• Title/Summary/Keyword: 계통학적 연구

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Phylogenetic Study of Korean Chrysosplenium Based on nrDNA ITS Sequences (ITS 염기서열에 의한 한국산 괭이눈속(Chrysosplenium)의 계통학적 연구)

  • Han, Jong-Won;Yang, Sun-Gyu;Kim, Hyun-Jun;Jang, Chang-Gee;Park, Jeong-Mi;Kang, Shin-Ho
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.358-369
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    • 2011
  • The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from genus Chrysosplenium were sequenced to address phylogenetic relationship. ITS including 5.8S sequence varied in length from 647 bp to 653 bp. Among them, 219 sites were variable sites with parsimony-informative. The aligned sequences were analyzed by maximum parsimony (MP) and neighbor-joining (NJ) methods. In the strict consensus trees of parsimony analysis, the monophyly of Chrysosplenium was supported by 100% bootstrap value. The first clade, C. pseudofauriei was at the basal position of the genus, and others formed two clades with high bootstrap support. The second clade included Ser. Pilosa and Ser. Oppositifolia and third clade included Ser. Alternifolia and Ser. Flagellifera. The NJ trees showed essentially the same topology. Finally, DNA sequences of ITS regions were useful phylogenetic marker in this genus. Based on the ITS and ridge seed morphological results, C. sphaerospermum Maxim. and C. valdepilosum (Ohwi) S.H. Kang & J.W. Han were discussed their scientific names and taxonomic positions.

Study on Colored Rice -III. Major Growth Characteristics for the Promising Lines of Colored Rice Developed from Genetic Resources (유색미에 관한 연구 -III. 유색미 유망계통에 대한 주요 생육 특성)

  • Kim, Kwang-Su;Choi, Yun-Pyo;Kim, Sun-Taek;Choi, Hyun-Gu;Chung, Chong-Tae;Kim, Bo-Kyoung;Yu, Ji-Hong;Lee, Hee-Bong
    • Korean Journal of Agricultural Science
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    • v.34 no.2
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    • pp.125-129
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    • 2007
  • Aims of this study were carried out to develop the useful lines induced from mutation and pedigree breeding methods among the collected genetic resources from national and domestic areas. In this study, Stem height of CNU126 line and check among them were high, while CNU128 was lower than other lines. Spiklet length of CNU88 was longer, but that of CNU126 was shorter than check. Number of spiklets per plant of CNU50 among lines have twice time than check. 1,000 grains weight of CNU113 was higher than check. In yield per plant, CNU50 and CNU112 were higher than check, Dongjinbyeo.

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Analysis of Potential Toxigenicity and Phylogeny using Target Genes in Aphanizomenon flos-aquae (Cyanophyceae) strains isolated from the Nakdong River (낙동강에서 분리된 Aphanizomenon flos-aquae (Cyanophyceae) 균주의 목표 유전자를 이용한 잠재적 독소 생성능 및 계통학적 분석)

  • Ryu, Hui-Seong;An, Sung-Min;Lim, Chang-Kun;Shin, Ra-Young;Park, Jong-Guen;Lee, Jung-Ho
    • Korean Journal of Ecology and Environment
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    • v.50 no.1
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    • pp.137-147
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    • 2017
  • The identity of toxin producers remains only hypothesis unless there were identified by strain isolation and analytical confirmation of both the cyanotoxin production and the genetic identity of the monoculture. The purposes of this study were to identify a morphologic and phylogenetic classification in Aphanizomenon flos-aquae strains isolated from the Nakdong River and to investigate the potential ability of the strains to produce toxins such as saxitoxin and cylindrospermopsin using target genes. The 16S rRNA and sxtA, sxtI, cyrA, cyrJ genes were analyzed on two strains (DGUC001, DGUC003) isolated from the Nakdong River. Morphological features of the strains were observed a shape of aggregated trichomes in parallel fascicles which can reach up to macroscopic size and a hyaline terminal cell without aerotope. In addition, the 16S rRNA phylogenetic analyses showed that the strains were identified as the same species with high genetic similarity of 98.4% and grouped within a monospecific andsupported cluster I of Aphanizomenon flos-aquae selected from GenBank of the NCBI. The cyrA and cyrJ genes encoding for the cylindrospermopsin-biosynthesis were not detected in the present study. The sxtA gene was in detected both the two strains, whereas the sxtI gene which had been suggested as a suitable molecular marker to detect saxitoxin-producing cyanobacteria was not found both the strains. Thus, the two strains isolated from Nakdong River were identified as the same species of Aphanizomenon flos-aquae Ralfs ex Bornet et Flahault 1888, the two strains were confirmed as potential non-producing strains of the saxitoxin and cylindrospermopsin.

