• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.17 no.2
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• pp.87-94
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• 2012

To compare monthly variations of phytoplankton biomass and community composition between in seawater and sediment of the Gomso Bay (tidal flat: approximately 75%), the photosynthetic pigments were analyzed by HPLC every month in 1999 and every two months in 2000. Ambient physical and chemical parameters (temperature, salinity, nutrients, dissolved oxygen, and chemical oxygen demand) were also examined to find the environmental factors controlling structure of phytoplankton community. The temporal and spatial variations of chlorophyll a concentration in seawater were correlated well with the magnitude of freshwater discharge from land. The biomass of microphytobenthos at the surface sediments was lower than that in other regions of the world and 2-3 times lower than phytoplankton biomass integrated in the seawater column. Based on the results of HPLC pigment analysis, fucoxanthin, a marker pigment of diatoms, was the most prominent pigment and highly correlated with chlorophyll a in seawater and sediment of the Gomso Bay. These results suggest that diatoms are the predominant phytoplankton in seawater and sediment of the Gomso Bay. However, the monthly variation of chlorophyll a concentration in seawater at the subtidal zone was not a good correlation with that in sediment of the Gomso Bay. Although pelagic plankton was identified in seawater by microscopic examination, benthic algal species were not found in the seawater. These results suggest that contribution from the suspended microphytobenthos in the tidal flat to the subtidal zone of the Gomso Bay may be low as a food source to the primary consumer in the upper water column of the subtidal zone. Further study needs to elucidate the vertical and horizontal transport magnitude of the suspended microphytobenthos in the tidal flat to the subtidal zone.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.409-411
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• 2003

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the west coast and the south coast 47 area of Korea at 2001 September. Separated 13 samples of changes with red color under $28{\sim}32\;^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in Basal medium. For a pure isolation from 13 samples it used agar-shake tube method (0.4 % agar) and it separated 5 strains through 13-repetition test. The RAPD(Random Amplified Polymorphic DNA)-PCR result of strains (EGH-9, EGH-13, EGH-23, EGH-24, EGH-30) that EGH-24 and EGH-30 was same strain. For wastewater biodegradation test that 4 isolation strains cultivated in synthesis wastewater in 7 days. EGH-24 was high 63000 mg/L (CODcr) to 43400 mg/L (CODcr). EGH-24 was selected with efficient wast water treated strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 165-rDNA phylogenetic analysis, this strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

• Korean Journal of Environmental Agriculture
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• v.6 no.2
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• pp.73-76
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• 1987

The effects of light, active oxygen, inorganic $SO_3\;^{-2}\;ion$, $SO_2$ fumigation duration, $SO_2$ concentration on the degradation of photosynthetic pigments in Green perilla (Perilla frutescens Suwon No. 8) fumigated by $SO_2$ were investigated inside a phytotron. The results were as follows: 1. With the increase of the $SO_2$ dosage, visible injury and the degradation of chlorophyll increased. 2. The degradation of chlorophyll b was less than that of chlorophyll a and the carotinoid was more easily degratated than chlorophyll b. 3. The degradation of chlorophyll by $SO_2$ fumigation was induced directly by $SO_2$, itself. 4. Light is necessary to the degradation of chlorophyll. $SO_2$ and $O_2^-$ made in the photolysis process of the water molecule were the major components in the degradation of chlorophyll.

• Applied Chemistry for Engineering
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• v.25 no.3
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• pp.324-329
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• 2014

Microalgae Nannochloropsis oculata (N. oculta) and Chlorella vulgaris (C. vulgaris) are important sources for biodisel because of the high content of neutral lipids. Stable nano bubble is maintained for a long time and therefore it is possible for use in biotechnology. In this study, effects of nano bubble oxygen or hydrogen water on the microalgae growth were characterized. The cell growth in nano bubble water was similar to that of control, and the total lipid content was rather low. But, chlorophyll content of N. oculata grown in nanno bubble oxygen and hydrogen water increased 54% and 30%, and increased 59%, 39% in C. vulgaris. Carotenoid content also increased 21%, 25% in N. oculata and 49%, 29% in C. vulgaris grown in nano bubble oxygen and hydrogen water. From these results, nano bubble water seems to enhance the photosynthetic capacity of microalgae.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.4
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• pp.285-296
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• 2014

