Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of In-Vitro Fertilization of Porcine Matured Oocytes in Different IVM-IVC Culture Media on the Development of the Embryos

미성숙 돼지 난자가 다른 체외성숙.배양에 의한 배 발달률에 미치는 영향

  • 안미현 (단국대학교 생명 자원과학대학 동물자원과학과) ;
  • 홍대욱 (단국대학교 생명 자원과학대학 동물자원과학과) ;
  • 석호봉 (단국대학교 생명 자원과학대학 동물자원과학과)
  • Published : 2003.12.01
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Abstract

This experiment was carried out to investigate the effects of different IVM-IVC culture media factors, such as development rates according to the maturation media, collecting times from slaughter to initiation of incubation and with cumulus cells, on in vitro maturation of oocytes collected from 3∼5mm diameter follicles of the swine abattoir The development rates significantly(p<0.05) higher when the oocytes were matured TCM-199 media than NCSU-23 media. In comparing with TCM199 medium in presence of Earle's salts and Hank's salt, there were no significantly differences between each salt balance in cleaved rate and in number of morulae plus blastocyst. Among 1,455 immature oocytes, 999(68.6％) of oocytes were cleaved. The number of development to the morulae and blastocysts were 617(61.8％) include 62 balstocysts(6.2％).

본 연구는 미성숙 돼지난자가 체외성숙$.$배양액인 TCM-199과 NCSU-23배지에서의 배양상태를 비교하였고, 체외성숙용 TCM-199배지에 Earle'ssalt와 Hank's salt의 첨가에 의한 세포분화와 배발달 상태를 각각 조사하였다. 1. TCM-199와 NCSU-23 배양액에 각각의 supplement를 첨가하여 5% $CO_2$조건하에서 체외성숙을 유기한 결과, TCM-199 배양액에서 유의성(p<0.05)이 검증되었다. 2. 체외성숙용 TCM-199배지의 Earle's salt와 Hank's salt의 조성 성분에 따른 미성숙 돼지난포란의 배발달률은 Earle's가 Hank's보다 약간 높았으나 유의적인 차이는 나타나지 않았다. 3. 총1,455개의 미성숙 난포란 중에서 999(68.6%)개가 세포분화를 나타내었고, 그중 blastocyst의 62개(6.2%)를 포함한 617개(61.8%)의 mo-rulae와 blastocyst의 배발달을 보였다.

Keywords

References

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