• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.11-19
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• 1984

Potted, germinating Pinus rigida ${\times}$ P. taeda seedlings were inoculated with Pisolithus tinctorius (Pt) ectomycorrhizal fungus to test the effectiveness of Pt in relation to organic amendment and changes in soil fertility and soil texture. Pt was cultured as mycelia in vermiculite-peat moss mixture with nutrients and added to sterilized pot soils with or without organic amendment (fully fermented compost) at three soil texture levels (sand, loamy sand, and sandy loam) in a factorial design. Plants were grown in a greenhouse for 4 months and harvested to compare their growth with non-mycorrhizal plants and plants infected by natural fungi. Regardless of sod texture, soil fertility, or organic amendment, seedlings inoculated with Pt were better in dry weight and height than non-mycorrhizal plants or those infected by natural fungi. An exception was observed in the most fertile soil (0.075% N and 1.32% organic matter content in sandy loam with organic amendment), where non-mycorrhizal plants were slightly bigger (8%) and heavier (18%) than Pt-inoculated plants. In over-all average, Pt-inoculated seedlings were 30% taller and 107% heavier than those infected by natural fungi and 31 % taller and 60% heavier than non-mycorrhizal plants. Growth stimulation of seedlings by Pt was more pronounced in less fertile sand soil when organic was not amended. Mycorrhizal frequency of Pt (% of mycorrhizal root tips) was reduced to about half (from 84 to 33% in sandy loam and from 77 to 40% in loamy sand) by organic amendment, while that of natural fungi was not significantly affected. Severe nitrogen deficiency was observed in the needles of non-mycorrhizal plants (1.38% N), while both Pt-inoculated plants (1.68% N) and those infected by natural fungi (1.89% N) did not develop symptom, suggesting an active role of mycorrhizae in absorption of soil nitrogen. Top to root ratio increased with organic amendment to non-mycorrhizal plants, but was not significantly affected by fungal treatment. It was concluded from this study that relative effectiveness of Pt was determined by soil fertility. Organic amendment to less fertile sand soil increased effectiveness of Pt, while the same amendment to more fertile loamy sand and sandy loam decreased effectiveness of Pt. Benefits of Pt mycorrhizae would be expected most either when organic was not added to the soil, or when soil nutrients were not abundant.

• Journal of Environmental Science International
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• v.8 no.2
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• pp.165-169
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• 1999

The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6.25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing antibody test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.6
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• pp.1094-1099
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• 1998

Newly introduced aseptic process for cooked rice was optimized by evaluating the process parameters. The optimal conditions were as follows: ratio of washing water to rice, 3:1 by weight; steeping at 20oC for 20min; heat shock at 140oC for 7sec with 8 repetitions; addition of 0.2% glucono lactone solution; cooking at 103oC for 30min. The final products were incubated at 37oC and 50oC for 8 weeks and no spoilage was occurred at both temperatures. Furthermore, any significant losses of sensory and physicochemical qualities were not observed.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1999.07a
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• pp.16-21
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• 1999

국민소득의 증대와 더불어 향상된 우리의 식생활 문화는 양보다 품질을 선호하게 되어 청정 농산물의 수요가 증대하였는데 이로 인하여 농작물의 유해성을 감소시킬 수 있으며 기후의 영향을 덜 받아 연중재배 및 생산이 가능한 수경재배 및 양액재배 기술이 등장하였으며, 이를 위한 첨단 시설하우스의 설립도 매우 많이 증가하였다. 또한 점점 더 확산되는 집단육묘 체계에서 살균 처리하여 사용되고 있는 관행 상토를 대체할 수 있는 무균의 배양토 재료의 개발이 관심 대상으로 떠올랐다. 토양 및 광물질, 유기물류가 혼합된 대부분의 상토재료(피트모스, 나무껍질)와 양액재배 지주물질은 매우 많은 양이 수입에 의존하고 있는 실정이다. 이러한 고가의 수입자재를 대체하기 위한 일환으로 우리 농촌에서 농업부산물로 생겨나는 왕겨를 탄화시켜 상토재료 및 양액재배용 지주물질로 사용하려는 연구가 다수 시행되었다. (중략)

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.1-5
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• 1995

Dry-type artificial seeds were produced by dehydrating alginate-encapsulated somatic embryos of lire. When placed on half- strength MS solid medium 20% of the artificial seeds dehydrated to the level of 80% water loss were capable of germination. Addition of 0.1 mg/L ABA to alginate solution before encapsulation enhanced the germination percentage of those dehydrated to the level of nil to 90% water loss by up to 1.7-folds. The results suggest that ABA may enable somatic embryos to overcome physical and/or physiological restraint by encapsulation and dehydration.

