Song, Ki Seon;Jeon, Kwon Seok;Kim, Chang Hwan;Yoon, Jun Hyuck;Park, Yong Bae;Kim, Jong Jin
Journal of Bio-Environment Control
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v.23
no.2
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pp.88-94
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2014
This study was carried out to survey the early growth characteristics of Ligularia fischeri, plants of half-shaded grounds, primarily used as functional wild edible greens and examine its shading treatment to transplant seedling. The shading treatment was regulated with the shading level(full sunlight, 35%, 50%, 75%, and 95% of full sunlight). According to the experiment, the height was the highest under 75% of shading (17.3 cm), and root diameter was the highest under full sunlight (2.13 mm). It was found that fresh weight (leaf, shoot, root and whole) was the highest (1.784 g, 1.330 g, 0.791 g, 3.905 g respectively ) under 50% of shading. In case of dry weight, leaf, shoot and whole dry weight were surveyed the highest under 35% of shading, and root dry weight was the highest under full sunlight. It was found that S/R ratio and moisture contents (leaf, shoot, root and whole) were the highest under 95% of shading. Leaf area was the highest under 50% of shading ($39.3cm^2$) and the higher shading level, the higher SLA and LAR. It was surveyed that the higher shading level, the thinner leaf thickness. In case of root characteristics, it was showed that the relatively high height growth under 35%~75% of shading, excepting 95% of shading. As a result of surveying the whole experiment, it is concluded that the early growth of L. fischeri is more effective in producing it under 50% of shading.
Wetland plants have evolved specialized adaptations to survive in the low-oxygen conditions associated with prolonged flooding. The development of internal gas space by means of aerenchyma is crucial for wetland plants to transport $O_2$ from the atmosphere into the roots and rhizome. The formation of tissue with high porosity depends on the species and environmental condition, which can control the depth of root penetration and the duration of root tolerance in the flooded sediments. The oxygen in the internal gas space of plants can be delivered from the atmosphere to the root and rhizome by both passive molecular diffusion and convective throughflow. The release of $O_2$ from the roots supplies oxygen demand for root respiration, microbial respiration, and chemical oxidation processes and stimulates aerobic decomposition of organic matter. Another essential mechanism of wetland plants is downward water movement across the root zone induced by water uptake. Natural and constructed wetlands sediments have low hydraulic conductivity due to the relatively fine particle sizes in the litter layer and, therefore, negligible water movement. Under such condition, the water uptake by wetland plants creates a water potential difference in the rhizosphere which acts as a driving force to draw water and dissolved solutes into the sediments. A large number of anatomical, morphological and physiological studies have been conducted to investigate the specialized adaptations of wetland plants that enable them to tolerate water saturated environment and to support their biochemical activities. Despite this, there is little knowledge regarding how the combined effects of wetland plants influence the biogeochemistry of wetland sediments. A further investigation of how the Presence of plants and their growth cycle affects the biogeochemistry of sediments will be of particular importance to understand the role of wetland in the ecological environment.
We clarified the background for establishment of vegetation by comparing the spatial distribution maps of vegetation and substrate on a gravel bar in the Bangtae stream located on Inje-gun of Gangwon-do, the central eastern Korea. The total vegetation coverage was higher in the interior and lower in the marginal parts of the gravel bar. Spatial distribution of vegetation on the longitudinal section of the gravel bar tended to be arranged in the order of shrub, subtree, and tree dominated vegetation types from the front (upstream) toward the rear (downstream) parts. Coverage of the herbaceous plants was higher in the central and rear parts and lower in the front and right parts of the gravel bar. Vegetation height was higher in the rear part and became lowered as move toward the front part. Substrate was distributed in the order of boulder, gravel, sand, and boulder from the front toward the rear parts. Ordination of stands based on vegetation data was arranged in the order of annual plant, perennial herb, shrub, and tree dominated vegetation as move from the right to the left parts on the axis I. Species richness was higher in the order of Pinus densiflora community, Phragmites japonica community, Salix gracilistyla community, Fraxinus rhynchophylla community, annual plant dominated vegetation, and Prunus padus for. padus community based on the species rank-abundance curve. The order based on the Shannon's index was some different; diversity of Phragmites japonica community and Salix gracilistyla community, which showed higher dominance degree, were low differently from species richness. In conclusion, it was evaluated that the gravel bar newly established toward the upstream and vegetation dynamics of the gravel bar seemed to follow ecosystem mechanisms of succession. As were shown in the above results, the Bangtae stream corresponded to the upstream and thereby particle size of substrate was big. Therefore, they move by rolling and are accumulated for the upstream. Vegetation types were arranged in the order of woodland, shrub-land and grassland from the rear toward the front parts of the gravel bar and thereby reflected the formation process of the bar. However, the gravel bar is disturbed frequently by not only the running water but also the suspended sand as the dynamic space. Such disturbances cause habitat diversity and consequently led to high biodiversity.
