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Several Factors Affecting Growth of Veronica rotunda var. subintegra (Nakai) T. Yamaz. Stem Cutting (산꼬리풀 삽수의 생장에 미치는 몇 가지 요인)

  • Oh, Hye Jin;Lee, Seung Youn;Shin, Un Seop;Kim, Hee Chae;Kim, Sang Yong
    • Korean Journal of Plant Resources
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    • v.34 no.4
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    • pp.270-277
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    • 2021
  • Veronica rotunda var. subintegra (Nakai) T. Yamaz. is perennial herb belonging to the family Scrophulariaceae, native to Korea. Plant growth regulators, cutting mediums, cutting position, and leaf size were examined to investigate the requirements for cutting propagation of V. rotunda var. subintegra. This study was conducted over a period of 6 weeks from March to May 2017 in the Division of Plant Resources, Korea National Arboretum. For all treatments, survival and rooting percentage was 100%. In PGRs experiment, the number of roots and shoot length treated with 100 mg/L IBA treatment was significantly higher than that of NAA treatment. The shooting percentage was decreased with an increase of the PGRs concentration. In growing substrates experiment, the number of roots on commercial soil (61.2) was the highest, followed commercial + kanuma soil (45.7), and the lowest was kanuma soil (41.8). The shoot length treated with commercial soil (8.9 cm) was significantly greater than that of kanuma soil (4.1 cm). In cutting positions experiment, the number of roots treated with apical (56.6) was significantly higher than that of basal (38.8). In leaf-cut experiment, the number of roots and of shoot length treated with full leaf was significantly higher than that of half leaf. As a result, we recommend treating cuttings with 100 mg/L IBA treatment, commercial soil, apical position, and full leaf for successful cutting propagation of V. rotunda var. subintegra.

Mass Propagation of Hypolepis punctata (Thunb.) Mett. Using In Vitro Culture Techniques (조직배양을 이용한 점고사리의 대량증식 방법)

  • Park, Kyungtae;Jang, Bo Kook;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.57-57
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    • 2019
  • 본 연구는 관상 및 조경용으로 개발이 가능한 남방계 양치식물인 점고사리[Hypolepis punctata (Thunb.) Mett.]의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 무가온 온실에서 성숙한 포자엽을 채집한 후 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 8주 간격으로 계대배양 하여 실험의 재료로 사용하였다. 배지종류에 따른 전엽체의 증식 및 형태형성을 확인하고자, 배양된 전엽체 0.3g을 메스로 잘게 다진 후, 농도를 1/4, 1/2, 1, 2배로 조절한 MS배지에 8주간 배양하였다. 이후 선발된 배지를 기준으로 sucrose, 활성탄, 질소급원의 농도를 조절하여 전엽체의 증식과 형태형성을 확인하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 초기 접종량인 0.3g에 비해 10.7배 증가한 3.2g으로 가장 높은 증가율을 보였다. 형태관찰에서도 장정기의 형성이 관찰되었으며, 전엽체의 쿠션조직이 비교적 잘 발달하였다. 전엽체 증식에 가장 좋은 효과를 보인 1MS배지를 기준으로 sucrose의 농도를 0-4%로 달리하여 실험한 결과, 1%의 처리구에서 6.7g으로 가장 높은 생체중을 보였다. 활성탄의 농도를 0-0.8%로 첨가한 네 처리구 중에서는 0.8%의 처리구에서 14.2g으로 무처리구에 비해 생체중이 2배 이상 증가하였다. 전엽체의 형태 또한 정상적인 발달을 보였다. 질소급원의 비율을 30-120mM로 조절한 배지에서는 60mM의 처리구에서 4.9g으로 가장 높은 생체중을보였다. 이후 포자체 형성을 위한 최적의 토양조건을 구명하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 달리하여 5종류의 배양토를 조성하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진 하였으며, 배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 준비된 토양표면에 분주하여 재배하였다. 12주간의 재배 결과, 원예상토를 단용한 토양에서 포자체의 수가 포트당 250.0개로 가장 많이 형성되었으며, 포자체의 생육 또한 다른 처리구에 비해 우수한 결과를 보였다. 한편 피트모스가 혼합된 토양에서는 포자체가 형성되지 않았다. 따라서 점고사리의 전엽체 대량증식에 적합한 배지는 sucrose 1%와 질소급원의 농도를 60mM, 활성탄을 0.8% 첨가한 1MS배지로 판단되며, 포자체 대량생산을 위해서는 원예상토를 단용한 토양이 적합하다고 판단된다.

