• Korean journal of food and cookery science
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• v.14 no.1
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• pp.1-8
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• 1998

The characteristics of Kimchi, associated with proceeding velocity of fermentation, and microbial changes, were investigated during fermentation where kimchi were prepared by the addition of different kinds of minor ingredients. In all samples, total acidity increased, and the pH and total reducing sugar content decreased as the fermentation proceeded. Redox potentials decreased from early to middle fermentation period but thereafter increased and had the lowest value at optimum ripening stage. The counts of total viable and lactic acid bacteria increased rapidly at the begining and decreased slowly. These bacterial counts of kimchi samples containing ginger, red pepper powder, and mixture of all minor ingredients were higher than those of other samples. The number of Leuconostoc mensenteroides reached the maximum at the optimum fermenting stage, Lactobacillus plantarum appeared at the time when Leu. mesenteroides reduced. All these phenomena were observed earlier in the samples containing raw red pepper and later in the samples added garlic than in other samples. It is suggested that garlic is the ingredient which improved storage capacity by prolonging fermentation period by lactic acid bacteria beneficial to less acidification, and ginger and red pepper powder are materials which gave better taste to kimchi by stimulating the growth of Leu. mesenteroids. And redox potential was an useful indicator in choosing optimum ripening time during fermentation.

• Korean Journal of Microbiology
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• v.44 no.4
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• pp.277-281
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• 2008

HIV affects many organ systems. Patients with HIV infection have substantially increased risk of developing various cancers, primarily by opportunistic infection with oncogenic viruses due to their immunocompromised status. However, extensive evidence also indicates that the viral protein, Tat itself, may playas a major factor in the development of AIDS-related neoplasms. The molecular mechanism underlying Tat's oncogenic activity may include deregulation of cellular genes. Therefore, in this study, we examined the effect of HIV-l Tat on CD99 as one of the target cellular genes, which is a well-known tumor marker in several cancers. By using established HeLa clones that are stably expressing Tat, we found that CD99 is upregulated by endogenous Tat, whereas STAT3 is down regulated. Upon the screening of genes differentially expressed between Tat-stable cells and the control cells by using the gene fishing technique, DEG, we detected 3 genes which expression is affected by the presence of Tat. Furthermore, the methylation specific PCR analysis of the stably Tat expressing cell lines revealed that the CD99 promoter is de methylated in the presence of Tat. Taken together, these results open a potential role of CD99 in AIDS-related oncogenesis via epigenetic regulation by HIV-1 Tat.

• Korean Journal of Food Science and Technology
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• v.46 no.4
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• pp.438-445
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• 2014

We examined biogenic amine (BA) production as well as the diversity of bacterial flora in 11 types of commercial makgeolli stored at 4 and $20^{\circ}C$. Moreover, we studied the BA-producing activity of three L. plantarum strains isolated from makgeolli. At $20^{\circ}C$, the BA content was highly increased and the denatured DNA bands were more variable in non-sterilized makgeolli compared to sterilized makgeolli. The major BAs produced in commercial makgeolli were histamine and putrescine. Histamine, tyramine, putrescine, and cadaverine were produced in excess by inoculation of the three L. plantarum isolates to makgeolli stored at $20^{\circ}C$ for 21 days. These results suggest that some L. plantarum strains in makgeolli can produce different types of BAs, depending on the extent of degradation of makgeolli.

• Korean Journal of Heritage: History & Science
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• v.37
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• pp.309-326
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• 2004

There are some methods such as fatty acid analysis and microscope analysis of parasite egg and pollen and genetic analysis of ancient bacteria from ancient soil. The fatty acid analysis can examine whether some materials is human feces or animals. This is important thing to reconstruct ancient toilet culture pattern. The methods using TLC and GC-MS as organic chemistry is able to confirm ancient diet life style and nutritive conditions. The microscope analysis of ancient soil is able to confirm ancient parasite egg and pollen. It is possible to analogize ancient human diseases from this analysis. Also, genetic analysis is able to confirm genetic diversity and variation pattern of ancient organisms in archeological soil. Most of all, it is convinced of carrying through genetic preservation of exterminated ancient organisms. If archeological soils should be analysed through the natural scientific methods such as organic chemistry, soil science, microbiology, molecular biology, and genetics, this is helpful for us to understand and interpretation past historic event. And it is expected to perform an major role for understanding origin of ancient human and life style.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.117-124
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• 2016

Researches on soil microbial community are increasing to assess ecosystem responses to anthropogenic disturbances and to provide an indicator of ecosystem recovery. Microbial communities are able to respond more rapidly to environmental changes than plants and therefore they may provide an early indication of the ecosystem recovery trajectory. This study was conducted using 16S rRNA gene pyrosequencing of soil samples to compare soil bacterial community composition between artificially covered soils of the Baedudaegan ridgeline and their adjacent forest soils in two restoration project sites, Ihwaryeong and Yuksimnyeong, which were completed in 2012 and 2013, respectively. Richness of the Phylum level was 29.3 in Ihwaryeong and 32.3 in Yuksimnyeong. Significant difference in the richness between artificial restored soils and adjacent forest soils(p<0.01) was observed, however no significant difference was observed for site location and soil depth. Acidobacteria(37.3%) and Proteobacteria(31.1%) were more abundant than any other phylum in collected soil samples. Also, we found the significant difference in the relative abundance of the two abundant phyla between artificially restored soils and their adjacent forest soils (Proteobacteria, 38.1% in restored soils vs 24.2% in adjacent forest soils, p<0.01; Acidobacteria, 55.4% in restored soils vs 19.2% in adjacent forest soils, p<0.001). The results support the previous researches indicating that soil bacterial community composition is affected by nutritional status of soils and that Acidobacteria is also strongly influenced by pH, thus favoring soils with lower pH. This study could be utilized to monitor and evaluate restoration success of forest soil environment quantitatively.