Park, Se-Ah;Kang, Hyun-Mi;Heo, Jin-Yeong;Yoon, Jin-Ah;Kim, Hae-Kwon
Clinical and Experimental Reproductive Medicine
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v.36
no.1
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pp.23-34
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2009
Objectives: Mesenchymal stem cells (MSC) comprise a promising tool for cellular therapy. It is known that long-term in vitro culture of human bone marrow and adipose tissue derived-MSCs lead to a reduction of life span and a change of stem-like characters. The aim of our study was to examine whether stem cell properties of human umbilical cord-derived stem cells (HUC) could be affected by in vitro expansion. Methods: HUC were isolated from human umbilical cord and cultured for 10 passages in vitro. Morphology and population doubling time (PDT) were investigated, and changes of stem cell properties were examined using RT-PCR and immunocytochemistry during serial subcultures. Results: Morphology and PDT of HUC began to change slightly from the 7th passage (p7). Expression level of nestin and vimentin mRNAs increased along with the culture period from p4 until p10. In contrast, expression level of SCF mRNA decreased during the same culture period. Expression level of Oct-4 and HNF-4${\alpha}$ mRNAs was not significantly changed throughout the culture period until p10. Expression level of BMP-4, FGF-5, NCAM and HLA-ABC mRNAs appeared to increase as the culture continued, however, the difference was not significant. Immunocytochemical studies showed that HUC at p3, p6 and p9 positively were stained with antibodies against SSEA-3 and SSEA-4 proteins. Interestingly, staining intensity of HUC for ICAM-1 and HLA-ABC gradually increased throughout the culture period. Intensity against thy-1 and fibronectin antibodies increased at p9 while that against TRA-1-60 and VCAM-1 antibodies began to decrease at p6 until p9. Conclusions: These results suggest that HUC change some of their stem cell characteristics during in vitro culture. Development of culture system might be needed for the maintenance of characteristics.
The purpose of present the study was to describe the spawning behavior and early life history of the grass puffer, Takifugu niphobles. The grass puffer was caught at the spawning ground on the coast Dolsan, Korea from May to the 1998. Artificial fertilization was carried out to get fertilied eggs. Spawning season was from the end of May to the beginning of July on the coast of the Dolsan, Korea. Form 2~6 days before the spring tide the aduct males and females began to aggregate in the shore water, and they spawned in the upper part of intertidal zone exposed to air among pebble stone. The fertilized eggs were demersal, transparent and spherical in shape, and its diameter were 0.87~0.95 mm with several oil globules of 0.0014~0.0540 mm. Hatching began about 146 hours after fertilization under water temperature $21.2{\sim}22.3^{\circ}C$. The newly-hatched larva was 2.02~2.24 mm in total length (TL, mean: 2.14 mm), with 22~24(8~9+14~15) myotomes, and mouth and anus was not yet open. In 3 days after hatching it was measured 2.64~2.93 mm in TL (mean: 2.76 mm), and yolk sac was completely absorbed and transformed to postlarval stage. In 15 days after hatching it was measured 4.43~4.82mm in TL (mean: 4.59mm), and the caudal notochord flexion started. The larvae reached the juvenile stage in 28 days after hatching and attained 7.55~7.65mm in TL (mean: 7.60mm), and all fin-rays were formed.
