• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.25-31
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• 2003

Delftia acidovorans 51-A isolated from river water degrades aniline. In order to clone genes involved in aniline degradation, transposon Tn5-B20 was inserted into the strain 51-A to generate a mutant strain 10-4-2 that cannot utilize aniline as a carbon source. The mutant strain was not an auxotroph but could not degrade aniline. Southern hybridization analysis indicated that the transposon was inserted into the mutant bacterial DNA as a single copy. Flanking DNA fragment of Tn5-B2O insertion was cloned and sequenced. DNA sequence analysis revealed three ORFs encoding TdnQ, TdnT, and TdnA 1 that arc responsible for catechol formation from aniline through oxidative deamination. The analysis also confirmed that Tn5-B2O was inserted at the immediate downstream of tdnA1. The result suggests that the transposon insertion behind tdirA1 disrupted the pathway of the catechol formation from aniline, resulting in the mutant phenotype, which cannot degrade aniline. A large plasmid over 100-kb in size was detected from D. acidovorans 51-A and Southern hybridization analysis with Tn5-B2O probe showed that the transposon was inserted on the plasmid named pTDN51. Our results indicated that the tdn genes on pTDN51 of D. acidovorans 51-A are involved in aniline degradation.

• The Journal of the Korea Contents Association
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• v.18 no.3
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• pp.114-123
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• 2018

Korea's Export Support Policy has shifted from conglomerate to SMEs since the 1998 IMF financial crisis. Therefore the SME export result in 2011 has reached the quantitative growth of more than US$ 100 billion for the first time. However, the trend has remained stagnant since 2013. Such a stagnant is judged to exist on the part of the Export Support Policies that fail to significantly enhance export competitiveness. Therefore, in order to expand the base of the export capabilities of SMEs and enhance the export competitiveness, the researcher has analyzed the problems of the Export Support Policy focused from the major prior studies since 2010 and derived the efficiency improvement methods. The results of this study are as follows: First, it is necessary to select or combine the following measures. they are the coordination or combination of the functions of the export support institutions, the operation of the single export support institutions, the utilization of the cooperative support system between the support institutions, the use of the private enterprises. First, it is necessary to review the following measures: they are the functional adjustment and integration among export support agencies, the adjustment of support organizations by export stage, the role coordinating between the Small and Medium Business Administration and the Local Government. Secondly, it is necessary to build a customized support system for enterprises. Thirdly, in order to secure the manpower and expertise of the support organization, it is necessary to review the utilization of the retired manpower the from the trade companies or the youth intern system. Fourthly, it is suggested that the balanced performance index is required for the export support programs with a certain scale and need to increase the portion the external evaluation together with the quantitative and qualitative evaluation.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.196-206
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• 2018

Dramatic increase in global population accompanied by rapid industrialization in developing countries has led to serious environmental, food, energy, and health problems. The Food and Agriculture Organization of the United Nations has estimated world population will increase to 9.7 billion by 2050 and require approximately 1.7 times more food, and more than 3.5 times energy than that of today. Particularly, sweetpotato is easy to cultivate in unfavorable conditions such as heat, drought, high salt, and marginal lands. In this respect, sweetpotato is an industrially valuable starch crop. To replace crops associated with these food and energy problems, it is necessary to develop new crops with improved nutrients and productivity, that can be grown on marginal lands, including desertification areas using plant biotechnology. For this purpose, exploring useful genes and developing genetically modified crops are essential strategies. Currently, sweetpotato [Ipomoea batatas (L.) Lam.] have been re-evaluated as the best health food and industrial crop that produces starch and low molecular weight antioxidants, such as vitamin A, vitamin E, anthocyanins and carotenoids. This review will focus on the current status of research on sweetpotato biotechnology on omics including genome sequencing, transcriptome, proteomics and molecular breeding. In addition, prospects on molecular breeding of sweetpotato on marginal lands for sustainable development were described.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.124-131
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• 2005

The regulation of gene expression plays an important rolet in cell cycle controls. In this study, a novel $mas3^+$ (mitosis associated protein) gene, a homolog of human SMARCADl, was isolated and characterized from a fission yeast Schizosaccharomyces pombe. The overall homology between the helicase proteins of the two species is $87\%$. This DEAD/H box-containing molecule has seven highly conserved sequence regions that allow us to place it in the SNF2 family of the helicase superfamily. Knock-out cell of $mas3^+$ gene was constructed using kanMX6 as a selection marker. Survival of mas3 null mutant exposed to UV or MMS was similar to those of wild type cells. $mas3^+$ expression was lowest at $G_2$ and gradually increased. Cytokinesis of mas3 null mutant was abnormal at $26^{\circ}C\;and\;35^{\circ}C$ and a large number of multi-septate cells were produced. These results indicate that the $mas3^+$ is involved in cytokinesis and cell shape control.

