• Title/Summary/Keyword: 국내 학회지

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Technique to Generate Sterile Males of Striped Fruit Flies, Zeugodacus scutellata, using Electron Beam Irradiation and their Application to Genetic Control (전자빔 조사를 이용한 호박꽃과실파리의 불임 수컷 제조 기술과 유전방제 응용)

  • Kim, Yonggyun;Al Baki, Md. Abdullah;Kwon, Gimyeon
    • Korean journal of applied entomology
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    • v.59 no.1
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    • pp.25-35
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    • 2020
  • The striped fruit fly, Zeugodacus scutellata, is endemic in Korea, but it has been regarded as one of the serious quarantine pests throughout the world. Sterile insect release technique (SIT) has been used to eradicate quarantine fruit flies. This study developed a technique to generate sterile males and applied SIT to control Z. scutellata. First of all, the reproductive systems of Z. scutellata adults were examined with fluorescent microscope. Polytrophic ovaries comprises of around 100 follicles with developing oocytes. Each follicle contains an oocyte with several nurse cells and are surrounded with follicular epithelium. Oocyte development began at 10 days after adult emergence (DAE) and formed chorionated oocytes after 20 DAE. On the other hand, male testes were well developed just after adult emergence. The vas deferens was filled with motile sperms. To generate sterile males, different doses (0~1,000 Gy) doses of electron beam were irradiated to 3~5 days old pupae of Z. scutellata. When male pupae were irradiated with electron beam at 200 Gy, they developed and mated with females without any significant difference compared to untreated males. Although the untreated females mated with the 200 Gy-irradiated males laid eggs, no eggs did not hatch. The 200 Gy-irradiated males were then applied to untreated male and female flies in a density ratio of 1:9 (untreated males : treated males). The laid eggs suffered significant infertility. These results suggest that electron beam-irradiated pupae at 200 Gy resulted in male sterility and the resulting males would be applied to SIT.

The Effects of Aminophylline on the Superoxide Anion Generation of Neutrophils from Established Human Sepsis Caused by Acute Pneumonia (급성 폐렴에 의한 패혈증 환자에서 Aminophylline이 혈중 호중구의 과산화물 음이온 유리에 미치는 영향)

  • Kim, Yong-Hoon;Park, Jun-Young;Cha, Mi-Kyong;Lee, Sang-Moo;Kim, Hyeon-Tae;Uh, Soo-Taek;Chung, Yeon-Tae;Park, Choon-Sik
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.1
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    • pp.16-22
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    • 1993
  • Background: The Microbicidal and cytotoxic activities of neutrophils are to a large extent dependent on a burst of oxidative metabolism which generates superoxide anion, hydrogen peroxide, and other reactive products of oxygen. The respiratory burst of PMN is initiated by intracellular calcium mobilization that follows immune or particular stimulation and is very sensitive to modulation by c-AMP or adenosine. Despite its antagonism against adenosine, earlier study has demonstrated potent theophylline inhibition of the PMN respiratory burst at variable ranges of blood concentrations of theophylline in the healthy normal volunteers and in the septic animals pretreated or early post-treated with aminophylline (AMPH) or pentoxifylline. However it is unclear whether theophylline inhibits the superoxide generation or not in the established human sepsis caused by acute pneumonia, as taking into consideration of the fact that full activation of neutrophils have occurred within minutes after the septic insult in the animal experiments. Methods: We measured the $O_2$ generation of peripheral arterial neutrophils obtained from 11 human septic subjects caused by acute pneumonia before and 1 hour after completion of continuous AMPH infusion. Patients were identified and studied within 48 hour of admission. All subjects were administered an intravenous loading and maintenance dose of AMPH. The generation of $O_2$ was measured at a discrete time point (60 min) by the reduction of ferricytochrome c.PMA (10 ${\mu}g/ml$) was used as a stimulating agent. PMNs were isolated at a concentration of $2{\times}10^6$ cells/ml. The arterial oxygen tension, blood pressure and heart rates were also checked to evaluate the systemic effects of AMPH in the acute pneumonia. Results: The mean serum concentration of AMPH at 60 minutes was $8.8{\pm}0.6{\mu}g/ml$. Sixty minutes after AMPH infusion the generatition of $O_2$ was decreased from $0.076{\pm}0.034$ to $0.013{\pm}0.004$(OD) (p<0.05) and from $0.177{\pm}0.044$ to $0.095{\pm}0.042$(OD) (p<0.01) in the resting and stimulated PMNs respectively. $PaO_2$ was not changed after AMPH infusion. Conclusion: AMPH may compromise host defense by significant inhibition of neutrophil release of superoxide anion and it had no effect on improving $PaO_2$ in the acute pneumonia.

