• Korean Journal of Animal Reproduction
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• v.18 no.4
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• pp.265-274
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• 1995

가토 20마리의 혈액중 혈소판의 수를 수정 전후로 조사한 결과, 수정전과 비교하여 수정후 1일부터 유의하게 감소하였으며 (26.84%, P<0.01), 이러한 감소는 수정후 5일까지 계속되었다. 수정후 5일동안 혈소판의 감소율과 혈소판의 최저치를 수정란의 수와 비교한 바, 이들간의 유의한 상관관계(r=0.5032)가 있었다. 가토수정란 (2세포기) 각각 30∼40개를 5마리의 비장적출수란가토의 난관에 이식한 다음, 혈소판수의 변화를 조사한 결과, 이식전에 비해 이식후 90분 (P<0.025)과 135∼270분 (P<0.025)에 유의한 감소를 확인하였따. 특히 수정란 이식후 180분경에 가장 크게 감소하였다 (P<0.001). 60∼70개의 2세포기 가토수정란을 24시간 배양한 다음, 배양액을 3마리의 비장적출가토에 주사한 후 혈소판의 변화를 조사하였을 때, 주사후 120분경부터 혈소판의 수가 유의하게 감소하기 시작하였다. 이들 배양액을 동결, 해동한 다음 주사한 실험에서도 유사한 결가를 얻었다. 또한 이 배양액을 비장적출생쥐에 주사한 경우에도 가토에서와 마찬가지의 혈소판 촉진현상이 나타났다. 혈소판 촉진인자(PAF)의 길항제인 kadsurenone이 첨가된 배양액에서 24시간동안 가토수정란을 배양한 다음, 이 배양액을 4마리의 비장적출생쥐에 주사한 결과, 혈소판수의 변화가 일어나지 않았다. 또한 kadsurenone이 첨가된 배양액에서는 2세포기 가토수정란의 상실기와 배반포기까지 발달율은 각각 4와 7%로 대조구의 7과 49%보다 유의하게 낮았다. 따라서 본 연구에서 가토의 초기배는 가토나 생쥐의 혈소판수를 감소시키고, 특히 길항제 처리는 이러한 혈소판 감소현상을 억제시킬 뿐만 아니라 가토 초기배발달을 억제한다는 것을 확인하였다. 결론적으로 가토초기배에서는 PAF 또는 수정란 유래의 PAF가 분비된다는 것을 알 수 있었으며, 이러한 인자는 동결처리에서도 그 기능은 전혀 변하지 않는다고 본다. 이후에 있어서 mouse LIF의 첨가는 돼지의 수정란을 배반포 이후의 단계에까지 발달시킬 수 있었다. 있어서 더 적합한 것으로 판단되었다. 5. 개발된 모형은 논 관개의 물리적 측면과 관리목표 모두를 고려한 것으로 계산된 효율은 벼, 생육 각 단계에서의 효율 비교에 양호한 방법임을 알 수 있다.은 Sharpsburg 점질양토에 대한 S.C.S 한계허용치 10ton/ha/year 이내로 나타났다. 비처리구에서의 토양유실량은 평균 2.56ton/ha/year로 높게 나타난 반면 3개의 서로 다른 추리구인 비수구, 초생수로구 및 Bromegrass구에서는 각각 0.152, 0.192 및 0.290ton/ha/year로 낮은 결과를 가져왔다. 6. 평균 침전량에 대한 L.S.D. 검정 걸과 전시험구중 비처리구가 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low in two aspects, named "the Nonsignificant group". And the issues were high risk perception in general

• The Korean Journal of Mycology
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• v.24 no.2 s.77
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• pp.104-110
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• 1996

The effect of nutritional and cultural conditions for the production of anti-complementary polysaccharide from Pleurotus sajor-caju was studied. Maximum anti-complementary activity from its culture broth and mycelium was obtained by using lactose and maltose as a carbon source, respectively. Ammonium phosphate (monobasic) and serine were chosen for the highest activity among the inorganic nitrogen compounds and amino acids tested. Optimum temperature for the production of the anti-complementary polysaccharide was $25^{\circ}C$.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.181-187
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• 1995