The coal-bearing siliciclastic rocks of the Lower Permian Jangseong Formation, Samcheog coalfield, represent a megacyclothem which shows cyclic repetitions of sandstone, shale, coaly shale, and coals. Petrographic, geochemical, and SEM studies for sandstone samples, and XRD analysis for clay minerals were carried out to understand diagenesis in the sandstones of the Jangseong Formation. The Jangseong sandstones are composed of 60% quartz (mainly monocrystalline quartz) and 36% clay matrix and cement with minor amounts of feldspar, lithic fragments and accessory minerals (less than 4%). Jangseong sandstones are classified mostly as quartzwackes and partly as lithic graywackes according to the scheme of Dott(1964). The textural relationships between authigenic minerals and cements in thin sections and SEM photomicrographs suggest the paragenetic sequence as follows; (1) mechanical compaction, (2) cementation by quartz overgrowth, (3) formation of authigenic clay minerals (illite, kaolinite), (4) dissolution of framework grains and development of secondary porosity, and (5) later-stage pore-filling by pyrophyllite. We propose that these diagenetic processes might be due to organic-inorganic interaction between the dominant framework grains and the formation water. The Al, Si ions and organic acid, derived from dewatering of interbedded organic-rich shale and coals, were transported into the Jangseong sandstones. This caused changes in the chemistry of the formation water of the sandstones, and resulted in overgrowth of quartz and precipitation of authigenic clay minerals of kaolinite and illite. The secondary pores, produced during dissolution of clay and framework grains by organic acid and $CO_2$ gas, were conduit for silica-rich solution into the Jangseong sandstones and the influx of silica-rich solution produced the late-stage pyrophyllite after the expanse of kaolinite. The origin of the solution that formed pyrophyllite is not likely to be the organic-rich formation water based on the observation of fracture-filling pyrophyllite in the Jangseong sandstones, but the process of pyrophyllite pore-filling was indirectly related to organic-inorganic interaction.
In the amphibolites of the Hwanggangri area, three metamorphic zones are established like hornblende-actinolite zone (H-AZ), hornblende zone (HZ) and diopside zone (DZ) by the main mineral assemblages. Hornblende zone and hornblende-actinolite zone develope away from the diopside zone that experienced the highest thermal effect. Thus, this pattern identifies the decreasing metamorphic grade of the contact metamorphism with increasing distance from the granitic pluton. The mineral assemblages of this rock are classified into six representative groups such as $\circled1$ actinolite+plagioclase+chlorite, $\circled2$ actinolite+hornblende+plagioclase+chlorite$\pm$epidote$\pm$biotite, $\circled3$ actinolite+hornblende+plagioclass$\pm$biotite$\pm$epidote, $\circled4$ hornblende+plagioclase$\pm$biotite$\pm$chlorite, $\circled5$ hornblende+plagioclase+diopside+actinolite$\pm$epidote$\pm$chlorite, $\circled6$hornblende+plagioclase+diopside$\pm$biotite$\pm$epidote. Two metamorphic events m recognized in the amphibolites of the study area that the first metamorphism is the regional metamorphism dominantly occurred in the whole Ogcheon metamorphic belt and it gave rise to the growth of actinolite at the core or center of the amphibole grains of coarse and medium size. Its metamorphic grade ranges from the greenschist facies to epidote-amphibolite facies. The second metamorphism overlapped is the contact metamorphism caused by the adjacent granitic pluton, and its metamorphic grade is thought to reach to the low pressure part of upper amphibolite facies. According to the calculation by TWEEQU thermobarometry and amphibole-plagioclase thermometry, the metamorphic temperature of initial regional metamorphism is $439-537^{\circ}C$ under pressure of 4.6-7.3 kb and its peak temperature and pressure are considered to reach to the range of 492-537 and 5.2-7.3 kb. And the temperature range of contact metamorphism occurred by intrusion of cretaceous granitic body, is $588-739^{\circ}C$ under pressure of 2.6-5.2 kb and its peak temperature and pressure are estimated as having the range of $697-739^{\circ}C$ and 3.8-5.2 kb that this amphibolites are estimated to pass through the metamorphic evolution of both the rise of temperature and the drop of pressure.