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Clinical Features of Patients with Lung Cancer and Upper Aerodigestive Tract Cancer (폐암과 상부소화호흡기암이 동반된 환자의 경우 임상적 특징)

  • Lee, Chang Youl;Chung, Jae Hee;Chang, Yoon Soo;Kim, Se Kyu;Kim, Hyung Jung;Chang, Joon;Kim, Sung Kyu;Ahn, Chul Min
    • Tuberculosis and Respiratory Diseases
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    • v.62 no.4
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    • pp.284-289
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    • 2007
  • Background: To define the clinical features of patients with lung and upper aerodigestive tract cancer through a review of the histopathology, clinical features and follow-up results. Methods: Patients with lung and upper aerodigestive tract cancer who were diagnosed in Young dong Severance Hospital from 1992 to 2005, were retrospectively reviewed. The clinical data, radiologic findings, pathologic findings, treatment modalities were evaluated. Result: There was a total of 20 patients with aerodigestive tract cancer who were diagnosed with lung cancer over a 13 years period. The mean age was $58.45{\pm}15.09$ years and 19 cases were male. There were 14 smokers with an average pack year of 46 years. Twelve patients had aerodigestive tract cancer and later developed lung cancer, and 5 lung cancer patients were later diagnosed with aerodigestive tract cancer. Conclusion: These results suggest that cancers of the aerodigestive tract and lung can arise as either dependent or independent events and most aerodigestive tract cancer patients who developed lung cancer are not treated properly. Therefore, regular low dose chest CT with close suspicion is needed to properly manage upper aerodigestive tract cancer patients.

Impacts of Entrepreneurship and Market Orientation of Business Incubator CEO's on business performance: Focusing on Moderating effects of utilization level of BI Supporting Services (창업보육센터 입주기업 CEO의 기업가정신 및 시장지향성이 경영성과에 미치는 영향: 창업기업의 지원서비스 활용정도 조절효과를 중심으로)

  • Kim, Seong Il;Lee, Woo Jin
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.11 no.5
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    • pp.1-13
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    • 2016
  • This study aims to observe the impacts of entrepreneurship and market orientation of Seoul's business incubator (BI) CEO's on business performance, as well as investigate the moderating effects of BI supporting services. The main objectives of this study are as follows: first, to observe how the entrepreneurship of CEO's affects business performance; second, to observe how CEO's market orientation as a cultural factor attributes to business performance; third, to verify whether the utilization level of BI supporting services shows moderating effects between entrepreneurship and business performance, and finally to verify whether the utilization level of BI supporting services shows moderating effects between market orientation and business performance. Business incubators in Seoul registered with the Business Incubator Association were surveyed both online and offline to receive 127 responses out of 320 circulations. By filtering out insincere reponses, 117 were used for statistical analysis. Our results assert that the utilization level of physical BI supporting services does indeed strengthen the relation between initiative and financial performance. Also, it is interesting to note that the utilization level of physical BI supporting services supports the relation between risk sensitivity and financial performance. While risk sensitivity on its own shows no positive effect on business performance, a suitable utilization level of BI supporting services is likely to strengthen the connection. In addition, the utilization level of both physical and non-physical supporting services shows a moderating effect between initiative and non-financial performance. Based on the results above, risk-taking business incubator CEOs with initiative are likely to yield better financial performance in terms of increasing sales, if provided with various physical supporting services such as sufficient educational facilities including communal spaces and conference rooms.

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A Survey of Japanese Perception of and Preference for Korean Foods (한국음식에 대한 일본인의 인식 및 기호도 조사)

  • ;;;;Kimiko Otan;Takahisa Minamide
    • Korean journal of food and cookery science
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    • v.14 no.2
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    • pp.188-194
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    • 1998
  • The purpose of this study was to provide those abroad with information about Korean foods and food culture by investigating the Japanese perception and preference for Korean foods. The results were as follows: 53.2% of the Japanese students had much interest in Korean foods and most of students (93.2%) were experience In eating Korean foods. On their first impression of Korean foods, 67.8% of the students answered that it was 'good', and 74.1% of answered their impression after eating Korean foods was 'good'. 83.1% of the students also answered that Korean foods suited their taste because they were 'delicious'. But 16.9% of the students answered that the Korean foods did not suit them because they were too spicey. The most well-known (99.3%) and eaten (96.3%) Korean food by Japanese stutents turned out to be Kimchi. In the preference test for Korean foods, Samgyetang was estimated the most delicious food. In the comparision between Korean Kimchi and Japanese Kimchi, the former was estimated more delicious and spicier than the latter.

