• Journal of Embryo Transfer
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• 제26권1호
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• pp.19-25
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• 2011

This study was carried out to evaluate the effect of early pregnant cow as donor for Ovum Pick-Up (OPU) derived oocyte aspiration and embryo production in Holstein heifers. Four non-pregnant and 2 pregnant Holstein heifers were used as donor and then carried out total 17 OPU session for 10 weeks (2 times per week). Recovered cumulus-oocyte-complexes (COCs) were classified into 4 grade by oocyte cytoplasm and cumulus cells and matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml FSH and 1 mg/ml estradiol in 5% $CO_2$ and over 99% humidity for 24 h. After 24 h co-incubation with post-thaw sperm, the presumed zygotes were cultured in CR1aa medium with 4 mg/ml BSA for 3 days and then changed CR1aa medium with 10% of FBS for another 3~4 days. The Mean number of aspirated follicles and collected oocytes in the early stage pregnant and non-pregnant heifers were $13.0{\pm}4.3$ and $10.6{\pm}3.9$, $5.4{\pm}3.4$ and $7.7{\pm}3.6$ per session, respectively. Rate of collected oocyte from aspirated follicles were 59.2% and 50.5%, respectively. The average number of good quality oocytes (Grade I and II) in the early stage pregnant and non-pregnant heifers was $3.7{\pm}2.7$ and $4.9{\pm}2.6$ (Mean${\pm}$SD). Cleavage and blastocyst developmental rates in Grade I and II were 22.2% and 25.5%, and then $1.7{\pm}0.9$ and $1.4{\pm}1.1$ blastocyst per session, respectively. In conclusion, OPU technology can be used in early stage pregnant and non-pregnant heifers without any problem and so applied OPU derived embryo production to maximize the ability of genetically valuable females.

• Korean Journal of Agricultural and Forest Meteorology
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• 제19권3호
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• pp.75-85
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• 2017

A method to estimate hourly temperature profiles on calm and clear nights was developed based on temporal changes of inversion height and strength. A meteorological temperature profiler (Model MTP5H, Kipp and Zonen) was installed on the rooftop of the Highland Agriculture Research Institute, located in Daegwallyeong-myeon, Pyeongchang-gun, Gangwon-do. The hourly vertical distribution of air temperature was measured up to 600 m at intervals of 50 m from May 2007 to March 2008. Temperature and relative humidity data loggers (HOBO U23 Pro v2, Onset Computer Corporation, USA) were installed in the Jungdae-ri Valley, located between Gurye-gun, Jeollanam-do and Gwangyang-si, Jeollanam-do. These loggers were used to archive measurements of weather data 1.5 m above the surface from October 3, 2014, to November 23, 2015. The inversion strength was determined using the difference between the temperature at the inversion height, which is the highest temperature in the profile, and the temperature at 100 m from the surface. Empirical equations for the changes of inversion height and strength were derived to express the development of temperature inversion on calm and clear nights. To estimate air temperature near the ground on a slope exposed to crops, the equation's parameters were modified using temperature distribution of the mountain slope obtained from the data loggers. Estimated hourly temperatures using the method were compared with observed temperatures at 19 weather sites located within three watersheds in the southern Jiri-mountain in 2015. The mean error (ME) and root mean square error (RMSE) of the hourly temperatures were $-0.69^{\circ}C$ and $1.61^{\circ}C$, respectively. Hourly temperatures were often underestimated from 2000 to 0100 LST the next day. When temperatures were estimated at 0600 LST using the existing model, ME and RMSE were $-0.86^{\circ}C$ and $1.72^{\circ}C$, respectively. The method proposed in this study resulted in a smaller error, e.g., ME of $-0.12^{\circ}C$ and RMSE of $1.34^{\circ}C$. The method could be improved further taking into account various weather conditions, which could reduce the estimation error.