The impacts of high temperature and drought were studied on the seedlings of three families (superiorgangwon74, intermediate-gangwon77 and inferior-gangwon132) of P. densiflora which had been selected by the based on the growth indexes of 32-year-old. The seedlings were grown in controlled-environment growth chambers with combinations of four temperatures ($-3^{\circ}C$, $0^{\circ}C$, $+3^{\circ}C$ $+6^{\circ}C$; based on the monthly average for 30 years in Korea) and two water conditions (control, drought). The growth performance, photosynthetic parameters and photosynthetic pigment contents were measured at every 30 days under four temperatures and drought condition, and the end of each treatment. The superior family showed higher relative diameter at root collar growth rate and the dry weight than intermediate and inferior family in all treatments. Under elevated temperature and drought condition, growth rate was decreased, and seedlings showed lower growth rate than that of control in three families under low temperature. Photosynthetic rate, stomatal conductance and transpiration rate of three families decreased with the increase of temperature and drought condition, and that of seedlings under low temperature was lower than control. But under elevated temperature and drought condition, water use efficiency increased in three families. Photosynthetic pigment contents of leaves decreased under the increase of temperature and drought condition, but chlorophyll a/b ratio increased with the increase of temperature and drought condition in three families. The superior family showed higher total chlorophyll content than intermediate and inferior family in all treatments. In conclusion, P. densiflora is under changed temperature and drought condition, growth was decreased, seedlings more affected in elevated temperature than that of decreased temperature. The increase in monthly average temperature in Korea of more than $6^{\circ}C$, P. densiflora seedling growth in depending on region may decrease. In this study, the superior family(gangwon74) showed more excellent growth and physiological responses than intermediate (gangwon77) and inferior(gangwon132) family under changes temperature and drought.

• Korean Journal of Environmental Biology
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• v.18 no.4
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• pp.425-440
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• 2000

The physico-chemical characteristics and the concentrations of chlorophylls of coastal seawater were investigated to know the seasonal variations of biological oceanographic environments in the Islands of Ullungdo(U) and Dokdo(D). The samplings of sea water according to different depths were performed four seasons (May, June, August and November) in five stations along the coast of Ullungdo Island and 3 times (June, August and November) in three stations around the coast of Dokdo Island. The seasonal variations of sea water temperature showed that the formation of thermocline in August was distinct in comparison to the other seasons. The sea water in the surface was influenced by low temperature-high salinity in May and with high temperature-low salinity in the investigated area. The amount of seston was high in May (5.3-15.0mg/l) and was low in August (1.4-4.9mg/l) in ullungdo island. for the nutrients or sea water in Ullungdo Island, the concentrations of nitrate and ammonium were higher than Dokdo Island (nitrate-max. of U in August : 0.10-11.50$\mu\textrm{g}$/1, max. of D in August : 2.92-8.10$\mu\textrm{g}$/l : ammonium-max. of U in November : 14.18-20.69$\mu\textrm{g}$/l, max. of D in June : 0-1.78 $\mu\textrm{g}$/l). The high concen-tration of chlorophylls showed on the deeper layer from 30 m to 50 m in August (U 30 m : 0.85$\mu\textrm{g}$/l ; D 50m : 1.02 $\mu\textrm{g}$/l), while the concentrations of chlorophylls were even in May, June and November in the deeper layer of surface layer. In conclusion, the establishment of thermocline in deeper area of the euphotic layer in August was a trigger far the development of phytoplankton, while the complex physico-chemical system by diverse currents and vertical mixing of sea water in the area induced the even distribution of phytoplankton in both epilimnion and hypolimnion in May, June and November.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.404-415
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• 2020

We investigated the seasonal variations in growth and physiological responses of the kelp species Ecklonia cava to different nitrogen sources to establish indoor culture conditions for mass production. Ecklonia cava was cultivated for 10 days in 16 combinations of seawater temperatures (15, 17, 21, and 25℃) and different nitrogen sources (control; NH-NH₄⁺ 100 μM; NO-NO₃^- 100 μM; and NHNO-NH₄⁺ 50 μM+NO₃^- 50 μM). The growth and growth rate of the blade were affected by temperature. The mean fresh weight and area-based daily growth rate were the highest (5.8±0.5 and 6.6±0.5% day^-1, respectively) at 15℃ and the lowest (2.2±0.2 and 3.0±0.3% day^-1, respectively) at 25℃. The daily growth rate was the highest in the NH and NO treatments and lowest in the control. The nitrate reductase activity of E. cava varied with water temperature (season). The highest activity was in the control (1.32±0.10 μmol NO₂^- g^-1 dry weight h^-1) and the lowest was in the NH treatment (0.25±0.02 μmol NO₂^- g^-1 dry weight h^-1). The photosynthetic pigment concentrations reached a maximum value in the NHNO treatment and a minimum value in the control. These results showed that water temperature played an important role in the cultivation of E. cava and that a single supply of NH₄⁺ or NO₃^- may induce the accelerated growth of E. cava. The growth and physiological responses of E. cava to different nitrogen sources during each season provide valuable information for determining the optimal nitrogen source in E. cava cultivation under indoor conditions.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.126-133
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• 2019

