• Korean journal of applied entomology
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• v.40 no.4
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• pp.345-348
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• 2001

We found that the cultivated oyster mushroom, Pleurotus ostreatus, was damaged by the phorid fly, Megaselia tamiladuensis Disney (Diptera: Phoridae). This fly is newly recorded as a pest in Korea. Brief morphological characteristics are described.

• The Korean Journal of Mycology
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• v.16 no.4
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• pp.210-213
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• 1988

Several reversion colonies were obtained after induced transfer of the isolated nuclei from P. sapidus into protoplast of P. ostreatus$({Arg}^-)$. These colonies showed three distinct cultural characteristics, type 1 produced spontaneous segregants of both parental types, type 2 showed segregants of non parental types, and type 3 gave rise to homogeneous colonies. Isozyme patterns of esterase, malate dehydrogenase and superoxide dismutase showed substantial differences between parents and nuclei transferred strains. This observation supported that the isolated nuclei of P. sapidus were transferred into protoplast of P. ostreatus and expressed in recipient cell.

• Korean journal of applied entomology
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• v.38 no.1
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• pp.41-46
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• 1999

This study was carried out to investigate the seasonal and regional occurrence and development of major pest flies on the oyster mushroom. The survey was performed in the oyster mushroom houses located at 16 places, selected randomly throughout South Korea from July to August and from October to November in 1997. The six species of flies were found including three pest species from Sciaridae, Cecidomyiidae and Scatopsidae flies. The pattern of occurrence was somewhat dissimilar depending on the survey periods and regions. Among these, the most serious and abundant pest, Lycoriella mali (Diptera: Sciaridae), occurred year around at the regions surveyed. In this study, Coboldia fuscipes (Diptera: Scatopsidae) was confinned as a mushroom pest. Its larvae damaged the mycelium, and occurred usually in summer from July to August. The paedogenetic Mycophila sp. (Diptera: Cecidomyiidae) was found abundantly on the oyster mushroom bed, and caused a severe damage in fall from October to November.

• The Korean Journal of Mycology
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• v.12 no.1
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• pp.15-19
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• 1984

Some factors affecting mycelial growth of Pleurotus sajor-caju and P. ostreatus. were investigated. The optimal temperature and pH of the medium for mycelial growth of both the species were $25^{\circ}C$ to $30^{\circ}C$, and 5 to 6, respectively. The effect of carbon and nitrogen sources on mycelial growth of the species was studied using modified Waksman's special medium as the basal medium. Generally, the disaccharides and polysaccharides showed good effect for mycelial growth of P. sajor-caju, and the polysaccharides were superior to the other classes of carbon sources for mycelial growth of P. ostreatus. Generally the organic nitrogen compounds showed good mycelial growth of both the species. The mycelial growth of the species were favored by the ammonium nitrogen more than by the nitrate nitrogen source. Asparagine was superior to the other kinds of amino acid. Six percentage of glucose and 0.6% ammonium tartarate was optimum for mycelial growth of P. sajor-caju whereas the optimum concentration for the P. ostreatut was 4% glucose and 0.4% ammonium tatarate.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.1
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• pp.49-55
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• 2020

This study was conducted to establish optimal high pressure liquid chromatography (HPLC) conditions for estimation of the ergothioneine contents in the three kinds of water extracts of Pleurotus eryngii var. ferulae (Meaksong, Beesan No. 2, Baekwhang). By comparing the four different HPLC conditions, optimun condition for quantifying the contents of ergotioneine was established (shodex HILIC column, 35 ℃, 1.0 mL/min). By this method, the contents of ergothioneine in Meakong (3.04 ± 0.02 mg/g), Beesan No. 2 (3.15 ± 0.05 mg/g) and Baewhang (1.13 ± 0.07 mg/g) were estimated. DPPH and ABTS radical scavenger activities of these three kinds of Pleurotus eryngii var. ferulae were estimated and the contents of total phenol and flavonoid were also estimated. Taken together, this study establish an optimun HPLC condition for determining the ergothioneine contents in water extracts of Pleurotus eryngii var. ferulae. Furthermore water extracts of Maesong and Beesan No. 2 showed relatively high contents of ergothioneine and antioxidant activity, suggesting that these materials could be used as potential antioxidant in developing functional cosmetics.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.39-43
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• 2011

A new cultivar "Dan Bi" of Pleurotus eryngii was developed by the method of mono-mono crossing between monokaryotic strains derived from KNR2312 and KNR2596. The parental strains, KNR2312 and KNR2596, are characterized by the property of high quality and a small number of primordia formation, respectively. The optimum temperature of mycelial growth was 25 and that of fruiting body development was $15{\sim}16^{\circ}C$. The period of harvesting including primordia formation was 0.7~1.3 days later than that of control strain Knneutari No. 3 in the culling cultivation. The color of pileus and stipe surface was neutral-brown and pure white, respectively. The shape of pileus was dome and has a scale like as cobweb. The yield was $93{\pm}9.7$ g per 850 cc of plastic bottle. Analysis of the genetic characteristics of the new commercial variety "Dan Bi" showed a different profile as that of the control strain, Knneutari No. 3, when RAPD (Random Amplified Polymorphic DNA) primer #8005 was used. This new variety "Dan Bi" of Pleurotus eryngii is characterized by a small number of primordia formation after scratching.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.108-108
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• 2019

