• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.199-200
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• 2001

해조류중 특히 방사무늬 김 (Porphyra yezoensis)의 양식기술은 인공채묘기술이 개발된 이래 양식 및 가공분야의 많은 기술적 진보를 거듭해 오고있으며 양식장은 천해 해역으로 하천수의 유입으로 인한 풍부한 영양염류의 공급을 받고있는 지역에 있다. 이러한 영양염류는 김의 생장촉진에 도움을 주는 반면에 여러 가지 미생물, 미세조류, 부착조류 등의 증식도 함께 촉진하여 결과적으로 김 성장에 대한 영양분의 경쟁관계, 질병유발 김 양식의 생산력 저하등의 문제점들을 동시에 유발하고 있다. (중략)

• KSBB Journal
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• v.14 no.6
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• pp.702-706
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• 1999

The genetic responses of aquaculturable seaweed Prophyra yezoensis tissue by acid shock have been compared using differential display technique. The tissue has been challenged in seawater containing 0.05% hydrogen chloride(pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs, respectively. Total RNA was extracted by LiCl-guanidinium method. The cDNA was synthesized by reverse transcription with random hexamers and amplified by PCR with arbitrary primers. The genetic fragment disappeared by acid shock was selectively isolated from agarose gel and sequenced with a DNA auto sequencer. One of the acid-labile gene(605 bp) was identified as a dethiobiotin synthetase gene according to sequence alignment analysis by the NCBI BLAST search program.

• Journal of Life Science
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• v.9 no.1
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• pp.15-21
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• 1999

The random amplified ploymorphic DNAs (RAPD) assay is a simple and useful tool in identification of appropriate genetic markers, that requires no knowledge of target DNA sequence. RAPD products were generated directly from seaweed tissues, without prior nucleic acid extraction, of Porphyra yezoensis, Ulva pertusa and Undaria pinnatifida. The nuclear rDNA internal transcribed spacer (ITS) fragment however was not amplified directly from the seaweed tissues. Using DNA extracted by the LiCl method, both the ITS and RAPD's have been amplified by the polymerase chain reaction. RAPD of P yezoensis, thallus (n) and conchocelis (2n) produced lots of different polymorphic bands (36-50$\%$) depending on the arbitrary primers used. Difference was also observed between direct tissues amplification and DNA extracts amplification (53-57$\%$). Thus it is important to use the same ploidy of tissue for DNA extraction and as a RAPD template.

• Journal of Aquaculture
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• v.2 no.1
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• pp.1-7
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• 1989

Axenic tissue culture of a marine red algae Porphyra yezoensis foma narawaensis was established for the vegetative propagation of tissues as a seed stock and for the development of a low salinity-tolerant cell line. Callus tissues have been induced from the vegetative area of blade away from the hold fast when grown on PES-agar medium. The brownish red fragile callus was maintained under fluorescent light of ca. 2000 lux with 12 : 12 hr L : D at $16^{\circ}C$. Amounts of carbohydrate and protein was determined against the weight of callus. Optimum temperature of the callus growth was $14^{\circ}C\~18^{\circ}C$. Optimun concentration of sodium chloride was $2.0\%$ for the callus growth in PES-agar medium.

• Journal of Life Science
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• v.9 no.5
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• pp.531-536
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• 1999

As an attempt to preserve resources in marine biological organisms, we first constructed a cDNA library from the red alga Porphyra yezoensis. The library construction method from P. yezoensis consists of three steps; those include protoplast presparation, RNA isolation, and phage library construction. Protoplast was prepared in order to remove much of the carbohydrate compounds which are characteristics of algal cell walls. Carbohydrate contamination in the purified RNA may inhibit further enzyme reactions, those carbohydrates should be removed. RNA samples prepared from protoplast still seemed to contain residual amount of carbohydrate because mRNA isolation with conventional method failed. We therefore developed a method with Poly ATtract mRNA isolation system. The constructed phage library was tested by analyzing cDNA insert in phage vector from randomly picked ten independent white plagues. All of the phages contained cDNA inserts with sizes ranging 0.5kb and 2.0kb.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.765-769
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• 2014

We examined the protective effects of Pyropia yezoensis glycoprotein (PYGP) against ethanol-induced gastric damage. The experimental animals were divided into four groups. They were treated with distilled water (control), ethanol alone (EtOH), ethanol + PYGP 150 mg/kg BW (EtOH+150), or ethanol + PYGP 300 mg/kg BW (EtOH+300). The groups were treated for 4 weeks. We measured mitogen-activated protein kinase (MAPK), the apoptotic signaling pathway, and PARP activity in gastric tissues obtained from the rats. Ethanol consumption increased apoptotic signal activity and ERK, JNK, and p38 phosphorylation. PYGP reduced the apoptotic signaling pathway activity and ERK, JNK, and p38 phosphorylation. Furthermore, PYGP regulated Bcl-2 family expression. In light of these findings, PYGP appears to prevent ethanol-induced gastric injury and oxidative stress.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.118-118
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• 2003

