• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.499-502
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• 2007

The study was performed to assess the antinociceptive efficacy of Korean bee venom(BV) in the rat formalin test. Korean BV was collected using BV collector devices in which an electrical impulse is used to stimulate the worker bee(Apis mellifera L.) to sting and release venom. After collection, whole Korean BV was evaporated until dry using BV collector. Experiments were performed on male Sprague-Dawley rats(weighing 260-270, 6 weeks old). Rats divided into 4 groups, each comprising 8 rats. BV was diluted and amounts of 6 mg/kg body weight(BW), 0.6 mg/kg BW and 0.06 mg/kg BW were tested. BV was subcutaneously injected to produce an antinociceptive effect and the antinociceptive efficacy was evaluated using a rat formalin test. BV was subcutaneously injected into an acupoint(Zusanli, ST36) at 15 min prior to 1% formalin($50{\mu}l$) injection. The antinociceptive effect observed during 60 min following formalin administration. BV produced antinociceptive efficacy from 10 to 60 min after formalin injection. The antinociceptive efficacy of Korean BV showed a dose-dependent response. These results suggest that Korean BV may be a suitable and preferred choice for antinociceptive efficacy in pain management.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.157-164
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• 2012

This study was conducted to investigate the effect of different farrowing space, narrow and wide, on the sow performances and piglet growth performances. The $1.5m^2$ of narrow farrowing space was determined as the size of farrowing crate. The $3.0m^2$ of wide space for farrowing sow was the same of farrowing pen to allow the behavior freedom of sow. Baby piglets in the wide farrowing space was protected from crushing of sow by installation of safety bar. The pregnant sows used in this study were stall-housed during gestation and moved to each farrowing spaces on 8 d before parturition. Feed intake, backfat thickness and body condition score of sow were not affected by both farrowing spaces. However, the changes in backfat thickness and body condition score between farrowing and weaning in wide farrowing space were lower (p<0.05) than in narrow farrowing space. The return to estrus of sow was remarkably decreased in wide farrowing space compared to sows in narrow farrowing space. The lower number of stillbirth and higher mortality of piglets were observed in wide farrowing space. From the results, although wide farrowing space could be practically acceptable in terms of sow performances, possible cause of mortality of piglets should be scrutinized through observation of piglet and sow behaviors.

• Journal of Environmental Impact Assessment
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• v.24 no.5
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• pp.532-548
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• 2015

The winter ecology of individual yellow-throated martens(Martes flavigula) intemperate region of Korea were studied through snow-tracking. The study was performed across 3 winter seasons, from January 2011 to February 2013. Total distance of 49.8km was snow tracked (comprising 13 snow-tracking routes) to determine winter foraging habits, general behavior and movement paths of solitary and small groups (1-6 individuals; $mean=2.9{\pm}1.6$) of yellow-throated martens. The martens in the current study were omnivorous, with their winter diet including 9 animal and 5 plant species. Yellow-throated martens searched for food near and under the fallen logs and branches, root plates of fallen trees, around the roots of growing trees, and in small holes in the ground. They also climbed trees to search inside the tree holes and vacant bird nests. Foraging activity was estimated to occur at a frequency of 1.20 times/km, while territory marking occurred 1.42 times/km on average. Of the 60 documented foraging activities we observed, 17 were successful (28.3%). Moving activity and territory marking mainly occurred along ridges, whereas foraging activity was recorded in valleys, slopes, and forest edges. To protect the habitat of this species, the entire forest should be preserved, including the valleys, slopes, and even forest edges as well as main ridges.