• Ecology and Resilient Infrastructure
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• 제8권4호
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• pp.223-232
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• 2021

In general, stormwater flows to the road surface, especially in urban areas, and it is discharged through the drainage grate inlets on roads. The appropriate evaluation of the road drainage capacity is essential not only in the design of roads and inlets but also in the design of sewer systems. However, the method of road surface flow analysis that reflects the topographical and hydraulic conditions might not be fully developed. Therefore, the enhanced method of road surface flow analysis should be presented by investigating the existing analysis method such as the flow analysis module (uniform; varied) and the flow travel time (critical; fixed). In this study, the algorithm based on varied and uniform flow analysis was developed to analyze the flow pattern of road surface. The numerical analysis applied the uniform and varied flow analysis module and travel time as parameters were conducted to estimate the characteristics of rainfall-runoff in various road conditions using the developed algorithm. The width of the road (two-lane (6 m)) and the slope of the road (longitudinal slope of road 1 - 10%, transverse slope of road 2%, and transverse slope of gutter 2 - 10%) was considered. In addition, the flow of the road surface is collected from the gutter along the road slope and drained through the gutter in the downstream part, and the width of the gutter was selected to be 0.5 m. The simulation results were revealed that the runoff characteristics were affected by the road slope conditions, and it was found that the varied flow analysis module adequately reflected the gutter flow which is changed along the downstream caused by collecting of road surface flow at the gutter. The varied flow analysis module simulated 11.80% longer flow travel time on average (max. 23.66%) and 4.73% larger total road surface discharge on average (max. 9.50%) than the uniform flow analysis module. In order to accurately estimate the amount of runoff from the road, it was appropriate to perform flow analysis by applying the critical duration and the varied flow analysis module. The developed algorithm was expected to be able to be used in the design of road drainage because it was accurately simulated the runoff characteristics on the road surface.

• Journal of Climate Change Research
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• 제5권1호
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• pp.13-19
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• 2014

In the past, Korea agro-climatic zone except Jeju-do was classified into nineteen based on rice culture by using air temperature, precipitation, and sunshine duration etc. during rice growing periods. It has been used for selecting safety zone of rice cultivation and countermeasures to meteorological disasters. In this study, the climatic variables such as air temperature, precipitation, and sunshine duration of twenty agro-climatic zones including Jeju-do were compared decennially (1970's, 1980's, 1990's, and 2000's). The meteorological data were obtained in Meteorological Information Portal Service System-Disaster Prevention, Korea Meteorological Administration. The temperature of 1970s, 1980s, 1990s, and 2000s were $12.0{\pm}0.14^{\circ}C$, $11.9{\pm}0.13^{\circ}C$, $12.2{\pm}0.14^{\circ}C$, and $12.6{\pm}0.13^{\circ}C$, respectively. The precipitation of 1970s, 1980s, 1990s, and 2000s were $1,270.3{\pm}20.05mm$, $1,343.0{\pm}26.01mm$, $1,350.6{\pm}27.13mm$, and $1,416.8{\pm}24.87mm$, respectively. And the sunshine duration of 1970s, 1980s, 1990s, and 2000s were $421.7{\pm}18.37hours$, $2,352.4{\pm}15.01hours$, $2,196.3{\pm}12.32hours$, and $2,146.8{\pm}15.37hours$, respectively. The temperature in Middle-Inland zone ($+1.2^{\circ}C$) and Eastern-Southern zone ($+1.1^{\circ}C$) remarkably increased. The temperature increased most in Taebak highly Cold zone ($+364mm$) and Taebak moderately Cold Zone ($+326mm$). The sunshine duration decreased most in Middle-Inland Zone (-995 hours). The temperature (F=2.708, df=3, p= 0.046) and precipitation (F=5.037, df=3, p=0.002) increased significantly among seasons while the sunshine duration decreased significantly(F=26.181, df=3, p<0.0001) among seasons. In further study, it will need to reclassify agro-climatic zone of rice and it will need to conduct studies on safe cropping season, growth and developing of rice, and cultivation management system etc. based on reclassified agro-climatic zone.

• Korean Journal of Heritage: History & Science
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• 제55권2호
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• pp.102-111
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• 2022

