• The Korean Journal of Mycology
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• v.35 no.1
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• pp.47-53
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• 2007

In this study, we investigated the properties of incubation and growing of Pleurotus eryngii in addition to the mycological properties to use them as basic data for breeding. The speed of mycelial growth on the MCM was faster than on the PDA. The biomass in the PDB broth culture was higher than in the MCM and YMG broth culture. KNR2515 and KNR2516 required 19 days for growth of mycelia on commercial sawdust media. KNR2503 required 6.5 days and 15.3 days for pin-heading and harvesting, respectively. In morphological properties by the mushroom, the heights of KNR2312 and KNR2322 were 122.7 and 121.0 mm, respectively. The thickness of KNR2322 and KNR2513 were 39.8 mm and 31.3 mm, respectively. The weight of KNR2524's fruiting body was 36.3 g, which is good as wild strain. The quality of fruiting body of KNR2503 was 4.0 in comparison to the score 7 of commercially cultivated strains. KNR2512 had the darkest color of pileus with L value 43.6. The slow growing strains, KNR2511, KNR2513, and KNR2512 had the bright pileus with L value 80. In morphological characteristics, KNR2511, KNR2513, and KNR2515 had white lamellar and plane pileus. The three strains are supposed to be the same group and KNR2516 and KNR2518 appeared to be related to the group. The commercially cultivated strains had convex pileus, KNR2502, KNR2503, KNR2504, KNR2521, and KNR2525 had infundibuliform, and the other strains had plane pileus. Several strains were valuable for breeding, JNR2503 for growth rate, KNR2512 for pileus color, and KNR2312, KNR2322, KNR2503, and KNR2513 for the quality.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.333-338
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• 2008

The tea has traditionally been used as a foodstuff by unique flavor, however recently not only the diversity of consumer demands but also the public interest in unique favorite and functional aspects have increased. It has been also reported that the main components contained in the leaves of tea (Camellia sinensis) include total nitrogen, free amino acids, polyphenols, and fiber, of which catechin has powerful bioactive effect such as anti-cancer, anti-aging, and anti-diabetic. (-)-Epigallocatechin gallate (EGCG) which is a major phenolic constituent of green tea extract has received considerable attention for a variety of important bioactivities. This study was carried out to obtain useful information for tea breeding programs, and to investigate the concentration of quality and functional related components in Korean indigenous tea germplasms. Korean indigenous tea lines were classified into three groups of sprout time, i.e, early, medium and late sprout time, and the ratio were 20%, 43% and 37%, respectively. There was a difference in characteristics among these Korean indigenous tea lines, leaf width of those ranged from 19.8 to 75 mm, leaf length was 35.5-160.0 mm, and leaf area was $660-8,400\;mm^2$. Experimental data on chlorophyll content (SPAD value) of Korean indigenous tea genetic resources ranged from 51.3 to 82.3. The concentrations of the total nitrogen, total free amino acids, and theanine were ranged 4.18-6.07%, 2.87-4.58%, and 1.64-2.66%, respectively. Also, catechin concentration showed from 11.54 to 15.07%, and concentration of caffeine was 2.82-4.23%. These results indicated indicated that it is possible to select elite lines with high concentration of quality related components and low concentration of caffeine from Korean domestic tea germplasms.

• Journal of the Korean Society of International Agriculture
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• v.31 no.4
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• pp.428-436
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• 2019

As the main objective of rice breeding programs, rice eating quality is one of critical factors directly determining the market price and the consumer preference. However, the genetic complexity of eating quality and the difficulty in accurate evaluation often constrain improvement of the eating quality in rice breeding programs. In addition, given that the rice eating quality of current cultivars has already reached some high-level, diversifying of genetic resources are demanded more than ever to improve the rice eating quality. In this study, we developed a recombinant inbred lines (RILs) population derive from Wandoaengmi6, a japonica-type Korean weedy rice with high eating quality and a high degree of glossiness of cooked rice. Year-to-year correlations between the traits in three years were shown normal distribution for major agronomic traits and physicochemical characteristics. After evaluating tested traits related to eating quality procedure, a total of ten lines were ultimately selected from the population. Increasement of the taste of cooked rice (TA) and the overall eating quality (OE) were confirmed in the selected lines, which are caused by alleles derived from Wandoaengmi6 without any linkage drag. These results indicate that the utility of wide genomic resources in Korean landrace could be of application in various rice breeding programs and countermeasure to contribute to properly response to climate change.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.277-286
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• 1985

From a series of studies conducted on the local maize lines at the Agr. College of the Chungnam National Univ., a few maize lines with many tillers and ears per plant were identified and tentatively named as MET. The purpose of the study was to evaluate the MET lines, which were selfed for five generations, for silage purpose under the different plant densities. A hybrid, Suwon #19 and a synthetic variety, Puyo #3${\times}$#2, were included for comparison. Plant height at harvesting times showed no significant varietal differences. However, the MET lines were very slow in early plant growth compared to the hybrid or synthetic variety, probably due to inbreeding depression of the MET lines. Total fresh weight at the harvesting times was highest in the MET 1 line. The MET 1 line was about 2,000 kgr. per 10a. higher than the hybrid at the harvesting time. The highest fresh weight was obtained when grown under the plant density of 60 ${\times}$ 20cm. Total dry weight per unit area showed the same tendency as the fresh weight. Total dry weight of MET 1 line was about 2.4 tons per 10a., which was about 10% higher than the hybrid, Suwon #19. As the fresh weight, the total dry weight was also highest in the plant density of 60 ${\times}$ 20cm. The grain yield per 10 are of MET 1 was comparable to the grain yield of the hybrid, especially in the low plant density, 60 ${\times}$ 40cm. The average number of effective tillers of MET lines were 4.5, while the mean tiller number of the hybrid or synthetic variety were none. However, the lodging was one of the problem for growing MET lines. The 100 kernel weight of MET lines was about 9 gr., while the 100 kernel weight of the hybrid or synthetic variety was about 30 grm.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.151-156
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• 2003

