• Title/Summary/Keyword: 귀리

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Isolation of Nitrogen-Fixing Bacteria from Gramineous Crops and Measurement of Nitrogenase Activity (벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정)

  • 최은화;이상은;윤기순;권덕기;손재근;박승환;한명숙;김사열
    • Microbiology and Biotechnology Letters
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    • v.31 no.1
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    • pp.18-24
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    • 2003
  • For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.

Cytotoxic Effect of Variable Crops (수종의 작물에 대한 세포독성효과)

  • 문형인
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.346-350
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    • 1997
  • Antineoplastic activities against human gastric and colon carcinoma cell lines were measured in seventeen methanol extracts of seventeen food and medicinal crops using the MTT(3-[4.5-dimethyl thiazol-2-yl)-2.5-diphenyl tetrazolium bromide) method. Three extracts(Hodeum vulgare, Schizandra chinensis and Zea mays)from seventeen tested crops have been reported to have antineoplastic effects. The extracts obtained from remaining fourteen tested crops failed to show significant cytotoxic effect at the concentration of less than $230{\mu}g/ml$(50% inhibitory concentration).

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Effects of Butachlor on Cell Division and Cell Enlargement in Oat (Avena sativa L.) (Utachlor가 귀리 (Avena sativa L.)의 세포분열 및 신장에 미치는 영향)

  • 김재철
    • Journal of Plant Biology
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    • v.29 no.3
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    • pp.167-173
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    • 1986
  • The effects of varying concentrations and durations of butachlor [N-(bytoxymethyl)-2-chlor-2', 6';-diethylacetanilide] treatment on oat (Avena sativa L.) root cell division were studied. Oats were treated from 0 to 48h with concentration ranging from 1$\times$10-6M to 1$\times$10-3M of butachlor. The highest concentration (1$\times$10-3M) of butachlor caused significant inhibition of cell division after 6h treatment. After 18h treatment, 49% and 66% inhibition of cell division occurred at 1$\times$10-5M and 1$\times$10-4M, respectively, while 16% inhibition of cell division occurred at 1$\times$10-6M concentration at same exposure period. Oat treated with 1$\times$10-5M and 1$\times$10-6M showed 69% and 38% inhibition of cell division after 36h. Increasing herbicide concentration at a specific time increased inhibition of cell division, and increasing the duration of treatment at a specific concentration also increased inhibition of cell division. In most instances the greatest inhibition of cell division occurred between 0 to 18h during 48h treatment. A range of concentration of 1$\times$10-5M to 1$\times$10-3M reduced cell enlargement significantly during 24h incubation period. The 1$\times$10-5M and 1$\times$10-3M caused 34% and 75% inhibition of cell enlargement. It was concluded that butachlor caused the growth inhibition of oats by inhibiting both cell division and cell enlargement.

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Antioxidant Activity of Ethyl acetate Fraction of Oat in Caenorhabditis elegans (귀리 Ethyl acetate 분획물의 예쁜 꼬마선충 내의 항산화 효과)

  • Kwon, Kang Mu;Kim, Jun Hyeong;Yang, Jae Heon;Ki, Byeolhui;Hwang, In Hyun;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.52 no.4
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    • pp.251-256
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    • 2021
  • Oat, the seeds of Avena sativa L. (Gramineae), is an important dietary staple for people in many countries. Previous studies reported that A. sativa had various pharmacological effects such as anti-inflammatory, antitumor, neurotonic, and antispasmodic activities. In this study, Caenorhabditis elegans model system was used to investigate the antioxidant activity of methanol extract of oat. The ethyl acetate soluble fraction of the oat methanol extract showed the best DPPH radical scavenging activity. The ethyl acetate fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction, SOD-3 expression was measured using GFP-expressing transgenic worm. As a result, the ethyl acetate fraction increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. In addition, the ethyl acetate fraction-treated CF1553 worm showed higher SOD-3::GFP intensity compared to the control.

