• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.505-506
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• 2010

• The Korean Journal of Food And Nutrition
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• v.36 no.1
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• pp.42-49
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• 2023

To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.

• Korean Journal of Environment and Ecology
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• v.18 no.4
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• pp.382-391
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• 2004

This study was conducted to establish the effective and systematic counter plan of conservation by analyzing vegetation on Korean endemic plants and rare and endangered plants, Abeliophyllum distichum, habitats and to present the raw data and direction on counter plan of restoration. The dominant species of tree layers were Platycarya strobilacea(Goesan-gun Chujeom-ri I), Pinus densiflora(Goesan-gun Songdeok-ri II and Yulji-ri III), Robinia pseudo-acacia(Yeongdong-eup Maechon-ri IV), Quercus variabilis(Jincheon-gun Yongjeong-ri V) and Pinus densiflora$.$Quercus variabilis(Buan-gun Junggye-ri Ⅵ). Subtree layers were dominated by Quercus dentata(1st site), Quercus serratu(2nd site), Quercus acutissima(3rd site), Robinia pseudo-acacia(4th site), Zelkova serrata(5th site) and Quercus variabilis(6th site). And the dominant species of shrub layers were Lindera obtusiloba(1st and 5th site), Lespedeza bicolor(2nd site), Stephunandra incisa(3rd site), Quercus aliena(4th site) and Rhododendron schlippen-bachii(6th site). The dominant species of herb layers were surveyed Humulus japonicus$.$Pueraria thunbergiana(1st site), Oplismenus undulatifolius(2nd and 4th site), Carex siderosticta$.$Pueraria thunbergiana(3rd site), Streptolirion cordifolium$.$Humulus japonicus(5th site) and Sasa borealis(6th site). Among the surveyed sites, the Goesan-gun Yulji-ri supremely abounded in species and individuals, and there was surveyed each 21 species and 116 individuals. As well, this site showed the highest H'(1.123) and H'$_{max}$(1.322). Therefore, Goesan-gun Yulji-ri formed diverse vegetation and Yeongdong-eup Maechon-ri and Buan-gun Junggye-ri showed relatively poor vegetation.ation.

• Korean Journal of Plant Resources
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• v.18 no.2
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• pp.315-324
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• 2005

The study was carried out to offer the raw data on counterplan of restoration and conservation by systematical and objective analyzing the flora and herbaceous communities around Abeliophyllum distichum habitats. In the results of flora survey, as the site that the most taxa were showed was Yulji-ri, Geosan-gun III, there was surveyed as 114 ones; 48 families, 91 genera, 99 species and 15 varieties, and as Younjeong-ri, Jincheon-gun V was showed the fewest ones, there was appeared 54 ones; 28 families, 49 genera, 47 species and 7 varieties. The dominant species of herbaceous communities were verified Humulus japonicus${\cdot}$Artemisia princeps var. orientalis(Chujeom-ri, Goesan-gun I), Oplismenus undulatifolius(Songdeok-ri, Goesan-gun II and Maechon-ri, Yeongdong-eup IV), Carex siderosticta${\cdot}$Pueraria thunbergiana${\cdot}$Artemisia princeps var. orientalis(Yulji-ri, Geosan-gun III), Streptolirion cordifolium(Younjeong-ri, Jincheon-Eun V) and Sasa borealis(Junggye-ri, Buan-gun IV). In correlation analysis, Artemisia princeps var. orientalis${\cdot}$Carex siderosticta, Sasa borealis${\cdot}$Festuca ovina, Sasa borealis${\cdot}$Smilax china and Festuca ovina${\cdot}$Smilax china were showed the highest relativity. In similarity index analysis, as the site that was showed highest value was Yulji-ri, Geosan-gun III and Maechon-ri, Yeongdong-eup IV, there was turned up about some $27.27\%$. In the results of regression analysis between environmental factors and number of species, R-square of altitude and number of species was some $70.0\%$.

• Journal of Convergence for Information Technology
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• v.10 no.3
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• pp.76-91
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• 2020

Whole genome sequence of Abeliophyllum distichum Nakai (Oleaceae) cultivar Ok Hwang 1 Ho, which is Korean endemic species, was recently sequenced to understand its characteristics. Acteoside is one of major useful compounds presenting various activities, and its several proposed biochemical pathways were reviewed and integrated to make precise biochemical pathway. Utilizing MetaPre-AI^TM which was developed for predicting secondary metabolites based on whole genome with the precise biochemical pathway of acteoside and the InfoBoss Pathway Database, we successfully rescued all enzymes involved in this pathway from the genome sequences, presenting that A. distichum cultivar Ok Hwang 1 Ho may produce acteoside. High-performance liquid chromatography result displayed that callus of A. distichum cultivar Ok Hwang 1 Ho contained acteoside as well as isoacteoside which may be derived from acteoside. Taken together, we successfully showed that MetaPre-AI^TM can predict secondary metabolite from plant whole genomes. In addition, this method will be efficient to predict secondary metabolites of many plant species because DNA can be analyzed more stability than chemical compounds.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.219-225
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• 2011

Changes in biota, species distribution range shift and catastrophic climate influence due to recent global warming have been observed during the last century. Since global warming affects various sectors, such as agriculture and vegetation, it is important to predict more accurate impact of future climate change. The purpose of this study is to examine the observed distribution of Abeliophyllum distichum in the Korean peninsula. For this purpose, two period (present and future) climate data were used. Mean data between 1950 and 2000, were used as the present value and the year 2050 and 2080 data from A1B senario in IPCC SRES were used for the future value. Potential habitation is analyzed by MaxEnt(Maximum Entropy model), and Abeliophyllum distichum's coordinates data were used as a dependent variable and independent variables are composed of environmental data such as BioClim, altitude, aspect and slope. The result of six types GCM mean calculation, the potential habitability decreased by 40-60% of the average existing distribution. The methodogies and results of this research can be applicable to the climate changing adaptation stratiegies for the biodiversity conservation.

