• Korean journal of applied entomology
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• v.39 no.4
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• pp.211-226
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• 2000

Genetic divergence and phylogenetic relationships among the major Korean fireflies (Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa) were studied. A portion of mitochondrial COI (403 bp) and 165 rRNA (490~504 bp) genes were sequenced, and the GenBank-registered, homologous 165 rRNA sequences of Japanese fireflies were compared (27 species of Lampyridae, one of Lycidae, and one of Rhgophthalmidae). Greatest DNA and/or amino acid sequence divergence was found when P rufa, belonging to Lampyrinae was compared with H. papariensis and L. lateralis, both belong-ing to Luciolinae, confirming the current taxonomic status of the species. In the PAUP and PHYLIP analyses with 165 rRNA data, grouping of the two geographic samples of H. papariensis with H. tsushimana validate the use of generic name, Hotaria. Nevertheless, lack of sister-group relationship of the two geographic samples of H. papariensis renders further investigation on this group . Although the Korean and Japanese L. lateralis formed a strong monophyletic group, a substantial genetic differentiation was detected between them (2.9% of 165 rRNA gene sequence divergence). Finally, the geographic samples of Korean p. rufa strongly formed a group with Japanese p. rufa, warranting the use of generic name, Pyrocoelia, but the genetic distance observed between the Cheju-Island individual and all others requires further investigation on this subject. Summarized, this study supports the current taxonomic status of the Korean fireflies in that each respectively formed a strong monophyletic group with its own species or genus.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.181-191
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• 2000

We have sequenced a portion of mitochondrial CO! gene (403 bp) of the firefly, Pyrocoelia rufa, to investigate genetic diversity within population, geographic variation, and phylogenetic relationships among haplotypes. A total of seven mtDNA haplotypes ranging in sequence divergence from 0.2% to 1.2% were obtained from 26 fireflies collected at four localities in Korea: Namhae, Pusan, Muju, and Yongin. The samples collected at the urban area, Pusan, were all fixed with one haplotype, differently those collected at the forest and/or agricultural areas. This appears to suggest that habitat fragmentation and population bottleneck caused by urbanization might have been severe in Pusan. On the other hand, from Muju known as the largest habitat and sanctuary for the firefly, four haplotypes with the maximum sequence divergence of 1.0% were obtained, and this estimate was the highest among the areas studied. The fireflies collected at the isolated islet, Namhae, revealed relatively low haplotype diversity(H=0.25), but one haplotype (PR7) was phylogenetically differentiated from others. This phenomenon was explained in terms of biogeographic history of the island and gene flow in the recent past. Grouping of Muju- Y ongin and Pusan-Namhae, respectively, in the hierarchical genetic analysis suggests the presence of historically occurred, biogeographic barrier against gene flow between them.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.859-867
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• 2016

This study was conducted at rearing room to investigate the feeding behavior of fruits in the firefly (Pylocoelia rufa) larvae and the ovipositional characteristics of the adults. As a result, the pear, apple, melon, persimmon, mandarin, grape and peach were shown the feeding behavior of larvae the average 6.40, 3.25, 2.50, 2.20, 2.00, 1.40 and 1.25, respectively. However, the larvae were not shown at all feeding behavior to banana. The hours feeding behavior of firefly larvae was primarily from 8:00 pm until 2:00 am. In addition, the time at which the voracious feeding behavior was until 8:00 pm to 12:00 pm. The 6:00 pm began whisking all the larvae are hiding in the shelter. In the case of the firefly larvae fed only the edible snail, the adults were oviposition an average $49{\pm}6$ eggs. At that time larva stage, adults of the firefly eating the pears, apples, melon and eat edible snail at the same time there were oviposition the $53{\pm}7$ on average. In the case of fruits, the pears, apples, and melons has been considered worthy to take the firefly larva fed with the current land snails.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2001.10a
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• pp.64-64
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• 2001

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.05a
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• pp.144-144
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• 2002

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.99-99
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• 2001

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.94-94
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• 2002

• Food Science and Preservation
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• v.12 no.5
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• pp.482-488
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• 2005

Oxidant stress is well-known for a pivotal parameter related to neuro-inflammatory diseases including Alzheimer's disease, Parkinson's disease, and ALS (Lou Gehrig's disease). In order to effectively screen for anti-inflammatory agents, we first established the infrastructure of high throughput screening for anti-oxidant agents from medicinal insect library extracted with water, methanol, ethanol, and dimethyl sulfoxide. By the screening system, we found that Tenodera angustipennis Saussure, Pyrocoela rupa Olivier and Papilio maackii Mntris had strong anti-oxidant activity. Moreover, Tenodera angustipennis Saussure and Tenodera aridifolia (Stoll) exhibited protection effects of cellular damage by treatment of an oxidant hydrogen peroxide. Together, the results suggest that some selected hits could be a potential agent against neuro-inflammation, although the in vivo studies should be clearly tested.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.526-539
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• 2011

The purpose of this study is to identify insects inhabiting Mulyeongari-Oreum wetland and to provide basic information for conservation of wetlands. As a result, a total 229 species, 73 families, 9 orders of insects were collected: 136 species, 39 families, 6 orders from Mulyeongari-Oreum Wetland and 121 species, 52 families, 9 orders from Mt. Sooryeong. Of the 229 species of insects investigated, Copris tripartitus Waterhouse, an endangered species of wildlife fauna in Korea, was found with many individuals. Also, Lychnuris rufa(Olivier), having a narrow habitat near unpolluted water and mountains, was also recorded with 3 individuals and then should be considered as a protected species in Wetlands. To identify the community structure of moth on Mulyeongari-Oreum wetland, a light trap was examined and a total 76 species of moths were collected. Total dominant species was Hydrillodes morosa(Butler) with index of 0.12 and the species diversity on the moth community from survey area was the highest in July with index of 3.42.