• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.263-266
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• 2012

Isolation of iron and calcium-binding peptides derived from cottonseed meal protein (CMP) hydrolysates was investigated. The degree of hydrolysis of CMP by Flavourzyme was monitored using trinitrobenzenesulfonic acid method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Enzymatic hydrolysis of CMP for 12 h was sufficient for the preparation of CMP hydrolysates, and the hydrolysates were membrane-filtered under 3 kDa as a molecular weight. The filtered solution was fractionated using Q-Sepharose fast flow, Sephadex G-15, and reversed phase-high performance liquid chromatography for iron and calcium-binding peptides. As a result, F51 fraction was obtained as the best candidate for calcium and iron chelation, and the isolated iron and calcium-binding peptides can be used as functional food additives, similar to iron and calcium supplements.

• Korean Journal of Poultry Science
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• v.20 no.4
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• pp.189-196
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• 1993

True amino acid availability (TAAA) and true metabolizable energy(TME) values of 8 protein feedstuffs were determined by feeding three roosters exactly 30g of each protein feedstuff after 36h of fasting. From each rooster excreta were collected for 36 h. TAAA were significantly(P<0.01) different among protein feedstuffs. TAAA was highest in fish meal(96.1%), followed by corn gluten(91.2%), rapeseed meal(88.8%), soybean meal(88.7%), meat meal(87.2%), canola meal(86.1%), cottonseeed meal(82.6%) and feather meal(82.5%). Available Iysine values obtained by TAAA method were highly correlated(P<0.01) with those obtained by chick bioassay(CBA) and FDNB method. TME was highest in corn gluten(4,011kcal/kg, as fed basis), followed by fish meal(3,906), feather meal(3,098), soybean meal(3,007), meat and bone meal(2,631), canola meal(2,326), cottonseed meal(2,246) and rapeseed meal(2,120).

• Journal of Aquaculture
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• v.11 no.1
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• pp.19-29
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• 1998

An 18-week growth trial was conducted in flow-through aquarium system to evaluate the practical dietary protein sources for juvenile abalone (Haliotis discus hannai). Three replicate groups of the abalone averaging 0.11g were fed one of ten diets containing casein, white fish meal (WFM), meat meal (MM), feather meal (FM), blood meal (BM), soybean meal (SM), corn gluten meal (CGM), cotton seed meal (CSM), Undaria powder (UP), or wheat flour (WF) as a dietary protein source. In addition, these dietary protein sources were cmpared with algae such as raw Undaria or dried Laminaria. Weight gain of abalone fed the diets containing casein, WFM, SM, CSM, or UP was significantly higher (P<0.05) than those of abalone fed other diets, and this value of abalone fed FM, BM, CGM, or algae was lower than other groups. Shell length, shell width, body wt./shell length ratio, and body wt./shell width ratio of abalone fed casein, WFM, SM, CSM, UP, and WF were also highe (P<0.05) than those of other groups. There were no significant difference (P>0.05) in moisture and protein contents of soft body among all diets. The data obtained in this study indicate that each of the casein, WFM, SM, CSM or UP is good dietary protein source for juvenile abalone.

• Journal of Mushroom
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• v.18 no.2
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• pp.141-144
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• 2020

In this study, we attempted to find alternative materials to replace Cotten seed meal(CM) using the wastes of bean sprouts by-product in bottle cultivation of Pleurotus ostreatus. In Proximate analysis, the protein content of BW was slightly lower than that of CM, but the growth characteristics of mushrooms were similar to those of control when byproduct of bean sprouts was used. In addition, the amino acid composition of fruit body did not show a significant difference in the treatment section. Therefore, it is judged that BW could be a suitable substitute for CM in the cultivation of Pleurotus ostreatus.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.351-358
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• 2007

This experiment was conducted to determine correlation between in vitro protein fractions and in situ protein degradation rate with major dairy protein sources(soybean meal, corn gluten meal, cotton seed meal, kapok seed meal and perilla meal). Five protein fractions were obtained according to the Cornell Net Carbohydate and Protein System(CNCPS), and in situ protein degradation rates were determined by technique using nylon bags incubated for 0, 4, 8, 12 and 24hrs in the rumen of three Holstein steers. Fraction A was highest in kapok seed meal(14.6%) and lowest in corn gluten meal(0.6%) (P<0.05). The highest B1, B2 and B3 fractions were contained in soybean meal(8.27%), cotton seed meal(74%), and perilla meal(40%), respectively. Corn gluten meal was very high in fraction C. In situ protein degradation rate of soybean meal was 98%, highest among five protein sources, and corn gluten meal had the lowest rate at 28%. Correlation analysis showed that easily soluble fractions of both methods, in situ protein degradation rate and digestible protein fractions, and in situ protein degradation rate minus “a” and fraction B2+B3 were highly correlated. These results indicate that in vitro protein fractionation can be used in the estimation of in situ protein degradation.

