• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.117-123
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• 2000

In order to study the role of polyamines on the formation of adventitious roots, trichomes and calli, the effects of putrescine, spermidine, spermine, cyclohexylamine (CHA) and methylglyoxal-bis(guanylhydrazone) (MGBG) were investigated in the leaf segment cultures from ecotype Columbia of Arabidopsis thaliana. When the leaf segments were cultured on the media for forming adventitious roots (0.1 mg/L NAA), trichomes (2.0 mg/L NAA) and calli (10.0 mg/L NAA), and then each cultures was treated with 1-100 mg/L of putrescine, spermidine and spermine, respectively. On the adventitious root-forming medium treated with polyamines the trichomes were induced with adventitious roots. And on the trichome-forming medium with polyamines calli were induced with trichomes. In orther hand each cultures was treated with 1-100 mg/L of CHA and MGBG, respectively. CHA promoted adventitious roots on the medium for adventitious roots, was not effected on media for trichomes and calli. MGBG inhibited adventitious roots, trichomes and calli in all cultures, and induced adventitious roots on medium for trichomes in high concentration.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.211-217
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• 1999

The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thaliana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5mg/L NAA and 0.1mg/L BA for trichome formation, 10mg/L NAA and 10mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1mg/L, trichomes were formed in 2.0mg/L, and calli were formed in 10mg/L. Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.19-27
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• 2001

From two to five-year-old hybrids of Q. serrate ${\times}$ Q. dentate; Q. dentata ${\times}$ Q. serrata, Q. dentate ${\times}$ Q. mongolica var. crispula, and Q. dentata ${\times}$ Q. aliena, intermediate-size of stellate hairs or both types of their parents have been observed. Especially the hybrids between Q. serrate and Q. aliena and Q. aliena and Q. serrate had both types of their parents. The trichomes of the hybrids between Q. fabri and Q. serrate was the characteristics of Q. serrate. It was possible to identify two to five-year-old of interspecific hybrids based on trichome characteristics.

• Applied Microscopy
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• v.38 no.2
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• pp.117-124
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• 2008

Salvinia is an aquatic plant forming dimorphic leaves that have been modified into floating and submerged leaves. A air of floating leaves plays an important role for the floating and photosynthesis while the submerged leaves, which are lim and long, have the form and function of root. Many aquatic plants develop trichomes in the epidermis but in Salvinia, richomes grow densely in the epidermis of the dimorphic leaves. The present study examined the differentiation pattern of trichomes developing in the floating leaves of S. natans and S. molesta by scanning and transmission electron microscopy. Trichomes developing in the floating leaves of Salvinia showed very different patterns. In S. natans, they were arranged in a V-shape form, having 20${\sim}$25 rows at $18{\sim}25^{\circ}$ on both sides of the lamina divided by the midrib in the floating leaf. In each row, 8${\sim}$10 oval-shaped cells, $200{\sim}290{\mu}m$ in length, were arranged in a spiral fashion. Four trichomes of this form made a trichome unit, but their apical parts were separated from one another and developed into the so-called 'knuckle-crane' type. On the other hand, in S. molesta, trichomes differentiated in a unique pattern quite different from those of S. natans. At the early stage of differentiation, trichomes protruded from the epidermis and then 4${\sim}$6 cylindrical cells grew $400{\sim}600{\mu}m$ long and the four trichomes formed as an unit. The four grouped trichomes were interconnected through their apex and developed in the 'egg-beater' type. Then $300{\sim}600{\mu}m$ long multi-cellular stalk cells grew and protruded out of the epidermal surface from the basal part of the trichomes. Such a structural characteristic of trichomes is considered to play a very important role along with the aerenchyma tissue in the leaf mesophyll tissue for the floating of Salvinia on the water surface.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.259-264
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• 1999

In order to study the role of ethylene on the formation of adventitious roots, trichomes and calli, the effects of 1-aminocyclopropane-l-carboxylic acid (ACC), ethephon, $CoCl_2$ and $AgNO_3$ were investigated in the leaf segments from ecotype Columbia of Arabidopsis thaliana. When the leaf segments were cultured on the media for forming adventitious roots (0.1 mg/L NAA), trichomes (2.0 mg/L NAA) and calli (10.0 mg/L NAA), and then each cultures was treated with 1-100 mg/L of ACC and ethephon, respectively. On the adventitious root-forming medium adventitious root formation was decreased, and trichomes were induced. And on the trichome-forming medium trichome formation was decreased, and calli were induced. In order hand each culture was treated with 1-100 mg/L of $CoCl_2$ and $AgNO_3$, respectively. On the adventitious root-forming medium adventitious roots was increased without trichome formation, and on the trichome-forming medium trichome formation was decreased, and adventitious roots were induced. However on the callus-forming medium treated with ACC, ethephon, $CoCl_2$ and $AgNO_3$, respectively, callus formation was inhibited and trichomes were induced in all cultures.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.11-17
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• 1999

