• Development and Reproduction
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• v.10 no.2
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• pp.115-125
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• 2006

The present experiment was performed to confirm the effects of selenium on maturation and viability of mouse oocyte. Maturation of oocytes was observed by microscope, Germinal vesicle breakdown(GVBD) and polar body formation(PB) were confirmed at 2.5, 13 hours after in vitro culture. Viability of oocytes was observed by microscope. Normal and abnormal oocytes were distinguished by morphological change in vitro culture for 72 hours. Glutathione(GSH) content of collected oocytes from individual stage also was measured by glutathione assay using spectrophotometer. The results obtained were as follows; The low concentration of selenium($0.005\;{\mu}g/mL{\sim}0.5\;{\mu}g/mL$) increased the maturation rate of germinal vesicle(GV) oocytes to GVBD and PB oocytes. The high concentration of selenium($5\;{\mu}g/mL$) decreased the maturation rate. The low concentration of selenium increased the viability rate of PB oocytes. The high concentration of selenium did not affect the viability rate. The low concentration of selenium increased the GSH content in PB oocytes. The high concentration of selenium decreased GSH content. GSH content in PB oocyte was much higher than that in GVBD oocyte. The results indicate that the low concentration of selenium increases the maturation rate by helping quality elevation of oocyte and minimizing damages of oxidative stress generated from metabolism process. The low concentration of selenium also increases the viability rate by increasing GSH content.

• The Korean Journal of Food And Nutrition
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• v.23 no.1
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• pp.88-93
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• 2010

The principal objective of this study was to evaluate the physicochemical characteristics of brown rice Dasik prepared with different 5 levels(0, 25, 50, 75, 100%) of modified potato starch powder. We assessed the general compositions, Hunter's color values, the mechanical characteristics, and conducted a sensory evaluation analysis of brown rice Dasik at room temperature($20^{\circ}C$). The more modified potato starch powder composition was increased, the content of ash, protein and fat were significantly decreased(p<0.05). We noted that the luminance of samples were increased the more modified potato starch powder composition was increased, but in Hunter's a and b values were decreased. With regard to the mechanical properties of the Dasik samples, we noted significant differences in hardness, gumminess, chewiness but no differences in springiness, cohesiveness and adhesiveness were detected. The more modified potato starch powder composition was increased, the more the score of hardness, gumminess and chewiness were higher. The results of sensory evaluation showed that there were significant differences on the color, sweetness, mouth-feel, chewiness, overall quality of the Dasik samples(p<0.05). The score of brown rice Dasik with 50%(w/w) modified potato starch(BM2) in color, mouth-feel, chewiness, overall quality were significantly increased than those of other samples.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.2
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• pp.259-267
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• 2008

This in vitro study aimed to investigate the influence of flowable composite lining with different thicknesses on the marginal quality. One hundred twenty cavities, each preparated with proximal boxtype( 3mm of bucco-lingual width, 2mm of mesio-distal depth and gingival margin of 1mm supra-CEJ) were randomly dived into four groups. group I : Tetric ceram filling alone(control group) group II: 0.5mm thickness Tetric flow + Tetric ceram filling group III: 1.5mm thickness Tetric flow + Tetric ceram filling group IV: 2.5mm thickness Tetric flow + Tetric ceram filling The followings are the results: 1. Group II showed significantly less microleakage compared to group I(control group)(p<0.05). 2. There was no statistically significant difference between group III, IV and group I(p>0.05). 3. Group II showed significantly less microleakage compared to group III, IV(p<0.05). 4. There was microleakage more or less in all group. It was concluded that 0.5mm flowable composite lining improved cavity adaptation and marginal sealing.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.829-838
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• 2007

The effect of tumbling time(0, 1, and 2hr) on quality characteristics of cured-smoked pork retail cuts (Bolgi, Seolgit, Boseop, Dogani, Satae) of hind leg was investigated. Quality analyses indicated the retail cuts of pork hind leg are variable except for proximate composition. The Satae ham had the lowest (P<0.05) lightness (L) and highest redness (a). The Bolgi and Seolgit ham had higher hardness than the other hams. Tumbling time(0, 1, and 2hr) had no significant(P>0.05) effect on proximate composition, pH, color, texture properties, and sensory properties of ham. However tumbling decreased cooking loss for Satae ham tumbled for 2hr(P<0.05). Tumbling time was required for more than 2hr to improve quality and obtain maximum yield of retail cut hams. Further study is necessary to improve quality and obtain maximum yield of pork retail cuts of hind leg.

• Journal of Nutrition and Health
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• v.43 no.4
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• pp.351-356
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• 2010

This study was done to evaluate the effect of Ca source using fish (Tilapia mossambica) scales on the bone metabolism. Male Sprague-Dawley rats, 4 weeks of age, were fed low-calcium diet (0.15% Ca) for 2 weeks. The rats on the low-calcium diet were further assigned to one of following three groups for an additional 4 weeks: 1) Ca-depletion group (LoCa) given 0.15% Ca diet ($CaCO_3$), 2) Ca-repletion group (AdCa) given 0.5% Ca diet ($CaCO_3$), 3) Ca-repletion diet (AdFa) received 0.5% Ca diet (Ca source from Tilapia mossambica scales). Serum parathyroid (PTH) and calcitonin showed no differences among experimental groups. Whereas LoCa group elevated the turnover markers, serum ALP and osteocalcin, and urinary deoxypyridinoline (DPD), AdCa and AdFa groups reduced their values. Elevation in the femoral weight, ash and Ca contents was observed in AdCa and AdFa groups. Bone mineral density was increased in AdCa and AdFa groups by 25-26% compared with LoCa group. These data demonstrate that Ca repletion with either Ca source from Tilapia mossambica scales or $CaCO_3$ is similarly effective in the improvement of bone turnover markers and BMD, suggesting the usefulness of Tilapia mossambica scales in the prevention of bone loss compared with $CaCO_3$.

• Korean Journal of Plant Resources
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• v.31 no.2
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• pp.109-116
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• 2018

Erectile dysfunction (ED), also known as impotence, is the inability to attain and sustain an erection firm enough to have sexual intercourse. Frequent ED may be a symptom of health problems including heart disease, obesity, alcoholism, stress, smoking, and depression, that need treatment. This study aimed to effect of complex extract (CPL) including Cornus officinalis on sexual function factor in the erectile dysfunction rat model. The erectile dysfuction rat model was induced by cimetidine (500 mg/kg in 5% ethanol, oral injection 2 weeks). Rats were oral administered with different concentration of CPL in rat erectile dysfunction model. As a results, sexual function factors (NO, cGMP) significantly improved in CPL treated groups (CPL-300, 600, 900 mg/kg) compared to CON group. Serum testosterone was increased in a dose-dependent manner after CPL treatment. Furthermore, administrations of CPL restored lumen areas of the prostate in the erectile dysfunction rat model. These results indicated that CPL alleviated erectile dysfunction by increasing sexual function factor and testosterone in rat model. CPL could be used to natural treatement for erectile dysfunction. However, further study is required to identify active ingredient and its mechanism of erectile dysfunction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.623-629
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• 2001

This study was investigated the quality characteristics of sponge cakes which were prepared b replacing 30% of flour with raw starch and resistant starches, such as, 4 cycled autoclaved-cooled RS 3 maize starch(RS 3), cross-linked RS 4 maize starch (RS 4) was superior to control cake( 100% flour cake). Addition of starches except RS 3 made crust and crumb color lighter and decreased hardness. Especially, cake containing RS 4 had the softest texture. For the adhesiveness and cohesiveness, cake containing RS 4 showed the least changes during 5 storage days. In DSC measurement, cakes containing RS 4 and ARS 4 showed high enthalpy in despite of low hardness. Sensory evaluation showed that cakes containing RS 4 and ARS 4 were significantly moist and soft compared to others. Overall acceptability of cakes containing starch, RS 4 and ARS 4 were evaluated excellent. Therefore, it can be suggested that RS 4 and ARS 4 can be added to the sponge cake for the quality improvement and functional element.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.10
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• pp.1229-1234
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• 2007

Antioxidant activities of extracts from anglerfish (Lophiiu Zitulon) were evaluated. Each part of fresh (skin, flesh, stomach, and liver) or dried (skin and flesh) anglerfish was extracted by four different solvents (methanol, ethanol, acetone, and distilled water). Antioxidant activities of the extracts were determined by radical scavenging activity (RSA) and reducing power (RP). Relatively higher RSA and RP were found in methanol and water extract of fresh anglerfish liver. Antigenotoxic effect of the extracts, which was measured by Comet assay, was shown in most of the extracts except methanol, acetone and distilled water extracts of fresh stomach sample. These results indicated that antioxidant and antigenotoxic properties of extracts from angler fish were variable depending on parts, solvent, and/or physicochemical state. The appropriate extraction process could provide some valuable bioactive materials from anglerfish.

• Korean Journal of Food Science and Technology
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• v.44 no.1
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• pp.135-139
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• 2012

This study was designed to evaluate the physicochemical properties and biological activities of steamed and fermented Codonopsis lanceolata. The treatments included NS-NF (non-steamed and non-fermented), NS-LF (non-steamed and L. rhamnosus fermented), S-NF (steamed and non-fermented), and S-LF (steamed and L. rhamnosus fermented). Total polyphenol amounts of S-NF and S-LF were significantly increased to more than 26 mg GAE/g. The highest DPPH scavenging activities were observed for S-NF and S-LF, showing $EC_{50}$ values of 0.8 and 0.6 mg/mL, respectively. The growths of Staphylococcus aureus, Listeria monocytogenes, Salmonella Typhimurium, and Shigella boydii were effectively inhibited by S-LF (MIC < 9 mg/mL). The NS-LF and S-LF ($EC_{50}$ <6 mg/mL) effectively inhibited ${\alpha}$-Glucosidase and tyrosinase activities compared to NS-NF ($EC_{50}$ <17 mg/mL). The S-LF exhibited the highest acetylcholinesterase (AChE) inhibitory activity ($IC_{50}$ <32 mg/mL). Therefore, the results suggest that the application of the steaming process combined with probiotic fermentation can effectively enhance the biological and pharmacological activities in C. lanceolata.

• Journal of Korean Ophthalmic Optics Society
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• v.16 no.3
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• pp.255-264
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• 2011

Purpose: To study the effect of hyaluronic acid(HA), which was widely used in artificial tears, multi-purpose solution(MPS), cosmetics, and drug acting on the musculo-skeletal system, in wettability and comfort of contact lens, we compared HA including lens(+HA) with HA excluding lens(-HA) by clinical evaluation. Methods: During four weeks, the selected 28 university student volunteers who did not have any eye disease wore +HA contact lens on their right eyes, and -HA contact lens on their left eyes, and then compared right eyes to left eyes as control. After prescribing by spherical equivalent calculation, over-refraction by auto-refractometer, and contact lens wearing for two weeks and four weeks, tear evaluation, the anterior of eyes examination, fitting convenience evaluation, surface wetting test, surveys were checked up. Results: Compared with the control group (-HA contact lens), +HA contact lens provided better wetting properties. The amount of released tear in -HA contact lens group became less in four weeks than before, but that in +HA contact lens group showed no significant difference from the initial one. In terms of the redness in eye, there was no significant difference between +HA and -HA contact lens groups. Fitting of +HA contact lens on eye was much easier than the control, -HA contact lens. From the results of survey, comfort, wettability, ease of cleaning, and dryness of eye in +HA contact lens group were generally better than those of -HA group, but redness and contact lens handling showed little difference. Conclusions: The addition of HA in soft contact lens(+HA) may enhance the wettability of lens during lens wearing, resulted in the better comfortable wearing of lens eventually.