• Korean Journal of Soil Science and Fertilizer
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• v.46 no.1
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• pp.40-48
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• 2013

This study evaluated the close crop recycling method by using the extract from defective onions. The mixture of the diluted extract (1/500) and nutrients was applied seven times on the onion leaves without spilling it onto the field. The yield and quality of the onions produced by this method (recycle) were compared to those grown organically (control) and conventionally (normal). The yield from the recycling field was increased significantly by 9% compared to that of the control field, while it was decreased by 11% compared to the normal field (p < 0.05). This lower yield was explained by the differences of the mulching vinyl and the fertilizers between the treatments which effect the onion growth during the winter. The content of quercetin in the onions from the recycling filed was increased significantly by 34% and 44% compared to those of the control and normal field, respectively (p < 0.05). It seemed that minerals and biologically active substances in the defective onion extract were effective in increasing the onion growth. In terms of soil microbial biomass, arbuscular mycorrhizal fungi was increased significantly in the recycle field by 40.1% and 30.6% compared to those of the control and normal fields, respectively (p < 0.05). On the other hand, microbial stress (cy19:0/18:1w7c) was decreased in the recycle field by 21.0% and 14.1% compared to those of the control and normal fields, respectively.

• Tuberculosis and Respiratory Diseases
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• v.45 no.2
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• pp.360-368
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• 1998

Background: Endothelin(ET), a potent vasoconstrictor peptide produced by endothelial cells and degraded predominantly in the pulmonary vasculature, have been implicated in the development of various organ dysfunctions. Plasma concentrations of ET-1 are reported to be elevated in patients with diffuse interstitial lung disease(DILD). But, there is no study to establish the exact source and mechanisms involved in the increased plasma ET-1 concentrations in DILD patients. Methods: 12 patients with IPF, 2 patients with sarcoidosis, 2 patients with scleroderma, 1 patient with SLE and 11 healthy volunteers were studied. ET was detected by radioimmunoassay in plasma and bronchoalveolar lavage fluid(BALF) as well as in 24-hr urine specimens. For each subjects, arterial/venous(A/V) ET ratio and renal ET clearance were calculated. Results: Elevated plasma, urine and BALF ET concentrations were found in patients with DILD compared with controls. But, no significant difference was observed in ET A/V ratio and ET renal clearance between patients with DILD and controls. Conclusion: We observed that plasma ET concentrations were elevated in patients with DILD, and that the main site of ET production may be lung parenchyme.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.202-208
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• 2006

Juice obtained from premature citrus fruits was deacidified by electrodialysis (ED), and its physicochemical and functional properties were evaluated. Total acidities decreased from 3.15 (Aug), 1.62 (Sep), and 1.45% (Oct) to 2.37 (Aug), 0.86 (Sep), and 0.74% (Oct) by electrodialysis for 100 min, respectively, whereas $^{\circ}Brix$ to acid ratio increased, and pH and color remained almost unchanged. Free sugar content of premature citrus juice remained almost unchanged, while organic acid content decreased significantly. Narirutin and hesperidin contents among flavonoids slightly, but not significantly, decreased. $K^+,\;PO_4\;^{2-},\;SO_42^-,\;and\;Cl^-$ contents decreased by ED, with $K^+$ content showing more than 60% decrease, while $Na^+$ content increased 2.5-3.5 times. Total polyphenol content and electron-donating ability decreased slightly by ED, while nitrite-scavenging ability remained almost constant. Sensory evaluation indicated juice (Sep) subjected to ED processing to $12^{\circ}Brix$ with sugar had the highest preference.

