• Title/Summary/Keyword: 계통육종

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Complete Mitochondrial Genome Sequence and Genetic Diversity of Duroc Breed (돼지 Duroc 품종에서 미토콘드리아 유전체 서열의 특성과 집단의 유전적 다양성)

  • Cho, 1.C.;Han, S.H.;Choi, Y.L.;Ko, M.S.;Lee, J.G;Lee, J.H;Jeon, J .T
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.937-946
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    • 2004
  • Duroc is widely used to improve the meat quality and productivity. To elucidate the phylogenetic relation and the sequence specificity for the maternal property, the complete sequence of mitochondrial genome was determined and the population diversity of Duroc was investigated in this study. The length of mtDNA tested is 16,584-bp. There are several insertion/deletion mutations in the control region and coding regions for tRNA and rRNA, respectively, but not in peptide-coding regions. Four peptide-coding genes(COⅡ, COⅢ, ND3 and ND4) showed incomplete termination codon sequences such as T--, and two(ND2 and ND4L) did alternative initiation codons(AIC), respectively. Especially, the initiation codon sequences of ND2 gene were polymorphic in this population. Polymorphisms were detected in 11-bp duplication motif within control region as well as ND2 and CYTB. Variation patterns observed from the tests on three mtDNA regions were linked completely and then two haplotypes obtained from combining the data dividing this population. Duroc mtDNA is observed at the European pig cluster in the phylogenetic tree, however, the results from the population analyses supported previous opinions. This study suggests that the breed Duroc was mainly originated from the European pig lineage, and Asian lineage was also used to form the pig breed Duroc as maternal progenitors.

Analysis of Genetic Variation of Perilla frutescens var. crispa Germplasm Using RAPD (RAPD를 이용한 차조기(Perilla frutescens var. crispa) 유전자원의 유전적 변이 분석)

  • Kim, Hyeun-Kyeung;Cho, Young-Son;Yang, Jae-Wan;Choi, Young-Whan;Kang, Jun-Soon;Lee, Yong-Jae;Son, Beung-Gu
    • Journal of Life Science
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    • v.20 no.1
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    • pp.119-123
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    • 2010
  • Genetic variations of Chajogi (Perilla frutescens var. crispa) germplasms were investigated by using RAPD markers. Twenty-two Perilla frutescens var. crispa lines collected from various locations were subjected to RAPD analysis using 80 primers. Among them, only 22 primers showed polymorphic bands and these 22 primers provided a total of 224 bands consisting of 127 polymorphic and 97 monomorphioc bands. The polymorphic bands were subjected to phylogenetic analysis using the UPGMA method. From UPGMA, similarity co-efficiency of 22 Chajogi lines ranged from 0.72 to 0.94. The dendrogram of 22 lines obtained through the UPGMA method resulted in two groups (one major group and one minor group). Although the two groups were roughly consistent with growth phenotypes (period of flowering, period of maturity, stem length, number of branches, number of nodes, number of flower clusters and number of ovaries) in detail, much inconsistency also was present

Phylogenetic Relationships of Mushroom Agrocybe spp. Based on rDNA-ITS Analysis (rDNA-ITS분석에 의한 볏짚버섯 (Agrocybe spp.) 수집균주의 계통분류학적 특성구분)

  • Cheong, Jong-Chun;Lee, Myung-Chul;Jang, Kab-Yeul;Jhune, Chang-Sung;Lee, Chan-Jung
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.11-18
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    • 2009
  • Phylogenetic relationships were investigated in the mushroom genus Agrocybe based on sequence data from the internal transcribed spaces(ITS) of nuclear ribosomal DNA(rDNA). Thirty strains including Agrocybe aegerita, A. grocybe cylindracea, A. grocybe praecox, A. grocybe pediades and Unknown Agrocybe species were subjected for the analysis. The Agrocybe spp. were separated into eight distinct groups. Phylogenetic analysis divided these species into eight groups as follows: A. chaxingu within group I, A. salicacola within group II, A. cylindracea and A. aegerita within group III, Agrocybe sp. within group IV and V, and other Agrocybe spp. were grouped within VI, VII, and VIII.