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Effects of Heavy Metal Concentrations on Seed Germination and Rhizome Development of Campanula takesimana Nakai (중금속 농도가 섬초롱꽃 종자 발아 및 유근 발달에 미치는 영향)

  • Kim, Tae Hyun;Kim, Do Hyun;Im, Hyeon Jeong;Choi, Kyu Seung;Kim, Sang Geun;Song, Chi Hyeon;Oh, Beom Seok;Kim, Yang Su;Song, Ki Seon;Won, Chang O
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.39-39
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    • 2019
  • 섬초롱꽃은 울릉군에 한정적으로 분포하는 여러해살이풀이다. 산림청에서는 약관심종(LC) 등급의 희귀식물이자 특산식물로 분류하고 있으며, 꽃의 관상가치가 특히 높아 화단용 소재, 절화용, 화분재배 등으로 흔히 이용되고 있다. 따라서 본 연구는 섬초롱꽃의 대량증식 시, 중금속이 미칠 수 있는 영향에 대해 파악하고자 수행하였다. 실험에 사용한 중금속은 카드뮴(Cd), 철(Fe) 두 종류이며, 두 종류 모두 1% 한천(agar)배지에 10, 25, 50, 100, 200 mg/L 농도로 녹여 사용하였다. 발아율 및 유근 생장을 위해 종자 및 유묘를 치상 후, $25^{\circ}C$ 항온 생장상에서 발아율 및 유근 발달을 관찰하였다. 발아율 측정결과, 아무처리도하지 않은 1% 한천(agar) 배지에서 $62.7{\pm}7.4%$로 가장 높았고 중금속 농도가 높아질수록 감소하였다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $17.3{\pm}1.3$, $2.7{\pm}1.3$, $1.3{\pm}1.3$, 0, 0%로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $46.7{\pm}4.8$, $24.0{\pm}2.3$, $36.0{\pm}6.9$, $20.0{\pm}2.3$, 0%로 나타났다. 유근의 평균 생장 길이(mm)는 아무처리도 하지 않은 1% 한천(agar) 배지에서 19.6 mm로 가장 높았고, 중금속 농도가 높아질수록 낮게 나타났다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 4.6 mm, 1.9 mm, 0.7 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 15.9 mm, 8.6 mm, 8.1 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 실험결과를 종합해 볼 때, 섬초롱꽃 종자 발아 및 유근 생장의 억제는 카드뮴(Cd) 처리구에서 높게 나타났고, 철(Fe) 처리구에서는 비교적 영향을 덜 미치는 것으로 나타났다. 특히 카드뮴은 10 mg/L 농도만 처리하더라도 약 3.6~4.2배의 발아율 생장율 감소가 나타나, 섬초롱꽃 대량증식 시, 치명적일 것으로 판단된다. 본 실험의 결과는 섬초롱꽃 종자를 이용한 대량증식 및 유묘재배에 있어, 토양을 선택하는데 중요한 기초자료로서 활용될 것으로 기대된다.

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The Developing Trend of Cardiovacular Drug Coated Balloon Catheter (심장혈관용 약물코팅풍선카테터의 개발 동향)

  • Park, Junghun;Jo, Won-Il;Byeon, Dae-Heung;Kang, So Young;Nam, Choong-hyun;Seo, Kyoung-Woo;Park, Jun-Kyu
    • Applied Chemistry for Engineering
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    • v.33 no.5
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    • pp.545-550
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    • 2022
  • As a result of the rapid entry into an aging society and westernized eating habits, the number of heart disease patients caused by angina pectoris, myocardial infarction, and high blood pressure has increased by more than 100,000 over five years. Heart disease has consistently ranked second in the cause of death in Korea over the past eight years, and medical expenses consumed annually reach 6 trillion won. While various treatments are being proposed for more patients, drug-coated balloon catheters have been developed and gradually commercialized to solve the disadvantages of stent implantation such as restenosis and increased risk of bleeding due to long-term double antiplatelet medication. In Korea, it began to use a drug-coated balloon catheter with the first release of it called "SeQuent Please (Bibrown Korea)" in 2010. Its demand increased gradually as insurance benefits were applied in 2012. Drug-coated balloon angioplasty is increasing in use not only in Korea but also around the world, especially in the Asia-Pacific region, including Japan. Until now, the demand for domestic products is increasing, and if the efficiency in vivo and clinical trials is proven in the future, it is expected to be an effective procedure compared to high-risk stent implantation.

