• Journal of Life Science
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• v.22 no.7
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• pp.871-879
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• 2012

Polyploids are a potentially important germplasm source in seedless citrus breeding program. Seedlessness is one of the most promising traits of commercial mandarin breeds that mandarin triploid hybrids possess permanently. The formation of new constant triploid hybrids can be recovered through diploid species hybridization from the fusion of divalent gametes at low frequencyor intra-and inter-ploidy crosses. However, extensive breeding work based on small $F_1$ hybrid seeds developed is impossible without a very effective aseptic methodology and ploidy event. In this study, in vitro embryo culture was employed to recover natural hybrids from monoembryonic diploid, open-pollinated mandarin. Flow cytometry was used to determine ploidy level. A total of 10,289 seeds were extracted from 792 fruits having approximately 13 seeds per fruit. Average frequency of small seeds developed was 7.1%, while the average frequency of small seeds per fruit were: 8.9% for 'Clementine' 10.2% for 'Harehime' 2.6% for 'Kamja' 3.1% for 'Pyunkyool' 2.8% for 'Sadookam' and 7.0% for 'Wilking' mandarin. Average size of a perfect seed was $49.52{\pm}0.07mm^2$ ('Clementine') while the small seed measured $7.95{\pm}0.04mm^2$ ('Clementine'), which was about 1/6 smaller than the perfect seed. In total, 731 small seeds were obtained and all of them contained only one embryo per seed. The efficiency of 'Clementine' was 14 times higher than 'Wilking' and more than 109 times higher than 'Pyunkyool'. The basic information on spontaneous polyploidy provides for the hybridization of constant triploids and increases the efficiency of conventional cross.

• 한국방재학회:학술대회논문집
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• 2011.02a
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• pp.63-63
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• 2011

화재란 사람의 의도에 반하거나 고의에 의해 발생하는 연소현상으로서 소화시설 등을 사용하여 소화할 필요가 있는 것을 말한다. 사람의 의도에 반한다고 하는 것은 과실에 의한 화재를 의미하며 화기취급 중 발생하는 실화뿐만 아니라 부작위에 의한 자연발화도 포함하며, 고의에 의한다고 하는 것은 일정한 대상에 대하여 피해발생을 목적으로 화재발생을 유도하였거나 직접 방화한 경우를 말한다. 연소현상이라 함은 가연성 물질이 산소와 결합하여 열과 빛을 내며 급속히 산화되어 형질이 변경되는 화학반응을 말한다. 소화시설 등을 사용하여 소화할 필요가 있다는 것은 화재란 연소현상으로서 소화의 필요성이 있어야 하며 소화의 필요성의 정도는 소화시설이나 그와 유사한 정도의 시설을 사용할 수준 이상이어야 한다. 화재원인조사란 발화부를 판단하고 화재에 이르게 된 발화원을 규명하며, 발화부로부터 연소확대된 경과를 조사하는 일련의 행위로서 화재원인조사시 가장 중요한 사항은 발화부 판단인 바, 이는 화재원인이 발화부에만 존재하기 때문이다. 최근에는 다양한 소재의 사용으로 인해 일단 화재로 진행될 경우 인명 및 재산상 피해가 증가하게 되어 있으며, 이로 인해 화재조사시 화학, 물리, 전기, 건축, 기계, 소방 등 다양한 지식과 화재현장에 대한 이해가 요구된다. 화재현장 조사시 발화부 판단의 과학적 접근은 매우 중요한 것으로서, 화재원인의 명쾌한 규명으로 책임한계 구분은 물론, 유사사고의 재발방지를 위한 연구가 필요하다. 본 연구에서는 기 발생한 화재사례에서 얻은 화재패턴을 분석하여 얻은 자료를 통해 발화부를 판단할 수 있는 조사방법을 다음과 같이 제시하였다. 1) 화재시 열전달과 화염확산 과정에서 건축구조물, 내장재, 집적물 및 각종 설치물의 구조, 재질 등에 따라 다양한 화재패턴을 형성하게 됨을 알 수 있다. 2) 화재패턴의 종류로는 화재플럼에 의한 삼각형, 주상, V, U 패턴 등이 있으며, 연소 생성물인 화염, 연기, 열 등에 의해 다양한 형태를 보임을 알 수 있다. 3) 위와 같은 결과를 종합하여 연소의 상승성, 불꽃 및 연기 흔적, 열에 의한 흔적 등에 의해 연소의 방향성을 알 수 있다. 4) 결국 화재현장에서 명확한 화재원인을 규명하기 위해서는 화재패턴에 의해 연소확대과정을 역으로 추적하여 발화부를 결정한 다음, 발화부내에서 화재원인을 찾아내야 할 것이다.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.345-358
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• 2008

The systematic position of Cymopterus melanotiligia (H. Boissieu) C. Y. Yoon in Apiaceae was revaluated based on the ITS sequence data. Its diagnostic characteristics include its asymmerty mericarps, 3-4 mericarp ribs, winged dorsal ribs, and ternate leaves. It is morphologically similar to Tilingia tsusimensis and Pimpinella having ternate leaves. It is distinguished from Ostericum having symmerty mericarps, 5 mericarp ribs, and rounded dorsal ribs. Also it looks like Angelica with broadly winged lateral ribs and strongly dorsally compressed mericarps, but is distinguished by having its conspicuous calyx teeth and unilayer pericarps. Cymopterus melanotiligia formed a different clade group with Cymopterus species distributed in North America based on ITS sequences, and it supports the close relationships with Halosciastrum melanotilingia. Thus, we conclude that this species is here referred to Halosciastrum melanotilingia (H. Boissieu) Pimenov & Tikhomirov.