The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.143-149
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• 2003

This study was carried out to cryopreserve and to investigate characteristics of semen in Hanwoo. Semen was obtained from bulls selected by Daekwanryeong Branch station. Semen was collected each morning of the experiment, placed in water jacketed tubes at 37$^{\circ}C$, and trans-ported to the research laboratory within 10 minutes. Semen was extended with Egg yolk-glycerol extender to contain 50${\times}$10$^{6}$ sperm/ml. Semen was cooled over a 6h period in water jacketed tubes from about 25 to 5$^{\circ}C$, Egg yolk-glycerol extender was added in one step at 5$^{\circ}C$. Semen was aspirated into 0.5ml straws, which were sealed with powder. Egg yolk-glycerol extender, which is used in Hanwoo sperm frozen and stored, semen from 13 Hanwoo bulls collected, the postthawed percentages of motile sperm were 65.7%. In semen characteristics of Hanwoo bulls, number of bulls volume are 5.7 ml and total cell count are 975${\times}$10$^{6}$ m1 ejaculate.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.25-29
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• 1995

In order to obtain the basic information on cross breeding of peony, the growth and floral characteristics were investigasted for local collected strains in main cultvation area in Korea. Local peony strains were classified by early emergence lines and late emergence lines according to the emergence date. Flower color of single-petaling flower was varied with red, pink and white, on the other hand, red+red, pink+white, pink+red and pink+pink in double-petaling flower. Number of pistil of the most local peony strains was 3-4/flower, number of stamen was varied from 0 to more than 200 ea/flower, classifing by 4 groups according to the diameter of flower. Most of the cross combinations showed the cross comptibility, and number of follicle was 3-5 ea/flower, average number of seed was 15.6 ea/follicle, seed size was grouped : by small grains with less than 10g, medium grains with 10-15g and large grains with more than 15g of 100 seed weight.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.722-729
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• 2015

Powdery mildew (PM) caused by Podosphaera aphanis is a major disease that can result in significant yield losses in strawberry (Fragaria ${\times}$ ananassa Duchesne). For preventing PM, pesticides are usually applied in strawberry. In this study, molecular markers were developed to increase breeding efficiency of PM-resistance cultivars by marker-assisted selection (MAS). An $F_2$ population derived from a cross between PM-resistance 'Seolhyang' and PM-susceptibility 'Akihime' was evaluated for disease resistance to PM and RAPD (random amplification of polymorphic DNA)-BSA (bulked segregant analysis). Among 200 RAPD primers tested, OPE10 primer amplified a 311bp-band present in with 331bp. Sequence alignment performed for searching polymorphisms and six single nucleotide polymorphism (SNP) were found in amplified regions. To develop polymorphic marker for distinguishing between resistant and susceptible, RAPD was converted to cleaved amplified polymorphic sequence (CAPS) marker. Among restriction enzymes associated with six SNPs, Eae I (Y/GGCCR) was successfully digested to 231bp in susceptible. The results suggest that the selected CAPS marker could be used for increasing efficiency of selecting powdery mildew resistant strawberry in breeding system.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.306-309
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• 2009

A New bicolor standard rose (Rose hybrida Hort.) cultivar were developed by line selection in 2000 at the Flower Research Institute, Gyeongnam ARES. Characteristics were investigated three times from 2003 to 2006 "Grandking" was crossed between 'Bixa' and 'Red Sandra' and it has a red-group color (RHS No. 52A), few thorns and standard large flower with a good harmony between ray floret and flower center. The vase life of cut flower was 12.8 days. The major characteristics of this variety are 228 stems/$m^2$/year in yield, 77.4 cm in length of cut flower, 9.0 cm in flower diameter, and 36.1 in petal number. It was registered as commercial cultivar in 2006. These new variety could be planted in almost greenhouse production region of Korea.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.342-345
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• 2009

"Hwaweon 2" was developed from an interspecific cross between Hwaseongbyeo and a wild species, Oryza rufipogon L. (IRGC 105491) based on marker-aided selection. The recurrent parent "Hwaseongbyeo" is a high grain quality cultivar with medium-maturity. Hwaweon 2 is nearly isogenic to Hwaseongbyeo except a small O. rufipogon introgression on chromosome 9. This segment was associated with genes controlling a number of traits including grain weight, heading date, culm length, and spikelets per panicle. The preliminary and replicated yield trial was conducted at Chungnam National University in 2004, 2005 and 2006. The local adaptability test was carried out by the National Seed Management Office (NSMO) in 2007 and 2008. This cultivar was registered to NSMO with a cultivar designated as "Hwaweon 2". This cultivar averaged 98cm in culm length and has a medium to late growth duration. This variety is resistant to stripe virus as the recurrent parent. Milled rice of "Hwaweon 2" is translucent and the grain quality traits are comparable to those of the recurrent parent. The yield potential of "Hwaweon 2" in grain is about 7.68 MT/ha at the ordinary fertilizer level about 14% higher than that of Hwaseongbyeo due to increase in grain weight and spikelets per panicle.