Flowability and Strength of Self-compacting Concrete Mixed with Tailings from the Sangdong Tungsten Mine (상동관상 광미를 혼합한 자기충전콘크리트의 유동 및 강도 특성)

  • Choi, Yun Wang;Kim, Yong Jic;Jung, Moon Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4A
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    • pp.767-774
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    • 2006
  • This study has focused on the possibility for recycling of tailings from the sangdong tungsten mine as powder (TA) of self-compacting concrete (SCC). The experimental tests for entrapped water ratio were carried out in accordance with the specified method by Okamura. The rheological measurements of cement paste were conducted by using a commercially digital Brookfield viscometer (Model LVDV-II+) equipped with cylindrical spindles, also tests for slump-flow, time required to reach 500 mm of slump flow (sec), time required to flow through V-funnel (sec) and filling height of U-box test (mm) were carried out in accordance with the specified by the Japanese Society of Civil Engineering (JSCE). The results of this study, entrapped water ratio was decreased with increasing replacement of TA. Thickness of pseudo water film was increased, and mean plastic viscosity was decreased with increasing replacement of TA. And slump-flow of SCC was decreased with increasing replacement of TA. But time required to reach 500 mm of slump flow (sec), time required to flow through V-funnel (sec) and filling height of U-box test (mm) were satisfied a prescribed range. The mechanical properties including compressive strength, splitting tensile strength and elastic modulus were checked with the requirements specified by Korean Industrial Standard (KS). The compressive strength of SCC was decreased with increasing replacement of TA, splitting tensile strength and elastic modulus were similar to those of normal concrete.

Optimal Conditions for Groundcovers Cultivation of Korean Native Lythrum salicaria L. (자생 털부처꽃의 지피용 재배를 위한 최적 조건)

  • Bo Kook Jang;Soo Ho Yeon;Ju Sung Cho;Cheol Hee Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.24-24
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    • 2020
  • 본 연구는 자생 털부처꽃의 실용적인 산업화를 위해 지피용 재배조건을 조사하고자 수행되었다. 실험재료는 털부처꽃의 종자를 2019년 4월말에 파종하여 8주 후 얻어진 유묘를 사용하였다. 실험은 식재용기(2, 3, 4치 비닐포트), 토양종류(원예상토 단용 및 마사토:피트모스 혼용토(v/v; 2:1, 3:1), 파종용기(162, 200, 288셀 플러그트레이) 및 파종립수(1, 2, 4립)를 달리하였으며, 정식 후 처리구간의 생육특성을 조사하였다. 또한 마사토와 피트모스 혼용토는 9L당 퇴비 1L를 혼합하여 원예상토와 비교하였다. 대조구는 원예상토가 충진된 3치 비닐포트에 정식된 유묘와 셀당 1립이 파종된 200셀 플러그트레이의 유묘를 사용하였다. 유묘는 처리별로 정식한 다음 충북대학교 유리온실에서 12주간 재배되었으며, 매일 2회(5분/1회) 미스트 관수되었다. 연구결과, 털부처꽃의 유묘가 정식된 비닐포트는 포트의 볼륨이 클수록 생육이 우수한 경향을 나타냈다. 특히 4치 비닐포트는 동일한 재배기간 동안 경직경(3.61 mm), 측지수(28.2 ea), 엽수 (311.6 ea), 근장 (22.1 cm) 및 생체중(7049.9 mg)이 가장 많이 증가하였다. 토양종류는 원예상토를 단독으로 사용한 처리구의 생육이 가장 우수하였다. 반면 마사토와 피트모스 혼용토는 재배가 지속되면서 생육이 저조하여, 농업용 퇴비로는 양분이 부족한 것으로 보인다. 파종립수가 증가할수록 각 개체의 생육은 감소하였으나, 4립 파종구의 유묘는 정식 후 풍성한 볼륨을 나타냈으며, 지피용 재배목적에 부합하였다. 플러그트레이는 볼륨이 작아질수록 초장과 생체중이 일부 감소하는 경향을 나타냈으나, 전반적으로 생육은 유사한 경향을 나타냈다.