Occurrence of Entomophthora muscae Isolated from Delia platura in South Korea (Delia platura로부터 분리된 Entomophthora muscae의 특성)

  • Kyung-Gu Min;Jeong-Heon Kim;Tae-Min Park;Eui-Yong Hong;Yu-Bin Park;Jin-Woo Lee;Youn-Jin Park;Myoung-Jun Jang
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.505-510
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    • 2023
  • In June 2023, at the oat field on Kongju National University's Yesan Campus, flies infected with an entomopathogenic fungus were discovered. The flies were hanging with their wings folded backward. Fungal mycelia had sprouted on the abdomens of the flies. An analysis of the 28S ribosomal RNA sequence confirmed that the fungus was Entomophthora muscae. Moreover, cytochrome oxidase sequence analysis of the host insect identified it as Delia platura.

Mode of Action of Water Soluble β-Glucan from Oat (Avena sativa) on Calorie Restriction Effect In-Vitro and In-Vivo Animal Models (In-Vitro, In-Vivo 동물모델에서 귀리 유래 수용성 베타-글루칸의 칼로리 제한 효과 작용기전 규명)

  • Kang, Hanna;Kim, Se-Chan;Kang, Yong Soo;Kwon, Young-In
    • The Korean Journal of Food And Nutrition
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    • v.30 no.6
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    • pp.1222-1228
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    • 2017
  • In the current study, we investigated the inhibitory activity of water soluble ${\beta}-glucan$ from oat (Avena sativa) against various digestive enzymes such as ${\alpha}-glucosidase$, sucrase, maltase and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can significantly decrease the post-prandial increase of blood glucose level after a mixed carbohydrate diet. The ${\beta}-glucan$ had the highest documented rate of small intestinal sucrase inhibitory activity (2.83 mg/mL, $IC_{50}$) relevant for potentially managing post-prandial hyperglycemia. Furthermore, we evaluated the effects of ${\beta}-glucan$ on the level of post-prandial blood glucose in animal model. The post-prandial blood glucose levels were tested two hours after sucrose/starch administration, with and without ${\beta}-glucan$ (100, and 500 mg/kg-body weight). The maximum blood glucose levels (Cmax) of ${\beta}-glucan$ administration group were decreased by about 23% (from $219.06{\pm}27.82$ to $190.44{\pm}13.18$, p<0.05) and 10% (from $182.44{\pm}13.77$ to $165.64{\pm}10.59$, p<0.01) in starch and sucrose loading test, respectively, when compared to control in pharmacodynamics study. The ${\beta}-Glucan$ administration significantly lowered the mean, maximum, and minimum level of post-prandial blood glucose at 30 min after meal. In view of the foregoing, it is felt that our findings suggest that ${\beta}-glucan$ from oat serves to reduce post-prandial blood glucose rise secondary to slower absorption of glucose in the small intestine, via carbohydrate hydrolyzing enzymes inhibition.

Survey on the Pests of Stored Garlic (저장마늘을 가해하는 해충조사)

  • 나승용;조명래;김동순;박권우;우종규;김기택
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.65-71
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    • 1998
  • Survey was conducted on the kinds and densities of pests associated with stored garlic collected from farms of major garlic production areas from 1994 to 1995 in Korea. Aceria tulipae, Rhizoglyphus sp., Ditylenchus dipsaci, and Tyrophagus putrescentiae were frequently detected with high densities and Tarsonemus bilobatus and Aphelenchus avenue showed relatively low detection rates and densities. Detection rate of A. tulipue was 38% in 1994, but the rate was 65% in 1995. Number of the mite ranged from 1 to 4,500 per scale. Detection rate of Rhizoglyphus sp. was 63% in 1994, but the rate was 13% in 1995 and average number of the mite ranged from 1 to 135 per scale in 1994. Garlics damaged by Rhizoglyphus sp. showed decaying symptom. T~rophagusp utrescentiae was detected from 22 farms among 32 farms surveyed in 1994 and from 21 farms among 39 farms surveyed in 1995. However, number of the mite on garlic scale was relatively lower than the other mites and its damages on stored garlic was not determined.