• Proceedings of the KSCN Conference
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• 2003.11a
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• pp.1051-1052
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• 2003

미취학 아동기는 생후 1년 동안의 급격한 성장 이후로부터 학령기가 되기 전까지 성장이 완만해지는 시기이다. 이 시기의 아동들은 혼자 먹는 행동을 익히게 되고 식품에 대한 기호가 형성되는 등 식생활에서도 뚜렷한 개인특성을 갖게되며 이 시기에 형성된 식습관은 성인까지 이어지게 된다. 이 시기에 영양부족에 따른 신체발달의 지연이나 영양과잉에 따른 비만과 같은 과다한 신체발달의 문제점이 지적되고 있어 올바른 식습관 형성은 신체발달과 밀접한 관련성이 있는 것으로 보여진다. 우리나라는 취약한 칼슘섭취 부족에 따라 골격건강에 대한 관심이 크게 증가하고 있는데, 모든 질환이 그러하듯이 골격질환도 치료보다는 예방적인 관리가 다양한 측면에서 효율적이다. 따라서 골격건강과 최적의 신체발달을 위한 식사인자는 매우 중요하며, 일생의 성장시기에 따라 서로 다른 인자들이 관여할 것으로 생각된다. 이에 본 연구에서는 성장이 이루어지면서 식품선택의 독립성이 확립되는 미취학 아동에 있어 신체와 골격 발달에 관련성이 있는 영양섭취 요인을 찾아봄으로써 골격과 신체 발달을 위한 올바른 식습관 형성에 필요한 기초자료를 제시하고자 하였다. 이에 46~84개월의 미취학 어린이 총 62명(남자 37명, 여자 25명)을 대상으로 어머니에 의한 설문조사를 실시하고 대상자의 신체계측(InBody, Biospace)과 손목의 골밀도(DEXA, Medilink)를 측정하였다. 설문지는 어린이와 부모의 일반사항, 어린이의 건강상태, 식습관 및 활동조사표와 24시간 회상법에 의한 식사조사표로 구성하였다. 식사섭취조사 결과는 CAN-Pro를 이용하여 영양소 섭취량을 분석하였으며, 모든 연구결과는 SAS program을 이용하여 통계 분석하였다. 연구결과를 요약하면 다음과 같다. 남녀 어린이의 평균 월령은 62.43$\pm$7.26 개월과 62.12$\pm$8.85개월이었다. 출생시 신장과 체중은 남자 어린이가 50.92$\pm$2.14 cm와 3.36$\pm$0.42kg, 여자 어린이는 각각 50.29$\pm$1.36cm와 3.32$\pm$0.39kg이었다. 현재 신장과 체중은 남자 111.65$\pm$5.44 cm와 19.60$\pm$3.52kg, 여자 109.04$\pm$5.04 cm와 18.67$\pm$2.81kg이었으며, 비만지수는 남녀 각각 -2.13$\pm$9.09%와 0.22$\pm$10.49%였다. 손목의 골밀도는 남녀 어린이 각각 0.25$\pm$0.04g/cm, 0.24$\pm$0.03g/$\textrm{cm}^2$이었다. 에너지 섭취량은 남녀 어린이 각각 영양권장량의 66.43$\pm$18.58%와 70.46$\pm$20.31% 수준이었으며, 남녀 어린이 모두 단백질(124.97$\pm$37.55%, 131.24$\pm$45.49%), 비타민B$_{6}$ (152.48$\pm$47.01%, 152.95$\pm$61.77%), 엽산(106.87$\pm$40.44%, 104.52$\pm$50.16%), 비타민 E (121.04$\pm$52.79%, 113.48$\pm$75.78%)를 제외한 영양소 섭취량이 권장량에 미달되는 섭취수준을 보였다. 특히 성장기에 중요한 칼슘도 남녀 어린이 각각 권장량의 62.21$\pm$39.46%와 70.07$\pm$34.52%로 낮게 섭취하고 있었다. 모든 영양소 섭취량은 남녀 어린이간에 유의한 차이가 없었다. 신체계측치와 영양섭취와의 관계에서 체중, 체수분량, 제지방량은 각각 동물성 철 섭취량과 유의한 부의 상관관계를 보였다(p<0.05, p<0.01, p<0.01). 골밀도와 영양섭취와의 관계에서 손목 골밀도는 식물성 칼슘 섭취량과 유의한 정의 상관관계를 보였고(p<0.05), 특히 위쪽 손목 골밀도는 동물성 단백질, 동물성 철 섭취량과 각각 유의한 부의 상관을 보였다(p<0.05, p<0.05). 이상의 연구결과를 종합할 때 성장이 진행되고 있는 미취학 아동에 있어 신체상태와 골밀도는 동물성 단백질, 식물성 칼슘, 동물성 철 섭취량과 상관성이 있는 것으로 나타남으로써 앞으로 이들 영양소의 섭취가 신체와 골격 성장에 어떠한 영향을 미치는가에 대한 보다 세부적인 연구가 이루어져야 할 것으로 생각된다.

