Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Identification of Secondary Metabolites from the Stems of Viburnum erosum

덜꿩나무(Viburnum erosum)줄기로부터 이차대사산물의 분리 및 동정

  • In, Seo-Ji (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Song, Na-Young (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Song, Myoung-Chong (Intellignet Synthetic Biology Center, KAIST) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Received : 2013.09.11
  • Accepted : 2013.12.10
  • Published : 2014.06.30
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Abstract

The stems of Viburnum erosum were extracted with 80% MeOH. The concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated $SiO_2$, octadecyl silica gel, and Sephadex LH-20 column chromatographies. Based on NMR, MS, and IR spectroscopic data, the chemical structures were determined as betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-${\beta}$-D-glucopyranoside (3), and byzantionoside B (4). All the compounds were isolated for the first time from the stems of Viburnum erosum.

덜꿩나무(Viburnum erosum) 줄기를 실온에서 80% MeOH 수용액으로 추출하고 농축하였다. 이 추출물을 EtOAC, n-BuOH, 및 $H_2O$ 분획으로 나누었다. 이 중 EtOAc및 n-BuOH 분획에 대하여 $SiO_2$, ODS 및 Sephadex LH-20 column chromatography를 반복 수행하여 4종의 화합물을 분리, 정제하였다. 분리한 화합물의 구조는 NMR, IR, 및 MS data를 해석하여, betulinic aldehyde (1), koaburside (2), (6R,7E,9R)-9-hydroxymegastigma-4,7-dien-3-one-9-O-${\beta}$-D-glucopyranoside (3), 그리고 byzantionoside B (4)로 구조를 결정하였다. 분리된 화합물 모두 덜꿩나무에서는 처음으로 분리되었다.

Keywords

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