Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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nhibition of Melanin Synthesis by Mycelial Culture Broth of Paecilomyces japonica in the Mulberry

뽕잎 추출물을 이용한 동충하초(Paecilomyces japonica) 균사체 배양액의 멜라닌 합성 억제 효과

  • Park, Sang-Sang (Department of Microbiological Engineering, JinJu National University) ;
  • Ryu, Young-Bae (Department fo Applied Life Science, Gyeongsang National University) ;
  • Lee, Young-Hoon (Department of Microbiological Engineering, JinJu National University) ;
  • Cho, Yong-Un (Department of Microbiological Engineering, JinJu National University) ;
  • Cho, Soo-Jeong (Department of Microbiological Engineering, JinJu National University) ;
  • Choi, Young-Ju (Department of Food Nutrition, Silla University Busan) ;
  • Park, Ki-Hoon (Department fo Applied Life Science, Gyeongsang National University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, JinJu National University)
  • 박상상 (진주산업대학교 미생물공학과) ;
  • 류영배 (경상대학교 응용생명과학부) ;
  • 이영훈 (진주산업대학교 미생물공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 조수정 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 박기훈 (경상대학교 응용생명과학부) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Published : 2007.06.25
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Abstract

This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mulberry leaf extract. The culture broth inhibited melanin synthesis in vitro in the concentration dependent manner of 10 to 50%. The mulberry leaf extract itself has a cytotoxicity to the mouse melanoma cell, B16BL6 with the value of IC$_{50}$ of 7% concentration. But the culture broth of P. japonica in the mulberry leaf extract did not show any cytotoxicity up to 50%. Therefore, we concluded the mycelial culture broth of P. japonica in the extract of mulberry leaf can be used as a whitening cosmetic resource.

본 연구는 뽕잎 추출물을 이용한 동충하초 균사체 배양액의 in vivo, in vitro멜라닌 생성 억제 효과를 조사한 것이다. 뽕잎 추출물을 이용한 동충하초 균사체 배양액은 10~50%의 농도 의존적으로 시험관 내에서 멜라닌 합성관련 효소활성의 저해를 나타냈다. 뽕잎 추출물 자체는 mouse melanoma cell (B16BL6)에 대한 세포독성을 보였으며 IC$_{50}$%값이 약 7% 농도로 나타난 반면, 뽕잎 추출물을 이용한 동충하초 균사체배양액은 50% 농도에서도 어떠한 세포독성도 관찰되지 않음을 확인할 수 있었다. 또한 B16BL6 mouse melanoma cell의 세포 내 멜라닌 생합성 억제에 대해서도 높은 활성을 나타내었다. 따라서, 뽕잎 추출물을 이용한 동충하초 균사체 배양액은 세포에 대한 독성이 전혀 없으며, 멜라닌 생합성 저해 작용이 우수하여 피부 미백용 화장료 조성물로서의 이용 가능성이 확인 되었다.

Keywords

References

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