• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.239-244
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• 2005

This study was carried out to investigate the growth characteristics and root development of Mandshurian Linden (Tilia mandshurica Rupr. et Max.) seedlings grown in plastic net container and plain plastic container. The seedlings were grown in the mixture of peatmoss perlite : vermiculite (1:1:1, v/v/v) for five months in the plastic greenhouse. The seedlings grown in plastic net container showed greater root collar diameter growth, better root development, and more increment of root dry mass than those grown in the plain plastic container. In particular, the seedlings grown in plastic net container had no spiraling roots which were always observed in the control . These results suggest that the net container is suitable for container culture of Tilia mandshurica seedlings by enhancing root development. Seedlings grown in the net container and transplanted to various environments showed vigorous rootage and growth.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.123-129
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• 2000

This study was conducted to analyse the distribution of root-knot nematodes (Meloidogyne spp.) in fruit vegetable production areas in Korea. Soil samples were collected from greenhouses in Sungju (Kyungpook), Yeoju (Kyungki), Haman (Kyungnam), and Chungwon (Choongpook) provinces in 1997-1999. Plant parasitic nematodes were separated for density counting and some of the root-knot nematodes were identified using enzyme phenotypes of malate dehydrogenase (MDH) and esterase (EST). Among the 185 farms in Sungju province, Meloiciogyne spp. were detected from 99 farms (53.5%). Other plant parasitic nematodes detected were; Helic~otylmchuss pp. from 7 farms, Aphelenchus spp. from 43 farms, and Criconematids from 26 farms. Using the female enzyme phenotypes of MDH and EST. the four major root-knot nematodes in Korea, M. incognitc~(M I), M. uretznri~(M~ A), M. huplu (MH), and M. juvunica, could be identified. In the enzyme phenotype identification of 13 populations collected from Sunnam in Sungju province, 6 populations were identified as MA, 5 populations were identified as MI, and 2 populations were mixed with MI and MA. Among the 6 populations from Chojun in Sungju province. 4 populations were MA, one population was MI, and one population showed enzyme phenotypes of unknown species. Among the 14 populations of Yeoju province, l I populations were MH and 3 populations were MA.

• Journal of Korea Foresty Energy
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• v.21 no.3
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• pp.28-33
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• 2002

This study was carried out to develop the technique for the production of high quality container seedlings of Comus controversa Hemsley. The seedlings were grown on the medium of peatmoss : perlite : vermiculite (1:1:1, v/v) in plastic net container and no-net plastic container as control for five months in the PE house. The seedlings grown in the plastic net container showed better root collar diameter growth, better development of long and fine roots, more increment of dry mass of roots and shoots than those grown in the no-net container. But the height growth of the seedlings in both container was similar. In particular the seedlings grown in plastic net container had no spiraling roots which were always observed in the control container seedlings. This result was induced by air-root pruning effect from the plastic net container.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.295-303
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• 2019

This study investigated the effects of various cultivation pots on Betula platyphylla var. japonica seedlings in order to select suitable pots for the production of healthy seedlings for the ecological revegetation. We used six types of the pots natural pot, natural pot with inside 5 mm width root turning bump, natural pot with inside 10 mm width root turning bump, negative-phototropism pot, negative-phototropism pot with inside 5 mm width root turning bump, and negative-phototropism pot with inside 10 mm width root turning bump. As greenhouse cultivation provided a high level of control of irrigation and temperature, the seedlings did not show any significant differences in plant height, number of branches, and diameter at root collar, but showed a significant difference in root growth among the different types of pots. The root growth was best in the negative-phototropism pot. In the negative-phototropism, the roots grew in a vertical shape, while they grew abnormally in a spiral shape in the natural pots. In outdoor cultivation, the growth of seedlings showed significant differences according to container types. The seedlings grown in specially manufactured negative-phototropism pots were most excellent in all of the height, number of branches, diameter at root collar and root growth. As for the seedlings grown in the negative-phototropism pots, their roots grew vertically and thus their rooting was well established after they were transplanted outdoor. A size of less than 5 mm ridge installed in the negative-phototropism was found to be appropriate for the seedling growth. These results showed that the negative-phototropism pot would be best suitable for the growth of Betula platyphylla var. japonica seedlings and for the production of seedling for ecological revegetation.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.37-45
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• 2012