느타리버섯은 전남 장흥군에서 병재배로 재배된 '곤지 7호' 느타리버섯 품종을 $3^{\circ}C$에서 1일간 냉각시킨 후 실험에 사용하였다. 이산화탄소 처리는 10mm 두께의 아크릴로 제작한 밀폐 챔버($40{\times}70{\times}40cm$)를 이용하여 CO2가스를 주입하여 내부의 CO2 농도를 $30{\pm}1%$로 조정하여 3시간 동안 밀폐시켰으며 $3^{\circ}C$ 저온 저장고에서 수행하였다. 처리 후 포장필름은 3처리로 하여 PVC랩, 재배농가에서 이용하는 필름인 $20{\mu}m$ OPP필름, $30{\mu}m$ OPP필름을 재료로 하여 산소투과율을 $5,000cc/m2{\cdot}day{\cdot}atm$로 조정한 필름(OPP5)을 사용하였다. PVC랩 처리는 스티로폼 트레이에 옮긴 후 균일하게 한 겹으로 둘렀고, OPP와 OPP5는 봉지에 옮겨 봉지 입구 4cm 안쪽을 비닐접착기로 열접착하여 밀봉하였으며 $3^{\circ}C$에 저장하면서 필름내부의 기체조성, 경도, 색도, 이취, 종합선도 변화를 조사하였다. 포장된 느타리버섯의 포장재 내 이산화탄소 농도는 저장초기 0.03%에서 점차 증가하여 저장 3일 후 PVC랩은 3.5~3.9%, 나머지 처리는 18%이상으로 증가하였으며 OPP필름보다 OPP5필름의 경우 포장재 내 이산화탄소 농도가 더 낮게 유지되었다. 생체중 감소는 PVC랩에서 저장 26일 후 9.9% 이하로 육안으로 보이는 시들음이 관찰되었으며 나머지 처리들은 저장 26일 후까지 1.6% 이하의 중량감소율을 보였다. 느타리버섯 갓 경도는 유지되었으며 줄기표면의 황색도 $b^*$값은 PVC랩에서는 증가 경향을, OPP나 OPP5에서는 유지 경향을 보였다. 느타리버섯의 이취 및 전체적인 품질은 무처리 후 PVC랩 포장은 저장 6일 후에 이취가 상품성 한계로 발생하였고 OPP5 포장은 포장재 중 가장 이취발생이 늦게 발생하였으며 CO2 처리에 의해 지연되는 것을 알 수 있었다. 이런 결과를 종합하여 느타리버섯의 $3^{\circ}C$ 저장 중 품질유지기간은 각 PVC랩(6일) ${\rightarrow}$ 30% CO2+PVC랩(7일) ${\rightarrow}$ OPP(10일) ${\rightarrow}$ 30% CO2+OPP(17일) ${\rightarrow}$ OPP5(20일) ${\rightarrow}$ 30% CO2+OPP5(22일)로 조사되었다. CO2처리 후 산소투과율을 $5,000cc/m2{\cdot}day{\cdot}atm$으로 조정한 미세천공(OPP5)필름이 가장 높은 전체적 품질점수를 나타내었으며 상대적으로 낮은 이취발생, 갈변과 갓무름이 적어 높은 점수를 얻는데 영향을 준 것으로 보인다.

• The Korean Journal of Mycology
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• v.20 no.2
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• pp.149-153
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• 1992

This study aimed to investigate possible cause of slow and abnormal growth of oyster mushrooms, Pleurotus florida and P. ostreatus collected from bad crop farms. Spherical virus particles of 30 nm in diameter from P. florida, 23 nm particles from P. ostreatus, and both 23 and 30 nm particles were also found from interspecies mated culture between P. florida and P. osreatus. The virus particles might be associated with the bad crop of Pleurotus species.

• The Korean Journal of Mycology
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• v.13 no.2
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• pp.75-78
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• 1985

Mutagenesis of protoplast could serve a great potential tool for improvement of strains and genetics in higher fungi. For the isolation of auxotrophic mutants from protoplasts of Pleurotus ostreatus and Pleurotus florida, viability levels of ultraviolet lights were determined. Seven auxotrophs were obtained from protoplasts irradiating UV to give $0.83{\sim}15%$ survival. The mutants showed a single requirement for each of Arg, Ribo-l, Ribo-2 or Phen for growth. Some of them showed two or three kinds of requirements, Gly Ser, Ade Hypo or Ala Om Tryp for growth.

• The Korean Journal of Mycology
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• v.24 no.3 s.78
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• pp.176-179
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• 1996

Media development of Pleurotus ostreatus were analyzed to recognize the compositional differences depending on rice straw and waste cotton stuff media. Yields of add the rice bran 20% to the waste cotton stuff were increment 11% as compared with rice straw media. Crude protein of products to the waste cotton stuff media on Pleurotus ostreatus was a much and crude ash was less than of rice straw media. Glutamic acid was most and cystine was lowest among the amino acids of Pleurotus ostreatus. Total amino acids and essential amino acids of waste cotton stuff were much than of rice straw media.