• Korean Journal of Food Science and Technology
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• v.6 no.3
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• pp.138-146
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• 1974

The present study was undertaken to investigate the nutritive value of various defatted oil-seed flours in the diet by studying the effect of alternative diets on growing rats, to attention growth, food intake, weight of organs and the levels of total cholesterol, phospholipid and triglyceride in serum and liver tissue. Sixty male Albino rats (Splague Dowley) weighing 47-55 g were divided into five experimental groups and one control group of ten each, and were fed for 24 weeks with the respective diets. Each of five different diets was supplemented with defatted flours as dietary protein sources. Four of other diets were composed of 7.5 sesame seed, rapeseed, sunflower seed and cottonseed flour respectively, with the same amount of defatted soybean flour, while the fifth diet tested was prepared with 15% soybean flour. The results of this study were as follows. 1) It was noteworthy that the five defatted oilseed flour diets demonstrated much improvement of nutritive value. Sesame seed flour and sunflower seed flour, especially appeared to be worthwhile as human foods. 2) From the results of biochemical tests of the serum and liver lipid content of Albino rats, after feeding with the respective diets, it can be concluded that, from a nutritional point of view, the toxic effects of oilseed flours like rapeseed and cottonseed flour, which contain toxic substances, are reduced when they are mixed with soybean flour.

• Journal of Aquaculture
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• v.11 no.2
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• pp.133-140
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• 1998

A 20-week growth trial was conducted in flow-through aquarum system to investigate the practical dietary carbohydrate sources for juvenile abalone (Haliotis discus hannai). Four replicate grops of the abalone averaging 0.125g were fed one of eight diets containing 24.2% wheat flour (WF), 20% dextrin (DEX), 20% sucorse (SUC), 10% $^{\alpha}$-potato starch+10% $^{\beta}$-potato starch (ab-S), 15% $^{\alpha}$-potato starch (a-S15), 20% $^{\alpha}$-potato starch (a-S20), 25% $^{\alpha}$-potato starch (a-S25), or mixture (MIX) with practical ingredients such as soybean meal, corn gluten meal, cotton seed meal and heat flour. In addition, these formulated diets were compare with macroalgae such as dried sea mustard Undaria (D-SM) or dried sea tangle Laminaria(D-ST). Survival rate, weight gain, shell growth and soft body weight of abalone were not significantly affected by the different dietary carbohydrate sources (P>0.05), whereas those fed a-S15 diet were slightly low. These values of abalone fed D-ST were lowest (P<0.05), followed by those fed D-SM. Lipid contents of soft body from abalones fed a-S25, D-ST or D-SM were significantly lower than those of abalone fed other diets (P<0.05). These data indicate that abalone can equally utilize any carbohydrate sources used in this study.

• Journal of Animal Environmental Science
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• v.16 no.2
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• pp.143-152
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• 2010

Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

• Korean Journal of Agricultural Science
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• v.23 no.2
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• pp.188-205
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• 1996

This study was conducted to examine the effects of dietary energy and protein sources on the rumen microbial population of sheep. The results obtained were summarized as the follows; 1. Ruminal pH was highest in sheep fed the diet rapidly degraded in the rumen(F-F) as a energy (barley plus beet pulp) and protein source (rapeseed meal), and lowest in the diet (F-S) of rapidly degradable energy source plus slowly degradable protein source (corn gluten meal + cotton seed meal) without affecting by postfeeding time. 2. Ruminal ammonia concentration was higher in corn (slowly degradable) for energy source and rapeseed meal (rapidly degradable) for protein source (S-F) than others, and abruptly increased at 1 hr after feeding regardless of treatments. 3. Concentration of ruminal total volatile fatty acid, acetate and propionate were highest in F-S and peaked at 1 hr after feeding firstly and formed second peak at 9hrs, respectively. 4. Digestibilities of the proximates and ADF were not affected by treatment but NDF was highest in F- F. 5. Nitrogen retention was highest in S-F and lowest in F-F and F-S. 6. Digestibility and metabolizability of energy, contents of OCP, TDN, DE and ME were not affected by treatment.