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Flora of Mt. Balbaek (Gyeongbuk, Gyeongsan) (발백산(경북, 경산)의 관속식물상)

  • Youjin Hwang;SeongJun Park;SuMi Choi;Saeyeon Hwang;Jaesik Hwang;Seonghyun Bae;SeonJoo Park
    • Korean Journal of Plant Resources
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    • v.36 no.1
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    • pp.62-90
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    • 2023
  • This study was conducted to study the vascular plants of Balbaeksan, located on the border between Gyeongsan-si and Cheongdo-gun, Gyeongsangbuk-do. It was performed 13 times from September 2020 to November 2021, and the investigated vascular plants were identified as a total of 93 families, 302 genera, 485 species, 10 subspecies, 40 varieties, 13 varieties, and 545 taxa. Endemic plants were investigated in 18 taxa, 9 taxa of rare plants; Vulnerable (VU) 5 taxa, Least concerned (LC) 3 taxa, Data Deficient (DD) 1 taxa, Red List plants 5 taxa; 3 taxa of least concerned (LC), 2 taxa of Not Evaluated (NE), 183 taxa of biological resources subject to export approval, 78 taxa of specific taxonomy; There were 9 taxa of grade IV, 20 taxa of grade III, 20 taxa of grade II, and 28 taxa of grade I. 4 taxa of plants subject to climate change adaptation; 2 taxa of endemic plants, 1 taxa of southern plants, 1 taxa of northern plants, 4 taxa of climate change biomarker species, 2 taxa of candidate species of climate change biomarker, 65 taxa of exotic plants, and 3 taxa of ecosystem disturbance species. Plants 235 taxa (43.1%), medicinal plants 257 taxa (47.2%), ornamental plants 148 taxa (27.2%), woody plants 54 taxa (9.9%), herbaceous plants 37 taxa (6.8%), industrial plants 15 Taxa (2.8%), 11 taxa (2.0%) of fiber-bearing plants, and plants of unknown use were identified as 73 taxa (13.4%). The flora of Mt. Balbaek was investigated for the first time, and plants to be preserved in order to secure useful plant resources and biological sovereignty were identified. So continuous monitoring is necessary to prevent the introduction of foreign plants that may be caused by the recent increase in the number of people coming and going.

An Investigation of Local Naming Issue of Tamarix aphylla (에셀나무(Tamarix aphylla)의 명칭문제에 대한 고찰)

  • Kim, Young-Sook
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.37 no.1
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    • pp.56-67
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    • 2019
  • In order to investigate the issue with the proper name of eshel(Tamarix aphylla) mentioned in the Bible, analysis of morphological taxonomy features of plants, studies on the symbolism of the Tamarix genus, analysis of examples in Korean classics and Chinese classics, and studies on the problems found in translations of Korean, Chinese and Japanese Bibles. The results are as follows. According to plant taxonomy, similar species of the Tamarix genus are differentiated by the leaf and flower, and because the size is very small about 2-4mm, it is difficult to differentiate by the naked eye. However, T. aphylla found in the plains of Israel and T. chinensis of China and Korea have distinctive differences in terms of the shape of the branch that droops and its blooming period. The Tamarix genus is a very precious tree that was planted in royal courtyards of ancient Mesopotamia and the Han(漢) Dynasty of China, and in ancient Egypt, it was said to be a tree that gave life to the dead. In the Bible, it was used as a sign of the covenant that God was with Abraham, and it also symbolized the prophet Samuel and the court of Samuel. When examining the example in Korean classics, the Tamarix genus was used as a common term in the Joseon Dynasty and it was often used as the medical term '$Ch{\bar{e}}ngli{\check{u}}$(檉柳)'. Meanwhile, the term 'wiseonglyu(渭城柳)' was used as a literary term. Upon researching the period and name of literature related to $Ch{\bar{e}}ngli{\check{u}}$(檉柳) among Chinese medicinal herb books, a total of 16 terms were used and among these terms, the term Chuísīliǔ(垂絲柳) used in the Chinese Bible cannot be found. There was no word called 'wiseonglyu(渭城柳)' that originated from the poem by Wang Wei(699-759) of Tang(唐) Dynasty and in fact, the word 'halyu(河柳)' that was related to Zhou(周) China. But when investigating the academic terms of China currently used, the words Chuísīliǔ(垂絲柳) and $Ch{\bar{e}}ngli{\check{u}}$(檉柳) are used equally, and therefore, it appears that the translation of eshel in the Chinese Bible as either Chuísīliǔ (垂絲柳) or $Ch{\bar{e}}ngli{\check{u}}$(檉柳) both appear to be of no issue. There were errors translating tamarix into 'やなぎ(willow)' in the Meiji Testaments(舊新約全書 1887), and translated correctly 'ぎょりゅう(檉柳)' since the Colloquial Japanese Bible(口語譯 聖書 1955). However, there are claims that 'gyoryu(ぎょりゅう 檉柳)' is not an indigenous species but an exotics species in the Edo Period, so it is necessary to reconsider the terminology. As apparent in the Korean classics examples analysis, there is high possibility that Korea's T. chinensis were grown in the Korean Peninsula for medicinal and gardening purposes. Therefore, the use of the medicinal term $Ch{\bar{e}}ngli{\check{u}}$(檉柳) or literary term 'wiseonglyu' in the Korean Bible may not be a big issue. However, the term 'wiseonglyu' is used very rarely even in China and as this may be connected to the admiration of China and Chinese things by literary persons of the Joseon Dynasty, so the use of this term should be reviewed carefully. Therefore, rather than using terms that may be of issue in the Bible, it is more feasible to transliterate the Hebrew word and call it eshel.