The present study identified the restrictions on the use of sous-vide products in the Korean HMR market for small and medium-sized manufacturing companies. A detailed literature review revealed that the HMR market in Korea is close to saturation. Notably, the technologically advanced products produced using sous-vide seem to display significant potential to overcome market saturation. The sous-vide method differs from conventional cooking techniques and is characterized by maintenance of food texture along with flavor enhancement. However, due to the unfamiliarity of the manufacturers with this method and the unclear food safety regulations, mass food manufacturing companies do not agree on using this method; hence, sous-vide production is usually undertaken by small/medium sized companies catering primarily through online marketing portals. This study highlights the various restrictions to the implementation of sous-vide production, and discusses several practical implications of sous-vide production that would help users of this technique enter the HMR market.
The purpose of this study was to analyze about STS contents in the next generation science textbook for 10th grade according to curriculum revision 2007 and high school science textbooks focused on the Earth Science which were published according to the 7th curriculum. The contents of STS were analyzed by the STS topics of Yager(1989), Piel's standard(1981), and student activities by SATIS. The results of this study are the same as follows: 'The next generation science textbook' was shown that 20.9% is STS material amount in average by Yager's standard. 'High school science textbooks' were shown that 11.3% is STS material amount in average. Based on the STS topics by Yager's standard, most of STS content is focused on 'Relativity with local community', 'Application of science' and 'Cooperative work on real problems'. However, there is rare contents such as 'Multiple dimensions of science', 'Practice with decision-making strategies' and 'Evaluation concerned for getting and using information' in the next generation science textbook. In high school science textbooks were shown that 'Applicability of science' is the highest and 'Relativity with local community' is the next high contents. Based on the STS topics by Piel's standard, most of STS contents are focused on 'Environmental quality', 'Space research' and 'National defence' in the next generation science textbook. But high school science textbooks are focused on 'Natural resources' and 'Technology development'. The activities were analyzed by SATIS student activities. The major categories of activities included in the next generation science textbook were 'Investigation', 'Simulation' and 'Data analysis'. But, there were rare activities like 'Roleplaying', 'Research design' and 'Simulation' in high school science textbooks.
Astronomy is the subject that can easily draw students' interest in studying science, therefore plays an important role in developing scientific core competence. However, it is difficult to develop spatial thinking that is required in understanding astronomical concepts through the classroom lessons. Planetarium, along with the science museum, is one of the most representative informal educational institution where astronomical concepts can be taught beyond time and space constraints. In this study, we developed the astronomy education program that is operated in the planetarium and applied the program to the elementary, middle and high-school students to investigate the effects of the planetarium lesson compared to the classroom lesson. The duration of this research was about 8 months, and the number of the students involved is 761, including participants of the 1 night and 2 days camps in the Daegu National Science Museum. The newly developed planetarium lesson is comparable to the previous classroom lesson of which topics are cardinal points, constellation, and the rotation of the earth. Test items were developed to evaluate the understanding of the astronomical concepts. The study was conducted based on the pre- and post-test with non-equivalent groups design comparing classroom and planetarium lessons. The results of this study are as follows. First, planetarium lesson is more effective for understanding astronomical concepts such as the cardinal points, earth's rotation, and the constellation than classroom lesson. Second, planetarium has a positive effect irrespective of gender and previous knowledge. Third, planetarium for high school students has the same effect as additional observation activities followed by the classroom instruction. Therefore, planetarium can be used as an alternative, effective tool when night observation is not available. In summary, planetarium is an effective tool that helps students to understand the astronomical concepts.
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