Morphological Characteristics and Genetic Diversity Analysis of Cultivated Sancho (Zanthoxylum schinifolium) and Chopi (Zanthoxylum piperitum) in Korea (국내 재배지의 산초(Zanthoxylum schinifolium)와 초피(Zanthoxylum piperitum)의 형태학적 특성과 유전적 다양성)

  • Ryu, Jaihyunk;Choi, Hae-Sik;Lyu, Jae-il;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.555-563
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    • 2016
  • The morphological characteristics and genetic relationships among 32 germplasms of Zanthoxylum schinifolium and Zanthoxylum piperitum collected from two farms in Korea were investigated. The traits with the most variability were seed color, leaf size, and spine size. The intraspecific polymorphism of Z. schinifolium and Z. piperitum was 96.5% and 60.3%, respectively. The genetic diversity and Shannon’s information index values ranged from 0.11 to 0.33 and 0.19 to 0.50, with average values of 0.26 and 0.42, respectively. Two ISSR primers (UBC861 and UBC862) were able to distinguish the different species. The genetic similarity matrix (GSM) revealed variability among the accessions ranging from 0.116 to 0.816. The intraspecific GSM for Z. schinifolium and Z. piperitum was 0.177-0.780 and 0.250-0.816, respectively. The GSM findings indicate that Z. schinifolium and Z. piperitum accessions have high genetic diversity and possess germplasms qualifying as good genetic resources for cross breeding. The clustering analysis separated Z. schinifolium and Z. piperitum into independent groups, and all accessions could be classified into three categories. Z. Schinifolium var. nermis belonged to independent groups. Comparison of the clusters based on morphological analysis with those based on ISSR data resulted in an unclear pattern of division among the accessions. The study findings indicate that Z. schinifolium and Z. piperitum accessions have genetic diversity, and ISSR markers were useful for identifying Z. schinifolium and Z. piperitum.

Anatomical Ultrastructure of Spermatozoa of Paralichthys olivaceus (Paralichthyidae, Perciformes) from Korea (한국산 넙치 (Paralichthys olivaceus) 정자의 미세해부학적 구조)

  • Kim, Kgu-Hwan;Lee, Byung-Chan;Nam, Myung-Mo
    • Korean Journal of Ichthyology
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    • v.23 no.2
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    • pp.106-110
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    • 2011
  • Fine structure of spermatozoa of the bastard halibut (Paralichthys olivaceus) is described. Its structure is characterized by acrosomeless, having a spherical head region and a small mid piece containing five mitochondria disposed one row. The flagellum inserts centrally on the nucleus and has lateral ribbons. The present study supports the suggestion that spermatozoal ultrastructure is of taxonomic importance and may be a phylogenetic criterion in teleostei.

Phylogeny of the Polygonatum odoratum Complex Inferred from Multiple cpDNA and Nuclear RNApol2_i23 Sequence Data (Ruscaceae) (엽록체 DNA 및 핵 DNA RNApol2_i23에 근거한 둥굴레복합체 (Ruscaceae)의 계통 연구)

  • Park, Jeong-Mi;Chung, Kyong-Sook;Oh, Byoung-Un;Jang, Chang-gee
    • Korean Journal of Plant Taxonomy
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    • v.41 no.4
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    • pp.353-360
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    • 2011
  • The sequence data of the plastid DNA (trnL-F IGS, trnL intron, and trnH-psbA) and nuclear DNA (RNApol2_i23) markers were utilized to study phylogenetic relationships among the taxa in the Polygonatum odoratum complex (Ruscaceae). European P. odoratum individuals form a clade with a high bootstrap value, which is a sister to the clade of Korean P. odoratum var. odoratum, P. odoratum var. pluriflorum and P. robustum. The formation of the clade with P. odoratum var. odoratum, P. robustum, and one accession of P. odoratum var. pluriflorum indicates geological speciation in isolated populations in the islands following dispersal events from the mainlands. All data sets form two major clades, which are congruent with the subgroups divided by the basic chromosome numbers (x = 9 and x = 10). Although it is not easy to test the hypothesis of the decrease in the basic chromosome number due to scatter taxon sampling in this study, the molecular data strongly suggested that aneuploidy plays an important role in lineage diversification in the genus Polygonatum. The cytological data was not strongly supported by the cpDNA sequences. Further investigations of the cytological, morphological, and geographical characteristics with comprehensive sampling are desired to understand the evolution and lineage diversification in the genus.