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An Empirical Study on the Oriental Herbal Cosmetics Purchase Behaviors in Women in the Metropolitan Area (한방 화장품 구매행동에 관한 실증적 연구 - 수도권 거주 여성 소비자를 중심으로 -)

  • 엄정녀;김주덕
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.1
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    • pp.93-102
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    • 2004
  • Recently, the golden age of herbal cosmetics has come. Along with active introduction of oriental herbal lines, diversification of distribution channels is designated as a major feature. In this background, the present study attempts to consider the domestic market for oriental herbal cosmetics, which is growing rapidly with the introduction of various new brands, and examine the perceptions of this new type of cosmetics by women consumers based on their purchase behaviors, and search for the ways for its promotion and development. A survey was conducted to adult women consumers aged 19∼60 residing in Seoul or Gyeonggi-do. Out of a total of 430 surveys distributed, 350 answer sheets were used for the analysis Among the results, the first-hand information on the herbal cosmetics market, their usage, and the consumer needs obtained in the present study will serve as a fundamental data for planning the marketing strategies for the oriental herbal cosmetics.

Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences (ITS 염기서열을 이용한 한국산 참취속 식물의 유연관계분석)

  • Hong, Su-Young;Cho, Kwang-Soo;Yoo, Ki-Oug
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.178-184
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    • 2012
  • This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native $Aster$ species and to resolve their relationship among Korean native $Aster$, including $Kalimeris$, $Gymnaster$, $Heteropappus$ genus separated from $Aster$ in a previously study based on the pappus length. We registered 11 ITS sequences of $Aster$ species including $A.$ $glehni$ to GenBank and those sequences were used for the cluster analysis with $Kalimeris$ species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. $Kalimeris$ sensu strict substitution rate was lower than that of $Aster$ sensu strict species. The strict consensus parsimonious cluster analysis showed $A.$ $tripolium$ is the first branching from the clade and the next is $A.$ $scaber$. The $Kalimeris$ species except for the $A.$ $hispidus$ were grouped into the same clade with high bootstrap value (91%) within $Aster$. $Gymnaster$ and $Heteropappus$ that has been classified by morphological characters were also grouped into broad sense $Aster$ clade. These results implied these three genera could be merged together into $Aster$ based on the ITS sequences.

Characterization of Copper Toxicity Symptoms and Determination of Tissue Critical Concentration for Diagnostic Criteria in Korean Bred Strawberries (국내육성 주요 딸기 품종에서 발생하는 구리(Cu) 과잉 증상 및 영양진단을 위한 식물체 내 한계농도)

  • Choi, Jong Myung;Nam, Min Ho;Lee, Chiwon W.
    • Horticultural Science & Technology
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    • v.30 no.5
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    • pp.477-483
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    • 2012
  • This study was carried out to investigate the influence of copper concentrations in fertilizer solution on the growth of and nutrient uptake by domestically bred strawberries. The characterization of toxicity symptoms as well as tissue analyses based on dry weight of above ground tissue were also conducted to determine the threshold levels in plants when toxicity developed in copper. The dry weights of the above ground tissue were not significantly different among the treatments of 0.25 mM to 1.0 mM in 'Keumhyang' and 'Maehyang' strawberries and that of 0.25 mM to 3.0 mM in 'Seolhyang' strawberry. This indicates that the 'Seolhyang' strawberry is more tolerant to copper toxicity than 'Keumhyang' or 'Maehyang' strawberries. Application of copper at high concentrations resulted in severe toxicity such as death of extensive areas of leaves. The lower leaves became yellow and die rapidly as the symptoms spread up the plants. The leaf blades and petioles died back to the crown and hang on by mechanical attachment. Symptoms of copper toxicity in lower leaves developed as browning on leaf margins and in patches between leaf veins that became necrotic. The elevation of copper concentrations in fertilizer solution did not influence the tissue phosphorus, potassium, calcium, and magnesium contents based on the dry weight of the above ground tissue. The tissue copper contents increased lineally as the copper concentrations in fertilizer solution were elevated. But the tissue iron, manganese and boron contents were not influenced by the concentrations. When the concentration of copper at which growth of a plant is retarded by 10% is regarded as threshold level, the copper contents based on dry weight of above ground plant tissue should be lower than 71.4, 57.9 and 74.8 $mg{\cdot}kg^{-1}$ in 'Keumhyang', 'Maehyang' and 'Seolhyang' strawberries, respectively. The symptom characterization and established threshold level in copper toxicity would help growers to prevent the reduction of crop growth and yield in 'Seolhyang' strawberry cultivation.

Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum (국화의 GM 이벤트 선발을 위한 실질적 동등성 및 환경위해성 평가)

  • Lim, Jin Hee;Shim, Myung Syun;Oh, Kwan Hun;Seo, Ji Yeon;Lee, Su Young;Park, Sang Kun;Shin, Hak Ki;Cho, Myoung Rae
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.517-524
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    • 2014
  • As an assessment of substantial equivalence and environmental risks of branchless chrysanthemum GM lines (LeLS69, LeLS76, LeLS80) developed in Korea, we investigated their degree of branchlessness and developmental characteristics including growth and flowering, as well as the stability of populations of insect pests and soil microbes. The GM lines were compared with a control variety, 'Jinba'. LeLS80 showed the highest level of branchlessness among the three GM lines, whereas abnormal axillary buds developed in lines LeLS76 and LeLS69. There were no significant differences between the growth characteristics of 'Jinba' and the GM lines. The branchlessness LeLS80 was increased during the summer season, suggesting that it is a useful germplasm for developing elite branchless cultivars. These GM lines showed no significant differences in the occurrence of five insect pests including aphids, mites, leaf miners, thrips, and whiteflies. Similarly, for soil microbes including bacteria and actinomycetes, there were no significant differences between 'Jinba' and the GM lines. However, there was a higher occurrence of fungi in the GM lines compared to 'Jinba'. Our findings suggest that 'Jinba' and the GM lines are substantially equivalent, although further monitoring studies are needed to confirm this.

Effect of Nutrient Solution Strength on Growth, Fruit Quality and Yield of Strawberry 'Mehyang' in Hydroponics (배양액의 농도가 딸기 '매향' 생육, 과실의 품질 및 수량에 미치는 영향)

  • Jun, Ha Joon;Byun, Mi Soon;Liu, Shi Sheng;Jeon, Eui Hwan;Lee, Yong Beom
    • Horticultural Science & Technology
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    • v.31 no.2
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    • pp.173-178
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    • 2013
  • Experiments were conducted to investigate the optimum concentration of nutrient solution for strawberry 'Maehyang' bred domestically for exportation in hydroponics. Nutrient solutions for strawberry, which was made by Yamazaki, were supplied electrical conductivity (EC) 0.6, 0.8, 1.2, and $1.8dS{\cdot}m^{-1}$ after planting on cocopeat medium during the experiment period. Growth of shoot of strawberries did not show any statistical differences among treatments. Fruit length showed the longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$, followed by 0.6 and $1.8dS{\cdot}m^{-1}$ in the first and third cluster. It showed the shortest in EC $1.8dS{\cdot}m^{-1}$ in the second cluster but there were no significant differences among treatments in the fourth cluster. Fruit diameter did not show significant differences among treatments in the first and second cluster but was the longest in the lowest concentration EC $0.6dS{\cdot}m^{-1}$ in the third cluster. The shortest was in EC $1.8dS{\cdot}m^{-1}$ in the fourth cluster. The heaviest mean fruit weight appeared in EC 0.8 and $1.2dS{\cdot}m^{-1}$, and the lightest was in EC $1.8dS{\cdot}m^{-1}$ in the first cluster and also lightest in EC $1.8dS{\cdot}m^{-1}$ but no significant differences was found among other treatments in the second & third cluster. Also the fruit weight was significantly light in plants grown in EC $1.8dS{\cdot}m^{-1}$ than $0.6dS{\cdot}m^{-1}$ in the fourth cluster. Soluble solids content of fruit was the highest in EC $0.6dS{\cdot}m^{-1}$ in all cluster. As a result, we came to the conclusion that the optimum EC for strawberry 'Maehyang' was EC 0.8 - $1.2dS{\cdot}m^{-1}$ during low temperature season. This result will be utilized as an indicator for strawberry hydroponics.