This study was conducted to investigate the effect of NH$_4$H$_2$PO$_4$ on pH of the nutrient solution using municipal tap water during hydroponic culture of crisp lettuce (Lactuca sativa var. capitata) seedlings. The composition of starter solution was different from that of supplementary solution. The pH in the nutrient solution was suddenly declined and recovered as the supplementary solution was supplied. The pH of nutrient solution was increased with high temperature and, on the contrary, the EC of nutrient solution was decreased. It shows that plant absorbed nutrients more than water in given solution when the temperature and light was high. After supplying supplementary solution in 1st and End experiment, pH was slowly increased to 7 in NH$_4$H$_2$PO$_4$ 0.25me/$\ell$, but maintained 6.4-6.5 in NH$_4$H$_2$PO$_4$ 3me/$\ell$ and 6me/$\ell$. In 3rd experiment, pH was slowly increased from 6.7 to 7.4 in NH$_4$H$_2$PO$_4$ 0.25me/$\ell$, but decreased from 6-6.5 to 5-5.5 in NH$_4$H$_2$PO$_4$ 3me/$\ell$ and 6me/$\ell$. So it is suggested that the concentration between 0.25 me/$\ell$ and 3 me/$\ell$ by concentration base or the amount of NH$_4$H$_2$PO$_4$ between 1me/6 $\ell$ and 7me/6 $\ell$ by total quantity in solution is appropriate for stabilizing pH in the nutrient solution. Also this experiment suggests that hand operated measurements must be cautious due to the change of pH and EC within a 24-hour cycle.

• Journal of Embryo Transfer
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• v.18 no.1
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• pp.35-41
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• 2003

Three experiments were performed to develop an optimal culture system of in-vitro derived porcine embryos. In experiment 1, embryos were cultured in two different basic culture media (NCSU-23 and PZH) supplemented with BSA (4mg/ml), FBS (10%), PVA (3mg/ml). Although no difference on the percentage of embryos developed to blastocysts was found (P>0.05), more 2-cell embryos (59.5% vs. 44.7 to 52.0%) were obtained when embryos were cultured in NCSU-23 added with BSA. In experiment 2, identical treatment was conducted using PZM medium. More (P<0.05) 2-cell embryos (54.4% vs. 40.7 to 44.5%) and blastocysts (12.5% vs. 5.8 to 9.0) were produced when embryos were cultured in PZM added with BSA (4mg/ml). In experiment 3, embryos were cultured in media as follows; 1) NCSU-23 without BSA for 192 hours; 2) NCSU-23 with BSA for 192 hours; 3) NCSU-23 with BSA for 96 h and then subsequently injected with FBS into culture drops. Although no difference on the percentage of embryos developed to blastocysts was found (P>0.05), more 2-cell embryos (63.8 to 69.2% vs. 52.0%) were obtained when embryos were cultured both in NCSU-23 added with BSA throughout culture duration and in FBS supplemented medium. In conclusion, no difference was found when porcine preimplantation embryos were cultured in NCSU-23 added with various macromolecules. However, when PZM medium was used, PZM medium supplemented with BSA showed more potential to support embryo development. Finally, FBS supplemented into culture drop 96h post-insemination did not show any benefits on embryo development.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.299-299
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• 1994

펩타이드 약물의 일종인 LHRH 및 그의 analogue인 〔D-Ala$^{6}$)LHRH의 경점막 수송경로를 개발하기 위하여 이들 약물을 점막 균질액과 배양시 점막에 존재하는 효소에 의해 분해되는 것을 발견하였다. 이에 저자등은 LHRH 및 〔D-Ala$^{6}$〕 LHRH의 점막 균질액 중에서의 분해산물을 HPLC에 의해 정량하였다. 또한 PITC법에 따라 아미노산 분석을 실시하여 이들 분해산물의 아미노산 조성을 밝히고 아울러 분해위치를 규명하였다. 즉 LHRH또는 〔D-Ala$^{6}$〕 LHRH를 직장, 비강 및 질점막과 일정시간 배양 후 시료를 HPLC에 주입하여 각 분해산물의 peak time에 용리액을 분획 수집하였다. 이것을 6N-HCl로 가수분해하여 유리아미노산으로 만들고 phenylisothiocyanate 유도체화 하여 PTC 아미노산으로 한후 아미노산 분석용 컬럼을 사용하여 HPLC로 정량 하였다.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.162-169
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• 2008

