• Journal of Mushroom
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• v.11 no.4
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• pp.287-291
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• 2013

This study was carried out to improve standardization of agricultural products and investigate quality changes during storage at different treatments. The standardization does much to improve merchantable quality, distribution efficiency and fair dealings by shipping of the standard agricultural products. Therefore, modification of these standards is required to fit farmhouse situations. Average pileus diameter and thickness of A grades was 38.9 mm and 4.5 mm at shelf culture and 22.2 mm and 3.9 mm at bottle culture. Average stipe length of A grades was 49.6 mm at shelf culture and 66.7 mm at bottle culture, and stipes thick was the highest in A grades of shelf culture. The diameter of pileus of Pleurotus ostreatus stored for 14 days at $4^{\circ}C$ and $10^{\circ}C$ showed no significant difference. but the thickness of pileus decreased rapidly after 7 days. L-value and hardness of stipes and pileus were higher at shelf culture than bottle culture.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.434-442
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• 2015

The process of cultivation and production of oak mushroom (Lentinula edodes (Berk.) Pegler) on sawdust surface beds were investigated. Sawdust surface bed cultivation is the method by which oak mushrooms are cultured and produced on sterilized sawdust surface bed without using bags. The bed was made by inoculating with 3 to 1 ratio of bed sawdust to oak mushroom mycelial inoculum. The sawdust bed medium with 65% water content was pasteurized at $65^{\circ}C$, inoculated with sawdust spawn and spread on the surface on vinyl film in cultivation shed. During 78 days of cultivation period, water content in the medium varied from 61 to 72%, its pH decreased from 5.6 to 3.9~4.6 and ergosterol concentration increased to $0.33{\sim}0.59{\mu}g/g$. $CO_2$ concentration in the medium rapidly increased to 8.06% in two weeks. In seven weeks the medium surface started browning and $CO_2$ concentration increased to about 5.63%. Until 11th week the $CO_2$ concentration was maintained at 6~7%. After removing the plastic cover on the bed for ventilation in 12 weeks, $CO_2$ within the bed reduced dramatically to 1.5%. In the cultivation shed the internal temperature was $7.1{\sim}29^{\circ}C$ and humidity was 27.3 to 100%, while bed temperature ranged $11.6{\sim}30^{\circ}C$. Oak mushroom fruiting started from late July, in 120 days after bed establishment in late March and continued for approximately 100 days until early December with eight cycles of irrigation treatment. The mushroom yield of the eight cycles were 288~352 kg during the 1st (7/29~8/4) to 3rd cycle (9/3~9/7), 800 kg at the 4th cycle (9/19~9/24), 1,296~1,853 kg during 5th (10/3~10/8) to 7th cycle (4.11~11/9) and 990 kg at 8th cycle (11/23~12/7). Total production was approximately 7.4 tons from 33.0 tons of oak sawdust medium, thus harvest efficiency of the mushroom production was approximately 22.4%.

• Journal of Animal Science and Technology
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• v.52 no.2
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• pp.117-124
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• 2010

An in situ ruminal disappearance trial and an in vivo sheep metabolism trial were conducted to evaluate the nutritional value of spent mushroom substrate (SMS, originated from Pleurotus osteratus bed-type cultivation). The raw SMS was ensiled (ESMS) for 30 days with 5% (w/w, DM basis) molasses, 0.5% (v/w) yeast (Saccharomyces cerevisiae) and 0.5% (v/w) lactic acid bacteria (Lactobacillus plantarum). Two ruminally cannulated Holsteins (average BW 620 kg) were used to evaluate in situ disappearance. Six sheeps (average BW 48 kg) were fed, in $3{\times}3$ Latin square design, rice straw alone (Control), 25% (ESMS-25) and 50% (ESMS-50) of rice straw were replaced with ensiled SMS. For an in situ trial, ruminal DM and neutral detergent fiber (NDF) disappearance of SMS were increased after ensiling (P<0.05). For a sheep trial, protein and fiber (NDF, acid detergent fiber, crude fiber) digestibilities were decreased (P<0.05), crude ash digestibility was increased (P<0.001), and nitrogen retention was not affected (P>0.05) as rice straw was replaced with ensiled SMS. Ruminating time was decreased by an average of 28% by feeding ensiled SMS (P<0.05). Ensiled SMS (Bed-type cultivation) had 76% of energy value of rice straw. Consequently, ensiled SMS (Bed-type cultivation, 100% cotton waste) could be used as a roughage source appropriate for maintenance type rations for ruminants.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.79-88
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• 2007