• Journal of Life Science
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• v.21 no.1
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• pp.96-101
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• 2011

S-Adenosyl-L-methionine synthtase (SAM-s) catalyzes the biosynthesis of SAM from ATP and L-methionine. SAM plays important roles in the primary and secondary metabolism of cells. A metK encoding a SAM-s was searched from Streptomyces natalensis producing natamycin, a predominantly a strong antifungal agent, inhibiting the growth of both yeasts and molds and preventing the formation of aflatoxin in filamentous fungi. To obtain the metK of S. natalensis, PCR using primers designed from the two highly conserved regions for metK genes of Streptomyces strains was carried out, and an intact 1.2-kb metK gene of S. natalensis was cloned by genomic Southern hybridization with PCR product as a probe. To identify the function of the cloned metK gene, it was inserted into pSET152ET for its high expression in the Streptomyces strain, and then introduced into S. lividans TK24 as a host by transconjugation using E. coli ET12567(pUZ8002). The high expression of metK in S. lividans TK24 induced actinorhodin production on R5 solid medium, and its amount in R4 liquid medium was 10-fold higher than that by exconjugant including only pSET152ET.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1357-1362
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• 2013

The objective of this study was to evaluate the effects of far-infrared (FIR) irradiation on the antioxidant and tyrosinase inhibitory activities of Schizonepeta (S.) tenuifolia. After FIR irradiation of S. tenuifolia for 30 min at 70, 90, or $110^{\circ}C$, water extracts (2 g/100 mL) were prepared at $60^{\circ}C$ for 1, 3, or 5 h. Total phenol content (TPC), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities (RSAs), and tyrosinase inhibitory activity of the extracts were determined. TPC, antioxidant and tyrosinase inhibitory activities of the extracts increased significantly with increasing FIR irradiation temperature of S. tenuifolia. For example, irradiation at $110^{\circ}C$ for 5 h increased TPC, DPPH RSA, ABTS RSA, and tyrosinase inhibitory activity of the extract from 204.64 to $618.67{\mu}g$ gallic acid equivalents/mL, from 30.15 to 80.84%, from 24.43 to 55.33%, and from 7.05 to 33.65%, respectively, compared to the extract of untreated S. tenuifolia. These results indicate that FIR irradiation may be useful as a processing method for enhancing the quality of S. tenuifolia.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.553-560
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• 2005

The stf (Salmonella typhimurium fimbriae) operon consisting of stfA(CDEFG assumes to encode putative fimbriae. The complete stf operon is existed in S. typhimurium and S. choleraesuis, whereas it is absent in S. typhi. Analyses of the amino acid residues between major subunit StfA of the Stf fimbriae and those of known other fimbriaes suggested that Stf belongs to class I type fimbriae. Through comparison of StfD chaperone with the other fimbrial chaperones, and of C-terminus in subunits of Stf fimbriae, it belongs to FGS (with a short Fl-G1 loop) subfamily. In order to investigate the expression of stf operon, we have constructed a Salmonella strain containing a chromosomal stfA::lacZYA transcriptional fusion, resulting in S. typhimurium $_X8532$. The strain $_X8532$ lacked the expression of \beta-galactosidase$ under normal culture conditions. However, with longer incubation time of the S. typhimurium $_X8532$, we have isolated 21 individual strains exhibiting $Lac^+$ phenotype. $Lac^+$ phenotype was appeared as approximately 0.03 frequency per generation. All isolates expressed lacZ constitutively in the various environmental conditions. Various global regulatory proteins including RpoS, OmpR, and CpxR were not involved in the regulation of the stf operon. A S. typhimurium $_X8661$ mutant lacking stfAC function attenuated 6.7 folds more than that of wild type $_X3761$ in the mouse virulence test, suggesting in the somehow involved in the Salmonella pathogenesis.

• Korean Journal of Heritage: History & Science
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• v.50 no.3
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• pp.126-145
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• 2017

The Seokgaejae section is located along a timber access road and a driveway across Bonghwa County, North Gyeongsang Province, and Samcheog city, Gangwon Province. Its stratigraphic column shows all of the ten strata of the lower Paleozoic Taebaek Group, Joseon Supergroup. The Seokgaejae section is proved to be scientifically important. Thirty-eight domestic/international journal papers have been published on this early Paleozoic stratigraphic site, and many distinguished researchers over the world have visited the site. However, the section has never been considered to be designated as a national natural monument and was not included in the adjacent Gangwon Paleozoic National Geopark due to management or administrative issues. Although the Seokgaejae section sufficiently satisfies many of the national natural monument criteria, designation for the natural monument may not be justified because of the facts that the outcrop was artificially exposed by road construction; the chance of destruction of the outcrop is relatively few; demage on the outcrop to some extent does not impair the intrinsic value of the section; and the geomorphological/landscape value of the section is low. The application of the recently modified geological heritage assessment model to the Seokgaejae section shows very high scores on the scientific/educational, intrinsic, and functional values. Based on the improved geological heritage grade standard, the Seokgaejae section conforms to the national-level protection criteria. It is strongly recommended to manage the Seokgaejae section as a principal geosite by including it in the Gangwon Paleozoic National Geopark. This case study on the Seokgaejae section also suggests that the process of application and endorsement of a national geopark need further improvement. As well as the improvement of the system or policy related to geological heritages, further efforts of the experts in various fields of geoscience are required in order for other geological heritages not to be neglected from now on.

