• KOREAN POULTRY JOURNAL
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• v.33 no.11 s.385
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• pp.101-105
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• 2001

가금콜레라(Fowl Cholera)는 야생조류와 닭$\cdot$오리에서 급성 패혈증을 일으키고, 부검시 복강내 실질장기(심장, 근위, 십이지장)에서의 출혈을 일으킨다. 특히, 이 질병은 간의 다발성 괴사반점을 보이고 때로는 신경증상을 나타내는 것을 특징으로 하는 세균성 질병으로 제 2종 법정 가축전염병에 속한다. 국내에서는 지난해인 2000년 10월 22일부터 충청남도 서산시 소재의 천수만 간척지에서 야생조류의 집단 폐사가 발생했다. 그래서 국립수의과학검역원 조류질병과에서 현지 역학조사와 실험실 정밀검사를 통해 처음으로 폐사 원인을 가금콜레라로 진단 내렸다. 그 당시 본 질병으로 폐사한 야생조류는 대부분 오리류(가창오리, 청둥오리 등)였으며 총 13,000여수가 폐사 되었던 것으로 공식 집계되었다. 현재까지 국내에는 검역원 및 일선 시도 가축방역기관들의 철저한 방역조치와 함께 가금 사육농가의 위생적인 사양관리 등으로 닭이나 집오리에서의 가금콜레라는 발생되지 않고 있다.

• Korean Journal of Poultry Science
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• v.20 no.2
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• pp.65-72
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• 1993

The experiment was conducted to estasblish the large scale production of anti-serum against chicken IgG and to profile the developmental changes of serum IgG levels during all the feeding period(from hatching to 7 weeks of age) in broiler chicks. Blood sample were taken from Hubbard chicken hi-daily or /and weekly during the experimental period. The pure IgG was isolated from ammonium sulfate treated chicken serum by ion exchange chromatography. The quantitative assay of serum IgG were carried by RID method. It was observed that IgG concentrations were showed the highest at hatching(4.25 $m\ell$/$m\ell$), after that dicreased rapidly, lowest at 2 weeks of age(0.81 $m\ell$/$m\ell$), and gradually increased to 7 weeks of age (2.48 $m\ell$/$m\ell$) There was no differences of IgG level between sex, but the IgG levels of male chicks dicreased more rapidly and increased earlier thereafter than those of females during the first two weeks of agg.

• Korean Journal of Poultry Science
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• v.48 no.3
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• pp.101-110
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• 2021

In this study, we performed a cross-combination test using offspring from two paternal and five maternal parent stock lines to produce a new synthetic breed of Korean native commercial chickens. The survival rate, body weight, feed intake, and feed efficiency were measured for 10 crosses, with 837 Hanhyup-3 chickens used as a control. Most chickens had brown-based feathers, and there was little difference in color phenotypes among combinations. The average survival rate of the combinations was 94.9%, which was significantly different from that of Hanhyup-3 at 75.3% (P<0.01). There was significant differences in body weight among the combinations at all weeks of age (P<0.01). At 10 weeks of age, the FHHY combination was the heaviest with 2,300 g, while the weight of Hanhyup-3 was 1,922 g. In addition, the weight difference between the male and female chickens was 458 g. As a result of regression analysis, the duration needed to reach 1.9 kg was about 61 days for the FHFY, FHHY, and HFFY combinations, and 70 days for Hanhyup-3, exhibiting a 9-day difference. The average feed conversion ratio of the combinations until 10 weeks was 2.42, which was significantly different from that of Hanhyup-3 at 2.97 (P<0.01). In conclusion, FHHY, FHFY, and HFFY were determined to be the best new breed combinations based on production capacity. When these combinations are used as new commercial breeds, they are expected to increase the average weight by 18% and improve feed efficiency by 19%, as compared to the existing Hanhyup-3 breed.