Morphological variations in leaves and leaf trichomes according to tree age were investigated for the identification of Quercus taxa at the different stages of development. The sizes of leaves from one-year-old seedlings of Q. serrata, Q. dentata, and Q. aliena were the smallest and increased with tree age. Blade length, blade width, petiole length, number of serration, and serration depth war also varied with tree age. Therefore, the examined taxa by the size and form of leaves were hardly identified at juvenile stages. The type, structure, and density of leaf trichomes of each species varied remarkably along with tree age. Three types of trichomes such as small stellate hairs, large stellate hairs, and long single hairs were observed on the abaxial side of leaves of Quercus taxa. In matured trees of Q. serrata both small stellate and long single hairs on leaves were observed. On the other hand large stellate hairs were found in leaves of Q. dentata. There were small stellate hairs in Q. aliens. In Q. mongolica var. crispula, no hair or small stellate hairs were occasionally found. It is possible to identify Q. serrata and Q. dentata by the characteristics of trichome even at two to three year old seedlings, while, it is difficult to tell Q. aliens and Q. mongolica var. crispula of seedlings from other taxa only by those of trichome.

• Korean Journal of Plant Resources
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• v.13 no.3
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• pp.179-187
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• 2000

The achene morphology about 16 taxa of Korean Aster L. sensu lato were investigated to estimate its taxonomic values. The achene shapes were divided into four types; oblanceolate-oblong, obovate, oblong and obovate-oblong. The trichome shape on achene six types; uniseriate-conical, filiform, cylindrical, capitate type, long stalk capitate and globular. Their distributional features on upper part of achene four types; absent, sparse distribution of conical trichome, dense distribution of conical trichome and mixed distribution of conical and capitate trichome. The achene shapes and trichome characteristics were regarded to be a good characters in delimiting taxa because these did not differ among individuals in same taxa, but differ among the taxa. If Korean Aster L. sensu late were divided into Kalimeris, Heteropappus, Aster, Cymnaster, the capitate forms and mixed distribution of conical and capitate trichome were recognized as the good characters in delimting above section such as genus Kalimeris and Heteropappus, section Pseudocalimeris of Aster L. sensu stricto.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.71-73
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• 1988

The inheritance of stem trichome in red pepper(Capsicum annuum L.) was studied. The parents, $F_1$, $F_2$, $BC_1F_1$ of two combinations, 342 ${\times}$ Kimjanggochu, Namji ${\times}$ Hungarian Wax, were used. Stem pubescent character is controlled by a single gene and pubescent character is dominant to glabrous character.

• Applied Microscopy
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• v.32 no.4
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• pp.319-327
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• 2002

Salvinia natans, an unique water fern having a small rootless body, developed three different types of trichomes throughout the plant. The most peculiar type exhibiting rows of obvious, whitish, multicellular trichome clusters was noticed in the upper surface of the floating leaves. Eight to ten branches within a cluster extended ca. $370{\sim}420{\mu}m$ from the leaf surface. No stalk cell was found, however, four large epidermal cells were discernable at the base of four central branches in the cluster. Each branch consisted of $8{\sim}10$ obliquely-oriented small cells that gradually decreased in size toward the branch tip. The second type was found in the lower surface of the floating leaves, stems, and sporocarps. Multicellular uniseriate trichomes, ca. $430{\sim}980{\mu}m$ long, were distributed all over these structures. The tip of trichome was acicular, but a semi-spheric protuberance of approximately $24{\sim}32{\mu}m$ in diameter occurred at the base of each trichome. The protuberance appeared to be firmly attached to the side of the basal cell, however, internal connection to the trichome cell itself was uncertain. The third type was similar to the second in that multicellur uniseriate trichomes with acicular tip and a protuberance at the base were present. However, the trichomes were considerably long relative to the second type, and only occurred along the surface of highly dissected, submerged leaves. A majority of the trichomes exceeded more than 2 mm in length that hung downward in the water. Regardless of trichome type, all trichomes contained a huge central vacuole with very thin cytoplasm, resulting from the fusion of several vacuoles during early trichome development. The various densely-distributed trichomes formed in Salvinia natans probably play an important role in plant buoyancy.

• The Korean Journal of Pesticide Science
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• v.4 no.2
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• pp.44-49
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• 2000

Objective of this study was to determine the Si distribution and extent of control of powdery mildew diseases of cucumber(Cucumis sativus L.). The distribution of silicon in the leaf epidermis of cucumber plants grown in hydroponic nutrient solutions supplemented with soluble silicates was examined using scanning electron microscopy and energy dispersive X-ray analysis. The silicate absorbed from nutrient solution was translocated into cucumber leaves, and accumulated mainly in the cells surrounding the base of trichome hairs. Base cells surrounding the trichomes also had high levels of Si, Ca, and K. Si levels in the epidermal cells for low Si treatment were not detectable except in the trichome bases. Hyphal lengths of powdery mildew occurring on cucumber leaves cultivated in medium with high concentration of silicate were remarkably shorter than those of cucumber leaves cultivated with low concentration of silicate. There was a negative correlation between hyphal length of S. fuliginea and silicate concentrations.