• Title/Summary/Keyword: 래디칼

Search Result 15, Processing Time 0.027 seconds

Effect of Free Radical Scavenging from Green Tea Extraction using Carbon Dioxide Fluid (이산화탄소 유체를 이용한 녹차의 용매 추출물의 래디칼 소거 기능)

  • Kang Ok-Ju
    • Korean journal of food and cookery science
    • /
    • v.21 no.6 s.90
    • /
    • pp.936-941
    • /
    • 2005
  • Green tea was extracted with different solvents such as hot water, varying concentrations of ethanol and carbon dioxide fluid. The carbon dioxide fluid extraction of green tea was a very effective extraction method in terms of antioxidant yield and stability. At pressure of 125kgf, antioxidant extract of green tea produced a yield 1.87 and 2.8 times higher than hot water and $95\%$ ethanol extract, respectively. Antioxidant extract of carbon dioxide fluid was also very stable during 7-day storage at 35?.

Antioxidative Activity and Quality Characteristics of Kochujang Amended with Different Ratios of Deodeok (Condonopsis lanceolata) Root Powder (더덕분말을 첨가한 고추장의 품질 특성 및 항산화 활성)

  • Kim, Ok-Sun;Sung, Jung-Min;Ryu, Hye-Sook
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.22 no.5
    • /
    • pp.667-676
    • /
    • 2012
  • This study was conducted to investigate the quality characteristics and antioxidant activity of Kochujang following the addition of deodeok (Condonopsis lanceolata) powder. To accomplish this, 1%, 3% and 5% deodeok powder was added to Kochujang and the samples were then incubated at $30^{\circ}C$. during which time the general ingredients, water content, acidity, salinity, reducing sugar content, amino-nitrogen content and DPPH were analyzed at intervals of about two weeks. The water crude protein, crude fat and ash content were 3.61%, 8.44%, 4.62% and 8.85%, respectively. The initial acidity was 3.23~3.97%, and this increased to 4.39~44.86%. highlight please clarify this, I cannot infer your intended meaning; however, this can likely be deleted. The salinity was 7.42~7.73%, and 5% with deodeok powder producing the lowest content and showing a tendency to decrease during the storage period. The early amino-nitrogen content was 274.0~333.1%, with higher nitrogen contents being associated with large amounts of deodeok powder. The amino-nitrogen content during the storage period increased to the 4th week, then decreased. Additionally, the a value decreased sharply during the 4-week storage period. Addition of large amount of deodeok powder significantly improved the free radical scavenging activity. The free radical scavenging activity of 1%, 3% and 5% deodeok powder was higher than that of Kochujang throughout the maturation period. Overall, the results of this study indicate that there is the potential to develop functional foods by the addition of deodeok.

Radical Sulfonation of Condensed Tannins (축합(縮合)탄닌의 래디칼 설폰화(化))

  • Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
    • /
    • v.18 no.3
    • /
    • pp.69-76
    • /
    • 1990
  • $^1H$$^{13}C$ 핵자기(核磁氣) 공오(共嗚) 분광기(分光器), 적외선(赤外線) 흡광(吸光) 분광기(分光器)와 질량분석기(質量分析器)를 이용(利用)하여 설폰화(化) 반응(反應)의 한 반응물(反應物)이 단리(單離)되어 구조적(構造的)으로 규명(糾明)되었다. 그 화합물(化合物)은 에탄올 및 에탄올 수용액(水溶液)을 용매(容媒)로 사용(使用)하여 Sephadex LH-20상(上)에서의 반복적(反復的)인 컬럼 크로마토그래피로 단리 및 정제(精製)되었다. 규명된 화합물은 이전에 보고(報告)된 바 없는 새로운 구조의 disodium epicatechin-(4${\beta}$, 5')-disulfonate였다. 이 화합물은 온화(溫和)한 설폰화(化) 조건하(條件下)에서 최초(最初)의 친전자(親電子) 치환(置換)의 예(例)로서 catechol B 환구조(環構造)가 quinone methide 중간물(中間物)을 생성(生成)하는 한 반응기구(反應機構)가 제시(提示)되었다.