• Journal of Embryo Transfer
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• v.9 no.3
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• pp.229-234
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• 1994

This experiment was carried out to develop the model system for mass production of biomedical and nutritional proteins (human proteins) through mamraary gland of the transgenic cattle produced by gene manipulation and embryological technologies. Human growth hormone gene fused with rat $\beta$-casein gene promoter was microinjected into pronuclei of one cell bovine embryos produced by in vitro fertilization. After microinjection, embryos were cultured in vitro for 6 or 7 days. Twenty embryos reaching to blastocysts were transferred to 10 beef recipients, each receiving two embryos. Recipients were diagnosed for pregnancy by rectal palpation at 76 days after embryo transfer. One of them was pregnant to term and produced a female calf weighing 21 kg at 280 days following embryo transfer. DNA was extracted from umbilical cord tissue and blood of calf born for confirming gene insertion. As determined by Southern hybridization, the transgene was not found.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.2 no.1
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• pp.41-46
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• 1969

산란기 직전의 참굴(Crussostrea gigas)을 통상 방법으로 개각하여 0.3 및 2.0 Mrad의 감마선에 각기 쬔 후 $0^{\circ}C$의 온도하에 저장기간중 번식하는 총 세균수와 pH 변화를 측정하고 부패상을 관찰함으로씨 방사선을 쬔 참굴의 저장성을 조사하였다. 자외선을 쬔 무균해수에 개각하지 않고 정화시킨 굴과 보통 해수에 수용하였던 굴을 비교하여 정화 처리에 의한 세균 제거의 효율을 조사하였으나 4-5일의 장기간 처리에도 불구하고 별다른 세균제거 결과를 볼 수 없었다. 이것은 굴이 보유하고 있는 본래의 총 세균수가 적기 때문이라고 추측된다. 감마선을 쬐지 않은 굴은 저장 15일째에 부패했음이 완연했으나 0.3 Mrad의 감마선을 쬔 굴은 35일후 까지도 시큼할뿐 별로 변질되지 않았다. 2.0 Mrad의 감마선을 쪼인 굴은 관능상의 선도 유지에 있어서 0.3 Mrad로 쬔 굴보다 휠씬 나빴고 off-odor가 심했다. 굴 저장중의 pH 변화로 선도를 판정함은 그 신빙성이 극히 희박하였고 선도의 저하와 함께 pH가 내려간다는 일반적인 인식과는 달리 어류부패의 경우와 같이 저장 초기에는 약간 하강하나 부패의 진행과 함께 상승함이 확인되었다.

• Journal of Korean Foot and Ankle Society
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• v.13 no.2
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• pp.236-239
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• 2009

Heel abscesses present as heel pain that progressively worsens, with associated tenderness and fullness a the heel pad. To our knowledge, there are fews reports in the literature describing a spontaneous heel pad abscess. A 48-years old woman presented spontaneously with pain, erythema, edema and increased warmth to this right foot. She has no underlying disease and steroid injection history. A radiologic examination was suggestive of an abscess. Follow incision and drainage, cultures that were taken during the surgical procedure did not produce any organism. The patient was discharged home and recovered from the abscess without recurrence or further surgical intervention.

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.88-92
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• 1988

The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.

• ALGAE
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• v.22 no.3
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• pp.229-234
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• 2007

This study was to determine the extent of bacteria contamination and resistance to various antibiotics used commonly in microalgal culture. Seven different dose levels of chloramphenicol, dihydrostreptomycin sulphate, neomycin, penicillin G, streptomycin sulphate, penicillin G + streptomycin sulphate, and penicillin G + streptomycin sulphate + chloramphenicol were added to each culture of microalgae. The lethal effects on microalgae and bacteria were the highest in chloramphenicol and the lowest in penicillin G. The axenic culture of bacillariophyceae and dinophyceae was more difficult than that of chlorophyceae and haptophyceae because of their complicate external morphology. The efficient antibiotics and their concentrations for axenic cultures varied with microalgal species. The optimum quantity for antibiotic treatments were 2,000 ppm of dihydrostreptomycin for Chlorella ellipsoidea, neomycin 500 ppm of Isochrysis galbana and Heterosigma ahashiwo, hloramphenicol 500 ppm of Cyclotella didymus, and dihydrostreptomycin sulphate and neomycin 6,000 ppm of Thalassiosira allenii.