• Journal of Veterinary Clinics
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• v.31 no.5
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• pp.435-438
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• 2014

An 11-year-old, 2.67 kg female Maltese dog with 3 weeks history of palpable cervical mass near trachea was submitted to a local animal hospital. Radiography and ultrasonography showed radiopaque mass adjacent trachea and vagus nerve. Surgically excised mass was solitary and approximately $3.5{\times}2{\times}0.8cm$ in size. Histopathologically, there were large neoplastic foci admixed with normal thyroid tissues. These neoplastic foci were composed of small to large packets of the neoplastic cells with plasmacytic morphology, and these packets were divided by fine fibrovascular septa. Immunohistochemically, most neoplastic cells in the thyroid mass showed positive reactions for cytokeratin (AE1/AE3), chromogranin A, neuron specific enolase (NSE) and the negative reaction for vimentin. Based on the gross, histopathologic and immunohistochemical characteristics, this dog was diagnosed as medullary thyroid carcinoma.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.619-619
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• 2002

• Proceedings of the Malacological Society of Korea Conference
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• 2002.05a
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• pp.5-5
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• 2002

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1994.04a
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• pp.144.2-144
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• 1994

• Journal of Digestive Cancer Research
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• v.1 no.1
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• pp.48-51
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• 2013

We report an unusual case of postoperative early gastric cancer with liver metastasis mimicking pancreaticobiliary carcinoma. A 73-year-old man with early gastric cancer was transferred for endoscopic treatment. The patient underwent endoscopic submucosal dissection for the treatment of the early gastric cancer. The pathological diagnosis was adenocarcinoma with extension to the deep submucosa and some lymphatic invasion. Therefore, subsequent a subtotal gastrectomy was performed. The histological results demonstrated residual adenocarcinoma confined to the mucosa. The resection margin and lymph node metastasis were negative. Thus, he was closely monitored for recurrence every 6 months. After 2 years, he was suddenly suspected of developing liver metastasis and local recurrence. He received a liver biopsy, and the pathological result was poorly differentiated adenocarcinoma. Immunohistochemical staining suggested pancreaticobiliary carcinoma rather than metastatic adenocarcinoma from the stomach or colon, but primary focus was not found. We were sure that the recurrent stomach cancer metastasized to the liver because stomach cancer can show heterogeneous cytokeratin (CK) expression pattern with various histological features. Therefore, no single CK expression pattern has diagnostic value for distinguishing gastric carcinoma. The patient underwent chemotherapy for metastatic stomach cancer.

• Journal of Veterinary Clinics
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• v.30 no.4
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• pp.296-300
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• 2013

A 7-year-old castrated male Yorkshire Terrier was presented with a palpable intra-abdominal mass. In radiography, a large radioopaque renal mass and small abdominal mass were found on dorsal area of the abdomen. Grossly, red to brown color mass and a cystic structure (hydronephrosis) were embedded in the right kidney. Histopathologically, the mass had many irregular shaped neovascular channels lined by polygonal or oval shaped endothelial cells. These vessels and neoplastic cells had great invasive tendency to adjacent connective or fat tissues. Small abdominal mass had identical morphologic features as in renal mass. According to immunohistochemistry, the neoplastic cells in renal mass demonstrated strong positive signals for vimentin and CD31, and weak positive for von Willbrand factor. However, there were no positive reactions for cytokeratin. Based on the gross, histopathology and immunohistochemistry, this mass was diagnosed as primary renal hemangiosarcoma in a Yorkshire Terrier dog.

• 한국전자현미경학회:학술대회논문집
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• 1999.11a
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• pp.13-17
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• 1999

• The Journal of the Korean bone and joint tumor society
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• v.14 no.2
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• pp.106-118
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• 2008

Purpose: Disturbed cell cycle regulatory proteins are key events underlying the development and/or progression of human malignancies. The aim of this study is to evaluate the protein expression status involved in G1/S cell cycle in human soft tissue sarcoma. Materials and Methods: We simultaneously evaluated the expression of Cyclin D1, Cyclin E, CDK4, CDK2, p16, p27, Rb, E2F1, p53 and Ki-67 by immunohistochemistry in 43 cases of soft tissue sarcoma Results: The Cyclin D1, Cyclin E, CDK4, CDK2, E2F1, and p53 were expressed in 25 (58.1%), 18 (41.9%), 13 (30.2%), 33 (76.7%), 20 (46.5%), and 18 cases (41.9%). The p16, p27, and Rb expressions were decreased in 26 (60.5%), 22 (51.2%) and 19 cases (44.2%). All of the cases showed alterations of more than one out of the above proteins. The increased Cyclin E expression and Ki-67 labeling index (LI) were significantly associated with histologic grade. The Cyclin E and E2F-1 expressions were increased in relapsed cases and the CDK4 expression was increased in cases of metastasis. Conclusion: Alterations of G1/S cell cycle regulatory proteins may play an important role in the tumoriogensis of soft tissue sarcomas. Our results suggest that increased expressions of Cyclin E, E2F1, and CDK4 were associated with tumor relapse or metastasis and could be considered as parameters of prognosis of soft tissue sarcoma.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.937-942
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• 2001