This study was conducted to determine the minimal yield of spent mushroom substrates (SMS) and physicochemical characteristics of Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes according to mushroom species and cultivation types. The annual yield of SMS in 2004 was minimally 1,670,182M/T and 10.7-fold of the mushroom yield. The yield of SMS for Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes was 972,141M/T and was 58.2% of the total yield of SMS. Data from the chemical analysis of totally 109 SMS samples revealed that bed type cultivation showed low NDF (65.2%), high NFC (12.7%) and high ash (11.5%) contents (P<0.05), resulting in better nutritional characteristics compared with bottle or vinyl bag cultivation. In general, it was more desirable to classify SMS by cultivation types rather than by mushroom species for the effective use of SMS as an animal feed. Among cultivation types, SMS from bed type cultivation needed to be preferably used as feed.

• The Hankook-Saengyark Bo
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• no.264
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• pp.5-5
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• 2005

섬오갈피' 대량생산 길텄다.(산림과학원, 자생지서 개체 선발 생물반응기 배양) - 천마, 실내 균상재배땐 수확 '4배' - 대추 50$^{\circ}C$로 건조 상품성 가장 좋아(농촌지도기관 주문) - 표고 건조때 솔잎넣어 부패$\cdot$벌레발생 방지

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.128-133
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• 2015

Mushrooms can grow in nature when adequate temperature and humidity are maintained, but such condition can be satisfied for only a short period of time on an annual continuum. Therefore, it can be deemed that a majority of the distributed mushrooms in the current market are produced in an artificially manipulated environment. This study was conducted to resolve the problem of the Oyster mushroom cultivation house's internal temperature and humidity imbalance, where the Oyster mushrooms are cultivated in a multi-layered shelves. The air circulation fans were installed to improve the air uniformity of the Oyster mushroom cultivation house by using multi-layered shelves. During the experiment, the ambient temperature and the ambient relative humidity ranged from $5.2^{\circ}C$ to $20.4^{\circ}C$ and 40% to 100% respectively. Due to the change of the outdoor temperature, the internal temperature of the Oyster mushroom cultivation house also changed, ranging from $13.3^{\circ}C$ to $18.4^{\circ}C$, but the temperature gap between the different internal location of the facility during the conforming recorded time only ranged from $0.2^{\circ}C$ to $1.3^{\circ}C$, being significantly stable. The internal relative humidity, ranging from 82% to 96%, also changed due to the change of the outdoor temperature. Nevertheless, the relative humidity gap between the different internal location during the conforming recorded time only ranged from 2% to 7%. Furthermore, the research staff were able to maintain the concentration of $CO_2$ from 575ppm to 731ppm(below 1,000ppm was the goal) indicating the possibility of an even management of the internal environment by installing the air circulation fan.

• The Korean Journal of Mycology
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• v.27 no.2 s.89
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• pp.107-111
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• 1999

Vinyl mulching was used on Oyster mushroom beds in an attempt to control bacterial diseases of Pleurotus ostreatus. Beds were covered with perforated, transparent vinyl sheets (0.03 mm thick, 180 cm wide); holes (10 cm diameter) were 10 cm apart. There were $25\;holes/m^2$. Vinyl mulching made management of the crop easier. Discrete mushroom flushes formed with high quality bunches of mushrooms formed through the holes; this made picking quicker and made more efficient use of labor. Total yield of mushrooms increased by 5.7% to $10.8\;kg/m^2$. Mushroom mycelium under vinyl remained healthy and white; whereas mycelium grown using conventional methods changed from white to yellow-brown during the cropping cycle. The mean weight of a mushroom bunch from vinyl mulched beds was 283 g (33 fruitbodies) compared to 117 g (15 fruitbodies) obtained using conventional growing methods.