• Journal of the Korean Society for Library and Information Science
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• v.44 no.4
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• pp.249-276
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• 2010

With the rapid growth and development of information technology and the Internet, the amount of information published in electronic formats such as video, audio, digitalized text, etc. and the number of users accessing information online to satisfy their information needs are growing at a tremendous rate. This study analyzes standardized components to construct ERMS and proposes a model of ERMS based on the result of the analysis. The main functions of ERMS in university libraries are: 1) ERMS can manage and control access information to various electronic resources, metadata, holdings, user resources. Also, ERMS can be compatible with an existing library system such as IR(Information Retrieval) system, linking system, or proxy system. 2) ERMS should completely be compatible with acquisition and cataloging systems for effective management and control of integrated information organization and library budget. 3) ERMS should systematically and effectively manage license information on electronic resources. 4) ERMS should provide ideal and effective environment for use and access control of electronic resources in a library and integrated tool to manage and control all of electronic resources. Additionally, this study points out the need to organize committee groups to establish standardized rules and collaborative management of electronic resources among university libraries like DLF ERMI and redesign organizations in a library and a librarian's job description.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.80-89
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• 1996

The expression and secretion of human lactoferrin (hLf) in Sacclnromyces diastaticus were performed. 1. For the secretion of hLf in yeast, recombinant plasmid pYEGLf was constructed using promoter, secretion signal sequence of glucoamylase I gene (STA1) and transcriptional terminator of GAL7 gene. 2. Each correct recombinant plasmid was selected by mini-preparation of plasmid DNA from E coli transformant and restriction enzyme digestion analysis. The selected plasmids, pYEGLf, were transformed into S. diastaticus YIY345 as a expression host, respectively. 3. Western blot analysis using rabbit anti-hLf was carried out to identify expressed hLf. Positive signals were shown in culture supernatant of pYEGLf transformant. 4. About $100{\mu}g-1mg$ of concentrated culture supernatant of positive clone were loaded on paper disc and tested for the antimicrobial activity against E coli. However, no activity was observed. We concluded that this fact results from low concentration of hLf secreted from yeast, compared with the fact that MIC of hLf is as high as $3mg/m{\ell}$. Therefore, the purification of secreted hLf may be require to investigate the antimicrobial activity. From this study, the feasibility of low-cost production of sufficient quantities of human lactofferin for nutritional and therapeutical applications were suggested.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.3
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• pp.299-306
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• 2009

In nitrogen-limited conditions, rhizobia lead to formation of nitrogen-fixing nodules on the roots of leguminous plants. The process of nodulation is autoregulated by pre-existing nodules in the same root system. The altered profile of sap proteins by inoculation with B. japonicum may indicate presence of a signal responsible for autoregulation transferred through stem. The 20 kDa protein enhanced by innoculation significantly decreased in intensity from 2.5 to 7 days after inoculation (DAI). However 6 kDa protein did increase during such a transition period. Western blot analysis showed that both 20 kDa and 6 kDa were cross-reacted with the SKTI antiserum. This suggests that SKTI may be involved in soybean nodulation by specific induction and degradation in stem sap during early stage of nodulation. RNAi technique and Agrobacterium rhizogenes-mediated transformation were applied to investigate the function of SKTI in nodulation. We have found that the number of rhizobium-induced nodule was much less in SKTIi-silenced hairy roots than the non-silenced. Indeed the quantitative RT-PCR showed that the expression level of SKTI gene was reduced over 40% in the transgenic hairy roots compared to the non-transgenic. It appears that the observed early induction of SKTI and degradation into small peptide in a specific time manner may be involved in autoregulation of nodulation in soybean and the specific mechanism of such regulation remains to be investigated.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.412-425
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• 2012

Concrete Soil Erosion Control Dam, which blocks flow of debris flow in torrential stream, are reported to lose expected functions due to structural failure and collapses, caused by poor construction, material deterioration and external impacts. In this paper, an integrity assessment technique for debris barriers was proposed, which allows preliminary detection of problems inherent in debris barriers. The proposed integrity assessment technique is a non-destructive method based on SASW method, one of surface-wave tests. In this paper, a practical procedure and analysis guidelines in applying the SASW technique to debris barrier was proposed and its validity was verified using five decrepit debris barriers older than 20-year old. As a result, the SASW method was validated for the reliable grade evaluation method for concrete soil erosion control dam, and the resulting grades turned out to agree with the results determined by Sabang Associations.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.4
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• pp.35-48
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• 2018