• Journal of the Korean Society for Marine Environment & Energy
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• v.20 no.1
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• pp.1-11
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• 2017

Nakdong River Estuary is affected by the dam, barrage construction and dredge and reclaim worked artificially. So, we have studied the area input both freshwater and sea water to understand marine environment of Nakdong River estuary related to the effect of artificial work from 2013 to 2015. As a result, The discharge flow to the estuary remarkably decreased before barrage construction and the average of salinity at the estuary increased. So, the brackish water zone reduced under the influence of decreased discharge flow. The major sources of nitrate and silicate were freshwater, phosphate supplied from bottom and the open sea water. The concentration of phosphate and dissolved oxygen (DO) decreased remarkably in spring and summer. we investigated that phosphate in freshwater was removed under the influence of the estuary dam and phosphate in sea water was removed under the influence of phytoplankton. The low concentration of DO was due to decomposition of the organic compound by microorganism after phytoplankton blooms. Generally, the concentrations of chlorophyll-a in summer was higher than spring and fall. Therefore, the change of ecosystem in Nakdong river estuary was due to decrease of freshwater influx, the other change is facing because of the barrage.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.650-658
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• 2010

We used green manure crops such as hairy vetch, crimson clover, rye, sorghum, and sudan grass by mixing with soils to assess the effects of green manure crops on nutrient supply and soil quality improvement. Temporal changes in soil inorganic nitrogen, carbohydrate, microbial biomass, and humus content were determined as soil quality indicators. Inorganic nitrogen content of the control maintained similar level during the whole period, but it had continually increased until 4 weeks after incorporation (WAI) of green manure crops. Especially, inorganic nitrogen content sharply increased in sudan grass. After incorporation of green manure crops, temporal change of soluble sugar in soils was as follows: it had gradually increased in legume green manure crops-incorporated soils until 7 WAI, which was the highest, and then showed the tendency to be reduced. Meanwhile, it in non-legume green manure crops-incorporated soils rapidly increased after the incorporation, and reached the maximum around 4 WAI. Humic acid by the decomposition of crop residues in green manure crops-incorporated soils was greatly enhanced with the elapsed time of 4 WAI, although it was low at the same level as the control until 2 weeks. In addition, there was a difference in fulvic acid by incorporated crops, fulvic acid in hairy vetch, sorghum and sudan grass showed a similar tendency with the change in humic acid. Our results suggest that soluble sugar, microbial activity and humic acid could be available indicators to evaluate the fertility of green manure crops-incorporated soils.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.136-141
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• 1997

In this study, we can be obtained the following conclusions about the characteristics of leachate with passed time in the expired landfill. Compared with the expired landfilling H. and S., pH shows a tendency to increase in E., because $NH_3$ is created by anaerobic degradation and dissolved in leachate. The initial BOD and COD of E. and S. are high in similar concentration but H. is low, Since the organics of waste in H. are lower than in E. and S. during landfilling. SS of E. in concentration is increased and very altered, because food of combustible in E. is higher than it in H. and S.. According to passed time, T-N is high in concentration but T-P shows a similar tendency. Heavy metals of leachate is lower than threshold concentrations. If leachate is treated biologically, microbes are not inibitory.

• Journal of Korea Foresty Energy
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• v.24 no.2
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• pp.1-9
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• 2005

After manufacturing fermentation system for degrading pig manure using environmentally friendly technique, performance of the system and characteristics of wood chips and pig manure fermented in the system were analyzed. Results from this study shows that proper fermentation temperature($55{\sim}60^{\circ}C$) reached 3days after the system started and degradation rate, which expresses fermentation performance of system, was $180{\iota}$/day. Even as progressing the fermentation of wood chips and pig manure mixture, the amount of extractives drawn out by alkali, and alcohol-benzene and lignin content was not varied. However, ash content in wood was increased. The inorganic compounds in pig manure seem to be transferred into wood chip. On the other hand holocellulose contents in wood were decreased a little. Holocellulose seems to be consumed as the second carbon source in fermentation process. Results through analysis of inorganic- and heavy metal elements contents in wood chips and pig manure fermented in long term process shows that inorganic elements($Ca^{2+},\;Mg^{2+},\;K^+,\;Na^+$ etc.) contents were increased with fermentation time and heavy metal elements(Cd, As, Cu etc.) which cause environmental pollution were not detected. Number of microorganisms including bacteria, actinomycetes, and fungi, the number of C.F.U(Colony Forming Unit) was increased while temperature in fermentation system was abruptly increased.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.9
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• pp.1202-1207
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• 2008

The study examined changes in the quality characteristics of Aralia continentalis Kitagawa (AcK) leaf-kimchi during storage prepared with different seasonings. The result exhibited practically no significant differences in pH, acidity, and salinity among the various samples made with three different combinations of seasonings. In AcK leaf-kimchi, lightness and redness increased slowly at the early period of storage and then decreased rapidly after four months when the lactic acid bacterial count reached the highest level with fermentation, whereas yellowness increased. The total bacterial count and the lactic acid bacterial count increased slowly reaching the highest level after four months, then decreased and maintained at a constant level. Hardness of AcK continuously increased and then decreased after attaining maximum value during ripening. The sensory characteristics of the AcK in the whole period of storage was highly acceptable in terms of color, smell, bitterness, texture, and general preference regardless of the suggested combinations of seasonings.