• The Korean Journal of Mycology
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• v.15 no.2
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• pp.92-98
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• 1987

Twelve species of fungi were isolated from rice straw media for oyster mushroom cultivation. Trichoderma, Aspergillus and Rhizopus were the predominant fungi. Seven species of Trichoderma were isolated and identified from the rice straw media and the order of their frequency in the media was pseudokonigii, aureoviride, viride, harzianum and koningii. Occurrence of harmful fungi in mushroom houses become more severe as the number of cultivation times increased, and that was more severe in spring culture than in autumn culture. Mycelial growth and sporulation of Trichoderma, Aspergillus and Rhizopus were fovorable on the media appended with extracts of rice straws and oyster mushrooms. This results indicate that the rice straw media and mushrooms give favorable conditions for the occurrence of the fungi in the mushroom houses. Mycelial growth of Trichoderma spp. was favorable on saw­dust extraction media and rice bran extraction media, and the spawns inoculated at the mushroom beds present media of the fungi.

• Korean journal of applied entomology
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• v.35 no.1
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• pp.45-51
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• 1996

As the pest of the Letinus edoeds, five insect species and six animal species are investigated injuring the fresh mushroom. Of these, insect and animals, Achorutes armatus, Scaphidium amurense and Philomycus confusa caused severe damage to mushroom fruitbody, and a greate deal of damage was done to mushroom lamella by mites (Rhizoglyphis sp., and Histiostoma sp.). As the pest of Lentinus edodes bed logs, two insect species from immature bed logs, eight insects and one isopod species from matured bed logs were investigated. Of those, Moechotypa diphysis was found to be the most injurious species from both immature and matured bed logs. As the pest of Pleurotus ostreatus, six insects and the two mite species were investigated from sporophore. Of those, Lycoriella sp. and Mycophilla sp. are considered to be very serious. while two insects and five mite species were investigated from the mushroom bed. Of those, one insect (Lycoriella sp.) and two mites (Histiostoma feroniarum and Throglyphus bankslogior) found to be abundant and caused serious damage.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.52-56
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• 2000

느타리 버섯의 다양한 품종과 재배형태가 급변히 요구되는 시기에 충남농업기술원 버섯연구팀의 5년 (1996～2,000)에 걸쳐 국,내외 수집 20 균주를 공시하여 개발 육성한 청풍과 명월 신품종 느타리버섯을 현재 재배되고 있는 기존 느타리품종과 동일여부를 위해 PCR-RAPD분석과 배양적, 재배적 특성을 인공재배 시험한 결과를 요약하면 다음과 같다. 1. 청풍, 명월느타리버섯 균사배 양 온도는 청 풍은 20～$25^{\circ}C$,명 월은 25～3$0^{\circ}C$, 이고 배지는 PDA배지가 양호하고 균사생육은 청풍, 명월 모두 비슷하였으며 배지 pH는 6.5～7.5 였다. 2. 버섯재배가능은 청풍은, 5～26$^{\circ}C$ 명 월은, 7～28$^{\circ}C$로 온도범위가 넓어 재배가 용이하나 적정 생육온도는 청풍, 명월 공히 15～19$^{\circ}C$가 양호 하였다 3. 청풍과 명월의 배양온도 23$^{\circ}C$에서 균사배양기간 23～24일 (850cc/pp),폐면 균상 재배에서 균배양 기간은 청풍, 명월 공히 11～12일, 초발이 소요일수는 2～3일 소요 되었다. 4.초발이시 청풍과 명월 갓 색택은 진청색이고, 생육후기 청풍은 회갈색, 명월은 진회색으로 조직이 치밀하고 저장성이 양호하여 경제성이 기대된다. 5. 다발수는 춘추2호 (13개/다발) 대 비 청 풍11개, 명월12개로 약간 적었으나 개체중은 춘추2호(14g/개) 대비 청풍 16g/개, 명월 14g/개로 비슷한 경향 이었다. 6. 수량성은 균상 폐면재배시 춘추2호 (40kg/3.3$m^2$)대비 청풍 느타리버섯은 (46kg/3.3$m^2$) 15%증수되고, 명월느타리버섯은(41kg/3.3$m^2$)로 3% 증수되었으며 병재배 겸용도 가능하였다. 7. PDA대치배양과 PCR-RAPD시험에서 청풍, 명월 신품종느타리 버섯은 기존 재배 되고 있는 느타리품종과 다른 품종으로 확인 되었다.