This study aims to assess the training effect of QFD(Quality Functional Deployment) program for 3 years over startups, adopted from the conventional QFD widely used in the large companies to break up to a sluggish sales and growth, for employees working in startup whether the participants in startup and venture company taking this lessons into their real tasks or not. In particular, the focus of this study falls on figuring out whether individual characteristics of the participants play a role in moderating effect over transfer intension factors and its link path structure. The research results drive out two significant findings. First, in terms of relationship between the influence of transfer intension by self-efficacy and the validity of training content with the learner's readiness, the moderating effect of demographic features of the participants is effective partially by the sex and fully by their working position, but not statistically significant by age, education, and the prior startup career. This research deliver the following significant implication that the active participation of CEO level, decision-maker guarantee the higher performance of the training program like QFD program, more stresses falling on practical implementation in real business rather than just ending up with career training. This study gives significant policy implication to quasi-government organization running all public startup training projects.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.104-104
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• 2011

2010년 기준 세계 물산업은 약 4,800억불 규모이며, 2025년에는 약 1조 달러 규모로 성장할 것으로 전망된다. 또한 기후변화 등의 요인으로 물산업의 범위는 물순환체계 전과정을 포괄하는 "유역종합개발+상하수도+대체수자원"으로 확장될 것으로 예측된다. 그러나 지금까지의 국내 물산업 육성은 주로 상하수도 분야 중심으로 국한되어, 기후변화에 대응한 유역종합개발 분야에 대한 시장 진출기회를 상실하고 있다. 상하수도 중심의 물산업은 이미 선진 메이저 기업들이 선점하여 치열한 경쟁이 벌어지고 있는 '레드오션'이라 할 수 있으므로, 새로운 물산업의 강국으로 부상하기 위해서는 우리가 가진 장점을 바탕으로 물산업의 새로운 영역을 개척하는 방안에 대한 연구가 필요한 시점이다. 본 연구는 먼저 국내외 물순환체계 전과정(유역종합개발+상하수도+대체수자원)에 대한 시장 조사을 통해 세계 물산업 시장을 프로젝트 유형별, 지역별로 분석하고, 이를 토대로 국내 물산업 육성과 해외진출을 위한 당면 과제를 다음과 같이 제시하였다. 첫째, 민관협력을 위한 제도적 틀을 형성할 필요가 있다. 정부의 역할이 매우 중요한데, 정부 또는 기금이 자금의 단순한 대부자에서 적극적인 투자자로 전환함으로서 국내 민간기업들의 해외시장 진입장벽을 낮추어 줄 필요가 있다. 정부 주도의 민관협력이 활성화되면 참여 기업의 재무적 리스크를 현저히 줄일 수 있다. 또한, 상하수도 운영 경험을 축적한 공기업이 해외진출 지원기능을 수행하도록 하여야 한다. 즉, 공기업이 민간 기업의 경쟁자가 아니라 지원자가 될 수 있도록 프레임을 바꿔주어야 한다. 둘째, 물산업 클러스터의 형성이다. 물산업 제조업은 대부분 중소 벤처기업으로 독자적인 해외진출이 곤란하므로, 물전문 공기업이 중소 벤처기업 육성 및 해외진출의 앵커 역할을 담당하는 것이 필요하다. 이스라엘이나 싱가포르의 물산업 클러스터처럼 Anchor 역할을 행하는 공기업과 민간기업이 장기적 협력관계를 구축할 수 있는 기반을 마련해야한다. 셋째, 신시장 역량의 창출이다. 기후변화로 크게 성장할 전망인 통합물관리 시장에 대한 전략적 접근이 요구된다. 우선 ODA 등 대외 원조자금을 활용하여 투자비가 적게 들고 정보를 선점할 수 있는 조사 설계부터 시작하여, 댐 및 수력개발, 상하수도 건설 운영 등에 단계적으로 접근할 수 있을 것이다. 또한, 향후 도입될 예정인 물인프라의 Smart 기술, 첨단 수처리 기술 등을 활용하여 새로운 시장을 개척해야 한다. 4대강살리기 사업, 해수담수화 등 조기에 경쟁우위를 갖출 수 있는 사업과 기술을 Flagship Project로 브랜드화하여 우리나라를 "물강국"으로 포지셔닝할 경우 세계 물시장 공략에 보다 효과적일 것으로 판단된다.