• Journal of Life Science
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• v.23 no.6
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• pp.751-756
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• 2013

This research old age chestnut tree that loss function chestnut leaf renewal efficiency enlargement and development of cosmeceutical product by purpose. Chestnut leaf (CL) exhibits numerous pharmacological effect including anti-allergy and anti-microbial properties. However, the anti-wrinkle effect of CL is not completely understood. In this study, to find a possible explanation for the anti-wrinkle effects of CL, we evaluated the effects of CL on radical scavenging activity, elastase inhibition activity and collagenase inhibition activity. CL was extracted with various solvents including water (CLW), 70% ethanol (CLE) and 70% acetone (CLA). The results showed that CLW, CLE, and CLA have the radical scavenging activity. In addition, we showed that CLW, CLE, and CLA have the elastase inhibition activity and collagenase inhibition activity. Consequently, the CL has a potent anti-wrinkle effect and it could be a useful cosmeceutical product for anti-aging purposes.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.189.2-190
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• 2003

현재까지 미생물의 증식을 억제하는 보존제로는 인공합성품이 많이 알려져 이들이 주로 상업적으로 사용되고 있으나 그 안정성이 경우에 따라 문제되고 있으며 근래소비자의 건강 지향적 욕구가 증대됨에 따라 인공합성품의 기피현상이 두드러지고 있다. 따라서 이들 천연 항균물질의 개발과 이용은 인공 합성보존제의 대처라는 의미와 소비자기피현상을 유발시키지 않으면서 각종 가공식품의 저장성향상 및 저온 유통식품의 안정성확보라는 견지에서 그 중요성이 있다. 본 실험에서는 식품의 미생물 작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(Botanical antimicrobial agents-Citrus product : 이하 BAAC라 칭함)에 천연보조제(Ginseng extract, Aloe, 매실추출물)를 첨가하여 Paper disk 법에 의한 항균성 검사를 실시한 결과 Ginseng extract를 첨가한 경우 생육저해환이 가장 크고 뚜렷하게 나타났다. 따라서 BAAC에 보조제(Ginseng extract)를 첨가하여 제조 된 천연 항균복합제재(이하 BAAG라 칭함)를 우유에는 각 농도별로 첨가하여 4$^{\circ}C$의 냉장상태와 2$0^{\circ}C$의 상온에서 저장하였고, 계란과 콩나물의 경우는 침지 처리한 후 꺼내어 상온에서 저장하면서 각각 주사전자현미경 관찰, 대장균수, 총균수의 변화, 외관상의 변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 아울러, 계란의 경우는 조단백함량의 변화도 관찰하였다. 주사전자현미경 촬영사진의 결과 분석을 통하여 BAAG를 처리한 균체 세포는 세포막 및 세포벽 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되며 성장을 저해 또는 사멸시키는 것으로 나타났다. 대장균수는 우유, 달걀 및 콩나물의 모든 처리구에서 BAAG의 첨가에 의해 성장이 억제되었으며 농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였으며, 총균수도 압도적으로 낮은 간을 보여주었다. 달걀의 조단백함량의 변화는 BAAG를 처리하지 않은 무처리 대조구에 비하여 침지 처리한 경우가 조단백 변화가 크지 않았다. 즉, BAAG의 침지 처리한 경우, 저장기간 14일이 경과 한 후에 10%정도의 수준으로 감소한데 반하여, 무처리구인 대조구의 경우, 23%수준으로 감소 증도가 증가하였다. 외관상의 변화와 상품 가치를 측정한 결과는 달걀의 경우, 14일이 경과후에는 무처리 대조구에서 흰자위의 감소와 노른자위와의 경계가 뚜렷하지 못하여 상품으로서의 가치가 크게 떨어진 상태였다. 우유의 경우에는 4$^{\circ}C$와 2$0^{\circ}C$에 저장된 대조구에서는 각각 3일과 12일경과 후 강한 부패취와 아울러, 표피의 갈변정도가 심하되어 관능적으로 부패상태를 인지할 수 있었다. 콩나물의 경우도 저장3일 경과 후에 부패취와 함께 점질성 갈변물질이 생성되었다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 농산물 식품원료에 적용하여 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.4
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• pp.265-270
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• 2014

Protease producing microorganisms were isolated from many kinds of food waste and fermented foods, which contains high amount and variable kinds of degraded substances. Several microorganisms were identified by 16S rRNA full sequencing analysis methods. The activity of protease was analyzed and identified in variable conditions for the application. For industrial use for biowaste treatment some proteases were isolated, identified and selected from microbial cells. And the tests were carried for the further use. The protein degrading activity at low temperature is useful for the treatment of organic waste, which contains much proteins. By the protein degradation process the organic waste can be utilized in variable fields, for example from feedstuff supplement to fertilizer for agriculture. Bacterial cells with protease activity at low temperature were isolated and identified. The optimal conditions for microbial cultivation and protease production were studied.