  • PDF

Changes of Characteristics in Kochujang Fermented with Maesil (Prunus mume) Powder or Concentrate (매실분말 및 농축액을 첨가한 고추장의 숙성중 품질 변화)

  • Park, Woo-Po;Cho, Sung-Hwan;Lee, Seung-Chul;Kim, Sung-Yong
    • Food Science and Preservation
    • /
    • v.14 no.4
    • /
    • pp.378-384
    • /
    • 2007
  • Powder, or a concentrate, of maesil (Prunus mume) were used in the making of kochujang, and were added to kochujang at 0.5 or 1.0% (w/w). Kochujang fermented with maesil powder lower moisture content than did samples fermented with maesil concentrate. Moisture content slowly increased during fermentation, and was $3{\sim}4%$ higher than the initial value after 8 weeks of fermentation. Kochujang fermented with maesil powder or maesil concentrate showed lower pH values and higher total acidities than did the control. The total microbial count was $3.6{\times}10^7\;5.4{\times}10^7\;cfu/g$, and yeast and mold levels were $2.0{\times}10^7{\sim}3.6{\times}10^7\;cfu/g$, as kochujang fermentation commenced. Microbial load reached a maximum by 4 weeks of fermentation, and decreased thereafter. L (lightness), a (redness) and b (yellowness) values of kochujang gradually decreased during fermentation. Compared to methanol extracts of kochujang, water extracts and ethanol extracts showed higher free radical scavenging abilities at the initial stage of fermentation. Control, and samples with 0.5% (w/w) maesil powder or maesil concentrate, attained higher scores in sensory evaluation tests than did samples with higher maesil levels, and were not significantly different at P>0.05 as determined by Duncan's multiple range test.

Radical Scavenging Activities and Protective Effects against Oxidative Damage to DNA of Extracts from Medicinal Plants with known Osteoprotective Effects (골질 보호 작용이 있는 수종 생약 추출액의 래디칼 소거능 및 DNA 보호 효과)

  • Choi, Sung-Sook;Yim, Dong-Sool;Lee, Sook-Yeon
    • Korean Journal of Pharmacognosy
    • /
    • v.40 no.2
    • /
    • pp.143-149
    • /
    • 2009
  • Among aging diseases, the most basic problem is a decrease in bone mineral density. Patients with osteoporosis are steadily increasing in the world. This study was investigated to show the relationship between osteoporotic effects and oxidative damage. Water extracts of 15 medicinal plants and ethanol extracts of 14 medicinal plants with known anti-osteoporotic effects, were tested for their radical scavenging activity using DPPH, ABTS, SRSA and FRAP assay. Water extract of Cornus officinalis, Rubus coreanus and ethanol extract of Rubus coreanus, Viscium album var. coloratum, Cimicifuga heracleifolia showed about 15-20 mg/g of total phenolic contents. Water extract of Cornus officinalis, Rubus coreanus and Epimedium koreanum showed high radical scavenging activity. Ethanol extract of Drynaria fortunei, Cornus officinalis, Rubus coreanus, Gentiana scabra and Astragalus membranaceus showed high radical scavenging activity. Water extract of Drynaria fortunei, Cornus officinalis, Nelumbo nucifera, Epimedium koreanum, and Gentiana scabra showed very strong protective effect against oxidative damage to DNA. These results suggest the correlation between anti-osteoporotic effects and antioxidative effects.

The Investigation of Detonation Characteristics of Ethylene Oxide Mixture by Using Incident Shock Tube Technique (입사 충격파관을 이용한 에틸렌 옥사이드 혼합물의 데토네이션 특성연구)

  • Moon, J.H.;Chung, J.D.;Kang, J.G.
    • Transactions of the Korean Society of Automotive Engineers
    • /
    • v.2 no.5
    • /
    • pp.121-134
    • /
    • 1994
  • Shock tube investigation of ethylene oxide-$0_{2}-N_{2}$ mixture have been performed to reveal detonation characteristics of the mixture in terms of detonation pressure and speed. Theoretical calculation of thermodynamic parameters at the Chapmann-Jouguet detonation of the mixture has been also performed. A comparision of the observed results with the calculated ones can lead us to predict the detonation parameters of ethylene oxide in an artificial air. In addition, we have observed ignition delay times of ethylene oxide mixtures. The best fit of the observed delay times to Arrhenius gas kinetic relation gives : ${\tau}=10^{-144}{e{xp}}(E_a/RT)[C_{2}H_{4}O]^{-4.8}[O_{2}]^{-12.4}[N_{2}]^{-14.1}$ $E_a=3.67kcal/mole$ The observed activation energy is markedly reduced, compared with the case of ethylene oxide diluted in Ar. It could be due to the factor that $N_2$ play a role as detonation promoter yielding very reactive NOx radicals.

