• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.753-762
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• 2008

The primers for RT-PCR and RACE-PCR were designed by aligning the pig genomic sequence and the human complement factor B(CFB) coding sequence(CDS) from the GenBank. Each PCR product was amplified in pig cDNA and sequencing was carried out. The CDS length of pig CFB gene was determined to be 2298 bp. In addition, the pig CDS was more longer than human and mouse orthologs because of insertion and deletion. The identities of porcine nucleotide sequences with those of human and mice were 84% and 80%, and the identities of amino acids were 79% to 77%, respectively. Three complement control protein(CCP) domains, one Von Willebrand factor A(VWFA) domain and a serine protease domain, that are revealed typically in mammals, were found in the pig CFB gene. Based on the CDSs determined, the primers were designed in intron regions for amplification of entire length of exons. In amplification and direct sequencing with genomic DNAs of six pig breeds, three cSNPs(coding single nucleotide polymorphisms) were identified and verified as missense mutations. Using the Multiplex-ARMS method, we genotyped and verified the mutations identified from direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS with two randomly selected DNA samples. The genotype of each sample exhibited the same results using both methods. Therefore, three cSNPs were identified from pig CFB gene and that can be used for haplotype analysis of the swine leukocyte antigen(SLA) class III region. Moreover, the results indicate that the Multiplex-ARMS method should be powerful for genotyping of genes in the SLA region.

• Journal of dental hygiene science
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• v.17 no.4
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• pp.283-289
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• 2017

The water discharged from dental unit waterlines (DUWLs) is heavily contaminated with bacteria. The development of efficient disinfectants is required to maintain good quality DUWL water. The purpose of this study was to establish a DUWL biofilm model using well-plates to confirm the effectiveness of disinfectants in the laboratory. Bacteria were obtained from the water discharged from DUWLs and incubated in R2A liquid medium for 10 days. The bacterial solution cultured for 10 days was made into stock and these stocks were incubated in R2A broth and batch mode for 5 days. Batch-cultured bacterial culture solution and polyurethane tubing sections were incubated in 12-well plates for 4 days. Biofilm accumulation was confirmed through plating on R2A solid medium. In addition, the thickness of the biofilm and the shape and distribution of the constituent bacteria were confirmed using confocal laser microscopy and scanning electron microscopy. The average accumulation of the cultured biofilm over 4 days amounted to $1.15{\times}10^7CFU/cm^2$. The biofilm was widely distributed on the inner surface of the polyurethane tubing and consisted of cocci, short-length rods and medium-length rods. The biofilm thickness ranged from $2{\mu}m$ to $7{\mu}m$. The DUWL biofilm model produced in this study can be used to develop disinfectants and study DUWL biofilm-forming bacteria.

• Journal of Korean Society for Geospatial Information Science
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• v.15 no.3
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• pp.19-26
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• 2007

The renewal update of the digital map constructed through NGIS has been conducted for the enhancement of usage, maintenance so that the latest data is guaranteed. The total update has been done every five years for five regions of the country using aerial photographs and satellite images. By launching the third phase of NGIS, the updating plan was changed from total to total or partial update including the real time update scheme. Furthermore, the update period was improved from five to two years and four years for a large and a small city, respectively. Therefore, it is necessary to develope a matching technique which combines various geographic information (such as drawing map, surveying drawing and map) with exact position on the digital map. In this study, we developed a matching algorithm based on central point and suggested a method which uses general surveying drawing for revision/update of the digital map. The general survey is conducted when a civilian development on lands are approved. Thus, the outcome from the survey, which is the general survey drawing, contains the latest various contents such as road, building, water pipe and manhole. A consistent and efficient method using the general survey drawing for near real time update of the digital map by applying the developed matching algorithm is presented.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.2
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• pp.30-39
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• 2015

The English Landscape garden and picturesque aesthetics, which was in fashion during the 18th to early 19th century in England, has been accused of making people see the actual garden in terms of a static landscape painting without a synesthetic engagement in nature. As new optic devices such as diorama, panorama, photography, and cinematography were invented, ways of seeing nature transitioned from a perspective vision to a panoramic, that is, modern one. This study intends to uncover signs of this kind of modern vision in the picturesque aesthetics and visual representation of landscape gardener Humphry Repton. German garden theorist Christian Cay Lorenz Hirschfeld contended that the English landscape garden was a new style of designing landscape that followed the principle of the serpentine line, which produced movement in sightlines; thus, he considered garden art as a superior art form among all other genres. The signs of visual motion appear in Repton's sketches of "Red Books". Firstly, he designed systemic routes in his clients' properties by considering different types of movements between walks and drives. Secondly, he often used the visual effects of panoramic views for his sketches in order to allow his clients to experience the human visual field. Lastly, he constructed sequences of sketches in order to provide his clients with an illusion of movement; in other words, Repton's sketches functioned as potential visual media to produce the duration of time in a visual experience. Thus, the garden aesthetics of the time reflected the contemporary visual culture, that is to say, a panoramic vision pertaining to visual motion.