To select bacterial strains with antifungal activity against an anthracnose fungal disease causing damage severely on hot pepper, previous isolates obtained from plant root samples were screened. Among 457 isolates, IA70-5 isolate was finally selected and identified as Streptomyces padanus based on 16S rDNA sequence analysis. Strain IA70-5 is non-pigmenteous, non-mobile, and filamentous. S. padanus IA70-5 inhibited effectively the mycelium growth, spore germination, and appressorium formation of Colletotrichum acutatum in vitro. The results of this study demonstrated that IA70-5 strain, especially applied on fruit of hot pepper, decreased disease incidence 90% for pre-inoculation before pathogen treatment. Taken together, S. padanus IA70-5 strain is a promising biological control agent to control of a major fungal pepper disease, anthracnose.

• Korean Journal of Medicinal Crop Science
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• v.8 no.4
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• pp.334-341
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• 2000

This study was conducted to determine the effects and optimum concentrantion of chemical mutagens, colchicine, EMS (ethyl methan sulfonate), MNU (1-methyl-3-1­-nitrosoguanidinenitro), sodium azide $(NaN_3)$ for induction of mutant plants. In order to induce the mutants of Dianthus superbus L, immature seed were pre-soaked in the warter adding each mutagens and concentration of EMS, colchicine, MNU, and sodium azide $(NaN_3)$. Comparision of morphological characteristic and seed germination in each mutant plants differed depending on mutagen sources and their concentrations. When 0.2% EMS were treated on seed, germination decreased to 12% while untreated control was germinated 76.6% for twenty days. Treatments of colchicine appeared higher germination than other mutagen but not survived. The survival rate was extremely decreased in MNU treatment at 0.5mM and chlorophyll-mutant plantlets were obtained by sodium azide treatment at 0.2mM. Chlorophyll mutants were produced by pre-soaking the immature seed of Dianthus superbus L. with mutagen, sodium azide. The control plants appeared normal green leaf color, while mutant plant after mutagenic treatment of immature seed results in yellow­-green stripes and albino in normal green leaf tissue. RAPD was carried out to check the genetic modification of regenerated plants by mutagen treatments at 0.2mM sodium azide. Three polymorphic DNA fragments out of thirty-seven obtained by RAPDs were observed in regenerated plants using five decamer primers.

• Applied Microscopy
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• v.38 no.2
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• pp.117-124
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• 2008

Salvinia is an aquatic plant forming dimorphic leaves that have been modified into floating and submerged leaves. A air of floating leaves plays an important role for the floating and photosynthesis while the submerged leaves, which are lim and long, have the form and function of root. Many aquatic plants develop trichomes in the epidermis but in Salvinia, richomes grow densely in the epidermis of the dimorphic leaves. The present study examined the differentiation pattern of trichomes developing in the floating leaves of S. natans and S. molesta by scanning and transmission electron microscopy. Trichomes developing in the floating leaves of Salvinia showed very different patterns. In S. natans, they were arranged in a V-shape form, having 20${\sim}$25 rows at $18{\sim}25^{\circ}$ on both sides of the lamina divided by the midrib in the floating leaf. In each row, 8${\sim}$10 oval-shaped cells, $200{\sim}290{\mu}m$ in length, were arranged in a spiral fashion. Four trichomes of this form made a trichome unit, but their apical parts were separated from one another and developed into the so-called 'knuckle-crane' type. On the other hand, in S. molesta, trichomes differentiated in a unique pattern quite different from those of S. natans. At the early stage of differentiation, trichomes protruded from the epidermis and then 4${\sim}$6 cylindrical cells grew $400{\sim}600{\mu}m$ long and the four trichomes formed as an unit. The four grouped trichomes were interconnected through their apex and developed in the 'egg-beater' type. Then $300{\sim}600{\mu}m$ long multi-cellular stalk cells grew and protruded out of the epidermal surface from the basal part of the trichomes. Such a structural characteristic of trichomes is considered to play a very important role along with the aerenchyma tissue in the leaf mesophyll tissue for the floating of Salvinia on the water surface.