• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1994.11a
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• pp.74-75
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• 1994

양액재배에서 고려해야할 문제는 근권의 환경관리인데, 그 중에서도 가장 중요한 문제는 배양액의 관리이다. 배양액의 관리는 재배환경 및 작물의 생육단계에 따라서 달리해야 하는데, 현재 농가에서의 배양액관리는 재배경험이 일천한데다 사용하는 배양액에 따른 생장과 발육반응에 대한 결과가 미흡하다. 이런 측면에서 토마토의 분무경재배시 일반적으로 가장 많이 사용하는 배양액조성들이 방울토마토의 생장과 과실생장에 미치는 작용을 살펴보고, 방울토마토 양액재배시 성공적인 배양액관리를 위한 기초자료로 활용하고자 본 실험을 수행하였다. (중략)

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1992.12a
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• pp.12-13
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• 1992

양액재배에 따른 배양액 관리의 자동화가 진행될수록, 배양액을 효율적으로 관리할 수 있는 배지원시스템이 필요하다. 최근 시설원예의 환경관리 등에 AI 수법이 도입되고 있고 양액관리도 예외는 아니다. 신경회로망은 패턴인식, 학습 등의 분야에서 유망한 수법으로 인정되고 있고 통계적수법에 유사한 분석 등에도 적용되고 있다. 본 연구에서는 신경회로망을 이용하여 각 이온의 농도를 입력(독립변수), 전기전도도를 출력(종속변수)로 하는 비선형 증회귀분석을 시행하여 배양액의 전기전도도의 추정 및 신경회로망의 적용성 여부를 검토하였다. (중략)

• Journal of Bio-Environment Control
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• v.1 no.1
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• pp.52-60
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• 1992

The optimum management of nutrient solution needs the effective combination of fertilizers as well as the accurate control of nutrient solution. This study was attempt to make a supporting system for effective fertilizer combination by using computer and also to develop a EC predicting equation for keeping the EC of solution within the allowable range after application of combined fertilizers. The supporting system consists of three parts : (1) data bases, (2) rules for deciding the kinds and amounts of fertilizers and (3) main control. With input data, the main control automatically constructs the network connecting the related data bases and subsequently executes the operation of searching proper fertilizers through it. For more effective searching, fertilizers are classified into two levels(level 1 and level 2) in consideration of solubility, price, and frequency in use, and searched in that order. The EC prediction equation, a extended form of the Robinson and Stroke's theoretical equation only available for a binary electrolyte, is suggested for predicting the EC of the nutrient solution containing many kinds of inorganic compounds. The comparison of predicted and measured ECs showed good agreements with the high correlation between the predicted EC decrement by ion interaction and the actual one(limiting EC minus measured EC).

• Journal of Korean Ophthalmic Optics Society
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• v.1 no.2
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• pp.13-18
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• 1996

In this study, contact lens care solutions were compared to each other for cytotoxic effect on cultured mouse fibroblasts by the use of neutral red(NR) assay. This study tested the cytotoxicity of 12 cleaning solutions, 2 lubricants and 5 multipurpose solutions(MPS) at 1%, 2%, 3% and 5% concentrations. These solutions are manufactured in Korea and foreign countries. The relative cytotoxic comparison of these solutions showed that some of them are toxic, three of the cleaning solutions were especially highly toxic 10 cells, so most fibroblasts were dead at 1% concentration. The toxic cleaning solutions have more components than other solutions. But both lubricants and MPSs are non-cytotoxic 10 cells. Some of these solutions did not have any descriptions or indications. They are very dangerous to eyes. From this study, contact lens care solutions should be tested for cytotoxic effects.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.812-818
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• 2014