• Journal of the Korean Institute of Landscape Architecture
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• v.38 no.2
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• pp.94-108
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• 2010

The purpose of this study is to provide data for the restoration of 'Yongyun(龍淵)' and 'Hwahongmun(華虹門)' through an investigation of the vicinity of their original landscapes at the time of construction of Hwaseong in Suwon and through tracing the transformative process of the environments of this vicinity. The results are as follows; As identified by 'Yongyunjung(龍淵亭)' and 'Yongdugak(龍頭閣)', other names of Yongyun, 'Banghwasuryujung(訪花隨柳亭)', which was built on 'Yongduam', is a facility whose place identity is highlighted with a sense of unity with Yongyun. The south lakefront of Yongyun, bordering Banghwasuryujung, has boundaries that make the best use of the natural geographical features of Yongduam while the current circumference of Yongyun is comparatively shorter than its original state. The size of 'Joongdo(中島)', however, seems to be an example of apparent over-design complement and reorganize 'Joongdo', which had been restored larger than its original state at the time of restoration in the 1970s. The depth of 'Yongyun' was created to be lower than the actual depth, without consideration for its initial depth, as soil was accumulated through continuous flooding after it was created. It is assumed that the original drains which were installed about 10m inside the lake were created facing the stream. As regards the planting environment, a circular planting of willows was made in the outskirts of 'Yongyun', except the 'Yongduam' which is a pure forest, and a mix of 'Pinus densiflora', shrubbery and deciduous broad leaf trees was planted in 'Joongdo'. Of the plants growing in the area of this study, plant species introduced to Korea after Hwaseong was constructed are found, most of which provide interest and attraction. The old pine trees growing in a group once grew in the castle areas of the vicinity even in the 1920s, the period of Japanese occupation, but they disappeared from the area in the aftermath of subsequent urban development and the Korean War. Although restored to the site, the number and space taken up by these trees are insignificant compared to those of the original environment. On the basis of these results, the following is considered necessary for the true restoration of the vicinity of 'Yongyun' and 'Hwahongmun': First, the grounds of 'Yongyun' should be dredged deeply enough to expose the bedrock and should be recreated in the rough outline of a half moon by extension to the southwest toward 'Yongduam' and 'Hwahongmun', and the size of 'Joongdo' should be significantly reduced. Secondly, considering that most plant species, except the pine trees and wild trees in 'Yongduam', are non-native plants introduced in order to provide such attractions such as the appreciation of scenic areas, they should be replaced with native species, mainly with the pine trees which were utilized during the construction of Hwaseong. The weeping willows planted in the 'middle-island' should be relocated to the outskirts of 'Yongyun', and replaced with pine trees as the major trees and maple trees or deciduous broadleaf trees to fill in the gaps. Thirdly, exotic species such as the 'Pinus rigida' planted in a group around 'Banghwasuryujung' and 'Bugammun' and 'Pinus strobus' planted in the vicinity of Hwahongmun' should be removed.

• The korean journal of orthodontics
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• v.18 no.2
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• pp.421-433
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• 1988

The purpose of this study was to investigate the characteristics of craniofacial morphology in adult with crowded dentition. The craniofacial morphology associated with normal and crowded of mandibular dentition was studied in lateral cephalometric radiograph obtained from 145 subjects grouped 44 males and 40 females in normal group, 33 males and 28 females in crowded group. The conclusions were as follows: Means, standard deviations and facial polygons from measurements were obtained from normal and crowded groups. Palatal plane to SN, occlusal plans to SN, mandibular plane to SN, mandibular plane to palatal plane and gonial angle measurements of crowded group were significantly different from normal group and were larger than normal group. SNA, SNB measurements of crowded group were significantly different from normal group and were smaller than normal group. Significant differences were observed between normal group and crowded groups on the basis of $SN/{\underline{1}}$, PP/1, $MP/{\bar{1}}$, and compared with normal group, upper incisors of crowded group were inclined more labially, whereas lower incisors of crowded group were inclined more lingually. Posterior facial height and ramal height were shorter in males of crowded group than those of normal group. Posterior facial height, mandibular length and posterior cranial base length were shorter in females of crowded group than those of normal group. Anterior facial height was longer in females of crowded group than those of normal group.

