Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Complete Mitochondrial Genome Sequence and Genetic Diversity of Duroc Breed

돼지 Duroc 품종에서 미토콘드리아 유전체 서열의 특성과 집단의 유전적 다양성

  • Cho, 1.C. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Han, S.H. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Choi, Y.L. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Ko, M.S. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Lee, J.G (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.H (Division of Animal Science and Resources, Chungnam National University) ;
  • Jeon, J .T (Division of Applied Life Science, Gyeongsang National University)
  • 조인철 (농촌진흥청 난지농엽연구소) ;
  • 한상현 (농촌진흥청 난지농엽연구소) ;
  • 최유림 (농촌진흥청 난지농엽연구소) ;
  • 고문석 (농촌진흥청 난지농엽연구소) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 이준헌 (충남대학교 통물자원학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2004.12.31
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Abstract

Duroc is widely used to improve the meat quality and productivity. To elucidate the phylogenetic relation and the sequence specificity for the maternal property, the complete sequence of mitochondrial genome was determined and the population diversity of Duroc was investigated in this study. The length of mtDNA tested is 16,584-bp. There are several insertion/deletion mutations in the control region and coding regions for tRNA and rRNA, respectively, but not in peptide-coding regions. Four peptide-coding genes(COⅡ, COⅢ, ND3 and ND4) showed incomplete termination codon sequences such as T--, and two(ND2 and ND4L) did alternative initiation codons(AIC), respectively. Especially, the initiation codon sequences of ND2 gene were polymorphic in this population. Polymorphisms were detected in 11-bp duplication motif within control region as well as ND2 and CYTB. Variation patterns observed from the tests on three mtDNA regions were linked completely and then two haplotypes obtained from combining the data dividing this population. Duroc mtDNA is observed at the European pig cluster in the phylogenetic tree, however, the results from the population analyses supported previous opinions. This study suggests that the breed Duroc was mainly originated from the European pig lineage, and Asian lineage was also used to form the pig breed Duroc as maternal progenitors.

Duroc 품종은 돼지 사육에 있어 산육성과 육질 향상을 위해 이용되고 있다. 본 연구는 육종에 많이 이용되는 Duroc 품종의 모계 특이적인 서열의 검색과 계통유전학적 유연관계의 정립을 위하여 미토콘드리아 유전체의 전체 염기서열을 결정하고 집단 내 다형성을 조사하였다. mtDNA 전체 서열의 길이는 16,584-bp 이고, D-loop과 tRNA, rRNA 유전자 영역에서는 삽입/결실이 확인되었다. 4개의 coding gene (COⅡ, COⅢ, ND3, ND4)에서 불완전한 종결코돈을, ND4L과 ND2 유전자는 선택적 개시코돈 양상을 보였다. Duroc 집단에 대한 분석 결과 조절영역에서의 특이적인 11-bp 중복 단위가 일부 개체(15.2%)에서 발견되었고, ND2의 개시코돈과 CYTB 유전자에서도 다형현상을 보였다. 각각의 유전자 영역에서의 다형성은 서로 연관되어 있었고, 그 결과 Duroc 집단은 크게 두 가지 haplotype으로 구분되었다. 계통수에서 Duroc mtDNA 서열은 유럽계열 cluster에 위치하였으나, haplotype 분석과 기존에 연구결과들을 종합해 보면 Duroc 품종은 여러 모계선조 집단에서 기원한 것으로 보이며, 유럽과 아시아 계열 모두가 품종 형성에 이용된 것으로 사료된다된 것으로 사료된다.

Keywords

References

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