To determine suitable composition of nutrient solution according to soil textures in fertigation culture of cucumber using three strengths (S) of Yamasaki cucumber recipe, chemical changes of soil, growth characteristics and yield of cucumber were investigated. Electric conductivity of drainage solution was risen in all treatments, pH of loam soil was generally optimum level and that of sandy soil was high level. Photosynthetic rate in loam soil supplied the 1/2 S and transpiration rate in sandy soil supplied the 1.0 S were most low. Diffusive resistance in sandy soil supplied the 1.0 S was high. Chlorophyll contents was higher concentrations by the kinds of soil. Amount of drainage solution in sandy loam soil supplied the 1/2 S and loam soil supplied the 1.0 S were most much and little, respectively. Water absorption rate was the opposition to amount of drainage solution. Nutrient contents in soil except calcium were most high in the 1.0 S by the kinds of soil. Nutrient contents in leaves, nitrogen in sandy soil supplied 1.0 S, phosphorus in loam soil supplied 1/2 S, potassium in two soil supplied 1.0 S, calcium in loam supplied 1/2 S and sandy loam soil supplied 1.0 S, magnesium in loam soil supplied the 1/2 S was high. The growth and yield of fruit were more in loam than in sandy loam soil. Therefore, the suitable compositions of macro-nutrients for fertigation culture of cucumber were determined in loam and sandy loam soils as follows: In loam soil, they were $NO_3$-N 12.3, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 5.7, Mg $3.5\;me{\cdot}L^{-1}$, whereas in sandy loam soil $NO_3$-N 11.7, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 4.9, Mg $3.2\;me\;L^{-1}$.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.269-274
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• 2003

This experiment was carried out to investigate the effects of different IVM-IVC culture media factors, such as development rates according to the maturation media, collecting times from slaughter to initiation of incubation and with cumulus cells, on in vitro maturation of oocytes collected from 3∼5mm diameter follicles of the swine abattoir The development rates significantly(p<0.05) higher when the oocytes were matured TCM-199 media than NCSU-23 media. In comparing with TCM199 medium in presence of Earle's salts and Hank's salt, there were no significantly differences between each salt balance in cleaved rate and in number of morulae plus blastocyst. Among 1,455 immature oocytes, 999(68.6％) of oocytes were cleaved. The number of development to the morulae and blastocysts were 617(61.8％) include 62 balstocysts(6.2％).

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.816-821
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• 2007

This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mulberry leaf extract. The culture broth inhibited melanin synthesis in vitro in the concentration dependent manner of 10 to 50%. The mulberry leaf extract itself has a cytotoxicity to the mouse melanoma cell, B16BL6 with the value of IC$_{50}$ of 7% concentration. But the culture broth of P. japonica in the mulberry leaf extract did not show any cytotoxicity up to 50%. Therefore, we concluded the mycelial culture broth of P. japonica in the extract of mulberry leaf can be used as a whitening cosmetic resource.

• Journal of Bio-Environment Control
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• v.2 no.2
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• pp.147-149
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• 1993

양액재배에 있어서 배양액 중에 있는 질소, 칼륨, 인, 칼슘, 마그네슘 등 식물 영양성분의 함유량을 항상 알맞게 유지시키는 일은 매우 중요하다. 아울러, 양액의 산도(pH)와 용존산소량(dissolved oxygen;DO)도 식물 생장에 큰 영향을 미치기 때문에 적절히 유지하지 않으면 안된다. 양액의 상태를 항상 작물 생육에 알맞게 유시키기 위해서는 먼저 양액의 조성과 관계가 있는 물리량을 정확히 측정할 필요가 있다. 특히 양액관리를 자동화하기 위해서는 정확도와 함께 신속하고도 간편한 측정방법이 필요하다. 양액 상태에 관한 주요 측정항목에는 전기전도도(electric conductivity : EC), 산도(pH), 용존산소량(DO)이 있다.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1993.10a
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• pp.19-20
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• 1993

수경재배에서 이용되고 있는 적절한 질소원은 작물의 종류, 생육단계 등에 따라 차이가 있으므로 들깨의 선호하는 질소형태를 구명하므로서 수경재배를 이용하여 들깨잎을 생산하는데 적절한 배양액 조성을 연구하기 위하여 행해졌다. (중략)