Characteristics of Flower and Fruit in Collected Schizandra chinensis BAILLON (오미자(五味子) 수집종(蒐集種)의 꽃과 과실(果實) 특성(特性))

  • Chang, Yeong-Hee;Park, Chun-Gun;Kim, Dong-Hwi
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.35-39
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    • 1995
  • Characteristics of Schizandra chinensis BAILLON collected were investigated the development of the new varieties and the results obtained are as follows: Flowering time of Schizandra chinensis varieties were varied from May 6 to 15. In petal base colors 70% were light pinked-colored, 23% were dark pinkcolored and 23% were white-colored respectively and number fruit set per node are 2 to 3 and 60% of plants were flower set and length of diameter of female flower longer than those of male flowers. Among 155 plants investigated, 125 plants showed below 40% of fruit setting and average fruit setting were 26%, 83% of plants were below 12mm, white 17% were above 12.1mm in fruit length, 100 dry fruit weight was 13.2g on average showing significant variation among plants. Average values of major characteristics of populations of collected varieties were 82.5 in number of fruit set, 68.3 in number of fruits 68.3mm in length of fruit set, 22.8mm in width of fruit set, 10.7mm in length of granule 103.1g in dry fruit weight per plants and ratio of dry fruit was 26.8% respectively.

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Genome size of 15 Lamiaceae taxa in Korea (한국산 꿀풀과 15 분류군에 대한 유전체양 조사)

  • Lee, Yoonkyung;Kim, Sangtae
    • Korean Journal of Plant Taxonomy
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    • v.47 no.2
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    • pp.161-169
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    • 2017
  • The genome size is one of the basic characters of an organism, and it is widely applied in various fields of biology, such as systematics, breeding biology, population biology, and evolutionary biology. This factor was recently highlighted in genome studies because choosing a representative of a plant group having the smallest genome size is important for the efficiency of a genome project. For the estimation of the genome size, flow cytometry has recently been highlighted because it is a convenient, fast, and reliable method. In this study, we report the genome sizes of 15 taxa of Lamiaceae from nine genera distributed in Korea using flow cytometry. Data pertaining to the genome size for all of our species have not been reported thus far, and the data from Agastache, Clinopodium, Elsholtzia, and Isodon are the first reported for each genus. The genome sizes of 15 genera and 39 species were reported to the Plant DNA C-values Database (http://data.kew.org/cvalues/). Scutellaria indica L. has a genome size of 0.37 pg (1C). This is the fourth smallest value among the 98 Lamiaceae taxa in the Angiosperm DNA C-value Database, indicating that this taxon can be used as a reference species in the genome studies in Lamiaceae as a native Korean species. The largest genome size observed in this study is in Phlomis umbrosa Turcz. (1C=2.60 pg), representing the possible polyploidy origin of this species in the family.

Analysis of Combining Ability for Quality and Yielding Characteristics in Rapeseed (Brassica napus L.) (유채의 품질과 수량 특성에 대한 조합능력 분석)

  • Shin, Dong-Young;Kwon, Byoung-Sun;Jung, Dong-Soo;Ham, Young-Soo;Lee, Jung-Il;Jang, Young-Seok;Chung, Dong-Hee;Park, Hee-Jin;Bae, Chang-Hyu;Hyun, Kyu-Hwan;Kuk, Yong-In;Choi, Gab-Lim;Yun, Young-Beom
    • Korean Journal of Plant Resources
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    • v.25 no.1
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    • pp.24-30
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    • 2012
  • Naehanyuchae and Youngsanyuchae were originated from the cross between "Erra(female)" and "Tower(male)". The cultivars were made at Bioenergy Crop Research Center, National Institute of crop science, Rural Development Administration, Korea in order to develop the high cold tolerant and yielding rapeseed variety with non-erucic acid and non-glucosinolate as good seed quality indicator. The superiorities of these varieties have been shown through the regional performance trials and demonstration farmers fields. Naehanyuchae and Youngsanyuchae had good agronomic characteristics related to yield potential such as many valuable branches and pods, and long pod length. The seeds of these new varieties composed of the 80% of oleic acid and linoleic acid without erucic acid, and glucosinolate was not extracted in the oil cake. The average grain yields increased by the from 16% to 22% according to the regional performance trials compared to check variety.

