• Journal of Dairy Science and Biotechnology
• /
• v.15 no.1
• /
• pp.1-9
• /
• 1997

This study was conducted to efficiently separate the Ig from Korean native cattle colostrum and to utilize them as an immunogen for the production of antibodies aginst rabbit. The results obtained were as follows : 1. About 84% of Ig G could be separated from Korean native cattle colostrum by·gel filtration using Superose 12 column on HPLC. The separation profile of Korean native cattle colostral immunoglobulin was similar that of Holstein colostral Ig. 2. Separation of Korean native cattle colostral Ig by anion exchange chromatography using Mono Q column on HPLC was poor resolution chromatographic pattern. 3. Hi-Trap Protein G column showed better results than the Protein A Sepharose CL-4B column in the Ig G binding capacity from Korean native cattle colostral Ig. 4. Protein G Sepharose Fast Flow system resulted in higher Ig g binding capacity as the industrial size scale-up approach. 5. Sufficient titer reaction of antibody to Korean native cattle colostral Ig G was confirmed by ELISA.

• Journal of Industrial Convergence
• /
• v.19 no.6
• /
• pp.187-195
• /
• 2021

This study was conducted to identify the characteristics of immune function and inflammatory changes for elderly women, by having them to participate in line dance program. For this purpose, 18 elderly women in their 60s to 70s, living in Y city were selected, and 9 of the participants were randomly assigned to participate in the line dance program for 12-weeks (line dance exercise group), while the other 9 participants were assigned to carry out their daily lives without participating in certain physical activity (control group) for the same period. The subjects' immune function and inflammation were measured using SPSS 24.0 for datafication, and the average and two-way ANOVA analysis were conducted. The result indicates the immunoglobulin, IgA, has increased, but not dramatically. The level of IgG(p<.05), and IgM(p<.05) increase were detected from the line dance exercise group, but not so much from the control group. Inflammation indicator, the CRP(p<.05), were significantly reduced from the line dance exercise group, but not much for the control group. According to the collected data, line dance for the elderly women can be considered as an effective exercise program, and the result shows that inflammation is reduced and immune function is improved. It also implies the needs of active physical activity arbitration to keep the elderly women's health and to prevent the chronic disease in the future.

• The Journal of the Korean dental association
• /
• v.23 no.7 s.194
• /
• pp.617-624
• /
• 1985

• Clinical and Experimental Pediatrics
• /
• v.49 no.9
• /
• pp.987-990
• /
• 2006

Intravenous immunoglobulin (IVIG) infusion is an effective therapy for acute Kawasaki disease (KD). Nonetheless, approximately 10 percent to 20 percent of patients have persistent or recrudescent fever despite IVIG treatment, leading to a higher risk for coronary artery aneurysms (CAA). This unresponsiveness may pose a challenge to the clinicians. Tumor necrosis $factor-{\alpha}$ levels are elevated in the acute phase of the disease, especially in patients who develop CAA. We report a 10-month-old male with KD who failed to respond to multiple doses of IVIG and methylprednisolone and who then was treated with infliximab (5 mg/kg single dose). After infliximab treatment, he became afebrile with normalization of inflammatory markers and no further progression of CAA.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2001.11a
• /
• pp.130-130
• /
• 2001

• Journal of the Korea Convergence Society
• /
• v.6 no.5
• /
• pp.329-336
• /
• 2015

In this study, Pilates mat exercise program three times a week for 8 weeks, 10 minutes for a warm up, 40 minutes of main exercise, and 10-minute cool down exercise using gadgetry to the middle aged women. Pilates exercise program when exercise intensity based on the instructions of the ACSM (2010) as 1-4 weeks 50-60% HRR, 5-8 weeks 61-70% HRR to give 50-70%, maintaining the strength of 4 weeks. Pilates exercise program by gadgetry has increased the strength of wireless heart rate meter (Polar Analyzer, Polar Electro of Finland) Karvonen (1979), proceed individual target heart exercise obtained as a way now to check your strength workout through individual conciousness. We estimated immunoglobulin change for Pilates mat exercise by gadgetry during 8-week. It showed the following conclusions. First, immunoglobulin IgA has been increased and Interaction effects has been showed thruough Pilates mat exercise programs for 8 weeks. Second, immunoglobulin IgG has been increased and showed Interaction effects has been showed thruough Pilates mat exercise programs for 8 weeks. Third, immunoglobulin IgM has been increased and showed Interaction has been showed effects thruough Pilates mat exercise programs for 8 weeks. Therefore, this study was conducted on a Pilates mat exercise using props immune function are positive hormonal changes, can prevent private for the disease and effective exercises that are the fodder.