• Journal of Navigation and Port Research
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• v.42 no.6
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• pp.421-426
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• 2018

With regard to LED lighting devices which have currently been commercialized, LED operating sequences are being sold in a fixed state. In such a state, the external environmental factors are not taken into consideration as only the illumination environment application is considered. Currently, it is difficult to create an optimal lighting environment which can adapt to changes in external environmental factors in the ship. Therefore, it was concluded that there is a need to input the external environment value so that the optimal illumination value can be reflected in real time in order to adapt more organically and actively to the change of external environmental factors. In this paper, we used a microprocessor as an integrated management system for environmental data that changes in real time according to existing external environmental factors. In addition, a controller capable of lighting control of RGB LED module by combining fuzzy inference system. For this, a fuzzy control algorithm is designed and a fuzzy control system is constructed. The distance and the illuminance value from the external environment element are input to the sensor, and these values are converted to the optimum illumination value through the fuzzy control algorithm, and are expressed through the dimming control of the RGB LED module and the practical effectiveness of the fuzzy control system is confirmed.

• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.304-312
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• 2021

The present study aimed to develop new physiologically active ingredients from Angelica gigas. The polysaccharides purified from A. gigas, AGE-2c-I, showed potent anti-complementary activity in a dose-dependent manner. C₃ activation products were identified through crossed immuno-electrophoresis using anti-human C₃ antibodies and the anti-complementary activity of AGE-2c-I under Ca⁺⁺-free conditions suggests that AGE-2c-I may induce complementary activation via both alternative and classical pathways. In addition, AGE-2c-I augmented the production of various cytokines, such as interleukin (IL)-6, IL-10, IL-12, and tumor necrosis factor-α, by peritoneal macrophages. Furthermore, intravenous (i.v.) administration of AGE-2c-I dose-dependently enhanced natural killer cell cytotoxicity against YAC-1 lymphoma. In experimental lung metastasis, prophylactic i.v. administration of AGE-2c-I inhibited lung metastasis by 58% at 100 ㎍/mouse. From the above results, we suggest that AGE-2c-I purified from A. gigas has potent immune system-stimulating activities, and is a potentially promising food ingredient beneficial to human health.

• Journal of the Korean Geosynthetics Society
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• v.21 no.4
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• pp.1-12
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• 2022

Generally, the plate load test and the field density test are conducted for compaction quality control in earthwork, and then additional analysis. Recently developed that the DCPT (Dynamic Cone Penetration Test) equipment for smart compaction quality control its the system are able to get location and real-time information about worker history management. The IoT-based the DCPT system improved the time-cost in the field compared traditional test, and the functions recording and storage of the DPI (Dynamic Cone Penetration Index) were automated. This paper describes using these DCPT equipment on in-situ and compared to the standards of the DCPT, and the compaction trend had be confirmed with DPI as the field test data. As a result, the DPI of the final compaction decreased by 1.4 times compared to the initial compaction, confirming the increase in the compaction strength of the subgrade compaction layer 10 to 14 cm deep from the surface. A trend of increasing compaction strength was observed. This showed a tendency to increase the compaction strength of the target DPI proposed by MnDOT and the results of the existing plate load test, but there was a difference in the increase rate. Therefore, additional studies are needed on domestic compaction materials and laboratory conditions for target DPI and correlation studies with the plate load tests. If this is reflected, it is suggested that DCPT will be widely used as smart construction equipment in earthworks.