Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Prediction and Identification of Biochemical Pathway of Acteoside from Whole Genome Sequences of Abeliophyllum Distichum Nakai, Cultivar Ok Hwang 1ho

미선나무 품종 옥황 1호의 유전체를 활용한 Acteoside 생화학 합성과정 예측 및 확인

  • Park, Jaeho (Department of Pharmaceutical Science, JungWon University) ;
  • Xi, Hong (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Han, Jiyun (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Lee, Jeongmin (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Kim, Yongsung (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Lee, Jun-mi (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Son, Janghyuk (InfoBoss Co., Ltd. & InfoBoss Research Center) ;
  • Ahn, Joungjwa (Department of Food Science & Technology, JungWon University) ;
  • Jang, Taewon (Department of Medicinal Plant Resources, Andong National University) ;
  • Choi, Jisoo (Department of Pharmaceutical Science, JungWon University) ;
  • Park, Jongsun (InfoBoss Co., Ltd. & InfoBoss Research Center)
  • 박재호 (중원대학교 제약공학과) ;
  • 시홍 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 한지윤 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 이정민 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 김용성 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 이준미 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 손장혁 (인포보스 주식회사 및 인포보스 기업부설연구소) ;
  • 안정좌 (중원대학교 식품공학과) ;
  • 장태원 (안동대학교 생약자원개발학과) ;
  • 최지수 (중원대학교 제약공학과) ;
  • 박종선 (인포보스 주식회사 및 인포보스 기업부설연구소)
  • Received : 2020.01.06
  • Accepted : 2020.03.20
  • Published : 2020.03.28
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Abstract

Whole genome sequence of Abeliophyllum distichum Nakai (Oleaceae) cultivar Ok Hwang 1 Ho, which is Korean endemic species, was recently sequenced to understand its characteristics. Acteoside is one of major useful compounds presenting various activities, and its several proposed biochemical pathways were reviewed and integrated to make precise biochemical pathway. Utilizing MetaPre-AITM which was developed for predicting secondary metabolites based on whole genome with the precise biochemical pathway of acteoside and the InfoBoss Pathway Database, we successfully rescued all enzymes involved in this pathway from the genome sequences, presenting that A. distichum cultivar Ok Hwang 1 Ho may produce acteoside. High-performance liquid chromatography result displayed that callus of A. distichum cultivar Ok Hwang 1 Ho contained acteoside as well as isoacteoside which may be derived from acteoside. Taken together, we successfully showed that MetaPre-AITM can predict secondary metabolite from plant whole genomes. In addition, this method will be efficient to predict secondary metabolites of many plant species because DNA can be analyzed more stability than chemical compounds.

최근에 한국 고유종인 미선나무 (Abeliophyllum distichum Nakai; Oleaceae) 품종 옥황1호의 유전체가 성공적으로 해독되었다. Acteoside는 다양한 활성을 가지는 물질이며, 여러개의 생화학합성과정이 제시되어왔고, 이들을 통합 검토하여 정확한 생화학합성과정을 완성하였다. 유전체 데이터로부터 2차대사산물을 예측할 수 있는 MetaPre-AITM와 정확한 acteoside 생화학합성과정, InfoBoss Pathway Database를 활용하여, acteoside에 관여하는 모든 효소의 유전자를 옥황1호 유전체로부터 성공적으로 확인하였다. 이는 옥황1호는 acteoside 물질을 생산할 수 있는 가능성이 있음을 의미한다. 이에 고성능액체크로마토그래피를 사용하여 옥황1호의 캘러스 세포를 분석하여 acteoside과 이의 유도체인 isoacteoside를 확인하였다. 본 연구는 MetaPre-AITM은 유전체로부터 2차대사산물을 성공적으로 예측하였다. 이 방법은 화학물질보다 안정적인 DNA를 분석하여 2차 대사산물을 예측하는 효율적인 방법이 될 것이다.

Keywords

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