홍조류 김속 해조 5종(모무늬돌김, 방사무늬김, 참김, 긴잎돌김, 잇바디돌김)의 사상체를 2단계 냉각법으로 액체질소 중에서 동결보존을 시행하였다. 시료를 여러 가지 동해보호제에 현탁시킨 후 프로그램 냉각기로 4시간에 걸쳐 -4$0^{\circ}C$까지 천천히 동결시켰다. 일차 완만동결 종결 후 즉시 동결용 튜브를 액체질소 중에 수용하여 급속동결 시켰다. 해동시에는 4$0^{\circ}C$의 항온수조에서 대부분의 얼음 결정을 급격히 해동시킨 후 냉각수내에서 완전히 해동시켰다. 생존률은 김 속 해조에서는 neutral red로 염색하여 산정하였으며 50% 해수에 10% DMSO와 0.5M sorbitol 혼합액을 동해보호제로 사용하였을 때의 생존율이 54.6~70.9%였다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.757-764
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• 2014

Many researchers have studied algae as a source of material having potential biological activities, not least because many marine algae extracts have strong antioxidant properties. In this study, we investigated the anti-inflammatory effects of Pyropia yezoensis extract (PYE) on RAW 264.7 cells by measuring nitric oxide (NO), reactive oxygen species (ROS), superoxide dismutase (SOD), catalase activity, inducible NOS (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-${\kappa}B$), interleukin-$1{\beta}$ (1L-$1{\beta}$), and tumor necrosis factor-alpha (TNF-${\alpha}$). PYE decreased the production of intracellular ROS dose-dependently and increased SOD and catalase activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. PYE significantly suppressed the production of NO and reduced the expression of iNOS, COX-2, and NF-${\kappa}B$. PYE treatment also inhibited the production of IL-$1{\beta}$ and TNF-${\alpha}$ significantly and reduced the phosphorylation of Akt and MAPK significantly in LPS-stimulated RAW 264.7 cells. These results suggest that PYE has potential anti-oxidant and anti-inflammatory activities.

• Korean Journal of Environment and Ecology
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• v.30 no.1
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• pp.98-103
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• 2016

Pyropia yezoensis were sampled from Jeonnam in Korea, Jiansu in China and Saga in Japan. Sampled P. yezoensis were analyzed proximate composition by Association official Analytical Chemists(AOAC) method, minerals by ICP-OES and amino acid by amino acid analyzer. Three areas resulted that contents of moisture and crude protein were $8.13{\pm}0.38%$ and $37.25{\pm}1.15%$, and crude lipid, ash and carbohydrate were $2.12{\pm}0.27%$, $7.41{\pm}0.36%$ and $45.06{\pm}1.30%$ respectively. In total minerals, Korea and China were $28300.19{\pm}76.39ppm$and $13945.63{\pm}7.73ppm$, and Japan was $12262.485{\pm}4.38ppm$. Amino acids, Korea and China were $265.28{\pm}0.38mg\;g^{-1}$ and $209.19{\pm}0.19mg\;g^{-1}$, and Japan was $157.61{\pm}0.43mg\;g^{-1}$. Therefore, those substance contents of P. yezoensis are significantly different between cultivation sites. The basic data may used to the environmental and nutritional study of P. yezoensis based on country sites.

• Journal of Marine Bioscience and Biotechnology
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• v.15 no.2
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• pp.33-40
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• 2023

This study aimed to investigate the immunomodulatory function of Pyropia yezoensis hydrothermal (water) extract (PYWE) in comparison to the group treated only with lipopolysaccharides (LPS) in RAW264.7 cells. LPS is known to be an inflammatory mediator that activates macrophages, leading to the secretion of nitric oxide (NO), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) as defense responses. Through enzyme-linked immunoassay and western blot analyses, it was observed that PYWE increased the expression levels of NO, iNOS, TNF-α, and IL-6 in RAW264.7 cells in a dose-dependent manner, although to a lesser extent compared with the group treated with LPS alone. In addition, the study examined the mitogen-activated protein kinases (MAPKs) pathway, which regulates various cellular activities, including gene expression, mitosis, cell differentiation, transformation, survival, and death. The western blot analysis confirmed that PYWE also regulated the MAPKs pathway. Furthermore, the expression levels of immunomodulatory-related factors increased in the group treated with PYWE compared with the control group. Even though the effects of PYWE were usually less strong than those of LPS, the effects of PYWE increased with increasing doses compared to the control group. This suggests that PYWE could be used to control the immune system.