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The Korean Society of Phycology (한국조류학회(藻類))
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Antibiotics and Their Optimum Concentration for Axenic Culture of Marine Microalgae

해양미세조류의 무균배양을 위한 항생제의 종류 및 최적 농도

  • Youn, Joo-Yeon (Korea Marine Microalgae Culture Center, Department of Aquaculture, Pukyung National University) ;
  • Hur, Sung-Bum (Korea Marine Microalgae Culture Center, Department of Aquaculture, Pukyung National University)
  • 윤주연 (부경대학교 양식학과 한국해양미세조류은행) ;
  • 허성범 (부경대학교 양식학과 한국해양미세조류은행)
  • Published : 2007.09.01
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Abstract

This study was to determine the extent of bacteria contamination and resistance to various antibiotics used commonly in microalgal culture. Seven different dose levels of chloramphenicol, dihydrostreptomycin sulphate, neomycin, penicillin G, streptomycin sulphate, penicillin G + streptomycin sulphate, and penicillin G + streptomycin sulphate + chloramphenicol were added to each culture of microalgae. The lethal effects on microalgae and bacteria were the highest in chloramphenicol and the lowest in penicillin G. The axenic culture of bacillariophyceae and dinophyceae was more difficult than that of chlorophyceae and haptophyceae because of their complicate external morphology. The efficient antibiotics and their concentrations for axenic cultures varied with microalgal species. The optimum quantity for antibiotic treatments were 2,000 ppm of dihydrostreptomycin for Chlorella ellipsoidea, neomycin 500 ppm of Isochrysis galbana and Heterosigma ahashiwo, hloramphenicol 500 ppm of Cyclotella didymus, and dihydrostreptomycin sulphate and neomycin 6,000 ppm of Thalassiosira allenii.

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References

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