• Journal of Periodontal and Implant Science
• /
• v.29 no.2
• /
• pp.349-355
• /
• 1999

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.

• Pediatric Infection and Vaccine
• /
• v.22 no.3
• /
• pp.206-209
• /
• 2015

We report the case of a 7-year-old boy who showed treatment-nonresponsive hypotension (59/29 mmHg) and decreased left ventricular systolic function (fractional shortening 22%) in the acute stage of Kawasaki disease (KD). The present case serves to highlight that methylprednisolone pulse therapy should be considered in patients with intravenous immunoglobulin nonresponsive symptomatic myocarditis during the acute stage of KD.

• Journal of Animal Science and Technology
• /
• v.45 no.4
• /
• pp.537-542
• /
• 2003

Sixty [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs (7.63$\pm$0.41kg average body weight and 25-d average age) were used in a 20-d growth assay to determine the effect of dietary recombinant human lactoferricin culture (RHLC) supplementation on growth performance, digestibility and plasma IgG concentration in weaning pigs. Dietary treatments included 1) Negative control (NC : without antibiotic), 2) Positive control (PC : NC diet + 0.1% chlortetracycline), 3) RHLC0.3 (NC diet + 0.3% RHLC), 4) RHLC0.5 (NC diet + 0.5% RHLC). No differences were found among treatments in average daily gain (P>0.05). ADFI of pigs fed RHLC0.3 diet was higher than that of pigs fed PC diet (P<0.05). However, pigs fed RHLC0.5 diet had improved gain/feed compared to pigs fed PC diet. Pigs fed PC and RHLC diets showed significantly increased dry matter digestibility compared to pigs fed NC diet (P<0.05). There was no significant difference in plasma IgG concentrations (P>0.05). The supplementation of RHLC in starter pig diets appears to be an alternative to antibiotics.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.13 no.3
• /
• pp.175-180
• /
• 2009

Purpose: Detection of TSH-binding inhibitor immunoglobulin (TBII) in patients with hyperthyroidism is an important result of Graves' disease (GD) and hyperthyroidism treatment. This has been made out an inspection by commercial radio-receptor assays. To increase the sensitivity and the specificity of the assay, many results of the assay were reported. In this study we evaluated the clinical usetulness of TBII assays by the Comparative method. Material and Methods: We were measured by using healthy control group (n=30, male=20, female=10) of Seoul National University Hospital Healthcare System Gangnam Center from January to March in 2009. Similarly, We were measured by using hyperthyroid (TSH<$0.05\;{\mu}IU/mL$, FT4>1.80 ng/dL) experimental group (n=58, male=14, female=44) of division of endocrinology and metabolism department of internal medicine Seoul National University Hospital from January to March in 2009. We made a comparative study of each two assays from the first generation to the third generation. We were used of TSAb assay as a measurement of GD diagnostic technique. Results: The specificity of healthy control group was 100% according to the generation. (Specificity=100%, n=30) The sensitivity of hyperthyroid experimental group were the first generation RSR<%> (79.3%, n=58), RSR (51.7%, n=58), the second generation RSR-CT (93.1%, n=58), BRAHMSCT (98.3%, n=58), the third generation ELISA (94.6%, n=56), ECLIA (97.7%, n=58) and TS-Ab<%> (93.5%, n=46). Conclusion: We were used of TSAb assay as a measurement of GD diagnostic technique, The result of data showed a high correlation between the third generation TBII assay and the second generation TBII assay ($R^2$=0.923). Instead of the first generation assay, the second generation assay can be more useful in clincal diagnosis.