  • PDF

Studies on Screening of Seaweed Extracts for Peroxynitrite and DPPH Radical Scavenging Activities (과산화아질산염과 DPPH 라디칼에 대한 해조추출물의 소거 활성 효과)

  • Lee, Hee-Jung;Kim, You-Ah;Park, Ki-Eui;Jung, Hyun-Ah;Yoo, Jong-Su;Ahn, Jong-Woong;Lee, Burm-Jong;Seo, Young-Wan
    • Ocean and Polar Research
    • /
    • v.26 no.1
    • /
    • pp.59-64
    • /
    • 2004
  • As a part of our search for novel antioxidants from the seaweeds, we have investigated radical scavenging effect for their crude extracts using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1), a peroxynitrite-generating species in vitro. Thirty-four seaweeds were screened for $ONOO^-$ and DPPH radical scavenging activities. A potent inhibitory effect against peroxynitrite generated by SIN-1 at $5{\mu}g/ml$ of methanol extracts was observed in order of Ishige okamurae(95.3%), Sargassum hemiphyllum(90.2%), Symphyocladia latiuscula(89.6%), Porphyra suborbiculata(86.7%), and Gelidium amamsii(85.9%), Also, a significant scavenging effect against direct authentic peroxynitrite was revekaled for methanol extracts of Ishige okamurae(66.2%) and Sargassum hemiphyllum(55.2%) and the acetone/methylene chloride(1:1) extract of Gigatina tenella (61.0%). In our measurement for evaluating the capacity to scavenge the stable free radical of DPPH, acetone/methylene chloride(1:1) extracts of Symphyocladia latiuscula, Gloiopeltis furcata, and Sargassum thunbergii and the methanol extract of Sargassum sp. showed an inhibitory potency of 85.8%, 82.8%, 74.1%, and 64.0%, respectively.

Antioxidant and Nitrite Scavenging Effect of Soybean, Meju and Doenjang (대두, 메주 및 된장의 항산화 효과와 아질산염 소거 효과)

  • 최계선;임선영;최재수
    • Journal of Life Science
    • /
    • v.8 no.5
    • /
    • pp.473-478
    • /
    • 1998
  • The radical scavenging activity of the methanol extracts was in the order of doenjang〉 meju〉 soybean. The metha-nol extracts were further fractionated with dichloromethane, ethyl acetate, butanol and water in sequence. Among the solvent fractions of the methanol extracts, the ethyl acetate fraction demonstrated high levels of radical scave-nging activity. On the other hands, the nitrite scavenging activity of the methanol extrats was in the order of doen-jang〉soybean〉meju. All fractions from doenjang were exhibited the strong nitrite scavenging effect. Especially, $H_2O$, EtOAc and BuOH fractions with 6gmg/10$m\ell$ concentration exhibited much stronger nitrite scavenging effect than that of L-ascorbic acid. The ethyl acetate fraction showed remarkable antioxidant and nitrite scavenging effects compared with the other fractions obtained from soybean, meju and doenjang. The ethyl acetate fraction of doenjang was fur-ther purified by repeated chromoatograpic methods and isolated three compounds. There are identified as genistin, dai-dzein and genistein on the basis of spectral data.

  • PDF

Screening of Peroxynitrite and DPPH Raoical Scavenging Activities from Salt Marsh Plants (염생식물로부터 Peroxynitrite와 DPPH 라디칼 소거 활성 검색)

  • 서영완;이희정;김유아;안종웅;이범종;문성기
    • KSBB Journal
    • /
    • v.19 no.1
    • /
    • pp.57-61
    • /
    • 2004
  • A peroxynitrite is formed when superoxide and nitric oxide exist at near eqimolar ratio in biological systems. Although not a free radical by chemical nature, peroxynitrite is a powerful oxidant having a wide array of tissue damaging effects ranging from lipid oxidation and inactivation of enzymes and ion channels through protein oxidation and nitration to inhibition of mitochondrial respiration. During our search for new antioxidizing components from natural resources, twenty salt marsh plants were screened for their ONOO and DPPH radical scavenging activities. Among them, methanol extract of Rosa rugosa, lxeris tamagawaensis, Erigeron annus, Tetragonia tetragonoides, Imperata cylindrica, and Suaeda japonica inhibited more than 85% of peroxynitrite produced by 3-morpholinsydnonimine (SIN-1) at a concentration of 5 $\mu\textrm{g}$/$m\ell$. In addition, Rosa rugosa, Artemisia capillaris, Erigeron annus and Ixeris tamagawaensis showed significant scavenging effect against DPPH (1,1-diphenyl-2-picrylhydrazyl radical).