• Title/Summary/Keyword: 계대

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Change in the RAPD Band of Strawberry Depending on 2,4-D and NAA Treatment and the Number of Subcultures In Vitro (기내배양에서 2,4-D 및 NAA처리와 계대배양회수에 따른 딸기의 RAPD Band 변화)

  • Shim, Jai-Sung;Chung, Hae-Joon;Min, Byung-Hoon
    • The Journal of Natural Sciences
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    • v.8 no.2
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    • pp.147-152
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    • 1996

  • The 2,4-D + BA combination in MS medium showed high regenerating ability and induced compact callus from leaf and petiole segments of three strawberry cultivars, 'Bokyo', 'Suhong', whereas the NAA + BA combination resulted in friable callus. Band of RAPD products obtained with primer 212 from the callus was different from the band of mother plant when callus induced from leaf segment of 'Suhong' cultivar was maintained in MS medium containing NAA or BA for 8 months. The RAPD bands obtained from mother plants of 'Bokyo' and 'Yeobong' were different from that of callus maintained in the presence of MS liquid medium containing 2,4-D(0.2 mg/$\ell$) subcultured every two weeks for 6 months(12 subcultures).

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Gamma Radiation-Induced Changes of Antioxidant Enzymes in Callus Cultures of Cassava(Manihot esculenta Crantz) (감마선에 의한 카사바 (Manihot esculenta Crantz) 배양세포의 항산화효소 활성 변화)

  • 이행순;유순희;권석윤;김재성;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.53-58
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    • 1999

  • The gamma radiation-induced changes of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in callus cultures of cassava (Manihot esculenta Crantz) selected as a high yield of cell line for SOD were investigated. In normal cultures, the cell growth reached a maximum at 30 days after subculture (DAS), followed by a rapid decrease with further cultures. The SOD and POD specific activities (units/mg protein) showed the highest at the immediately after subculture and subsequently decreased to 20 DAS, and then increased to 30 DAS, whereas the CAT activity showed the lowest at just after subculture, and it continuously increased from 15 DAS to 30 DAS, showing a good correlation with the cell growth. Irradiation of gamma-ray of 50 and 70 Gy on 7 DAS inhibited significantly the cell growth by 50% and 80% at 14 days after treatment (DAT), respectively. In the cells irradiated with 70 Gy, SOD and POD specific activities increased by 4 and 2.5 folds at 14 DAT, respectively, whereas CAT activity was not affected. The results indicate that SOD and POD may be involved in the antioxidative mechanism in relation to oxidative stresses induced by subcultures and by gamma radiation in callus cultures of cassava.

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A simple mid-term preservation method (SMPM) of plant callus under low temperature conditions (저온 보존을 이용한 간편 중기 식물캘러스 저장법)

  • Park, Sung-Chul;Park, Su Hyun;Kim, Soyoung;Jeong, Yu Jeong;Kim, Cha Young;Jeong, Jae Cheol
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.187-192
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    • 2022

  • The repeated monthly or weekly subculture of plant callus is labor intensive and increases the risk of somaclonal variation from the parental callus line. The most effective method for preserving plant callus is cryopreservation, which involves storage in liquid nitrogen. However, this method cannot be applied to the callus of different plant species in the same manner, so it is difficult to develop a standardized cryopreservation method. In addition, the survival rate of the frozen callus after thawing and the regeneration rate after survival are uncertain. Therefore, it is necessary to develop a method to extend the subculture interval of plant callus in an active state. In this study, active plant calli of various species without freezing was incubated at 15℃ for 4 to 12 weeks without subculture. After 12 weeks, 8 lines of plant callus grew less than 2-fold when cultured at 25℃, but at least 2 times as much when cultured at 15℃. Moreover, total antioxidant activity did not differ significantly between plant callus recovered at 25℃ after culturing at 15℃ or at 25℃. These results show that the subculture interval can be extended at a temperature of 15℃ without need for modified medium composition or additional processes. In addition, positive results in all calli of several plant species are expected to reduce labor as well as somaclonal variation by increasing the subculture.

  • https://doi.org/10.5010/JPB.2022.49.3.187 인용 PDF KSCI