• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.33-48
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• 2012

As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.18-25
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• 2015

A universal DNA barcoding for agricultural noxious weeds is a powerful technique for species identification without morphological knowledge, by using short sections of DNA from a specific region of the genome. Two standard barcode markers, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used to examine the effectiveness of the markers for Pooideae barcoding using 163 individuals of 29 taxa across 16 genera of Korean Pooideae. The rbcL and ITS revealed a good level of amplification and sequencing success while matK did not. Barcode gaps were 78.6% for rbcL, 96.2% for matK, and 91.7% for ITS, respectively. Resolving powers were 89.3% for rbcL, 92.3% for matK, and 79.1% for ITS. The matK obtained the best both barcode gap and resolving power. However, it should be considered not to employ matK for Pooideae barcode because of low rate of PCR amplification and sequencing success. As a single DNA marker, rbcL and ITS were reasonable for Pooideae barcode. Barcode gap and resolving power were increased when ITS was incorporated into the rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use.

• Journal of Life Science
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• v.27 no.4
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• pp.464-470
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• 2017

Three Korean native cattle (KNC) and seven exotic breeds (Chikso, Hanwoo, Jeju black, Holstein, Japanese black, Charolais, Angus, Hereford, Simmental, and Cross breed) were characterized by using five microsatellite (MS) markers (INRA30, TGLA325, UMN0803, UMN0905, and UMN0929) from the sex chromosome. Genetic diversity was evaluated across the 10 breeds by using the number of alleles per locus, allele frequency, heterozygosity, and polymorphism information content (PIC) to search for locus and/or breed specific alleles, allowing a rapid and cost-effective identification of cattle samples, avoiding mislabeling of commercial beef. It was divided into two main groups from STRUCTURE analysis, one corresponding to KNC and the other to exotic cattle breeds. These results also showed specific genetic differences between KNC and exotic breeds. Nei's standard genetic distance was calculated and used in the construction of a neighbor-joining tree. Results evidenced a correspondence between genetic distance, breeds' history, and their geographic origin, and a clear separation between KNC and exotic breeds. Overall, this study evidenced that DNA markers can discriminate between domestic and imported beef, contributing to the knowledge on cattle breeds' genetic diversity and relationships by using MS markers of the sex chromosome. These markers would be useful for inhibitory effect about false sales and for building an effective tracking system.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.143-148
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• 2007

The introduced exotic species has often caused severe problems to the native ecosystem. One of such species is the freshwater fish gengorobuna (Carassius cuvieri) introduced from Japan. The first step to assess harmful effects of this species on the Korean freshwater ecosystem is to discriminate it from the most similar native crucian carp (Carassius auratus). Because traditional morphological identification often gives unreliable results due to their highly similar phenotype, a new more efficient method is needed. For this purpose, molecular markers produced by the efficient one-step PCR method using three primers (DDF, DDR and DDR1) were developed and tested in the present study. This molecular marker will play an important role in monitoring fish community of Korean freshwater ecosystem.

• 한국HCI학회:학술대회논문집
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• 2008.02b
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• pp.41-46
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• 2008

디지털 기술의 발전은 인간 생활에 큰 변화를 가져다주었다. 이와 같은 디지털 기술의 발전으로 변화된 인간 생활 중 가장 큰 변화는 기술의 발전 이전에 사용되던 각종 제품 및 도구들이 디지털 기술의 힘으로 새롭게 재탄생하고 있다는 점이다. 재탄생된 제품 및 도구들은 사람과 제품간의 경계를 무너뜨리고 서로 융합되고 있다. 이러한 제품들 중에서 대표적으로 Digital Mirror를 들 수 있다. 기존 인간 생활에서 사용되던 일반적인 Mirror가 최근 들어 Digital Mirror라는 새로운 형태의 제품으로 연구되어 지고 있다. 이러한 Digital Mirror는 일상생활 속에서 기존 거울을 대체하고 있으며, 인터렉티브 미디어 아트에서도 많이 응용되고 있다. 현재 Digital Mirror는 광고, 인터렉티브 아트 등의 분야에서 다양한 형태의 콘텐츠가 개발되고 있다. 하지만 Digital Mirror를 새로운 제품 산업군으로 확대하여 신사업을 창출하기 위해서는 수많음 사용자들의 욕구를 충족시킬 수 있는 인터렉티브한 콘텐츠 제작을 해야 한다. 본 연구에서는 Digital Mirror의 인터렉티브한 콘텐츠 제작을 목적으로, 기존의 인터렉티브한 콘텐츠 사례를 중점적으로 연구하여 새로운 콘텐츠 아이디어를 도출하였다. 아이디어를 통해 어린이 사용자를 타켓으로 새로운 사용경험을 제공해 줄 수 있는 컨셉을 정하여 인기 애니메이션 캐릭터를 사실적으로 체험할 수 있게 해주는 콘텐츠 제작방향을 설정하여 연구를 진행하였다. 또한 놀이와 참여, 상호작용을 통한 새로운 디지털 놀이 도구라는 개념으로 AR기술을 이용해 캐릭터를 좀더 사실적으로 체험 할 수 있도록 제작하였다. 어린이 사용자가 캐릭터 마커를 착용하고 Digital Mirror앞에 서게 되면 캐릭터 마커에 해당되는 3차원 입체영상 애니메이션 캐릭터가 어린이 사용자의 모습과 합성되어 사실적인 캐릭터 콘텐츠를 체험 할 수 있게 해 주는 콘텐츠이다. 어린이들에게 인기 있는 캐릭터를 3D로 제작하여 캐릭터 소스로 활용하였고 이를 효율적으로 사용할 수 있도록 어플리케이션 저작툴을 개발 완료하여 제작하였다. 그리고 평판 디스플레이를 이용하여 실제 Prototype 제작하였다.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.745-752
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• 2014

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is an economically important vegetable crop as a source of the traditional food Kimchi in Korea. Although many varieties exhibiting desirable traits have been developed by the conventional selective breeding approach, breeding related to abiotic or biotic stresses, such as a particular pests or diseases, or tolerance to climatic conditions, is likely to be slow. This could be helped by an efficient method for selection from various, rapidly-evolved genetic resources on the basis of molecular markers. In particular, the Brassica genome sequencing project enables genome-wide discovery of genes or genetic variants associated with agricultural traits. We here discuss the recent progress in the field of Chinese cabbage breeding with regard to the application of molecular markers.

