• Korean Journal of Microbiology
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• v.48 no.4
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• pp.262-269
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• 2012

Seasonal differences of the cultivable bacterial communities associated with the marine sponge, Hymeniacidon sinapium, between spring and summer were analyzed through the Amplified Ribosomal DNA Restriction Analysis (ARDRA). For the cultivation of the bacterial isolates, modified Zobell and MA media were used. The 16S rDNA of individual strains were amplified and fragmented by using two restriction enzymes, HaeIII and MspI. As a result, 23 ARDRA types from the spring sponge and 28 types from the summer sponge were obtained. The partial sequencing result of 1 to 3 selected strains from each types showed over 94% similarities with the known species from the public database. The bacterial communities from the sponge, captured on spring, contained 4 phyla: Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, and Firmicutes. There were 5 phyla observed from the bacterial communities associated with the sponge, captured on summer: Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Bacteroidetes. Gammaproteobacteria was predominant group in both spring and summer, accounted for 33.8% of total in spring and 67.4% in summer, showed increase pattern on summer. Because Firmicutes and Actinobacteria participated in 30.2% and 8.3% of the spring sponge while they represented only 6.9% and 0% of the summer sponge, both bacterial groups showed decrease drift on summer. Betaproteobacteria (4.7%) and Bacteroidetes (4.7%) were only observed on the sponge captured on summer. On the sponge, Hymeniacidon sinapium, more diverse bacterial communities were shown on summer than on spring, and even from the same sponge, there were seasonal differences.

• Journal of Applied Biological Chemistry
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• v.63 no.4
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• pp.387-392
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• 2020

Pseudomonas tolaasii is a pathogen causing brown blotch disease in cultivated mushrooms. In previous study, various strains of P. tolaasii were isolated from the mushrooms with disease symptoms and they were further divided into Ptα, Ptβ, and Ptγ subtypes according to the 16S rRNA gene analysis. To investigate the secretion of peptide toxins, tolaasin and its analog peptides, culture extracts of Pt group strains were analyzed by gel permeation chromatography. Those of Ptα subtype strains contained two chromatographic peaks, band A and B. Meanwhile, those of Ptβ and Ptγ subtype strains contained mainly band A component and a little of band B. Molecular weights of toxic peptides of culture extracts were measured by MALDI-TOF mass spectrometry. In Ptα subtype strains, the peptide compositions of band A and B were same including tolaasin I (1,987 Da), tolaasin II (1,943 Da), and its two analog peptides, 1,973 Da and 2,005 Da. The strains of Ptβ and Ptγ subtype secreted many components of MW 1,100-1,200 Da, but they did not synthesize any tolaasin-like peptides. These results suggest that the only Ptα subtype strains secrete tolaasin and its analog peptide toxins and the strains of Ptβ and Ptγ subtypes have different pathogenic characters causing brown blotch disease.

• Korean Journal of Poultry Science
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• v.40 no.1
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• pp.11-16
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• 2013

Salmonella is closely related with human health of modern society which has concern increased in livestock goods consumption as well as give economic damage throughout the chicken industry such as farm, hatchery, slaughter house and processing plant. From 2007 to 2011, this study investigated Salmonella prevalence from 200 old hen delivery trucks which deliver old egg-laying hens and broiler breeders in Korea. The prevalence of Salmonella species was 38.0% in old hen delivery truck. Serogroup C1 was the most frequently detected serogroup of Salmonella, followed by the serogroups D1, C2 and B. A total of 25 serotypes were identified and Salmonella infantis was the most frequently isolated serotype. In addition, we applied disinfectant to old hen delivery truck for the reduction of Salmonella contamination. The disinfectant consists of formaldehyde, glutaladehyde and quaternary ammonium compound was applied to the trucks. Salmonella isolation rate was significantly decreased after disinfection from 38.0% to 7.5%. Disinfectant could not effectively reduce Salmonella contamination at a dilution of 1:200 which is recommended by manufacturer, but Salmonella isolation rate was significantly decreased at a dilution of 1:50. Since old hen delivery truck could be a potential vector to carry Salmonella into farms and abattoirs, chicken delivery truck should be disinfected thoroughly and sufficiently to control contamination of farms and abattoirs.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.70-73
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• 2002

본 논문체서는 2001.12월 중국국무원 의결 36호 전신체제 개혁방안에 의해 중국전신 남ㆍ북 분할계획이 확정된 이후 신산부의 분리작업을 거쳐 2002.5.16일 중국네트웍통신그룹(중국망통)의 창립식을 갖고 중국 전신에서 정식으로 분리 출범하였다 따라서 중국의 신규 중국망통은 북경, 천진, 요녕, 길림 등 북부지역 10개성ㆍ시의 통신자산 일체를 중국전신에서 이관 받아 관장하게 되어 두 회사는 상대방 관할 지역에서는 상호 영업할 수 있게 되었다. 그리고 유선전화 부문에서도 본격적인 경쟁체제가 이루어지고 있으며, 중국망통 설립으로 매출액기준 통신사업자별 시장점유율은 중국전신: 33.8% 중국네트웍통신: 17.2%; 중국이동통신: 36.6%; 중국연통: 11.3%; 중국위성과 중국철도통신: 1.1%를 차지하게 되었다. 중국전신의 분할, 중국 마이크로소프트 회사와 MOU 체결, 차이나텔레콤과 SKT간 합작회사 설립 MOU 체결, 중국 국가 정보화 영도팀 제2차 회의결과 내용, 중국의 VDSL 시장출현 둥 중국의 IT 산업정보에 대한 전반적인 내용을 다룸으로써 우리나라의 IT전망을 조망하여 본다.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.05a
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• pp.479-482
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• 2003