• The Korea Journal of Herbology
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• v.26 no.4
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• pp.95-99
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• 2011

Objective : In this study, we demonstrate the protective effect on oxidative DNA damage of leaf extracts from Abeliophylli distichi Folium via its antioxidant activity for the establishment of new value for the herbal medicine. Methods : Abeliophylli distichi Folium leaves were extracted with hot-water and ethylacetate (EtOAC). The 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical and hydroxyl scavenging assay and $Fe^{2+}$ chelating assay were performed for antioxidative effect and ${\varphi}$X-174 RF I DNA cleavage assay and intracellular DNA damage assay were used for inhibitory effect of intracellular DNA damage. Results : In DPPH, Hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity of EtOAC extracts were 94.72%, 62.88%, 41.13%, and hot-water extracts were 88.86%, 56.7%, 37.4% at 200 ${\mu}g/m{\ell}$, respectively. Also, those extracts showed protective effect of DNA damage against the oxidative stress. Conclusion : These results indicated that the leaf extracts of Abeliophylli distichi Folium can be used as a natural antioxidants, which effectively inhibits the oxidative DNA damage.

• Korean Journal of Medicinal Crop Science
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• v.23 no.2
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• pp.155-160
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• 2015

This research evaluate antioxidant and skin-whitening effect of Abeliophyllum distichum Nakai by extraction processes. First, antioxidant effects were follows: EE (70% ethanol extract) showed higher DPPH scavenging activity of 69.66% than WE (hot water exract) 59.13% at $0.3mg/m{\ell}$, also UE's (70% ethanol extract by sonication process) higher than EE. Reducing power was that also EE showed higher than WE, and it was the highest value with UE's because of ultrasonic pretreatment. Next, the whitening effect tyrosinase inhibition activity was measured that EE was 23.88%, WE's was 16.69%, and UE was 23.34%. Ultrasonic pretreatment did not influence to tyrosinase inhibition activity. Cell viability showed low cell toxicity in all groups. UE's inhibited melanin synthesis, 55.1%, that is higher than EE and WE, 52.7% and 39.5%, respectively. As a result, we confirmed that antioxidant activities and skin-whitening effect by extraction process. Also, this results confirmed that the Abeliophyllum distichum Nakai extracts worth as cosmetic materials.

• Journal of the Korean Society of Food Culture
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• v.34 no.6
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• pp.801-809
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• 2019

Abeliophyllum distichum Nakai is a deciduous shrub of a flowering plant in Oleaceae. It is an important plant resource and consists of only one species in the entire world. A. distichum Nakai is well known an edible, medicinal herb in its habitat districts, but the toxicological evaluation for the safe use of its extract is still insufficient. The study characterized the toxicity of an Abeliophyllum distichum Nakai ethanol extract in Sprague-Dawley (SD) rats and determined the safe dosage levels in a 13 weeks toxicity study. Abeliophyllum distichum Nakai ethanol extract was orally administered once daily for 2 weeks at 0, 500, 1,000 and 2,000 mg/kg/day to male and female SD rats. while recording the clinical signs of toxicity, body weight, food intake/consumption, eye test and urine analysis. Only the total protein frequency in the urine of male SD rats (p<0.05), the right ovary of the 500 mg/kg group (p<0.01) and the right adrenal gland of the 1,000 mg/kg group (p<0.05) in the female rats showed statistically significant changes. But no toxic effects were noted from repeated-dose administration of the Abeliophyllum distichum Nakai ethanol extract in the SD rats during the observation period. The post-mortem examinations showed no test substance-mediated changes. The hematological analysis and clinical blood chemistry data demonstrated no toxic effects from repeated-dose administration of Abeliophyllum distichum Nakai ethanol extract in the SD rats during the observation period. Based on these results, this data suggests that a dose of 1,000 mg/kg/day is a highest treatment to administer when conducting a further 13 weeks toxicity study.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.231-236
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• 2015

Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at $100^{\circ}C$, 70% ethanol at $85^{\circ}C$, and 70% ethanol with ultrasonication at $60^{\circ}C$ The ultrasonicated extract showed 95.62% of the highest cell viability in addition of $0.3mg/m{\ell}$ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to $2908.1pg/m{\ell}$ with ultrasonicated extract while $6640.8pg/m{\ell}$ with water extract and $3609.3pg/m{\ell}$ with ethanol extract, in addition of $0.3mg/m{\ell}$. Collagen production was increased up to $154.7ng/m{\ell}$ in addition of ultrasonicated extract, and followed by $121.4ng/m{\ell}$ and $31.2ng/m{\ell}$ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication.