• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.178-183
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• 2013

Traditionally, Codonopsis lanceolata root have been used as a source of natural heath food. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) and salicylic acid (SA) on adventitious growth C. lanceolata, the production of secondary metabolites, such as flavonoids, total phenolic compound, antioxidative activity (DPPH). The highest phenolics content was observed in treatment of 20 uM MeJA (74.53 mg/g). The content of total flavonoids followed the similar pattern as that of total phenolics, showing 38.45 mg/g of C. lanceolata treated by 20 uM MeJA. The DPPH scavenging activity was 24.2 ($IC_{50}$) of C. lanceolata treated by 20 uM MeJA. These results provide useful information for enhancing biological properties of cultural roots of C. lanceolata.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.165-170
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• 2014

The stems of Viburnum erosum were extracted with 80% MeOH. The concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated $SiO_2$, octadecyl silica gel, and Sephadex LH-20 column chromatographies. Based on NMR, MS, and IR spectroscopic data, the chemical structures were determined as betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-${\beta}$-D-glucopyranoside (3), and byzantionoside B (4). All the compounds were isolated for the first time from the stems of Viburnum erosum.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.73-80
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• 2024

To determinate the content of acanthoic acid and kaurenoic acid in 70% EtOH and hot water extracts of Eleutherococcus gracilistylus, quantitative analysis of each compound in samples was carried out by a HPLC-UVD. Also, the identification of each acompound in samples was successfully assigned by LC-MS analysis. In result, the contents of acanthoic acid and kaurenoic acid in 70% ethanoic extracts were 28.84±0.21 mg/g (2.88%), 26.38±1.63 mg/g (2.64%), respectively. However, the content of two compounds in hot-water extracts was not observed. In conclusion, it shows that 70% ethanol as a best extraction solvent to extract the acanthoic acid and its metabolite from Eleutherococcus gracilistylus was better than hot-water solvent. The 70% ethanol complex extract of Allium Hookeri and Eleutherococcus gracilistylus showed better effectiveness. In addition, the 70% ethanol extract complex of Allium Hookeri and Eleutherococcus gracilistylus showed better effects than the hot water solvent of DPPH radical scavenging ability, total polyphenols, and flavonoids content. The anti-inflammatory activity were significantly or partially reduced by treatment with ethanol extract complex(SEC) by Allium Hookeri and Eleutherococcus gracilistylus.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.228-235
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• 2014

The residue levels of flonicamid and its metabolites, 4-(trifluoromethyl)nicotinic acid (TFNA) and N-4-(trifluoromethyl)nicotinoyl glycine (TFNG) in sweer pepper were investigated to examine the residual characteristics of analytes for 87 days after pesticide application. The pesticide was applied once at recommended dosage and double dosage by foliar sprays and the samples of fruits and leaves of sweet pepper were collected for each treatment. The residues of flonicamid in all of fruits and leaves decreased gradually over time, while the residue levels of TFNG metabolite exhibited tendency that increased for long periods and thereafter decreased. Total flonicamid residual concentrations containing metabolites residues in fruit samples increased consistently until 30 days post-application and higher residue levels than residues at 1 day post-application were detected from 30 day to 87 day after treatment. The residue pattern observed in fruit could be explained by the movement of TFNG from leaves to fruits of plant. Such residual characteristic was similarly found in samples treated both recommended dosage and double dosage.

• Korean Journal of Environmental Agriculture
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• v.4 no.1
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• pp.43-51
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• 1985

To identify amino acid conjugates of indole-3-acetic acid(IAA) in plants, 23 amino acid conjugates of IAA were synthesized and characterized by UV and IR spectroscopies, and thinlayer and high performance liquid chromatographies. In etiolated pea(Pisum sativum L. var. Sparkle) shoots, aspartic and glutamic acid conjugates of IAA were tentatively identified as metabolites of endogenous IAA by thin-layer and high performance liquid chromatography, and by alkaline hydrolysis of the conjugates.

• Journal of Sericultural and Entomological Science
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• v.38 no.2
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• pp.186-188
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• 1996

곤충애 있어서 호르몬의 작용기작과 그 이용에 관하여 요야하면 다음과 같다. 곤충호르몬에는중추신경계의 신경분비세포에서 합성, 분비되는 peptide의 neuropeptide hormone (PTTH, bombyxin, diapause hormone등)이 있고, 상피계의 내분비선에서 합성, 분비되는 sesquiterpene의 유약호르몬 ( juvenile hormone)과 steroid의 탈피호르몬 (ecdysone)이 있다. 곤충호르몬은 특정한 표족세포에 있는 수용체와 높은 특이성과 높은 친화성을 가지고 결합해서 세포의 작용을 조절한다. 일반적으로 peptide hormone은 표적세포의의 세포막을 통과할 수 없으므로 표적세포의 막표면에 있는 수용체와 결합하는 것에 의해 세포내 대사제를 활성화시킴으로서 peptide hormone의 특이적인 발현이되는 것으로 알려지고 있다. 한편, ecdysone과 같은 steriod hormone이나 juvenlie hormone은 표적세포의 세포막을 용이하게 통과할수 있으므로 세포내의 세포막을 용이하게 통과할 수 있으므로 세포내로 들어가 수용체와 결합해서 hormone-receptor comlpex는 핵내로 들어가 DNA의 특이적인 영역에 결합하므로서 이들 호르몬 특이적인 기능이 발현되는 것으로 알려져 있다. Ecodysone의 활성이 있는 ecdysteroid가 여러 식물에서 발견되고 있어, 금후 양잠의 상족에 이용이 기대되고 있다. 또한, 향유약호르몬(AJH) 활성물지인 imidazole계 화합물은 양잠에 있어서 세섬도고치 생산에 그 이용이 기대되고 있다.

• Korean Journal of Pharmacognosy
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• v.5 no.2
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• pp.103-109
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• 1974

미국인삼(美國人蔘) (오가과(五加科), Panax quinquefolium L.) 2년생(年生) 및 4년생(年生) 식물(植物)에 심지법(法)을 사용(使用)하여 $squalene-H^3$를 투여(投與)했을 때 미국인삼(美國人蔘) 사포닌(panaquilins)과 그 비당체(非糖禮)에 $H^3-incorporation$ 가능성(可能性) 여부(與否)를 검토 하였다. $squalene-H^3$ 섭취율은 매우 낮은 값을 보였으며 $(40{\sim}86%)$, $squalene-H^3$는 또한 panaquilin의 비당체(非糖體) 부분(部分)에는 incorporation 되지는 않았으나 당체(糖體)에는 squalene 대사물질(代謝物質)이 incorporation된 것으로 사료(思料) 되었다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.1015-1018
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• 1998

Capsaicin(8-methyl-N-vanillyl-6-nonenamide: CAP) known well as a major compound of not taste in hot pepper, was investigated for the inhibition effect on in vitro nitrosation. CAP(100$\mu$mol) inhibited the formation of N-nitrosoproline(NPRO) and N-nitrosothioproline(NTPRO) by 56% and 26%, respectively. Vanillyl alcohol inhibited the nitrosation of proline by a concentration-dependent manner, and vanillic acid and vanillin were less effective in blocking the nitrosation of proline compared to CAP and anillyl alcohol. The inhibitory effect of NPRO formation by CAP was evaluated to similar with alpha-tocopherol, and vanillyl alcohol was more effective than alpha-tocopherol in blocking the nitrosation of proline. Our results suggested that CAP and its metabolites such as vanillyl alcohol could inhibit endogenous nitrosation in hydrophobic biological environment.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.83-91
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• 2018

Advances in bacterial and fungal genome mining uncover a plethora of cryptic secondary metabolite biosynthetic gene clusters. Guided by the genome information, targeted transcriptional derepression could be employed to determine the product of a cryptic gene cluster and to explore its biological role. Monascus spp. are food grade filamentous fungi popular in eastern Asia and several genome data belong to them are now available. We achieved transcription activation of a cryptic fungal polyketide synthase-nonribosomal peptide synthase gene Mpfus1 in Monascus purpureus ${\Delta}MpPKS5$ by inserting Aspergillus gpdA promoter at the upstream of Mpfus1 through double crossover gene replacement. The gene cluster with Mpfus1 show a high similarity to those for the biosynthesis of conjugated polyene derivatives with 2-pyrrolidone ring and the mycotoxin fusarin is the representative member of this group. The ${\Delta}MpPKS5$ is incapable of producing azaphilone pigment, providing an excellent background to identify chromogenic and UV-absorbing compounds. Activation of Mpfus1 resulted in a yellow hue on mycelia and its methanol extract exhibit a maximum absorption at 365 nm. HPLC analysis of the organic extracts indicated the presence of a variety of yellow compounds in the extract. This implies that the product of MpFus1 is metabolically or chemically unstable. LC-MS analysis guided us to predict the MpFus1 product and to propose that the Mpfus1-containing gene cluster encode the biosynthesis of a desmethyl analogue of fusarin. This study showcases the genome mining in Monascus and the possibility to unveil new biological activities embedded in it.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.391-397
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• 2016

Clostridium is a genus of Gram-positive, rod shape, spore-forming obligate anaerobe. Recently, Clostridium has been attracted as a host for bio-based chemical production, due to its diversity of substrate utilization and the production ability for metabolites which can be used as a building block for chemical production. Especially, butanol produced from Clostridium has been considered as an alternative fuel. As a transportation fuel, butanol has a higher energy density and lower hygroscopicity compared to ethanol, the first generation biofuel. Recently, metabolic engineering of Clostridium has been massively conducted for butanol production. In this study, the metabolic engineering strategy of Clostridium for butanol production has been reviewed with a brief perspective.