• Resources Recycling
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• v.18 no.4
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• pp.70-81
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• 2009

The patents were searched to investigate the trend and the direction of technologies about the recycling of WEEE (waste electric and electronic equipment). Database was collected from WIPS site, and then 223 patents were selected by investigating abstracts. The patents from 1986 to 2007 were analyzed according to countries, international patent classification (IPC), companies, technologies, and recycling-target materials. The most patents were applied in Japan and by Japan companies. Patents about physical pretreatment technology are the most among the patents of the WEEE recycling technologies. Patents about printed circuit boards among target materials are the most because of its high value-added.

• Resources Recycling
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• v.16 no.6
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• pp.28-33
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• 2007

Coal is the most abundant energy source and deposited in every area of world. Combustion process with lower efficiency has been mainly used. Therefore, implementation of more efficient technologies, involving gasification, combined cycles and fuel cells, would be a key issue in the plans for more efficient power generation. In these technologies, gasification has been studied for decades. However, coal gasification to high value combustible gas such as hydrogen and carbon monoxide is focused again due to high oil price. The gaseous product, called syngas, can be effectively utilized in a variety of ways ranging from electricity production to chemical industry (as feedstock). In this study, coal gasification with ultra high temperature steam has been performed. The effect of steam/carbon ratio on the produced gas concentrations, gasification rate and additional products like tar, ammonia and cyan compounds has been determined.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.2
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• pp.129-134
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• 2016

Odor in pig manure affects the distribution of the manure over grass and crop fields as fertilizer. The objective of this study was to investigate the effect of different types of microbes (Saccharomyces cerevisiae, Bacillus subtilis and Rodobacter capsulata) and incubation temperatures ($20^{\circ}C$ and $35^{\circ}C$) on the levels of odorous compounds in pig manure. Pig manure was incubated with 0.03% microbes (v/v) at temperatures of $20^{\circ}C$ or $35^{\circ}C$. At incubation temperature of $20^{\circ}C$, the addition of Rodobacter capsulata significantly (p<0.05) decreased the levels of indoles and volatile fatty acid (VFA). At incubation temperature of $35^{\circ}C$, the addition of any microbes of the three used in this study did not significantly (p>0.05) affect the levels of odorous compounds. When incubation temperature was increased from $20^{\circ}C$ to $35^{\circ}C$, levels of odorous compounds were significantly (p<0.05) increased. Taken together, these results suggest that Rodobacter capsulata could be utilized to reduce odor from pig manure in the spring and fall when the average temperature is around $20^{\circ}C$. However, alternative odor-reducing technology is needed to be developed to apply onto pig manure during the hot summer season ($35^{\circ}C$).

• Journal of Life Science
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• v.31 no.9
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• pp.862-872
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• 2021

Recent studies on synthesis of metallic nanomaterials such as silver (Ag), gold (Au), platinum (Pt), cerium (Ce), zinc (Zn), and copper (Cu) nanoparticles (NPs) using plants and microbes are attracted researchers for their wide range of applications in the field of biomedical sciences. The plant contains abundant of bioactive contents such as flavonoids, alkaloids, saponins, steroids tannins and nutritionals components. Similarly, microbes produce bioactive metabolites, proteins and secretes valuable chemicals such as color pigments, antibiotics, and acids. Recently reported, biogenic synthesis of NPs in non-hazardous way and are promising candidates for biomedical applications such as antibacterial, antifungal, anti-cell proliferative and anti-plasmodia activity. All those activities are dose dependent, along with their shape and size also matters on potential of NPs. Microbes and plants are great source of metabolites, those useful in biomedical field, such metabolites or chemicals involved in synthesis of NPs in an ecofriendly way. NPs synthesized using microbes or plant materials are reveals more non-toxic, facile, and cost-effective compare to chemically synthesized NPs. In present review we are focusing on NPs synthesis using biological agents such as microbes (bacteria, fungi and algae) and plant, characterization using different techniques and their antibacterial applications on pathogenic Gram-positive and Gram-negative organisms.