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Quality Characteristics of Cookies Prepared with Oat and Barley Powder (귀리분말과 보리분말 혼합비율에 따른 쿠키의 품질특성)

  • Kim, Bo Young;Choi, Hee Sun;Lyu, Eun Soon
    • Korean journal of food and cookery science
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    • v.30 no.4
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    • pp.428-434
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    • 2014
  • Physicochemical properties, sensory characteristics, and consumer acceptability of cookies produced with oat and barley powder were evaluated. Oat and barley powder was incorporated into cookie dough at 6 levels: oat : barley = 100:0 (S1), 80:20 (S2), 60:40 (S3), 40:60 (S4), 20:80 (S5), 0:100 (S6) and control. pH of the cookie dough was lowest in the S6 group and highest in the control, S1, S2, and S3 groups. There were no significant differences in density. The spread ratio and loss rate of cookies increased significantly with an increase in content of barley powder. The leavening rate of the S1 cookie group was the lowest. With regard to color, the a-values and b-values of cookies increased with a rise in content of oat powder, and the L-value increased with an increase in barley powder content. Analysis of cookie texture showed that hardness was highest in the S6 group with barley and lowest in the S1 group, but there was no significant difference between the control group and other experimental groups. In the sensory evaluation of cookies, there was no significant difference between the control and experimental groups in roasted nuttiness and sweetness. In overall acceptance of cookies, S6 was the least acceptable and S1, S2, and S3 were the most acceptable. Results suggest that oat powder is a suitable ingredient for increasing the functionality of cookies and consumer acceptability.

A Study on Functional Senior Blended Soymilk with Enhanced Nutrition Using Soybeans and Oats (대두와 귀리를 첨가하여 영양을 강화시킨 기능성 시니어 혼합 두유 개발에 관한 연구)

  • Kim, Jeong-Yeon;Choi, Kwang-Jin;Kang, Chin-Yang;Choi, Won-Cheon;Choi, Il-Sook;Shin, Kyung-Ok
    • The Korean Journal of Food And Nutrition
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    • v.33 no.2
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    • pp.194-203
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    • 2020
  • The purpose of this study was to develop a functional senior mixed soymilk for seniors and to provide functional senior-mixed soymilk to prevent disease outbreaks. The isoflavone content of senior soymilk was 0.15±0.01 mg/g. The β-glucan content of senior soymilk was 9.82±0.01 mg/g. The weight gain of mice were significantly higher in the high-fat diet group than in the other groups. The serum triglyceride content was high at 102.67±40.61 mg/dL in the high-fat diet group, but significantly lower at 83.00±42.43 mg/dL in the 20% senior soymilk group. Thus, the results of this study comprehensively suggest that food intake should be ingested for each life cycle with reference to the dietary reference intakes for Koreans. In particular, the elderly in their 50s and older are deficient in protein and weakened immune capacity, so it is imperative that they maintain their health through various foods such as soymilk, which is evenly nutritious.

Recent Advances in the Use of Enzymes for Environment-Friendly Swine Diets (환경친화성양돈사료를 위한 효소제 사용의 최근 경향)

  • Hong Jong-Wok;Kim In-Ho
    • Korean Journal of Organic Agriculture
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    • v.9 no.2
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    • pp.55-67
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    • 2001
  • Exogenous enzymes which, for the purpose of this paper, include phytase, $\beta$-glucanase, pentosanase and $\alpha$-galactosidase, are now extensively used throughout the world as aditives in swine diets. The chemical effects of these enzymes are well understand. However, the manner in which their benefits to the swine are brought about is still under debate. Phytase was to increase the availability of plant phytate phosphorus, which reduces phosphorus pollution and allows reductions in the amount of inorganic phosphate used. Also, enzymes have been discovered which have the potential to break down deleterious compounds commonly found in swine rations such as $\beta$-glucanase contained in barley and oats and the soluble pentosans found in rye and wheat thus increasing the digestibility of these non-starch polysaccharides. Future research in these area will allow for more efficient use of the current enzymes, development of more efficient future products and development of more thermotolerant enzymes.

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