• Journal of Life Science
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• v.29 no.11
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• pp.1227-1234
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• 2019

Prunus mume, also known as maesil, is a popular fruit consumed in East Asia (Korea, Japan, and China). It contains high amounts of organic acids, minerals, and polyphenols and has been used as a medication for fever, vomiting, and detoxification. In this study, the anti-proliferative and apoptotic effects of solvent fractions from maesil were evaluated using sulforhodamine B (SRB) assays, morphological evaluations, Hoechst 33258 staining, and western blotting. Addition of the maesil methanol fraction (MMF) and the maesil butanol fraction (MBF) significantly and dose-dependently decreased the cell viability of HT-29 human colon cancer cells. Colony-forming assays confirmed that the MMF and MBF treatments decreased colony numbers when compared with untreated control cells. Treatment of HT-29 cells with MMF and MBF caused a distortion of the cell morphology to a shrunken cell mass. Treatment with MMF and MBF also dose-dependently increased nuclear condensation and the formation of apoptotic bodies in HT-29 cells. Treatment with MMF and MBF significantly and dosedependently increased the expression of Bax (a pro-apoptotic protein), caspase-3, and poly ADP-ribose polymerase (PARP) and decreased the expression of Bcl-2 (an anti-apoptotic protein). MMF significantly and dose-dependently inhibited cell proliferation induced by bisphenol A, an environmental hormone. Therefore, MMF may have potential use as a functional food and as a possible therapeutic agent for the prevention of colon cancer.

• Journal of the Korean Society of Food Culture
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• v.8 no.2
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• pp.139-146
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• 1993

The predeccessor of Danjanjinja was Myorak temple which is built in the 7th century. At that age, the Buddhist culture of Japan had highly prospected by transmitting Buddhism to Japan from Han peninsular On the other hand, the private god of Fujiwara family in Danjanjinja is Uchigami which is one of typical Japanese popular belief like Dangshin of Korean's. Through these historical background, it could by presumed that the Kakitsisai might be the original form of Korean Buddhist sacrificial offerings from ancient age. So this study on Kakitsisai what had handed down from generation to generation about for 1300 years help us to study and estimate the ancient dietary culture of Korean and Japanese. 1. Kakitsisai performed high filling method in the sacrificial offerings like Kasuga, Horyuji and Korea. 2. The patterns and colors of high filling offerings are various in Korea and Japan. 3. They used unpolished rice by ancient rice, and called red and black one. We can guess both of countries ate unpolished rice at that age. 4. They used many kind of ancient wild fruits and vegetables. We could recognize what the ancients had eaten the foods.

• Research in Plant Disease
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• v.13 no.3
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• pp.131-136
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• 2007

Occurrence of popcorn disease on mulberry fruits was surveyed in several locations in Korea from 2005 to 2007. The diseased fruits turned grayish white and changed to hard and black sclerotia during overwintering after falling onto the ground. Apothecia produced from overwintered sclerotia in the fields of mulberry trees were observed in early May. Two types of apothecia were produced from the sclerotia, which were cup-shaped or club-shaped. The fungus with cup-shaped apothecia was identified as Ciboria shiraiana, and that with club-shaped apothecia as Scleromitrula shiraiana. C. shiraiana occurred at the ratio of about 6 vs. 4 in the fields. Occurrence of the disease differed remarkably depending on years, locations, and varieties of mulberry trees investigated. Field survey of the disease incidence showed that Baekhahyeongsang, Dahojosaeng, Hasusang, a native variety and Sawonppong 22 were resistant to the disease, Cheongilppong, Daeseongppong, Guksang 20 and Turkey-D were moderately susceptible, and Cateneo, Moreji and Sanggwa were highly susceptible. Disease incidence in the fields mulched with nonwoven fabrics or plastic films was much lower than that in the non-mulched fields. Disease incidence in the fields mulched with rice straws was similar to that in the non-mulched fields.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.855-868
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• 2014

Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.37-45
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• 2012

To select bacterial strains with antifungal activity against an anthracnose fungal disease causing damage severely on hot pepper, previous isolates obtained from plant root samples were screened. Among 457 isolates, IA70-5 isolate was finally selected and identified as Streptomyces padanus based on 16S rDNA sequence analysis. Strain IA70-5 is non-pigmenteous, non-mobile, and filamentous. S. padanus IA70-5 inhibited effectively the mycelium growth, spore germination, and appressorium formation of Colletotrichum acutatum in vitro. The results of this study demonstrated that IA70-5 strain, especially applied on fruit of hot pepper, decreased disease incidence 90% for pre-inoculation before pathogen treatment. Taken together, S. padanus IA70-5 strain is a promising biological control agent to control of a major fungal pepper disease, anthracnose.

• Journal of Food Hygiene and Safety
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• v.22 no.3
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• pp.213-217
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• 2007

LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.