• Journal of Life Science
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• v.14 no.2
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• pp.339-344
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• 2004

Identification of individual quantitative trait loci (QTL) is a prerequisite to application of marker-assisted selection for stern length. Two simple sequence repeat (SSR)-based linkage maps were constructed from recombination inbred line populations between cross of Keunolkong and Shinpaldalkong. Two parents used differed greatly in stem length, which were 30.57 cm and 49.75 cm in Keunolkong and Shinpaldalkong, respectively. Using the constructed maps, regression analysis and interval mapping were performed to identify QTLs conferring stem length. Four QTLs for stem length on linkage groups (LG) F, J, N and O were identified in the Keunolkong ${\times}$ Shinpaldalkong population and they totally explained 37.83% of variation for stem length. In the population, two major QTLs on LG J and O conditioning 14.25% and 10.68% of the phenotypic variation in stem length were determined and two QTLs with minor effect were detected on LG F and N. Identification of QTLs for stem length and mapping individual locus should facilitate to describe genetic mechanisms for stem length in different population. SSR markers tightly linked to QTLs for stem length allow to accelerate the elimination of deleterious genes and selection for desirable recombinants at early stage in crop breeding programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.185-192
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• 2006

This experiment was conducted to assess genetic diversity of waxy corn inbred lines and to identify SSR markers related to major characteristics affected kernel quality for improving waxy corn $F_1$ hybrid with good quality. Diversity of 64 waxy com inbred lines was evaluated using 30 microsatellite markers. The 30 microsatellite markers representing 30 loci in the maize genome detected polymorphisms among the 64 inbred lines and revealed 225 alleles with a mean of 7.5 alleles per primer. The polymorphism Information content (PIC) value ranged from 0.14 to 0.87, with an average of 0.69. Based on Nei's genetic distances, the 64 inbred lines were classified into 9 groups by the cluster analysis. The group I included 26 inbred lines (41%), other groups included 3 to 9 inbred lines. One-way analysis of variance was conducted to identify significant relationship between individual markers and major characteristics that affect kernel quality. The analysis showed that umc1019 was related to amylopectin and crude protein content, me 1020 to amylopectin content and peak viscosity, and bnlg1537 to 100-kernel weight, kernel length, and kernel width.

• Korean Journal of Plant Resources
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• v.32 no.5
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• pp.519-542
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• 2019

Twenty soybean cultivars developed recently were assessed using 27 insertion and deletion (InDel) markers derived from dense variation blocks (dVBs) of soybean genome. The objective of this study is to identify the distinctness and genetic relationships among a total of 169 soybean accessions including new cultivars. The genetic homology between 149 accessions in the soybean barcode system and 20 new cultivars was 61.3% on average with the range from 25.9% to 96.3%, demonstrating the versatile application of these markers for cultivars identification. The phylogenic analysis revealed four subgroups related to their usage. The 80% of cultivars for vegetable and early maturity and the 65.9% of cultivars for bean sprouts were clustered in subgroup I-2 and II-2, respectively, indicating of the limited gene pools of their crossing parents in breeding. On the other hands, the cultivars for soy sauce and tofu with considerable gene flow by genome reshuffling were distributed evenly to several subgroups, I-1 (44.4%), I-2 (26.4%) and II-2 (23.6%). We believe that the 27 InDel markers specific to dVBs can be used not only for cultivar identification and genetic diversity, but also in breeding purposes such as introduction of genetic resources and selection of breeding lines with target traits.

• Proceedings of the Korean Society of Computer Information Conference
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• 2022.07a
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• pp.563-566
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• 2022

본 논문에서는 증강현실에서 객체 증강 시, 특징점과 GPS를 이용하여 증강 위치를 효율적으로 보간할 수 있는 ICP(Iterative closest point) 매칭 기법을 제안한다. 다양한 환경에서 제한받지 않고 객체를 증강하기 위해 일반적으로 마커리스(Markerless) 방식을 사용하며, 대표적으로 평면 검출과 페이스 검출을 사용한다. 이는 현실과 자연스러운 동기화를 위한 것으로 계산은 작지만, 인식의 범위가 넓기 때문에 증강 위치에 대한 오차가 존재한다. 이러한 작은 오차는 특정 산업에서는 치명적일 수 있으며, 특히 건설이나 의료시설에서 발생하면 큰 사고로 이어진다. 객체를 증강 시킬 때 해당 환경에 대한 점 구름(Point cloud)을 수집하여 데이터베이스에 저장한다. 본 논문에서는 관측되는 점 구름과의 오차를 줄이기 위해 ICP 매칭 기법을 사용하며, 실린더 기반의 각도 보간을 이용하여 계산량을 줄인다. 결과적으로 특징점과 GPS를 이용하여 ICP 매칭 기법을 통해 효율적으로 처리함으로써, 증강 위치에 대한 정확도가 개선된 증강 방식을 보여준다.