Termites are a group of social insects that are one of the primary causes of damage to wooden architectural heritage. Since termite damage impairs the authenticity and structural stability of cultural heritage, it is imperative to prevent it. This study examines the extent of termite damage to wooden architectural heritage as part of efforts to prevent termite damage to nationally designated wooden architectural heritage sites across the country. The extent of termite damage to each cultural heritage was assessed qualitatively and quantitatively and comparatively analyzed by region using the results of the "Investigation on Biological Damage to Wooden Architectural Heritages" conducted by the National Research Institute of Cultural Heritage from 2016 to 2019. It involved 362 nationally designated wooden architectural heritages(25 national treasures, 157 treasures, 180 national folklore cultural heritages) and 1,104 buildings. The results were as follows: termite detection dogs reacted at 317(87.6%) of the 362 wooden heritages, with visible termite damage observed in 185 cases(51.1%). Furthermore, termite damage was confirmed using one of two methods(detection dogs or visual inspection) in 324 cases(89.5%). Of the 1,104 buildings, termite detection dogs reacted at 668(60.5%), while 339(30.7%) showed visible termite damage. Employing one of the two methods, damage was confirmed in 702 buildings(63.6%). The country was categorized into nine regions(Seoul Metropolitan Area, Gangwon, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Gyeongbuk, Gyeongnam, and Jeju) to examine the termite damage rate and the degree of damage to each cultural heritage according to location. Termite detection dogs reacted to more than 70% of the cultural heritage in all regions. Visible damage was minimal in the Seoul metropolitan area(32.1%) and Gangwon(21.4%) but severe in Chungnam(65.6%), Jeonnam(67.3%), and Gyeongnam(68.2%). By quantifying the degree of termite damage of each cultural heritage as a ratio of the absence of termite damage among the total absence, the average termite damage of the cultural heritage across the country was 9.2%. Regional variance analysis showed that the cultural heritage in Jeonbuk and Jeonnam showed a statistically significantly higher degree of termite damage than the cultural heritage in the Seoul metropolitan area, Chungbuk, and Gyeongbuk. This paper comprehensively analyzed termite damage to nationally designated wooden architectural heritage. The findings are expected to be valuable in establishing policies for the preservation and management of cultural heritage sites in the future.

• Journal of Korea Water Resources Association
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• 제56권11호
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• pp.775-784
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• 2023

With the increasing trend of extreme rainfall that exceeds the design frequency of man-made structures due to extreme weather, it is necessary to review the safety of agricultural reservoirs designed in the past. However, there are no local government-managed reservoirs (13,685) that can be discharged in an emergency, except for reservoirs over a certain size under the jurisdiction of the Korea Rural Affairs Corporation. In this case, it is important to quickly deploy a mobile siphon to the site for preliminary discharge, and this study evaluated the applicability of a mobile siphon with a diameter of 200 mm, a minimum water level difference of 6 m, 420 (m²/h), and 10,000 (m²/day), which can perform both preliminary and emergency discharge functions, to the Yugum Reservoir in Gyeongju City. The test bed, Yugum Reservoir, is a facility that was completed in 1945 and has been in use for about 78 years. According to the hydrological stability analysis, the lowest height of the current dam crest section is 27.15 (EL.m), which is 0.29m lower than the reviewed flood level of 27.44 (EL.m), indicating that there is a possibility of lunar flow through the embankment, and the headroom is insufficient by 1.72 m, so it was reviewed as not securing hydrological safety. The water level-volume curve was arbitrarily derived because it was difficult to clearly establish the water level-flow relationship curve of the reservoir since the water level-flow measurement was not carried out regularly, and based on the derived curve, the algorithm for operating small and medium-sized old reservoirs was developed to consider the pre-discharge time, the amount of spillway discharge, and to predict the reservoir lunar flow time according to the flood volume by frequency, thereby securing evacuation time in advance and reducing the risk of collapse. Based on one row of 200 mm diameter mobile siphons, the optimal pre-discharge time to secure evacuation time (about 1 hour) while maintaining 80% of the upper limit water level (about 30,000 m²) during a 30-year flood was analyzed to be 12 hours earlier. If the pre-discharge technology utilizing siphons for small and medium-sized old reservoirs and the algorithm for reservoir operation are implemented in advance in case of abnormal weather and the decision-making of managers is supported, it is possible to secure the safety of residents in the risk area of reservoir collapse, resolve the anxiety of residents through the establishment of a support system for evacuating residents, and reduce risk factors by providing risk avoidance measures in the event of a reservoir risk situation.