Limitation of Prediction on Intravenous Immunoglobulin Responsiveness in Kawasaki Disease (가와사끼병에서 정맥용 면역글로불린 치료 반응 예측의 한계)

  • Kim, Seong-Koo;Han, Ji-Yoon;Rhim, Jung Woo;Oh, Jin Hee;Han, Ji-Whan;Lee, Kyung Yil;Kang, Jin-Han;Lee, Joon-Sung
    • Pediatric Infection and Vaccine
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    • v.17 no.2
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    • pp.169-176
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    • 2010
  • Purpose : We aimed to evaluate predictive parameters for non-response to intravenous immunoglobulin (IVIG) in patients with Kawasaki disease (KD) before IVIG use using two controls. Methods : We evaluated 229 consecutive KD patients who were treated with 2 g/kg of IVIG at a single center. Those who had persistent fever >24 hours after IVIG infusion made up the 23 IVIG non-responders; the first control included a total 206 defervesced cases and the second control included 46 cases that were matched for age and pre-treatment fever duration to non-responders. Results : Demographic and clinical characteristics were similar in IVIG non-responders and responders at presentation. As for laboratory findings, the neutrophil differential, CRP, AST, ALT, and LDH were higher, and lymphocyte differential, total protein, albumin, platelet count, and total cholesterol were significantly lower in IVIG non-responders compared to responders by univariate analysis in both study designs. However in multivariate analysis, non-responders showed a significantly higher neutrophil differential (cutoff value, >77%, sensitivity 68.4% and specificity 79.5%) and lower cholesterol (<124 mg/dL, sensitivity 79% and specificity 70.5%). Whereas plasma albumin (<3.6 g/dL, sensitivity 73.7% and specificity 60%) was the sole laboratory parameter of non-responders in the second study design. Conclusion : Severity of inflammation in KD was reflected by higher or lower laboratory values at presentation. Because the multivariate analysis for these indices may be influenced by some confounding factors, including the numbers of patients of different ages and fever duration, other assessment modalities are needed for KD patients with the greatest risk of coronary artery lesions.

The characteristic laboratory findings of non-responsiveness to intravenous immunoglobulin in children with Kawasaki disease (가와사끼병 재 치료군의 특징적인 검사 지표)

  • Cho, Han Gil;Cho, Young Kuk;Ma, Jae Sook
    • Clinical and Experimental Pediatrics
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    • v.53 no.2
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    • pp.228-234
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    • 2010
  • Purpose : Although intravenous immunoglobulin (IVIG) treatment is an effective first-line treatment for Kawasaki disease, 10-20% of the patients develop persistent fever or coronary artery complications. Medical records of Kawasaki disease patients were reviewed to assess the characteristic laboratory findings of IVIG nonresponsiveness. Methods : We reviewed the clinical records of 118 children with Kawasaki disease who were treated at the Chonnam National University Hospital from March 2003 to February 2008. The laboratory findings of the IVIG-responder group (n=110) and the IVIG-nonresponder group (n=8) were compared at admission day and at 48 hours and 14 days after IVIG administration. Results : At admission, the level of creatine kinase (CK) was lower (P =0.03) and that of total protein was higher (P <0.01) in the nonresponders than in the responders. At 48 hours after IVIG administration, the white blood cell (WBC) count (P =0.04) and neutrophil% (P <0.01) was higher in the nonresponders than in the responders. The neutrophil% (P <0.01) and CK (P =0.01) level at admission was lower than that at 48 hours after IVIG administration in the responders; this decrease was not as apparent in the nonresponders. Conclusion : IVIG nonresponders have lower CK and higher total protein levels at admission and higher WBC count and neutrophil% at 48 hours after IVIG administration. The decrease in the neutrophil% and CK level between at admission and at 48 hours after IVIG administration is remarkably higher in responders than in nonresponders.