• Journal of Life Science
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• v.31 no.12
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• pp.1079-1087
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• 2021

To analyze gut microbiota of livestock in Korea and compare taxonomic differences, we conducted 16S rRNA metagenomic analysis through next-generation sequencing. Fecal samples from broiler chickens, pigs, and cattle were collected from domestic feedlots randomly. α-diversity results showed that significant differences in estimated species richness estimates (Chao1 and ACE, Abundance-based coverage estimators) and species richness index (OUTs, Operational taxonomic units) were identified among the three groups. However, NPShannon, Shannon, and Simpson indices revealed that abundance and evenness of the species were statistically significant only for poultry (broiler chickens) and mammals (pigs and cattle). Firmicutes was the most predominant phylum in the three groups of fecal samples. Linear discriminant (LDA) effect size (LEfSe) analysis was conducted to reveal the ranking order of abundant taxa in each of the fecal samples. A size-effect over 2.0 on the logarithmic LDA score was used as a discriminative functional biomarker. As shown by the fecal analysis at the genus level, broiler chickens were characterized by the presence of Weissella and Lactobacillus, as well as pigs were characterized by the presence of provetella and cattele were characterized by the presence of Acinetobacter. A permutational multivariate analysis of variance (PERMANOVA) showed that differences of microbial clusters among three groups were significant at the confidence level. (p=0.001). This study provides basic data that could be useful in future research on microorganisms associated with performance growth, as well as in studies on the livestock gut microbiome to increase productivity in the domestic livestock industry.

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.151-156
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• 2007

The Function of the calcium sensing receptor (CaSR) is to control calcium levels by altering PTH (parathyroid hormone) secretion and renal calcium resorption. The influence of calcium on the basal and stimulated release of several hormones from chicken pituitary glands has been determined in vitro. The objective of this study was to identify SNP in chicken CaSR gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms within chicken CaSR gene. This study identified SNP at position 1949 bp(Genebank accession No : XM_416491) in the exon 1. The SNP changed the amino acid to alanine(GCC) from serine(TCC). This SNP showed three genotypes, AA, AS and SS by digestion with the restriction enzyme NcoⅠ using the PCR-RFLP method. The A963S showed significant effect only on the first lay day (P<0.05) in Leghorn population. Leghorn with the genotype AA had significantly faster the first lay day(137.6) than the genotype AS(143.0, P<0.05). Also, the A963S showed significant effect only on the first lay day(P<0.05) and mean of egg weight(P<0.05) in KNC population. KNC with the genotypes AA ans AS had significantly faster the first lay day (151.0 and 152.6, respectively) than the genotype SS(159.4, P<0.05). And the genotypes SS had significantly heavier the mean of egg weight(50.4 kg, P<0.05) than the genotype AA ans AS (47.5 and 47.8 kg, respectively). According to result of this study, an a allele of the A963S was found to have a significant effect on the first lay day. It will be possible to use this SNP marker on selecting chicken to improve the first lay day.

• Journal of Life Science
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• v.22 no.12
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• pp.1672-1679
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• 2012

The effect of high stocking density (HSD) on the expression of stress and lipid metabolism associated genes in the liver of broiler chickens was examined by chicken genome array analysis. The chickens in a control group were randomly assigned to a $495cm^2/bird$ stocking density, whereas the chickens in a HSD group were arranged in a $245cm^2/bird$ stocking density with feeding ad libitum for 35 days. The chickens assigned to the HSD group had a significantly lower body weight, weight gain, and feed intake compared with those of the control group (p<0.05). The mortality of chickens was higher in the HSD group than in the control group. The microarray analysis indicated up-regulation of stress associated genes such as HMGCR, $HSP90{\alpha}$, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, and down-regulation of interferon-${\gamma}$ and PDCD4 genes. The endoplasmic reticulum stress associated genes, HSPA5 (GRP78/Bip), DNAJC3 and ATF4, were highly expressed in the HSD group. The genes, ACSL5, TMEM195 and ELOVL6, involved in fatty acid synthesis, were elevated in the HSD group. The genes, ACAA1, ACOX1, EHHADH, LOC423347 and CPT1A, related to fatty acid oxidation, were also activated in the HSD group. These results suggest that a HSD rearing system stimulates the genes associated with fatty acid synthesis as well as fatty acid oxidation in the liver of broiler chickens.

