• Korean Journal of Veterinary Pathology
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• v.6 no.1
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• pp.27-39
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• 2002

The purpose of this study was to investigate to protective effects against porcine epidemic diarrhea virus (PEDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24hrs and 36hrs after birth. six piglets administered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Clinical signs and gross, histopathological lesion and immunohistochemical findings were examined. The results obtained were as follows; 1. In clinical signs, piglets of the control group appeared the typical signs of severe watery diarrhea, depression and anorexia but piglets of the PEDV antiserum treated group recovered progressively. In mortality, control group showed 75%, but PEDV antiserum treated group showed 16.7%, respectively. 2. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, containing curdes of undigested milk in stomach. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 3. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 4. In immunohistochemical findings, piglets of the PEDV antiserum treated group showed more intensive in reaction for IgG and IgG than those of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of PEDV antiserum to piglets was effective in preventing PEDV infection and reduced their mortality.

• Journal of the Korea Convergence Society
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• v.8 no.9
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• pp.9-16
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• 2017

In this paper, sub-microwave oscillator sensor is proposed to non-invasively monitor the glucose concentration level of the human biological tissue by oscillation frequency variation. Inductive slot in the ground plane of the microstrip line is combined with the biological tissue, to realize the resonator as a part of the oscillator sensor. The phantom box mimicking the human tissue is introduced for simulation of the resonator which resonance frequency correspondingly shifts up on three step glucose concentration levels(0, 400, 800 mg/dL). Oscillator sensor circuit is fabricated as a prototype. Pig tissues instead of human is used. Oscillation frequency shift of about 14 MHz per glucose level of 400 mg/dL has been successfully measured around 1,100 MHz. This proves that the proposed sensor is applicable to a blood glucose sensor.

• The Journal of Korean Academy of Prosthodontics
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• v.51 no.3
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• pp.190-198
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• 2013

Purpose: The purposes of this study were to investigate osseointegration around zirconia implants which had machined or alumina sandblasted surface, and to compare the results with titanium implants. Materials and methods: The study was performed on the tibia of 6 pigs. Three types of implants were investigated: group T-titanium implant, group Z-machined zirconia implant, group ZS-alumina sandblasting treated zirconia implant. Zirconia implants were manufactured from yttria-stabilized tetragonal zirconia polycrystalline (Acucera Inc., Pocheon, Korea). A total of 36 implants were installed in pigs' tibias. After 1, 4 and 12 weeks of healing period, the periotest and the histomorphometric analysis were performed. The data were analyzed using one-way ANOVA and significance was assessed by the Scheffe test (${\alpha}=.05$). Results: In the measurement of surface roughness, highest Ra value was measured in group T with significant difference. No significant differences were found among groups regarding Periotest values. After 1 week, in comparison of bone to implant contact (BIC), group Z showed higher value with significant difference. In comparison of bone area (BA), group T and group Z showed higher value with significant difference than group ZS. After 4 weeks, in comparison of BIC, group T showed higher value with significant difference. Comparison of BA showed no significant difference among each implant. After 12 weeks, the highest mean BIC values were found in group T with significant difference. Group ZS showed higher BIC value with significant difference than group Z. In comparison of BA, group T and group ZS showed higher value with significant difference than group Z. Conclusion: Zirconia implant showed low levels of osseointegration in this experiment. Modification of surface structure should be taken into consideration in designing zirconia implants to improve the success rate.

• Korean Journal of Agricultural Science
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• v.38 no.1
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• pp.71-77
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• 2011

Pigs have anatomically and physiologically very similar to human and because of this, pigs are the possible xenotransplantation donors for human organs. PERVs (Porcine Endogenous Retroviruses) are known to be one of the possible obstacles for using porcine organs regardless of the immunological barriers. In order to understand the expression patterns of PERVs in Korean native pigs, we investigated PERV expressions in porcine liver, heart, spleen, and lung samples. After RNA extraction, two types of specific PERV envelope genes (ENV-A and ENV-B) were amplified using specific primers by RT-PCR. The results indicated that the variable PERV expressions were observed in inconsistent patterns among animals and tissues. The PERV expressions were verified with semi-quantitative real-time PCR with three replicates. Even though, these results confirm the previous findings that the PERVs were differentially expressed between animals and tissues. These results also give some valuable information for xenotransplantation when using the Korean native pigs as the organ donor.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.269-269
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• 1994

