• Title/Summary/Keyword: 문자 분할

Search Result 218, Processing Time 0.028 seconds

Purification and Characterization of the Bacteriocin Produced by Lactococcus sp. KD 28 Isolated from Kimchi (김치에서 분리한 Lactococcus lactis가 생산하는 박테리오신의 정제 및 특성)

  • Lee, Ji-Young;Choi, Nack-Shick;Chun, Sung-Sik;Moon, Ja-Young;Kang, Dae-Ook
    • Journal of Life Science
    • /
    • v.25 no.2
    • /
    • pp.180-188
    • /
    • 2015
  • The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.

A study on content strategy for long-term exposure of YouTube's 'Trending' (유튜브 '인기급상승' 장기 노출을 위한 콘텐츠 전략에 관한 연구)

  • Lee, Min-Young;Byun, Guk-Do;Choi, Sang-Hyun
    • Journal of the Korea Convergence Society
    • /
    • v.13 no.4
    • /
    • pp.359-372
    • /
    • 2022
  • This study aimed to derive a YouTube content strategy that can be exposed to Trending for a long time by comparing the features of 20 channels in the short/long term using 'YouTube Trending' data in 2021. First, through Pearson's correlation analysis, we found that various factors such as 'the number of title or tag letters' related to long-term exposure, and set this as an index to compare features. As a result, 1)'video title' of about 40-45 letters without excessive special characters, 2)'video length' within 10 minutes, 3)'Video description' is effective when writing 2-3 sentences and adding SNS information or including 3 key tags. Also, it would be more effective if you set key tag pairs such as (먹방, mukbang), (역대급, 레전드) derived through text mining. Through this, the channel will spread globally, bringing various advantages, and will be used as an indicator to evaluate the globality of the channel.

Effect of Water Quantity in Pot on Growth of Some Wood Plant by Water Flooding Culture (몇 가지 목본식물의 담수 재배 시 용기 내 관수량이 식물의 생육에 미치는 영향)

  • Song, C.Y.;Moon, J.Y.
    • Journal of Practical Agriculture & Fisheries Research
    • /
    • v.21 no.2
    • /
    • pp.49-58
    • /
    • 2019
  • This study aimed to determine of water quantity in pot for water flooding culture of Pinus thunbergii Parl., Chaenomeles japonica Lindl. ex Spach and Osmanthus fragrans Lour. The survival rate of P. thunbergii Parl. and C. japonica Lindl. ex Spach in the water quantity in pot 60% or 40% was 100% and the rate of O. fragrans Lour. in the 60% or 40% water was 90.0% or 93.3%. However the survival rate of the 100% water was less than 90% in P. thunbergii Parl, C. japonica Lindl. ex Spach and less than 60% was in O. fragrans Lour. The increasing rate of plant height for P. thunbergii Parl. in the water quantity in pot 40% or 60% was above 50%, and the C. japonica Lindl. ex Spach or O. fragrans Lour. was above 90%. However the increasing rate of plant height in the water 100% was less then the others as a 38.2%, 65.4% or 66.7% in respectively in P. thunbergii Parl., C. japonica Lindl. ex Spach and O. fragrans Lour. The increasing rate for leaf number for P. thunbergii Parl. in the water quantity 40% or 60% was above 80%, and the C. japonica Lindl. ex Spach or O. fragrans Lour. was above 70%, however the 100% water was below 60% in all treatment. Therefore, the survival rates in some woody plants of the 40% or 60% water in the pot was above 90% and the plant growth of plant height, plant width, leaf number or fresh weight was proper increased. But the survival rates and growth of the 100% water in the pot was decreased

Effects of Light Quality, Temperature or Vermiculite Depth on Germination for the Veronica L. (광질, 온도 및 복토 깊이가 꼬리풀 (Veronica L.) 종자의 발아에 미치는 영향)

  • Cheon Young Song;Ja Young Moon;Jung Won Sung;Byeong Seon Park;Jae Ik Nam;Jeong Min Kim
    • Journal of Practical Agriculture & Fisheries Research
    • /
    • v.25 no.1
    • /
    • pp.36-47
    • /
    • 2023
  • This study was conducted to develop a mass production for a commercial use by germination of 4 kinds of Veronica glabrifolia Kitag., V. pusanensis Y.N.Lee, V. glabrifolia Kitag. × V. Spicata 'Alba' and V. spicata 'Ulster Blue Dwarf × V. longifolia. Veronica L. Effects of dipping time to a disinfecting fluid, light quality, temperature or vermiculite depth on the germination of Veronica L. were examined. Germination percentage in a disinfecting fluid for 120 minutes dipping was the 75.1% for V. pusanensis Y.N.Lee, and was some higher in 60 minutes dipping for all the species, but there was no significant difference according to the dipping time. The germination of the light quality was the best in complex light (red light + blue light) as the germination 92.8%, uniformity 2.3 in Veronica glabrifolia Kitag., or the germination 85.9%, uniformity 3.5 in V. pusanensis Y.N.Lee. And the germination percentage of red light was ranged from 79.1% to 95,5%, the blue light was 83.1% to 100% in the all species. However the germination in the dark condition significantly lowered as a 40.0% to 56.3% or uniformity from 5.2 to 6.5. Like this, the germination of blue light was better compare to the red light. The germination rate according to temperature was higher at 25℃ day/20℃ night ranged from 92.1% to 100%, or at 20℃ day/15℃ night as a 85.5% to 98.9% in the all species, compare to constant temperature 25℃ as a 80.9% in V. pusanensis Y.N.Lee. The germination rate of vermiculite depth was higher in 1 to 2mm ranged from 83.4% to 100%, however when the vermiculite was covered with 4mm, the germination rate significantly decreased from 45.2% to 72.1% and the hypocotyl length became longer above 3cm than that of others.