• Korean Journal of Poultry Science
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• v.48 no.4
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• pp.193-206
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• 2021

This study aimed to identify the growth performance of Korean indigenous chicken pure-line by sex and twelve strains conserved in Poultry Research Institute, National Institute of Animal Science, Rural Development Administration. The effect of sex and strain on body weight was significantly different in every period, with males being heavier in all periods than females. In the case of biweekly weight gain, the tendency to increase rapidly from birth to six weeks old, and to decrease in the period from twelve to fourteen weeks old was common across all sex and strains. Depending on sex and strain, there were significant differences in age and the number of peaks. Regardless of sex and strain, the determination coefficient and adjusted determination coefficient showed high goodness of fit (99.1~99.9%) to growth functions. However, for each model, the goodness-of-fit had variations by sex and strains. von Betalanffy function had the best fit to growth curves in all the female strains except strain D. On the other hand, Gompertz function had the best fit for all the male strains except strain C. Logistic function showed the lowest goodness-of-fit in all sex and strains. Mature weights were in the order of von bertalanffy, Gompertz, and Logistic models, while growth ratio and maturing rate followed the order of logistic, gompertz, and von bertalanffy functions. This information could be useful for Korean indigenous chicken management and designing crossbreeding tests and breeding programs.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.06c
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• pp.383-386
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• 1998

한국어의 운율구조는 발화문장(utterance), 억양구(intonational phrase), 악센트구(accentual phrase), 음운적 어절(phonological word), 음절(syllable) 순의 계층적 구조를 가지고 있다. 본 연구에서는 운율구조의 각 층에서 성조 이벤트가 얹혀지는 음절이나 또는 각 층의 운율단위말의 음절의 음향적 길이를 측정함으로써 첫째, 운율단위말의 음절의 음향적 길이 또한 계층적 순위를 보이는지 둘째, 성조 이벤트(tonal event)와 음향적 길이 사이에 높은 상관관계를 보이는지 보고자 한다. 즉, 두 가지 측면에서 길이비교가 수행되었는데 하나는 언어 보편적 현상으로 알려진 구말 장음화 현상으로써 각 층 운율적 단위의 마지막 음절의 모음 길이 비교이며 다른 하나는 억양구초 고성조가 실현되는 음절의 모음과 어절 내 모음, 그리고 고성조가 실현되는 억양구말 음절의 모음간의 길이 비교이다. 남녀 각각 200문장의 각 분절음과 운율분석을 한 후 길이에 대한 일원분산분석 실시 결과 억양구말은 악센트구말 보다 길었으나 악센트구말은 어절말과 차이를 보이지 않거나 남자 화자의 경우 오히려 짧게 나타났다. 그리고 남자화자의 경우 악센트구초 고성자가 얹혀지는 음절의 길이는 어절 내 어절말 음절을 제외한 그 외 음절과 화자에 따라 큰 차이를 보이지 않거나 그보다 조금 짧게 실현되는 것으로 나타났다. 위의 결과는 첫째, 단위말 음절 모음의 장음화는 운율적 구조의 층위에 일대일 대응을 보이지 않는 것으로 해석되며 둘째, 성조 이벤트와 그것이 실현되는 분절음의 음향적 길이와는 큰 상관관계를 보이지 않는 것으로 해석될 수 있겠다. 그러나 이러한 일반화에 대한 충분한 근거 제공을 위해서는 해당음절의 모음 길이 뿐만 아니라 초성자음의 길이간의 비교와 음절자체의 길이 비교 또한 필요한 것이며 모음길이에 대한 선행자음의 분절음적 영향 고려가 수반되어야 할 것으로 보인다. 다음 내용을 정리해 보고자 한다.리해 보고자 한다.rc$ 구입할 때 중점적으로 살펴보는 사항은 신선도와 순수재래종 여부, 위생상태였다. 한편 소비자가 언제나 구입할 수 없다는 의견이 85.2%나 되어 원활한 공급과 시장조성이 아직 정착되지 않고 있었다. $\bigcirc$ 현재 유통되고 있는 재래종닭은 소비자 대부분이 잡종으로 인식하고 있었으며, 재래종과 일반육계와의 구별은 깃털색, 피부색, 정강이색등 외관상으로 구별하고 있었다. 체중에 대한 반응은 너무 작다는 의견이었고, 식품으로의 인식도는 비교적 고급식품으로 인식하고 있다. $\bigcirc$ 재래종닭고기의 브랜드화에 대한 견해는 젊고 소득이 높은 계층에서 브랜드화의 필요성을 강조하고 있다. $\bigcirc$ 재래종달걀의 소비형태는 대부분의 소비자가 좋아하였으나 아직 먹어보지 못한 응답자가 많았다. 재래종달걀의 맛에 대해서는 고소하고 독특하여 차별성을 느끼고 있었다. $\bigcirc$ 재래종달걀의 구입장소는 계란판매점(축협.농협), 슈퍼, 백화점, 재래닭 사육 농장등 다양하였으며 포장단위는 10개를 가장 선호하였고, 포장재료는 종이, 플라스틱, 짚의 순으로 좋아하였다. $\bigcirc$ 달걀의 가격은 200원정도를 적정하다고 하였으며, 크기는 (평균 52g)는 가장 적당하다고 인식하고 있으며, 난각색은 대부분의 응답자가 갈색을 선호하였다. $\bigcirc$ 재래종달걀의 구입시 애로사항은 믿을수 없고, 구입장소를 몰라서, 값이 싸다 등이었고, 앞으로 신뢰할 수 있고 위생적인 생산 및 유통체계가 확립될 경우 더 많이 소비하겠다는 의견이었다. $\bigcirc$ 재래닭 판매업소(식당)의 판매형태는 66.7%인 대부분의 업소가 잡종과 개량종 유색닭을 판매하고 있었으며, 1개 업소에서 1일 판