In recent years, the deterioration of fruit quality caused by poor coloration and sunburn disorder has become serious problems in apple market, which is a result of the increase of surface temperature due to the abnormal temperature increase during summer season. This study was conducted to investigate the effect of wind net shading and fine water spray using sprinkler on fruit coloration, sunburn damage and overall fruit quality of 'Fuji' and 'Hongro' apples. Fifteen sprinklers (7L/hr) were installed at the orchard of the Chungcheongnam-do Agricultural Research and Extension Services, located in Sinam-myeon Chungcheongnam-do Korea, at a height of 3m above the apple tree of $1.5m{\times}3.5m$ north-south direction. Fine water spraying treatment was divided into day time spray (10:00 am to 6:00 pm) and all day spray (10:00 am to 10:00 pm) from early July to 10 days before harvest in 2017 and 2018 season, respectively. Temperature of the surface of apple fruit, characteristic of fruit, and degree of sunburn damage were investigated. In 'Fuji', the fruit surface temperature checked at 2 pm on August 10 was decreased considerably in the day time spray ($35.6^{\circ}C$) and wind net ($39.0^{\circ}C$) when compared with the untreated control ($44.4^{\circ}C$). Similarly, the fruit surface temperature also decreased considerably in the all day spray ($35.1^{\circ}C$) and wind net ($36.9^{\circ}C$) treatments when compared with the untreated control ($46.5^{\circ}C$) in 'Hongro' apples. The incidence of sunburn disorder was significantly decreased with day time spray (5.0%), all day spray (5.8%) and wind net (7.0%) when compared with untreated control (23.4%) in 'Fuji' apples. As a results, the treatment of fine water spray and wind net consequently showed 26% and 34% increase of redness ($a^*$) value in the skin color difference, respectively, in 'Fuji' apples.

• Korean Journal of Ecology and Environment
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• v.44 no.3
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• pp.303-313
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• 2011

Field observations and culture experiments have been carried out during the rainy season (on the 6th, 8th and 27th July 2009) to examine changes in the primary productivity and associated plant pigments in the estuary of the Seom-jin River. Primary productivity was determined at four sampling stations along the salinity gradient. On 6th July (before heavy rain) primary productivity ranged from 689~1,169 mgC $m^{-2}$ $d^{-1}$. On the 8th, just after more than 216.5 mm of precipitation, euphotic layers at all stations were reduced to very shallow water because of the high concentration of suspended solids in the water. This resulted in dramatically decreased primary productivity down to as low as 12~32 mg C $m^{-2}$ $d^{-1}$. However, after the rain, primary productivity on the 27th ranged from 266~999 mgC $m^{-2}$ $d^{-1}$, demonstrating a fast recovery in the upper stream water to similar productivity levels to those before the rainy season. Concentration of fucoxanthin in the water was highest on the 6th July. Before the rain, concentration of the zeaxanthin, increased as the salinity decreased. Immediately after the heavy rain, the Chl b (Chlorophytes) concentration was higher at all sites than before the rainy season. The concentration of fucoxanthin decreased after the heavy rain. At the downstream site, peridinin (Dinoflagellates) were found. During the rainy season, the diatoms contributed to the primary productivity at all sites. However, after the rainy season, Chl b (Chlorophytes) and Peridinin (Dinoflagellates) increased, demonstrating the enhanced contribution of those species in addition to diatoms.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.2
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• pp.101-110
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• 2013

In order to classify the trophic state and environmental quality of marine coastal system, an approach using the characteristics and biochemical composition in the sediments can be available. This research, including 25 coastal bay, belong to 131 stations, was carried out along the south coasts of Korea in February 2007. Type of sediment, total ogranic carbon, total nitrogen, phytopigments and biochemical composition(proteins, lipids, carbohydrates) were analyzed. Result from Multi-dimensional Scaling(MDS) ordination indicates that four group can be identified. The result of ANOVA with tukey test shows that the concentrations of proteins, carbohydrates and biopolymeric carbon were significantly different to four groups. We propose the trophic state classification for these groups using the biochemical composition of sediment organic matter. I group(Masan, Jinhae, Haengam) has been defined as hypertrophic state, II group(Tongyeong, Goseong；Jaran, Geoje et al.), as eutrophic; III group(Gamak, Deungnyang, Yeoja et al.), as mesotrophic and IV group(Sinan, Jindo, Muan), as oligotrophic. On the basis of results reported in this study, the biochemical composition of sediment organic matter could be considered an useful and sensitive tool for the classification of the trophic state of marine coastal systems.