• Development and Reproduction
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• v.15 no.2
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• pp.143-150
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• 2011

The objective of this study was to determine the effect of exercise on a hemiparkinsonian rat model. Nigrostriatal dopamine cell lesions were produced by injecting 6-hydroxydopamine at the left medial forebrain bundle of rats. In this study, the rats were divided into the following 4 groups: the control group without any exercise, experimental group I with aqua-exercise (Exp I), experimental group II with balance exercise (Exp II) and experimental group III with complex exercise (aqua-exercise+balance exercise; (Exp III)). Exercises were applied to all the experimental groups after the operation. In order to observe the dopaminergic cell loss, we assessed the level of tyrosine hydroxylase (TH) expression in the midbrain of rats, and performed the apomorphine-induced rotation test at postoperative days (PDs) 7, 14, and 21. Experimental groups had significantly higher TH-immunoreactivity and behavioral performance than the control group. However, there was no difference in TH-immunoreactivity and behavioral performance across the experimental groups. These results suggest that the application of aqua-exercise and balance exercise could suppress dopaminergic cell loss in the substantia nigra of rat brains and could increase behavioral recovery in hemiparkinsonian rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.8
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• pp.1190-1196
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• 2013

The purpose of this study was to investigate the mechanism and effects of different types of ginseng on memory improvement in an experimental rat model. In this study, SD rats were induced for memory deficits through scopolamine treatment (1 mg/kg, i.p.) then administrated with ginseng extract for 7 weeks. The rats were divided into five groups: saline (1 mL/kg, NC: negative control), white ginseng (300 mg/kg, WG), red ginseng (300 mg/kg, RG), black ginseng (300 mg/kg, BG), and scopolamine (1 mg/kg, PC: positive control). The step through latency of the BG and RG groups was significantly longer than the PC group in the retention trial of multiple trial passive avoidance test. In the spatial reference memory triads of the Morris water maze test, the latency time of BG and RG was significantly lower than the PC group. In addition, in the prove test, the time spent in the platform quadrant of BG and RG groups were significantly longer than the PC group. Brain choline acetyltransferase (ChAT) activities BG and RG groups significantly increased compared to other groups. On the other hand, the levels of malondialdehyde (MDA) were significantly lower in the BG and RG groups compared to other groups. These result suggested that black ginseng could be useful to enhance learning memory and cognitive function by regulation of cholinergic enzymes.

• Environmental Analysis Health and Toxicology
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• v.15 no.4
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• pp.131-137
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• 2000

The release of hazardous waste materials into the environment poses serious risks in humans and ecosystems. The risk assessment of environmental pollutants including hazardous chemicals requires a comprehensive measurement of hazard and exposure of the chemicals that can be achieved by toxicity evaluation using a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed . In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 bp encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 Up of mRNA was consistently amplified in all the treated samples including control There were no significant differences in the TH expression level regardless of treating concentrations (1∼5,000 ppb) and time (0∼48 hr) The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue - specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard ofvarious environmental pollutants.