비휘발성 메모리인 플래시 메모리는 가볍고, 전력 소비가 적으며, 충격에 대한 저항이 강한 특징이 있다. 그러나 플래시 메모리는 세그먼트당 삭제 횟수가 최악의 경우 10만 번 정도로 제한된다는 단점이 있어서, 만약 삭제가 특정 세그먼트에 집중된다면 전체 수명이 단축되게 된다. 하지만, 플래시 메모리의 모든 세그먼트가 골고루 사용된다면 수명을 연장시킬 수 있다. 이를 위해 저장되는 자료의 유형을 파악하여 Hot, Cold 그리고 Lukewarm 이라는 그룹별로 분리해서 관리한다. 단순한 분리는 사용 횟수의 양극화를 가져오지만, 양극화된 세그먼트에 반대 유형의 자료를 할당하고 관리하면 전체 세그먼트의 사용률을 균등하게 할 수 있다. 이를 위해, 쓰기와 클리닝 작업 외에 양극화의 패턴을 통해 유형을 결정해주는 루틴을 포함하였다.

• Research in Plant Disease
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• v.21 no.4
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• pp.346-350
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• 2015

To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from 'Hongro' apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.

• Applied Chemistry for Engineering
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• v.7 no.1
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• pp.129-135
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• 1996

The entrapping capacity of the single hydrocarbons and the entrapping equilibrium data for binary mixtures of the $C_6$ to $C_9$ hydrocarbons on the activated thiourea have been investigated. The entrapping capacity of single component varied irregularly with molecular size and was independent of temperature. In the liquid phase entrapping from binary system, the lower molecular weight hydrocarbon was entrappe preferentially. In the liquid phase entrapping from trimethylbenzene isomer and ethyltoluene isomer, selectivity was found to be related to the relative position of methyl groups in the molecules and hence the electronic configuration. Pseudocumene of a purity of 99.5wt% may be obtained from $C_9$ aromatic raffinate found in naphtha cracking center. Activated thiourea was more efficient than distillation, extractive crystallization and adductive crystallization in terms of separation factor.

• Membrane Journal
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• v.20 no.4
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• pp.267-277
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• 2010

Gas separation membranes have been developed for decades in various areas to replace the conventional processes. Membrane processes for gas separation have many advantages of energy saving, compact size, and easy scale-up. Nowadays, gas separation processes is widely spreaded in nitrogen generating system, hydrogen generating system, membrane dryer, on board inert gas generating system, natural gas purification, biogas purification and fuel cells. Carbon dioxide separation process using membrane would be a strong candidate of carbon dioxide capturing process. In order to broaden the scope of application of gas separation membranes, development of new materials which can overcome the borderline of Robeson's plot should be necessary, so that many researchers and companies are trying to develop the new materials like polymers containing cardo and spiro group and PIMs (polymers for intrinsic microporosity).

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.107-112
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• 2002

To isolate the bacteria lysing cyanobacteria, the sediment samples were collected from Dochang and Pal'tang Reservoir and Seokchon Lake. Each sample was smeared on the Anabaena cylindrica lawn and incubated in light chamber for 11 days. Bacteria having cyanobacteria-Iysing activity were isolated from the samples of Seokchon reservoir. Confirmation of cyanobacteria-Iysing activity was carried out to measure chlorophyll a and bacterial cell counting in mixed culture of Anabaena cylindrica and bacteria. Lysis was detected when extracellular meterials was added to the Anabaena cylindrica culture. The isolate was identified by analysis based on 16S rDNA sequence and morphological and physiological properties. The bacterial strain was taxonomically studied by the phylogenetic analysis based on 165 rDNA sequence. This strain was identified as a member of the genus Bacillus and designated as Bacillus sp. CHS1.

• Electronics and Telecommunications Trends
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• v.8 no.2
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• pp.60-68
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• 1993

지금까지 뇌에는 컴퓨터와 같은 클럭(clock) 신호가 없고 신경들 간의 활동은 비동기적이라고 알려져 왔다. 그러나, 1989년 Singer 연구 그룹에서 시각 피질에 있는 많은 신경들이 발진하며 이들 간에는 시각 영상에 따라 동기현상을 보인다고 발표한 후, 많은 실험과 모델링이 행해지고 있다. 이 분야의 응용으로는 여러 분야가 있으며, 특히 동기 발진 모델을 이용하였을 경우에 패턴 분리와 인식에 응용될 수 있다. 본 고에서는 신경회로망의 새로운 기류인 생물학적 발진 신경망의 실험과 모델링에 대한 동향을 분석하고자 한다.