Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Immune Enhancing Effects of Pyropia yezoensis Hydrothermal Extract in RAW 264.7 Cells

방사무늬 김 열수추출물의 RAW 264.7 세포에서의 면역 증진 효과

  • Goeun Jang (Marine Healthcare Research & Evaluation Center, Chosun University) ;
  • Bo-Ram Park (Department of Dental Hygiene, College of Health and Welfare, Kyungwoon University) ;
  • Seul Ah Lee (Marine Healthcare Research & Evaluation Center, Chosun University) ;
  • Chun Sung Kim (Marine Healthcare Research & Evaluation Center, Chosun University)
  • 장고은 (해양헬스케어 유효성실증센터) ;
  • 박보람 (경운대학교 보건복지대학 치위생학과) ;
  • 이슬아 (해양헬스케어 유효성실증센터) ;
  • 김춘성 (해양헬스케어 유효성실증센터)
  • Received : 2023.06.07
  • Accepted : 2023.07.29
  • Published : 2023.12.31
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Abstract

This study aimed to investigate the immunomodulatory function of Pyropia yezoensis hydrothermal (water) extract (PYWE) in comparison to the group treated only with lipopolysaccharides (LPS) in RAW264.7 cells. LPS is known to be an inflammatory mediator that activates macrophages, leading to the secretion of nitric oxide (NO), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) as defense responses. Through enzyme-linked immunoassay and western blot analyses, it was observed that PYWE increased the expression levels of NO, iNOS, TNF-α, and IL-6 in RAW264.7 cells in a dose-dependent manner, although to a lesser extent compared with the group treated with LPS alone. In addition, the study examined the mitogen-activated protein kinases (MAPKs) pathway, which regulates various cellular activities, including gene expression, mitosis, cell differentiation, transformation, survival, and death. The western blot analysis confirmed that PYWE also regulated the MAPKs pathway. Furthermore, the expression levels of immunomodulatory-related factors increased in the group treated with PYWE compared with the control group. Even though the effects of PYWE were usually less strong than those of LPS, the effects of PYWE increased with increasing doses compared to the control group. This suggests that PYWE could be used to control the immune system.

Keywords

Acknowledgement

이 논문은 2021년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구입니다(20210656, 빅데이터 기반 해양바이러스 제어 및 마린바이오틱스 개발사업).

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