• Korean Journal of Poultry Science
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• v.41 no.3
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• pp.217-225
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• 2014

Telomeres are the ends of the eukaryotic chromosomes and consist of a tandem repetitive DNA sequence and shelterin protein complex. The function of telomere is to protect chromosome. Telomere length in somatic cells tends to decrease with organismal age due to the end replication problem. However, several factors at the genetic, epigenetic and environmental level affect telomere length. In this study, we estimated heritability of telomere length and investigated inheritance of telomeres in a chicken. Telomere length of lymphocytes was analyzed by semi-quantitative polymerase chain reaction using telomere primer and quantitative fluorescence in situ hybridization using telomeric DNA probe. In results, heritability of telomere length was estimated 0.9 at birth by offspring-parent regression analysis and was estimated 0.03 and 0.04 at 10 and 30 weeks old, respectively, by parental variance analysis. There was a significant positive correlation in telomere length between father and their offspring (r=0.348), and mother and their offspring (r=0.380). In inheritance patterns of telomere length, the influence of paternal and maternal effect on their offspring was similar. The influence of inherited telomeres on male and female progeny was also roughly alike. These results implicated that imprinting of parental telomere length was regulated by autosomal genes, not sex linked genes. In addition, telomere length of offspring at birth did not differ along with their maternal age. Thus, maternal age does not affects telomere length in their offspring at birth owing to cellular reprogramming at early embryonic stage.

• Korean Journal of Poultry Science
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• v.44 no.1
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• pp.19-28
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• 2017

The eggshell is an intricate and highly ordered structure composed of multiple layers and a calcified matrix. The eggshell is formed at the uterine segment of the chicken oviduct. In this study, histological changes in the uterine endometrium and the expression of the eggshell-related genes were investigated according to hen age. We analyzed the expression of eggshell protein-related genes, such as OCX-32, OCX-36, OC-17, OC-116, and eggshell-ion-related genes, such as CABL-1, SPP1, SCNN1G, ATP2A2, CA2, and CALM1. In chicken uterine endometrium, histological deformation, fibrosis, atrophy and elimination of micro-villi were found with increasing hen age. The concentration of blood-ion components did not significantly change with age. The amount of telomeric DNA in uterine endometrial cells decreased with increasing hen age. The expression of most of the eggshell-related genes changed significantly with increasing hen age. The expression of some ovo-proteins, which play a role in eggshell formation, increased with increasing hen age; however, there were no significant correlations among eggshell protein genes. Eggshell ion-related genes, such as ATP2A2, SCNN1G, CA2, and CALM1, were closely related to each other. The OCX-32 and OCX-36 genes were closely related to some of the eggshell ion genes. Eggshell protein-related genes, such as the OCX-32, OCX-36 genes and ion-related genes such as CALB-1, ATP2A2, SCNN1G, CA2, CALM1, affected eggshell formation, mutually or independently. This study shows that, uterine although endometrial cell damage occurs with increasing hen age, normal eggshells can be formed in old hens. This suggests that eggshell protein-and eggshell ion-related genes also control the homeostasis of eggshell formation.