• Journal of Korea Water Resources Association
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• 제56권11호
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• pp.751-764
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• 2023

Gangwon State is centered on the Taebaek Mountains with very different climate characteristics depending on the region, and localized heavy rainfall is a frequent occurrence. Heavy rain disasters have a short duration and high spatial and temporal variability, causing many casualties and property damage. In the last 10 years (2012~2021), the number of heavy rain disasters in Gangwon State was 28, with an average cost of 45.6 billion won. To reduce heavy rain disasters, it is necessary to establish a disaster management plan at the local level. In particular, the current criteria for heavy rain warnings are uniform and do not consider local characteristics. Therefore, this study aims to propose a heavy rainfall warning criteria that considers the threshold rainfall for the advisory areas located in Gangwon State. As a result of analyzing the representative value of threshold rainfall by advisory area, the Mean value was similar to the criteria for issuing a heavy rain warning, and it was selected as the criteria for a heavy rain warning in this study. The rainfall events of Typhoon Mitag in 2019, Typhoons Maysak and Haishen in 2020, and Typhoon Khanun in 2023 were applied as rainfall events to review the criteria for heavy rainfall warnings, as a result of Hit Rate accuracy verification, this study reflects the actual warning well with 72% in Gangneung Plain and 98% in Wonju. The criteria for heavy rain warnings in this study are the same as the crisis warning stages (Attention, Caution, Alert, and Danger), which are considered to be possible for preemptive rain disaster response. The results of this study are expected to complement the uniform decision-making system for responding to heavy rain disasters in the future and can be used as a basis for heavy rain warnings that consider disaster risk by region.

• Journal of Korea Water Resources Association
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• 제57권6호
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• pp.421-435
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• 2024

In April 2023, a wildfire broke out in Gangneung located in the east coast region due to the influence of the Yanggang-local wind. In this study, GIS-based RUSLE(Revised Universal Soil Loss Equation) and SEMMA (Soil Erosion Model for Mountain Areas) were used to evaluate the erosion rate due to vegetation recovery in a small watershed of the Gangneung WUI(Wildland-Urban Interface) fire. The small watershed of WUI fire has a low altitude range of 10-30 m and the average slope of 10.0±7.4° which corresponds to a gentle slope. The soil texture was loamy sand with a high organic content and the deep soil depth. As herbaceous layer regenerated profusely in the gully after the wildfire, the NDVI (Normalized Difference Vegetation Index) reached a maximum of 0.55. Simulation results of erosion rates showed that RUSLE ranged from 0.07-94.9 t/ha/storm and SEMMA ranged from 0.24-83.6 t/ha/storm. RUSLE overestimated the average erosion rate by 1.19-1.48 times compared to SEMMA. The erosion rates were estimated to be high in the middle slope where burned pine trees were widely distributed and the slope was steep and to be relatively low in the hollow below the gully where herbaceous layer recovers rapidly. SEMMA showed a rapid increase in erosion sensitivity under at certain vegetation covers with NDVI below 0.25 (Ic = 0.35) on post-fire hillslopes. Gentle slopes with high organic content and rapid recovery of natural vegetation had relatively low erosion rate compared to steep slopes. As subsequent infrastructure and human damages due to sediment disaster by heavy rain is anticipated in WUI fire areas, the research results may be used as basic data for targeted management and decision making on the implementation of emergency treatment after the wildfire.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• 제11권4호
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• pp.158-164
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• 2006

Yongdam Lake is the fifth largest artificial lake in Korea newly formed by the first impounding the Yongdam Multi-purpose Dam on December, 2002. Yongdam Lake, with her total water storage of 820 million M/T, is located at the roof-top region of the streams flowing into the just-constructed new Saemankeum Lake. Seasonal succession of phytoplakton in Yongdam Lake might affect cyanobacterial blooms in Saemankeum Lake by inoculating seasonal dominants. During 2002-2003 when the first impounding after the construction of Yongdam Multi-purpose Dam was still undergoing, summer cyanobacterial blooms by Anabaena, Microcystis, and Aphanizomenon were observed. Among these three, filamentous Anabaena is well known to have its species with $N_2-fixing$ ability and special cells such as heterocysts and akinetes as well as the vegetative cells. We established a clonal culture of Anabaena spiroides v. crasse (KNU-YD0310) from the live water samples collected at the bloom site of Yongdam Lake. The N- and P-nutrient requirement of the KNU-YD0310 was explored by the experimental cultivation of the laboratory strain. Ratio of heterocysts to vegetative cells increased as N-deficiency extended with its maximum at $N_2-fixing$ condition. The strain KNU-YD0310 exhibited considerable growth under N-limiting conditions while its growth was proportional to the initial phosphate-P concentration under P-deficient conditions. Under P-limiting conditions akinete density increased, which could be interpreted as an adaptation strategy to survive severe environment by transforming into resting stage. The above eco-physiological characteristics of Anabaena spiroides v. crassa might be useful as an ecological criterion in controlling cyanobacterial blooms at Shaemankeum Lake in near future.