청 축산연구소, 성균관대학교 유전공학과) ;
  • 이승환 (농촌진흥청 축산연구소) ;
  • 조용민 (농촌진흥청 축산연구소) ;
  • 윤호백 (농촌진흥청 축산연구소) ;
  • 전봉균 (농촌진흥청 축산연구소) ;
  • 오성종 (농촌진흥청 축산연구소) ;
  • 권무식 (성균관대학교 유전공학과) ;
  • 윤두학 (농촌진흥청 축산연구소)
  • Published : 2004.12.31
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Abstract

The primary role of lipoprotein lipase(LPL) is the hydrolysis of triglycerides(TG) from the core of triglyceride-rich lipoproteins such as chylomicrons and very low density lipoproteins in plasma. Fatty acids liberated by LPL on capillary endothelial surfaces are available for tissues as energy sources especially in muscles or for storage in the form of TG in adipose tissues. Therefore, as the candidate gene related to the carcass traits of the beef cattle, we have directly sequenced the exon 5${\sim}$exon 6 region in the bovine LPL gene for discovery of single nucleotide polymorphism(SNP) with 24 unrelated Hanwoo(Korean cattle). Novel eight sequence variants were detected: three loci on exon 5, three on intron 5 and two on exon 6. All SNPs identified were strongly linked each other, and one hundred twenty eight Hanwoo samples were genotyped one SNP on intron 5 using PCR-restriction fragment length polymorphism method by digestion with Hae III restriction enzyme. The allele frequency of the polymorphism was 0.76 and 0.24. The effects of this polymorphism on the breeding values of the carcass weight, loin muscle area, back fat thickness and marbling score were analyzed using least square methods of SAS GLM. The marbling score of BB genotype was significantly higher than those of AA and AB genotypes(P<0.05). This result indicates that this polymorphism may be associated with the variation of marbling score. Further study is warranted to investigate the phenotypic association in Hanwoo.

생물체의 체내 지방대사에 아주 중요하게 작용하는 LPL의 유전자 구조변이가 한우의 경제형질에 미치는 효과를 구명하고자, 사람 등 포유류에서 주요한 변이부위로 인식되어 온 LPL유전자의 exon 5${\sim}$exon 6 영역에서 구조변이를 탐색하였다. 부모가 각기 다른 한우 24두를 이용하여 PCR 증폭산물 1674 bp (exon 5${\sim}$exon 6)에서 총 8 좌위의 SNP 검출하였는데, 이는 SNP 검출율이 약 1SNP/210bp로 기존 SNP 검출율 보다 비교적 높은 비율이며, 검출된 SNP들 간에 95% 이상의 높은 연관(linkage) 관계를 보여 비교적 잘 보존되어 있는 영역으로 사료된다. 그리고 검출된 SNP를 PCR-RFLP 기법을 이용하여 표현형질 기록치를 확보한 한우 33차 후대검정축 129두의 유전자형을 결정하였다. 그 결과 intron 5번의 제한효소 Hae III로 처리한 823A\longrightarrowG 변이부위가 측정된 모든 도체형질에서 유전자형에 따라 뚜렷한 차이를 보였으며, 특히 근내지방도와 통계적 유의성이 인정되었다(p<0.05). 사람 및 생쥐에서 LPL의 촉매활성부위를 암호화하는 exon 5번 및 6번에서의 변이는 LPL의 활성도에 영향을 미치며, 이는 혈액내의 중성지방농도 및 지방대사에 작용한다는 보고가 있다. 이들 변이구조와 95% 이상 강한 연관을 보이는 intron 5번의 구조변이는 근내지방도와 유의적으로 관찰되었다. 앞으로, intron 5번의 823A\longrightarrowG 변이가 어떤 근거로 근내지방도와 유의적으로 나타났는지 그 근거를 증명할 수 있는 추가적인 실험이 필요한 것으로 사료된다.

Keywords

References

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