Raising seedlings is important for fruit crops and is especially significant for strawberries as it accounts for 80% of their cultivation. However, there are few studies on raising seedlings of strawberries by hydroponics. Since strawberries show clear differences in growth characteristics based on cultivar, it is necessary to develop suitable fertilizer formula, concentration and pH for each cultivar, and also to examine the amount of nutrient feeding appropriate for each medium type. A key to raising seedlings of strawberries by hydroponics is the development of strategies to manage the concentration of nutrient solution. The mother plants of 'Daewang' strawberries were planted on hydroponics benches filled with cocopeat on March 28, 2012. Three nutrient solution treatments were employed during the term of raising seedlings: a type that supplied EC $0.6dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [0.6 (30) + 20]; a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [1.2 (30) + 20]; and a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 50 days [1.2 (50)]. The plants were then planted on hydroponics benches filled with cocopeat on September 20, and managed with EC $0.6-0.8dS{\cdot}m^{-1}$ strawberry nutrient solution developed by Yamazaki. After planting, shoot growth, flowering rate and fruit quality of the first cluster were investigated. The petiole length, leaf length, leaf width and crown diameter showed the highest grown in the [1.2 (50)] treatment, followed by [1.2 (30) + 20], and then [0.6 (30) + 20], indicating that the higher concentration of nutrient solution was preferable for raising seedlings. However, the growth differences among treatments gradually disappeared as growth continued, and the crown diameter especially grew to exhibit almost no difference at all among treatments. The point of flowering came first in [0.6 (30) + 20], followed by [1.2 (30) + 20] and then [1.2 (50)]. The [0.6 (30) + 20] treatment showed much earlier flowering than other treatments, which implies that low-concentration nutrient solution may be beneficial to the flowering of 'Daewang' strawberries while raising seedlings. There was no statistically significant difference among treatments in fruit length, fruit diameter and fruit firmness. Fruit weight in [1.2 (50)] treatment was significantly higher than other treatments. However, soluble solids of fruit was the lowest in [1.2 (50)] treatment. Together, the results of this experiment imply that it is better to supply EC $0.6dS{\cdot}m^{-1}$ solution for 30 days and then supply only water for 20 days to adequately manage concentration of nutrient solutions during the period of raising seedlings of strawberries by hydroponics.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.163-169
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• 2001

OSBA(oocytes-sperm binding assay) is a tool developed for rapid test of optimal condition of IVF medium and protein source by binding ability of mouse sperm and egg. Mouse oocyte-cumulus complexes were prepared by removing of the cumulus cells with 0.1% hyaluronidase. 10$\pm$2 oocytes per 30 ${mu}ell$ medium drop were inseminated with 3 ${mu}ell$ sperm suspension and were cultured f3r 3 hours and 24 hours, respectively. And the oocytes were recovered gently and the No. of sperm bound on oocytes were counted. In the Exp. 1, the ratio of oocytes bound with one sperm at least were 60.2%(50/83), 2%(2/77) and 100%(79/79) in the medium with no protein, FBS(15%, v/v) and BSA(0.4%. w/v), respectively, Fetal bovine serum(FBS) seriously inhibited sperm binding on oocyte, although bovine serum albumin(BSA) promoted the binding ability. The inhibiting effect of FBS was dependent on the concentration of FBS. The sperm binding ability according to oocyte maturity was tested in the Exp. 2. There was no significant difference between Met. II (mature) and Met. I (intermediate mature) oocytes in the number of oocytes bound with sperm and the number of sperm bound on oocytes. Finally, in Exp. 3, two batches of Ham's F10 medium with good and poor quality by OSBA were tested (The ratios of embryos developed from PN 1-cell stage to hatched blastocyst; 25% vs. 70%). In the medium with good quality, sperm binding ability was significantly increased (P ＜ 0.05). The ratio of oocytes bound with one sperm at least was 66% and 90% in the medium with poor and good quality, respectively. Conclusively, It was possible to test IVF medium condition rapidly and easily by OSBA.

• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.251-259
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• 2018

BACKGROUND: Various culture media have been used for hydroponic cultures of horticultural plants under the smart greenhouses with natural and artificial light types. Management of the culture medium for the control of medium amounts and/or necessary components absorbed by plants during the cultivation period is performed with ICT (Information and Communication Technology) and/or IoT (Internet of Things) in a smart farm system. This study was conducted to develop the cloud-based data analysis system for effective management of culture medium applying to hydroponic culture and plant growth in smart greenhouses. METHODS AND RESULTS: Conventional inorganic Yamazaki and organic media derived from agricultural byproducts such as a immature fruit, leaf, or stem were used for hydroponic culture media. Component changes of the solutions according to the growth stage were monitored and plant growth was observed. Red and green lettuce seedlings (Lactuca sativa L.) which developed 2~3 true leaves were considered as plant materials. The seedlings were hydroponically grown in the smart greenhouse with fluorescent and light-emitting diodes (LEDs) lights of $150{\mu}mol/m^2/s$ light intensity for 35 days. Growth data of the seedlings were classified and stored to develop the relational database in the virtual machine which was generated from an open stack cloud system on the base of growth parameter. Relation of the plant growth and nutrient absorption pattern of 9 inorganic components inside the media during the cultivation period was investigated. The stored data associated with component changes and growth parameters were visualized on the web through the web framework and Node JS. CONCLUSION: Time-series changes of inorganic components in the culture media were observed. The increases of the unfolded leaves or fresh weight of the seedlings were mainly dependent on the macroelements such as a $NO_3-N$, and affected by the different inorganic and organic media. Though the data analysis system was developed, actual measurement data were offered by using the user smart device, and analysis and comparison of the data were visualized graphically in time series based on the cloud database. Agricultural management in data visualization and/or plant growth can be implemented by the data analysis system under whole agricultural sites regardless of various culture environmental changes.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.252-260
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• 2008