• Journal of Korea Water Resources Association
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• v.48 no.9
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• pp.729-741
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• 2015

This study examined the applicability of MBLRP (Modified Bartlett-Lewis Rectangular Pulse) rainfall generation model for an urban flood simulation which is a type of Poisson cluster rainfall generation model. This study constructed XP-SWMM model for Namgajwa area of Hongjecheon basin, which is a two-dimensional pipe network-surface flood simulation program and computed a flood discharge and a flooded area with input data of synthetic rainfall time series of 200 years that were generated by the MBLRP model. This study compared the data of flood with synthetic rainfall and flood with corresponding values which were based on design rainfall. The results showed that the flooded area computed with MBLRP model was somewhat smaller than the corresponding values on the basis of the design. A degree of underestimation was from 8% (5 year) to 34% (200 year) and the degree of underestimation increased as a return period increased. This study is meaningful in that it proposes methodology that enables quantifiability of uncertain variables which are related to a flooding through Monte Carlo analysis of urban flooding simulation and applicability and limitations thereof.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.4
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• pp.65-74
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• 2021

In order to efficiently inspect rapidly increasing old tunnels in many well-developed countries, many inspection methodologies have been proposed using imaging equipment and image processing. However, most of the existing methodologies evaluated their performance on a clean concrete surface with a limited area where other objects do not exist. Therefore, this paper proposes a 6-step framework for tunnel crack detection deep learning model development. The proposed method is mainly based on negative sample (non-crack object) training and Cascade Mask R-CNN. The proposed framework consists of six steps: searching for cracks in images captured from real tunnels, labeling cracks in pixel level, training a deep learning model, collecting non-crack objects, retraining the deep learning model with the collected non-crack objects, and constructing final training dataset. To implement the proposed framework, Cascade Mask R-CNN, an instance segmentation model, was trained with 1561 general crack images and 206 non-crack images. In order to examine the applicability of the trained model to the real-world tunnel crack detection, field testing is conducted on tunnel spans with a length of about 200m where electric wires and lights are prevalent. In the experimental result, the trained model showed 99% precision and 92% recall, which shows the excellent field applicability of the proposed framework.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.549-558
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• 2007

C4B and BAT2, assigned to the SLA class III region, were recently reported on relation with human diseases. The primers for RT-PCR and RACE-PCR for CDS analysis of these genes of pig were designed by aligning the CDSs of humans and mice from GenBank. After we amplified and sequenced with these primers and cDNAs, the full-length CDSs of pig were determined. The CDS lengths of C4B and BAT2 were shown as 5226 bp and 6501 bp. In addition, the identities of nucleotide sequences with human and mouse were 76% to 87%, and the identities of amino acids were 72% to 90%. After we carried out the alignment with determined CDSs in this study and pig genomic sequences from GenBank, the primers for cSNP detection in genome were designed in intron regions that flanked one or more exons. Then, we amplified and directly sequenced with genomic DNAs of six pig breeds. Four cSNPs from C4B and three 3 cSNPs from BAT2 were identified. In addition, amino acid substitution occurred in six cSNP positions except for C4248T of C4B. By the Multiplex-ARMS method, we genotyped seven cSNPs with DNA samples used for direct sequencing. We verified that this result was the same as that analyzed using direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS on two randomly selected DNA samples. The genotype of each sample showed the same result from both methods. Therefore, seven cSNPs were identified from C4B and BAT2 and could be used as the basic data for haplotype analysis of SLA class III region. Moreover, the Multiplex-ARMS method should be powerful for genotyping of genes assigned to the whole SLA region for the xenograft study.

• The korean journal of orthodontics
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• v.27 no.2
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• pp.221-230
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• 1997

It has been known that head posture may influence directly and/or indirectly the growth and development of craniofacial morphology and can also be influenced by the funtional demand of physiologic activity. It was reported that facial morphology has close relationships with hyoid bone position and head posture. In many previous studies, Natural Head Posture(NHP) was guided, and also it was shown that NHP has high degree of reproducibility. Otherwise, There was few study about the relationship of head posture, with routine cephalometric film which is used for clinical orthodontic purpose. In this study, according to the Wits and ANB of initial cephalometric film which was taken with vertical pendulum as representative of true vertical reference line. We classified the subjects which is comprised of 60 adult female patients into Class I, II, III (Cl I, II, III)and we tried to find out the correlation of head posture and hyoid bone position according to the classification of malocclusion. As a result of our research, we found the followigs. 1. In comparison of vertical position of hyoid bone relative to the cranial base. the position of hyoid bone of Cl III was lower than that of Cl II. 2. In comparison of anteriorpostes or position of hyoid bone, relative to the cervical column. The position of hyoid bone of Cl III was more anterior than that of a II 3. in comparison of vertical position of hyoid bone relative to mandible. There was no significant correlation aumoug the groups of malocclusion. 4. ANB and Wits showed no significant correlation with hyoid bone position. 5. The relative extension of head, which was noted in Cl II, showed negative with Sum, ANB. 6. In Cl II and Cl III, Post to Ant facial height showed positive correlation with NSL/VER.