Analysis of Quantitative Trait Loci for Yield Component Traits in Soybean Using Recombinant Inbred Lines (콩에서 수량구성요인과 관련된 양적형질유전자좌의 분석)

  • Kim, Hyeun-Kyeung;Oh, Ki-Won;Choi, In-Soo;Kang, Jum-Soon;Choi, Young-Whan;Lee, Yong-Jae;Park, Young-Hoon;Son, Beung-Gu
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.599-605
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    • 2007
  • Seed and pod numbers are the main yield components in soybean. Selection for increased yield potential is main goal of plant breeding. The objective of this study was to identify quantitative trait loci(QTLs) that control pod number per plant, seed number per plant and pod in soybean. The 117 $F_{2:10}$ recombinant inbred lines(RILs) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Two independent QTLs for pod number per plant were identified from linkage group(LG) F and L. Two QTLs for seed number per plant were located on LG F and L. Seed number per pod was related with three QTLs located on LG D1a, D1b and F. Pod and seed number per plant have two common QTLs on LG F and L.

Simple Mass-screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 간편 대량 저항성 검정법)

  • Park, Myung Soo;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.110-116
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    • 2013
  • This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

Breeding of a New Late-season Pear Cultivar 'Mansoo' with Large Sized High Quality and Long Storability (저장력 강한 고품질 대과 만생종 배 '색수(晩秀)' 육성)

  • Kim, Whee-Cheon;Hwang, Hae-Sung;Shin, Il-Sheob;Shin, Yong-Uk;Lee, Don-Kyun;Kang, Sang-Jo;Moon, Jong-Youl;Kim, Jung-Ho
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.66-70
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    • 2001
  • 'Mansoo' is a late-season pear cultivar with extra large and high quality fruit of long storability, which was released by National Horticultural Research Institute in 1995. The cultivar originated from the cross between 'Danbae' and 'Okusankichi' at Suwon in 1978, had been under regional adaptability test at nine areas in the name of 'Wonkyo Na-15' for 4 years since 1992. It was vigorous in tree growth and upright in tree shape. Its flower bud is easily maintained, resulting in higher productivity than 'Danbae' and 'Okusankichi'. It bloomed a day later than 'Niitaka', and showed cross-compatibility with 'Niitaka', 'Chojuro' and some other varieties. Harvesting time of 'Mansoo' is late October in Suwon. The fruit shape is oblate and skin color is light yellowish brown. The fruit weighed 600-700 g and has 12-13% soluble solids content. The flesh is soft, juicy, and has negligible grit. It is resistant to black leaf rot (Alternaria kikuchiana Tanaka).

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'Picnic', a New Mid-season Apple Cultivar with Medium Size and Good Taste (식미가 우수한 중과형 사과 '피크닉' 육성)

  • Kwon, Soon-Il;Park, Jong-Taek;Lee, Jung-Woo;Kim, Mok-Jong;Kim, Jeong-Hee
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.784-788
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    • 2015
  • A new cultivar 'Picnic' originated from an artificial cross between 'Fuji' and 'Sansa' at National Institute of Horticultural & Herbal Science in 1994. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2003. After regional adaptability tests in five districts for four subsequent years as 'Wonkyo Ga-34', it was ultimately selected in 2008. Optimum harvest time is late September. Mature fruit has mean weight of 233 g and is conic with light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains a favorable total soluble solids content at $13.8^{\circ}Brix$ and titratable acidity at 0.43%, which results in gustatory harmony between sugars and acids. It is not resistant to bitter rot or Marssonina blotch. 'Picnic' exhibits a physiological cross compatibility with leading cultivars such as 'Fuji', 'Hongro' and 'Tsugaru'. Tree topology is semi-spreading with a weak growth habit.