• Korean Journal of Environment and Ecology
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• v.33 no.1
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• pp.9-15
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• 2019

Artiodactyla, which is an even-toed mammal, widely inhabits worldwide. In recent years, wild Artiodactyla species have attracted public attention due to the rapid increase of crop damage and road-kill caused by wild Artiodactyla such as water deer and wild boar and the decrease of some species such as long-tailed goral and musk deer. In spite of such public attention, however, there have been few studies on Artiodactyla in Korea, and no studies have focused on the trend analysis of Artiodactyla, making it difficult to understand actual problems. Many recent studies on trend used text-mining and co-occurrence analysis to increase objectivity in the classification of research subjects by extracting keywords appearing in literature and quantifying relevance between words. In this study, we analyzed texts from research articles of three countries (Korea, China, and Japan) through text-mining and co-occurrence analysis and compared the research subjects in each country. We extracted 199 words from 665 articles related to Artiodactyla of three countries through text-mining. Three word-clusters were formed as a result of co-occurrence analysis on extracted words. We determined that cluster1 was related to "habitat condition and ecology", cluster2 was related to "disease" and cluster3 was related to "conservation genetics and molecular ecology". The results of comparing the rates of occurrence of each word clusters in each country showed that they were relatively even in China and Japan whereas Korea had a prevailing rate (69%) of cluster2 related to "disease". In the regression analysis on the number of words per year in each cluster, the number of words in both China and Japan increased evenly by year in each cluster while the rate of increase of cluster2 was five times more than the other clusters in Korea. The results indicate that Korean researches on Artiodactyla tended to focus on diseases more than those in China and Japan, and few researchers considered other subjects including habitat characteristics, behavior and molecular ecology. In order to control the damage caused by Artiodactyla and to establish a reasonable policy for the protection of endangered species, it is necessary to accumulate basic ecological data by conducting researches on wild Artiodactyla more.

• Korean Journal of Medicinal Crop Science
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• v.24 no.5
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• pp.341-350
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• 2016

Background: Prenatal exposure to infectious and/or inflammatory insults can increase the risk of developing neuropsychiatric disorder such as bipolar disorder, autism, and schizophrenia later in life. We investigated whether Valeriana fauriei (VF) treatment alleviates prepulse inhibition (PPI) deficits and social interaction impairment induced by maternal immune activation (MIA). Methods and Results: Pregnant mice were exposed to polyriboinosinic-polyribocytidilic acid (5 mg/kg, viral infection mimic) on gestational day 9. The adolescent offspring received daily oral treatment with VF (100 mg/kg) and injections of clozapine (5 mg/kg) for 30 days starting on the postnatal day 35. The effects of VF extract treatment on behavioral activity impairment and protein expression were investigated using the PPI analysis, forced swim test (FST), open field test (OFT), social interaction test (SIT), and immunohistochemistry. The MIA-induced offspring showed deficits in the PPI, FST, OFT, and SIT compared to their non MIA-induced counterparts. Treatment with the VF extract significantly recovered the sensorimotor gating deficits and partially recovered the aggressive behavior observed in the SIT. The VF extract also reversed the downregulation of protein expression induced by MIA in the medial prefrontal cortex. Conclusions: Our results provide initial evidence of the fact that the VF extract could reverse MIA-induced behavioral impairment and prevent neurodevelopmental disorders such as schizophrenia.

• Applied Microscopy
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• v.38 no.4
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• pp.321-328
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• 2008

The main purpose of this study is to investigate the effects of electrolyzed reduced water (ERW) on blood components and electrolytes, as well as elected tissues on mice. The mice were supplied either tap water (control group) and ERW (experimental group) for two months. There were no significant different between two groups anatomically and physiologically. In the blood electrolyte study, the experimental group had less Na and BUN compared to the control group. In the blood component study, the experimental group had less neutrophiles and the control group had more lymphocytes. In histological study, no tissue changes were noticed in various organs, including the stomach, small intestine, heart, and liver tissues. In conclusion, ERW has no noticable side affects on blood and organ tissues, and might be safe to a living body.