This study was conducted to observe the characteristics of mineral nutrient uptake of single-node cutting rose 'Versilla' and to determine optimal nutrient solution control method for soilless culture of 'Versilla' in a closed hydroponic system. Nutrient solution was managed by five different control methods: macro- and micro-element control in aeroponic system (M&M), macroelement control in aeroponic system (M), nutrient solution supplement in aeroponic system (S); electrical conductivity (EC) control in aeroponic system(EC-A); EC control in deep flow technique system(EC-D). The concentration of $NO_3$-N exceeds optimal range whereas P and Mg decreased at the later stage of plant growth with the EC control method, EC-A and EC-D. The overall mineral nutrient content increased with S. On the other hand the nutrient content at the root environment was maintained optimal with M&M and M. The nutrient solution control methods had significantly effect on the cut-flower quality. In the M&M and M, flower length, fresh weight and root activity were higher than those with the other mineral nutrients control methods. The maximal efficiency of photochemistry (Fv/Fm) was higher for M&M, M and S than that with EC-A and EC-D. Based on the above results, it is highly recommended to control nutrient solution by mineral nutrient control methods (M&M and M) in a closed hydroponic system for single-node cutting rose, 'Versillia'.

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.149-154
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• 2005

It is very important to make shorter and healthier pot plants with increased numbers of branch at a growing stage before short-day exposure. Especially light and nutrient conditions directly affect the growth and quality of the plants as described above. In this study, the effects of nutrient strength and light intensity on the nutrient uptake and growth of young Kalanchoe plants (Kalanchoe blossfeldiana 'Marlene') during this growth stage were investigated. The plants were grown under two radiation integral (15.8 and 7.9 $mol{\cdot}m^{-2}{\cdot}d^{-1}$, PPF) and three EC (0.8, 1.6 and 2.4 $dS{\cdot}m^{-1}$) conditions. Leaf area, fresh weight, dry weight and number of branch were higher at a higher PPF, and this tendency was more evident at an EC above 1.6$dS{\cdot}m^{-1}$. The plants became higher at a lower PPF. When the EC was at 0.8 $dS{\cdot}m^{-1}$, the plants did not grow so healthy regardless of PPF conditions. EC decrement in the nutrient solution was increased with increase of nutrient strength. With growth stage, the nutrient uptake was increased with increases of nutrient strength and PPF. At a higher PPF, $NO_3-N,\;K^{+}\;and\;Ca^{2+}$ were much more absorbed, and especially the uptake of $K^{+}$ was 1.1 to 1.5 times greater than that or $NO_3-N$. From the results, the EC needed above 1.6 $dS{\cdot}m^{-1}$ during the seedling stage in order to make more healthy Kalanchoe plants having more leaf area, fresh weight, dry weight and number of branches under adequate light conditions.

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.258-265
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• 2004

The experiment was conducted to investigate the effect of EC and pH of nutrient solution on the growth and quality of the single-stemmed rose grown in a rose factory installed with hydroponic system. The growth and quality of the single-stemmed rose were not significantly different from each other with the EC of the nutrient solutions $1.0\~3.5dS{\cdot}m^{-1}$, which resulted in concluding high concentration of the nutrient solution was not necessary. The optimum range of the EC for single-stemmed rose was $1.5\~2.0dS{\cdot}m^{-1}$ considering plant growth, photosynthetic and transpiration rates. The optimum range of the pH for good plant growth without any visible physiological disorder was $4.0\~6.0$. Therefore, to keep the pH of the nutrient solution for rose low compared to other plants was beneficial for plant growth and uptake of the mineral ions.