Effect of Quartz Porphyry and Hydroball Ratio on Growth of Some Wood Plant by Water Flooding Culture in Pot (목본식물의 분 담수 재배에서 맥반석 및 하이드로 볼의 비율이 식물의 생장에 미치는 영향)

  • Song, C.Y.;Moon, J.Y.
    • Journal of Practical Agriculture & Fisheries Research
    • /
    • v.21 no.1
    • /
    • pp.15-28
    • /
    • 2019
  • This study aimed to select the media for water flooding culture of Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, Osmanthus fragrans Lour., and Chaenomeles japonica Lindl. ex Spach planting into quartz Porphyry and Hydroball. The survival rate of Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach in hydroball 80%+quartz porphyry 20% was 100%, also the ratio of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour. in hydroball 20%+quartz porphyry 80% was 100% and 80%. However survival rate of full water and decomposed granite was less than 80% in Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, and Chaenomeles japonica Lindl. ex Spach and thee rate of Osmanthus fragrans Lour was less than 50%. The increasing rate of fresh weight for Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach in hydroball 80%+quartz porphyry 20% was 58.6% and 15.8% which was higher than others, and the fresh weight was increased as increasing the content of hydrobol. However the fresh weight of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour. in hydroball 20%+quartz porphyry 80% was 71.4% and 59.7% which was higher than others, and the fresh weight was increased as increasing the content of quartz porphyry. The increasing rate of fresh weight of decomposed granite as control was the lowest by 32.7%, 48.0%, 33.3% and 7.0%, respectively in Pinus thunbergii Parl., Juniperus chinensis L. var. sargentii Henry, Osmanthus fragrans Lour., and Chaenomeles japonica Lindl. ex Spach. Therefore the survival rate and fresh weight was lower at water and decomposed granite. However the survival rate and increasing fresh weight of Pinus thunbergii Parl. and Chaenomeles japonica Lindl. ex Spach was higher as increasing the rate of hydrobol. And the survival rate and fresh weight of Juniperus chinensis L. var. sargentii Henry and Osmanthus fragrans Lour was higher as increasing the rate of quartz porphyry.

Characterization of Bacteriocin Produced from Isolated Strain of Bacillus sp. (Bacillus 속 분리주가 생산하는 박테리오신의 특성 조사)

  • Ham, Seung-Hee;Choi, Nack-Shick;Moon, Ja-Young;Baek, Sun-Hwa;Lee, Song-Min;Kang, Dae-Ook
    • Journal of Life Science
    • /
    • v.27 no.2
    • /
    • pp.202-210
    • /
    • 2017
  • As an effort to find a potential biopreservative, we isolated bacterial strains producing bacteriocin from fermented foods. A strain was finally selected and characteristics of the bacteriocin were investigated. The selected strain was identified as Bacillus subtilis E9-1 based on the 16S rRNA gene analysis. The culture supernatant of B. subtilis E9-1 showed antimicrobial activity against Gram-positive bacteria. Subtilisin A, ${\alpha}$-chymotrypsin, trypsin and proteinase K inactivated the antimicrobial activity, which means its proteinaceous nature, a bacteriocin. The bacteriocin activity was fully retained at the pH range from 2.0 to 8.0 and stable at up to $100^{\circ}C$ for 60 min. Solvents such as ethanol, isopropanol and methanol had no effect on the antimicrobial activity at the concentration of 100% but acetone and acetonitrile reduced the activity at up to 100% concentration. Cell growth of four indicator strains was dramatically decreased in dose-dependent manner. Listeria monocytogenes was the most sensitive, but Enterococcus faecium was the most resistant. Bacillus cereus and Staphylococcus aureus showed the medium sensitivity. The bacteriocin showed its antimicrobial activity against B. cereus and L. monocytogenes via bactericidal action. The number of viable cells of L. monocytogenes started to reduce after addition of bacteriocin to the minced beef. The bacteriocin was purified through acetone concentration, gel filtration chromatography and RP-HPLC. The whole purification step led to a 6.82 fold increase in the specific activity and 6% yield of bacteriocin activity. The molecular weight of the purified bacteriocin was determined to be 3.3 kDa by MALDI-TOF/TOF mass spectrometry.