GABA shunt에 관여하는 두 가지 효소인, GABA transminase와 Succinic semialdehyde reductase에 대한 단일 클론 항체를 생산하고 이들 항체의 특성을 살펴보았다. 소의 뇌에서 순수 정제된 효소를 동물에 주사한 후 immunodot-blot 분석법에 의하여 일차적으로 항체를 분비하는 hybridoma를 골라낸 후 생산된 단일 클론 항체가 특이적으로 이들 효소와 반응하는 가를 알아보기 위하여 Western blot 분석을 실시하였다. 뇌조직에서 추출한 총단백질을 SDS 전기영동법에 의하여 분리한 후 이들 항체를 처리한 결과, GABA-T에 대한 항체는 특이적으로 분자량이 50kDa에 해당하는 단백질 밴드만을 인지하였고 SSA reductase에 대한 항체의 경우 분자량이 34kDa 크기의 단백질 밴드와 반응하였다. 이들 분자량은 순수 정제된 소뇌의 효소 단백질의 크기에 해당하는 것을 확인하였다. 이들 소뇌의 효소에 대한 항체를 추적물체로 사용하여 다른 포유동물자 조류의 효소와 비교하는 cross-reactivity 연구를 수행하였다. 소, 돼지, 토끼, 쥐 (rat), 개, 고양이 그리고 닭의 뇌를 제거한 후 총단백질을 추출하고 Western blot을 해본 결과 GABA transaminase의 경우 조류를 제외하고 다른 포유동물에서는 같은 분자량의 단일 단백질 밴드를 확인하였고 SSA reductase의 경우 조류를 포함하는 모든 동물에서 같은 분자량의 밴드를 확인하였다 이상의 결과로 미루어 보아 포유류 뇌에 있는 이들 효소들은 변역학적으로 아주 유사하다고 사료된다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.5
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• pp.547-550
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• 2011

We have developed a laser-based needle-free liquid drug-injection device. A laser beam is focused inside the liquid contained in the rubber chamber of a micro-scale. The focused laser beam causes explosive bubble growth, and the sudden volume increase in a sealed chamber drives a microjet of liquid drug through the micronozzle. The exit diameter of a nozzle is less than 100 ${\mu}m$, and we verify that the injected microjet is fast enough to penetrate soft human tissue. In the experiment, the microjet penetrated a 5% gelatin-water solution that replicates the human thrombus and pork-fat tissue.

• Korean Journal of Veterinary Research
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• v.6 no.1
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• pp.1-9
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• 1966

From thse studies, the methodology of the complement fixation test for hog cholera has been described, and the application of the test for the diagnosis of hog cholera has been suggested.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.471-476
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• 2001

The effects of aging time (1,4 and 7 day), endpoint cooking temperature (70, 75 and 8$0^{\circ}C$) and cooking time (15, 30 and 45 sec) on the textural and sensory characteristics of pork loin were evaluated. As an aging period became longer, the pH, L＊ and b＊ value of raw meat became higher. As an aging period became longer, the cooking loss, hardness, chewiness and shear force value (SFV) of cooked meat became lower, and their tenderness and juiciness became better. However, no difference was observed in the aroma, and the one aged for 4 day among the cooked meat showed the best palatability. Increasing endpoint cooking temperature from 70 to 8$0^{\circ}C$ increased SFV and hardness and decreased chewiness, sensory tenderness and juiciness. Also, the pork cut with a thickness of 1.5 cm showed the best palatability when its internal temperature was 75$^{\circ}C$. And the pork cut with a thickness of 0.8 mm showed the best palatability when its cooking time was 30 sec.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.10
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• pp.210-212
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• 2014

The prostatic disease is one of common disease on andropathy. The prostatism, one disease of prostatic disease. is leaded a urination pain. To treat the this disease, Laser surgery is usually used with medicine treatment. Recently, the method of thermal therapy is rapidly increasing. Then we made the personal thermal treatment device for decreasing urination pain. And we have good performance data using pig skin.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.805-813
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• 1994

Immunohistochemical study on the intestinal tissues obtained from the 21 pigs of the 14 terms in Korea in which the clinical and epidemiological features had indicated the possible outbreaks of porcine epidemic diarrhea(PED) was performed using the indirect immunofluorescence test and/or the immunoperoxidase method in order to detect PED viral antigens in the infected cells of the intestines, and histopathological features were described as well. By immunohistochemical analysis, PED viral antigens were detected in the epithelial cells covering the small intestinal villi and recognized slightly in the cells lining the colonic surface epithelium as well. Occasional fluorescence was also seen in a few intestinal crypt epithelium. On light microscopy, the piglets with PED showed marked villous atrophy and fusion, and severe enterocyte degeneration and desquamation. On the other hand, the older pigs more than 4 week old age was mild villous atrophy and fusion, severe villous epithelial cell proliferation, and moderate mononuclear cell infiltration.