Studies on the Physiological and Ecological Characteristics of Red Rice($\underline{Oryza sativa}$L.) Collected in Korea 1. Degree of Grain shedding and Histological Peculiarities of Abscission Region Between Spikelet and Pedicel (우리나라 자생벼 수집 보존계통들의 생리 생태학적 특성에 관한 연구 I. 탈립성정도의 품종간 차이와 이층조직의 해부 형태학적 특성과의 관계)

  • Lim Won-Jae;Choi Kwang-Kwi;Jin Il-Doo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1989.10a
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    • pp.26-27
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    • 1989
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한국산 미꾸리과 어류, 종개의 분류학적 재검토

  • Kim, Ik-Soo;Park, Jong-Young;Yang, Hyun
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.481-482
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    • 2000
  • 미꾸리과(Cobitidae)에 포함되는 종개아과(Nemacheilinae)어류는 유우라시아에 분포하는 저서성 담수어류로 200여종이 알려졌지만 종에 관한 분류학적 연구가 많지 않기 때문에 계통적인 분류가 거의 불가능한 실정이다 (Kottelat, 1990). 한국산 종개아과에는 종개와 쌀미꾸리 2종이 기록되었지만 연구자에 따라 종개의 학명을 Oreiastoni (Jordan and Metz, 1913), Barbatula toni (Mori, 1930; Uchida, 1939; Chyun 1977), Nemacheilus toni (김 등, 1988; Choi et al., 1990; 양 등, 1991), Orthrias nu((Kim, 1999) 사용하고 있어 검토가 요구되었다. (중략)

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촉수과 어류의 비교해부학적 및 계통분류학적 연구 I. 촉수과 어류는 단계통군인가\ulcorner

  • 김병직
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.545-546
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    • 2001
  • 촉수과 어류는 농어목의 농어아목에 속하며, 세계의 온대 및 열대해역에 비교적 널리 분포하는 저서성 어류이다. 본과에는 노랑촉수속 Upeneus, Mulloidichthys속, 촉수속 Parupeneus, Mullus속, Upeneichthys속, 그리고 Pseudupeneus속의 6속에 약 55종이 알려져 있으며, 산업적인 중요종도 포함되어 있다(Nelson, 1994). 본과 어류는 하악봉합부의 직후에 새조골에서 기원한 1쌍의 긴 수염이 있는 점, 등지느러미가 2개로 서로 분리되어 있는 점, 그리고 꼬리지느러미가 깊게 이차하는 점 등의 특징을 갖는다. (중략)

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Wild Morus Survey in Korea (한국산 야생 Morus (뽕나무속) 탐견기)

  • 안학수
    • Journal of Sericultural and Entomological Science
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    • v.3
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    • pp.73-74
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    • 1963
  • 우리나라 Morus(뽕나무속)에 대한 분류학적인 연구는 지금으로부터 약 40 여년전부터 시작되어 현재는 거의 정리되었다고 볼 수 있다. 우선 우리나라에 자생되는 각종에 대하여 개별적인 분류지리학적면을 탐견 약기하여 보기로 한다. Morus alba L. 즉 재배종이며 보편적인 "뽕나무"로 알려져 있는 것이며 수많은 변, 품종들이 여기서 파생되고 있다. 한국산 야생 Morus를 크게 나누어 세가지 계통으로 구분할 수있다. 즉 I. Morus bombycis Koidzumi. 산뽕나무(Yamaguwa). II. Morus mongolica Schneider. 몽고뽕나무(Mongoguwa). III. Morus tiliaefolia Makino. 참털뽕나무(Keguwa). (중략)

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