Correlation of serum total hydroperoxide levels and diseases of prematurity (미숙아 합병증 발생과 혈중 활성 산화물 농도와의 관계)

  • Kim, Shin-Hye;Hur, Hae Young;Lee, Kyu Hyoung;Moon, Ja Young;Chae, Kyu Young
    • Clinical and Experimental Pediatrics
    • /
    • v.50 no.8
    • /
    • pp.746-752
    • /
    • 2007
  • Purpose : Total hydroperoxide (TH), free radical-mediated oxidation product can be used as a measure of free radical injury. The aim of the present study was to see if preterm newborns are at increased risk for oxidative stress compared with term newborns, and to determine whether oxidative stress during postnatal first 1 week is associated with clinical outcomes in preterm infants. Methods : Serum TH levels of preterm infants (n=39) were compared with those of term infants (n=24) on the postnatal day 1. Among the preterm infants, serum TH levels of uncomplicated group (n=23) were also compared with those of complicated group (n=16) who developed oxygen radical related diseases on the postnatal day 1 and 7. Retrospective analysis was performed to find out risk factors for oxygen radical injuries based on birth history, laboratory data, neuroimaging findings and clinical progress in two preterm groups. Results : Serum TH levels on postnatal day 1 were higher in the preterm infant group than the term infant group. Serum TH levels on postnatal day 1 in the complicated preterm infant group were significantly higher compared with uncomplicated group, but there was no significant difference in serum TH levels on postnatal day 7. Also, there was no significant difference in serum TH levels between uncomplicated preterm infants and term infants. Serum TH level on postnatal day 1 was independently associated with higher morbidity after adjusting for gestational age, Apgar score (5 min), arterial blood gas analysis. Conclusion : Complicated preterm newborns are at increased risk for oxidative stress compared with uncomplicated newborns and term newborns. Oxidative injury during the prenatal or postnatal day 1 is associated with adverse outcomes in preterm infants. Elevated TH levels on postnatal day 1 may have a value to predict clinical outcomes in preterm infants.

Documentation of Intangible Cultural Heritage Using Motion Capture Technology Focusing on the documentation of Seungmu, Salpuri and Taepyeongmu (부록 3. 모션캡쳐를 이용한 무형문화재의 기록작성 - 국가지정 중요무형문화재 승무·살풀이·태평무를 중심으로 -)

  • Park, Weonmo;Go, Jungil;Kim, Yongsuk
    • Korean Journal of Heritage: History & Science
    • /
    • v.39
    • /
    • pp.351-378
    • /
    • 2006
  • With the development of media, the methods for the documentation of intangible cultural heritage have been also developed and diversified. As well as the previous analogue ways of documentation, the have been recently applying new multi-media technologies focusing on digital pictures, sound sources, movies, etc. Among the new technologies, the documentation of intangible cultural heritage using the method of 'Motion Capture' has proved itself prominent especially in the fields that require three-dimensional documentation such as dances and performances. Motion Capture refers to the documentation technology which records the signals of the time varing positions derived from the sensors equipped on the surface of an object. It converts the signals from the sensors into digital data which can be plotted as points on the virtual coordinates of the computer and records the movement of the points during a certain period of time, as the object moves. It produces scientific data for the preservation of intangible cultural heritage, by displaying digital data which represents the virtual motion of a holder of an intangible cultural heritage. National Research Institute of Cultural Properties (NRICP) has been working on for the development of new documentation method for the Important Intangible Cultural Heritage designated by Korean government. This is to be done using 'motion capture' equipments which are also widely used for the computer graphics in movie or game industries. This project is designed to apply the motion capture technology for 3 years- from 2005 to 2007 - for 11 performances from 7 traditional dances of which body gestures have considerable values among the Important Intangible Cultural Heritage performances. This is to be supported by lottery funds. In 2005, the first year of the project, accumulated were data of single dances, such as Seungmu (monk's dance), Salpuri(a solo dance for spiritual cleansing dance), Taepyeongmu (dance of peace), which are relatively easy in terms of performing skills. In 2006, group dances, such as Jinju Geommu (Jinju sword dance), Seungjeonmu (dance for victory), Cheoyongmu (dance of Lord Cheoyong), etc., will be documented. In the last year of the project, 2007, education programme for comparative studies, analysis and transmission of intangible cultural heritage and three-dimensional contents for public service will be devised, based on the accumulated data, as well as the documentation of Hakyeonhwadae Habseolmu (crane dance combined with the lotus blossom dance). By describing the processes and results of motion capture documentation of Salpuri dance (Lee Mae-bang), Taepyeongmu (Kang seon-young) and Seungmu (Lee Mae-bang, Lee Ae-ju and Jung Jae-man) conducted in 2005, this report introduces a new approach for the documentation of intangible cultural heritage. During the first year of the project, two questions have been raised. First, how can we capture motions of a holder (dancer) without cutoffs during quite a long performance? After many times of tests, the motion capture system proved itself stable with continuous results. Second, how can we reproduce the accurate motion without the re-targeting process? The project re-created the most accurate motion of the dancer's gestures, applying the new technology to drew out the shape of the dancers's body digital data before the motion capture process for the first time in Korea. The accurate three-dimensional body models for four holders obtained by the body scanning enhanced the accuracy of the motion capture of the dance.