The present study was conducted to identify the mechanism about the regulation of appetite by examining the expression patterns of vasoactive intestinal peptide in the hypothalamus of either fasted for 24 hours or anorexia mutant mouse. In order to investigate expression pattern of the vasoactive intestinal peptide, immunohisto-chemistry was employed along with reverse transcription polymerase chain reaction (RT-PCR) and dot blotting. Immunohistochemistry has shown that level of expression of vasoactive intestinal peptide and appetite-suppessing neuropeptide, was lower in the suprachiasmatic nucleus (SCN) and higher in the paraventricular nucleus (PVN) of the anorexia mutant group than in the comparable regions in the control group. This pattern was repeated in the fasting group, which also showed lower and higher levels of vasoactive intestinal peptide expression in the SCN and PVN respectively, In contrast, the vasoactive intestinal peptide mRNA level in the entire hypothalamus via RT-PCR and dot blotting was similar in the fasting and control groups, while it was significantly increased in the anorexia mutant group.

• The Korean Journal of Zoology
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• v.37 no.3
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• pp.385-408
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• 1994

출생후 0일. 7일. 14일 및 21일 그리고 성체의 흰쥐 전뇌 기저부의 내측중격핵, 수직 및 수평 대각 Broca대 거대세포 시삭전핵 그리고 복부담창구에서 신경성장인자수용체 (nerv-growth 배ctor receptor, NGFr)에 면역반응을 보이는 신경조직과 세포의 분화를 면역조직화학적 및 전자현미경적 방법을 이용하여 조사하였다. 출생후 초기와 성체에서 신경세포 원형질막 뿐만 아니라 세포질에서 NGFr 면역반응이 확인되었다. 그러나 성체에서 신경세포 원형질 막에서의 면역반응은 관찰되지 않았다. 특히 NGFr 면역반응은 골지 부위에서 보였고, 점상의 면역반응물들이 세포체의 세포질과 수상돌기에 소수 분산 분포하였다. 뇌 기저부의 NGFr 면역반응 신경세포들은 뇌 크기의 증대와 뇌 조직의 분화에 따라 점차 수적 증가를 보였다. 이 NGFr 면역반응 신경세포들은 세포의 모양과 세포체의 장 .단축의 비에 따라 6가지 형. 즉 1) 원형. 2) 타원형. 3) 세장형, 4) 방추형, 5) 삼각형, 6) 다각형으로 분류되었다. 전뇌 기저 핵에서 원형과 난형신경세포들의 출현율은 출생후 0일에서 높았으나 성체로 되면서 감소된 반면, 세장형. 방추형, 삼각형 그리고 다각형신경세포들의 출현율은 출생후 0일에서는 낮았으나 성체로 되면서 증가하였다. 모든 핵들에서 NGFr 면역반응 신경세포체의 부피는 출생후 0일에 759-1,640 Um3로 제일 작았으며, 수직 대각 Broca대와 복부담창구에서는 출생후 14일에 각각 5 107 7.385 Um3 그리고 내측중격핵, 수평 대각 Broca대, 거대세포 시삭전핵에서는 출생후 21일에 각각 4,705, 6,061, 6,412 Um3로 최대치를 보였다. 그후 성체로 되면서 모든 핵에서 1,893-3,464 $\mu$m3로 다시 감소하였다. 전자현미경적 관찰에서 출생후 21일된 흰쥐 수평 대각 Broca대에서 NGFr 면역반응은 세포체와 수상돌기의 원형질막 그리고 세포체내에서는 골지체, 다소포성소체 및 조면소포체에서 관찰되었다. 이 결과들로 미루어 NGFr은 출생후 발생단계와 성체의 횐쥐 전뇌 기저부에서 신경세포의 분화와 분포에 관계되는 것으로 생각된다.