• Journal of the Korean Geographical Society
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• v.31 no.4
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• pp.685-698
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• 1996

Southern Louisiana has one of the largest Vitnamese refrgee neighborhoods after the mid-1970s. It is impressive that one of their adaptive strategies comes from their religious lives which are centered on either Catholicism or Buddhism. The Buddhism community, especially, exhibits an exotic symbolic system of value and attitude, and thus contributes to cultural diversity in the adopted country. The landscape of the Buddhist temple is a visible symbol to them that the host socirty accepts their maintenance of their own cultural identity and that they are also an integral part of American society. Their making-place and being-in-place procedures, although their culture is being transformed in the original shape, put an emphasis on interaction with the host xociety. These procedures have been facilitated by consolidating their identity as a minority group as well as by interacting with the host society. The on-going influx of foreign immigrant groups seems not to drive them to assimilate into the melting-pot society, but to contribute to contribute to the increase in the cultural diversity of the United States.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.1
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• pp.1-7
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• 2016

The aim of this study was to evaluate the cytotoxicity of cadmium chloride ($CdCl_2$), toxicant, and the protective effect of Chelidonium majus (CM) extract on $CdCl_2$-induced cytotoxicity in cultured NIH3T3 fibroblasts. Cell viability, the effect of butylated hydroxytoluene (BHT) against $CdCl_2$, and the antioxidative effects including DPPH-free radical scavenging activity, superoxide anion-radical scavenging activity (SSA), and lactate dehydrogenase (LDH) activity were assessed. $CdCl_2$ caused a significant dose-dependent decrease in cell viability, and $XTT_{50}$ value was determined at 38.7uM of $CdCl_2$. It was determined as highly-toxic by Borenfreund and Puerner' toxic criteria. BHT of antioxidant significantly increased cell viability severely damaged by $CdCl_2$-induced cytotoxicity in these cultures. In the protective effect of CM extract on $CdCl_2$-induced cytotoxicity, CM extract significantly increased cell viability, DPPH-free radical scavenging activity, SSA and inhibitory activity of LDH. From these results, it is suggested that oxidative stress is involved in the cytotoxicity of $CdCl_2$, and CM extract showed protective efficacy on $CdCl_2$-induced cytotoxicity via antioxidative effects. Conclusively, natural resources like CM extract may be a putative antioxidative agent for the detoxification or diminution of toxicity correlated with oxidative stress.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1073-1077
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• 2006

급속한 산업화로 인한 오염물질의 증가와 생물서식처의 감소는 수자원과 생태계를 위협하고 있다. 국내의 경우 수자원의 질을 개선하기 위해 '90년대 초부터 하수처리시설 등의 저감시설을 대폭 확충하였으나 현재까지 팔당호 등 주요상수원이 목표수질에 못 미치고 있으며 그 원인은 유입오염물질의 $22{\sim}37%$를 차지하는 비점오염원으로 지목되고 있다. 또한 생태.경관적 가치가 높은 수변지역은 각종 개발로 생물서식처가 급속도로 감소하여 종 다양성 보전 측면에서 대책마련이 시급한 실정이다. 이와 유사한 상황에 직면한 선진외국에서는 '하천회랑(river corridor)' 또는 '토양 및 생태시스템을 포함하는 수역과 육역의 점이(漸移)지대'를 의미하는 이른바 '수변완충지대(Riparian Buffer Zones)'의 오염정화 및 생태조성 효과 등의 연구를 통해 효율적 조성방안을 제시하고 있으며 다양한 형태로 현장에 적용하고 있다. RBZs의 일반적인 기능으로는, 유사나 오염물질의 여과 및 차단(필터링 효과), 영양염류의 저감, 하천변 식생을 통한 수자원의 정화 및 강턱의 안정화, 홍수로 인한 하천침식의 방지, 수변 생물 서식처 제공, 수변 그늘 제공에 의한 수온상승 방지, 심미 교육 위락 공간 제공 등이다. 본 연구에서는 외국의 RBZs(Riparian Buffer Zones)가이드라인을 참고하여 국내실정에 맞는 파일럿 규모의 시험완충지를 설계 및 조성하였다. 시험완충지는 남한강 연안에 초본류, 갈대류, 관목류, 자연식생, 혼합식생 등 5가지 'dry biotope'형태로 설치하여 1년간 계절별로 운영하였다. 또한 실험의 정량화와 다양한 조건변화를 위해 차수막, 위어, 유량.농도 조절장치, 라이시미터 등 보조시설을 설치하였고, 정기적인 모니터링을 실시하였다. 조사결과 외국사례를 살펴보면 RBZs의 적정 폭은 수질정화기능의 경우 $15{\sim}30m$, 생태서식처 기능은 최소 90m이상으로 제시되며, 시험완충지의 수질정화효과는 SS, T-N, T-P, TOC의 평균저감율이 각각 50%이상으로 나타났다. 식생모니터링 결과, 환삼덩굴 등 우점종의 잠식속도는 약 15일이며 갈대와 갯버들의 경우 우기시 인공목책호안과 동일한 침식방지 효과를 보이는 것으로 관찰되어 식생의 주기적인 모니터링과 지역 특성에 적합한 우점종 선정이 매우 중요한 것으로 판단된다.